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- EMDB-14798: CryoEM structure of mitochondrial complex I from Chaetomium therm... -

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Basic information

Entry
Database: EMDB / ID: EMD-14798
TitleCryoEM structure of mitochondrial complex I from Chaetomium thermophilum (state 1) - membrane arm
Map dataDensity modified map (Terwilliger et al., 2020)
Sample
  • Complex: Mitochondrial NADH:ubiquinone oxidoreductase in LMNG
    • Protein or peptide: x 26 types
  • Ligand: x 6 types
Function / homology
Function and homology information


single-stranded DNA helicase activity / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / electron transport chain / NADH dehydrogenase (ubiquinone) activity / ATP synthesis coupled electron transport / respirasome / mitochondrial membrane ...single-stranded DNA helicase activity / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / electron transport chain / NADH dehydrogenase (ubiquinone) activity / ATP synthesis coupled electron transport / respirasome / mitochondrial membrane / mitochondrial intermembrane space / fatty acid biosynthetic process / 4 iron, 4 sulfur cluster binding / DNA helicase / mitochondrial inner membrane / DNA replication / hydrolase activity / DNA repair / mitochondrion / DNA binding / ATP binding / membrane / metal ion binding / nucleus
Similarity search - Function
NADH-ubiquinone oxidoreductase 17.8kDa subunit / : / Dna2 Rift barrel domain / DNA replication factor Dna2, N-terminal / DNA replication ATP-dependent helicase/nuclease Dna2 / DNA replication factor Dna2 / Dna2/Cas4, domain of unknown function DUF83 / Domain of unknown function DUF83 / NADH-ubiquinone oxidoreductase, 21kDa subunit, C-terminal, fungi / NADH-ubiquinone oxidoreductase 9.5kDa subunit ...NADH-ubiquinone oxidoreductase 17.8kDa subunit / : / Dna2 Rift barrel domain / DNA replication factor Dna2, N-terminal / DNA replication ATP-dependent helicase/nuclease Dna2 / DNA replication factor Dna2 / Dna2/Cas4, domain of unknown function DUF83 / Domain of unknown function DUF83 / NADH-ubiquinone oxidoreductase, 21kDa subunit, C-terminal, fungi / NADH-ubiquinone oxidoreductase 9.5kDa subunit / C-terminal of NADH-ubiquinone oxidoreductase 21 kDa subunit / NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit / : / DNA2/NAM7 helicase, helicase domain / DNA2/NAM7-like helicase / AAA domain / PD-(D/E)XK endonuclease-like domain superfamily / NAD(P)H-quinone oxidoreductase, subunit N/subunit 2 / NADH:ubiquinone oxidoreductase, ESSS subunit / ESSS subunit of NADH:ubiquinone oxidoreductase (complex I) / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 / GRIM-19 / NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8) / GRIM-19 protein / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase subunit 5, C-terminal / NADH dehydrogenase subunit 5 C-terminus / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7 / NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7) / NDUFB9, LYR domain / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ / Complex 1 LYR protein domain / NADH-plastoquinone oxidoreductase, chain 5 subgroup / NADH-quinone oxidoreductase, chain M/4 / Complex 1 protein (LYR family) / NADH-ubiquinone oxidoreductase chain 4L/K / NADH:ubiquinone/plastoquinone oxidoreductase, chain 6 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6 / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminal / NADH-quinone oxidoreductase, chain 5-like / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus / NADH-ubiquinone oxidoreductase chain 4L/Mnh complex subunit C1-like / NADH-ubiquinone/plastoquinone oxidoreductase chain 4L / NADH:ubiquinone/plastoquinone oxidoreductase, chain 3 / NADH:ubiquinone oxidoreductase, subunit 3 superfamily / NADH-ubiquinone/plastoquinone oxidoreductase, chain 3 / NADH:ubiquinone oxidoreductase, subunit 1, conserved site / Respiratory-chain NADH dehydrogenase subunit 1 signature 1. / Respiratory-chain NADH dehydrogenase subunit 1 signature 2. / NADH:ubiquinone oxidoreductase, subunit 1/F420H2 oxidoreductase subunit H / NADH dehydrogenase / CHCH / CHCH domain / NADH:ubiquinone oxidoreductase / NADH:quinone oxidoreductase/Mrp antiporter, membrane subunit / Proton-conducting membrane transporter / Coiled coil-helix-coiled coil-helix (CHCH) domain profile. / Acyl carrier protein (ACP) / Phosphopantetheine attachment site / Phosphopantetheine attachment site. / Phosphopantetheine attachment site / ACP-like superfamily / Carrier protein (CP) domain profile. / Phosphopantetheine binding ACP domain / DNA2/NAM7 helicase-like, C-terminal / AAA domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
NADH dehydrogenase (Ubiquinone)-like protein / NADH-ubiquinone oxidoreductase 9.5 kDa subunit / NADH-ubiquinone oxidoreductase-like protein / NADH-ubiquinone oxidoreductase / NADH-ubiquinone oxidoreductase-like protein / NADH-ubiquinone oxidoreductase-like protein / Uncharacterized protein / NADH-ubiquinone oxidoreductase B15 subunit / Uncharacterized protein / Uncharacterized protein ...NADH dehydrogenase (Ubiquinone)-like protein / NADH-ubiquinone oxidoreductase 9.5 kDa subunit / NADH-ubiquinone oxidoreductase-like protein / NADH-ubiquinone oxidoreductase / NADH-ubiquinone oxidoreductase-like protein / NADH-ubiquinone oxidoreductase-like protein / Uncharacterized protein / NADH-ubiquinone oxidoreductase B15 subunit / Uncharacterized protein / Uncharacterized protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / DNA replication ATP-dependent helicase/nuclease DNA2 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / Acyl carrier protein / NADH-ubiquinone oxidoreductase-like protein / Uncharacterized protein / NADH-ubiquinone oxidoreductase chain 6 / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 4L / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 1 / NADH-ubiquinone oxidoreductase chain 4
Similarity search - Component
Biological speciesChaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.47 Å
AuthorsLaube E / Kuehlbrandt W
Funding support Germany, 1 items
OrganizationGrant numberCountry
Max Planck Society Germany
CitationJournal: Sci Adv / Year: 2022
Title: Conformational changes in mitochondrial complex I of the thermophilic eukaryote .
Authors: Eike Laube / Jakob Meier-Credo / Julian D Langer / Werner Kühlbrandt /
Abstract: Mitochondrial complex I is a redox-driven proton pump that generates proton-motive force across the inner mitochondrial membrane, powering oxidative phosphorylation and ATP synthesis in eukaryotes. ...Mitochondrial complex I is a redox-driven proton pump that generates proton-motive force across the inner mitochondrial membrane, powering oxidative phosphorylation and ATP synthesis in eukaryotes. We report the structure of complex I from the thermophilic fungus , determined by cryoEM up to 2.4-Å resolution. We show that the complex undergoes a transition between two conformations, which we refer to as state 1 and state 2. The conformational switch is manifest in a twisting movement of the peripheral arm relative to the membrane arm, but most notably in substantial rearrangements of the Q-binding cavity and the E-channel, resulting in a continuous aqueous passage from the E-channel to subunit ND5 at the far end of the membrane arm. The conformational changes in the complex interior resemble those reported for mammalian complex I, suggesting a highly conserved, universal mechanism of coupling electron transport to proton pumping.
History
DepositionApr 19, 2022-
Header (metadata) releaseNov 30, 2022-
Map releaseNov 30, 2022-
UpdateDec 7, 2022-
Current statusDec 7, 2022Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_14798.png

Downloads & links

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Map

FileDownload / File: emd_14798.map.gz / Format: CCP4 / Size: 46.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationDensity modified map (Terwilliger et al., 2020)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesX (Sec.)Y (Row.)Z (Col.)
0.84 Å/pix.
x 172 pix.
= 143.964 Å		0.84 Å/pix.
x 261 pix.
= 218.457 Å		0.84 Å/pix.
x 272 pix.
= 227.664 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

generated in cubic-lattice coordinate

Voxel sizeX=Y=Z: 0.837 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.12
Minimum - Maximum-2.1241705 - 3.2206013
Average (Standard dev.)6.479937e-13 (±0.16787401)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin153100210
Dimensions261272172
Spacing172261272
CellA: 143.964 Å / B: 218.457 Å / C: 227.664 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: Unmodified/unsharpened map

Fileemd_14798_additional_1.map
AnnotationUnmodified/unsharpened map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: EM half map B

Fileemd_14798_half_map_1.map
AnnotationEM_half_map_B
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: EM half map A

Fileemd_14798_half_map_2.map
AnnotationEM_half_map_A
Projections & Slices
AxesZYX

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Sample components

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Entire : Mitochondrial NADH:ubiquinone oxidoreductase in LMNG

EntireName: Mitochondrial NADH:ubiquinone oxidoreductase in LMNG
Components
  • Complex: Mitochondrial NADH:ubiquinone oxidoreductase in LMNG
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 1
    • Protein or peptide: NADH dehydrogenase subunit 2
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 3
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 5
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 6
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
    • Protein or peptide: Subunit NDUFS5 of NADH-ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: Subunit NDUFA1 of NADH-ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NADH-ubiquinone oxidoreductase-like protein
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4L
    • Protein or peptide: Acyl carrier protein
    • Protein or peptide: Complex I-B22
    • Protein or peptide: Complex I-ESSS
    • Protein or peptide: NADH-ubiquinone oxidoreductase
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
    • Protein or peptide: NADH-ubiquinone oxidoreductase-like protein
    • Protein or peptide: NADH dehydrogenase (Ubiquinone)-like protein
    • Protein or peptide: Subunit NDUFC2 of NADH-ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: Subunit NDUFB3 of NADH-ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: Subunit NDUFB10 of NADH-ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: Subunit NDUFB2 of NADH-ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: Subunit NDUFA3 of NADH-ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: Subunit NDUFB6 of NADH-ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: Subunit NDUFB4 of NADH-ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: Subunit NDUFB5 of NADH-ubiquinone oxidoreductase (Complex I)
  • Ligand: 1,2-Distearoyl-sn-glycerophosphoethanolamine
  • Ligand: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
  • Ligand: CARDIOLIPIN
  • Ligand: Lauryl Maltose Neopentyl Glycol
  • Ligand: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate
  • Ligand: water

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Supramolecule #1: Mitochondrial NADH:ubiquinone oxidoreductase in LMNG

SupramoleculeName: Mitochondrial NADH:ubiquinone oxidoreductase in LMNG / type: complex / Chimera: Yes / ID: 1 / Parent: 0 / Macromolecule list: #1-#26
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Molecular weightTheoretical: 970 KDa

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Macromolecule #1: NADH-ubiquinone oxidoreductase chain 1

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 41.716406 KDa
SequenceString: MSYSQTINSL VEVVLVLVPS LVGIAYVTVG ERKTMGSMQR RLGPNAVGIY GLLQAFADAL KLLLKEYVGP TQANLVLFFL GPVITLIFS LLGYAVIPYG PGLAVNDLST GILYMLAVSS LATYGILLAG WSANSKYAFL GSLRSTAQLI SYELVLSSSI L LVIMLSGS ...String:
MSYSQTINSL VEVVLVLVPS LVGIAYVTVG ERKTMGSMQR RLGPNAVGIY GLLQAFADAL KLLLKEYVGP TQANLVLFFL GPVITLIFS LLGYAVIPYG PGLAVNDLST GILYMLAVSS LATYGILLAG WSANSKYAFL GSLRSTAQLI SYELVLSSSI L LVIMLSGS LSLTVIVESQ RAIWYILPLL PVFIIFFIGS VAETNRAPFD LAEAESELVS GFMTEHAAVI FVFFFLAEYG SI VLMCILT SILFLGGYLL ISLLDIIYNN LLSWIVIGKY IIFIFPFWGP VFIDLGLYEI ISYLYNAPTV EGSFYGLSLG VKT SILIFV FIWTRASFPR IRFDQLMSFC WTVLLPILFA LIVLVPCILY SFNIFPVNIS LL

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Macromolecule #2: NADH dehydrogenase subunit 2

MacromoleculeName: NADH dehydrogenase subunit 2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 64.12918 KDa
SequenceString: MIMISILSLL LSTSVTLRRD MSILFNRISI IALAYCILHD TMSLSFISKG IGLHGGLLHI TNLTQIFHIF IFIISILILQ LTSFYPRKV WIPEYSSLKD IFFNKILYYR TKIINKMGEH MKIIEYPLIL LFVISGAVFL ISTNDLVSIF LSIELQSYGL Y LLSTIYRN ...String:
MIMISILSLL LSTSVTLRRD MSILFNRISI IALAYCILHD TMSLSFISKG IGLHGGLLHI TNLTQIFHIF IFIISILILQ LTSFYPRKV WIPEYSSLKD IFFNKILYYR TKIINKMGEH MKIIEYPLIL LFVISGAVFL ISTNDLVSIF LSIELQSYGL Y LLSTIYRN SELSTTGGLI YFLLGGLSSC FILLGTSLLY VNSGTTSLDG LYILNSISDV NPVVAGVGED GGLTSWYKPY YL NFSLLIF SIGFLFKVSA APFHFWSPDV YDAIPTIVTT FVAIIAKISI FIFLLELVYY TNSNANSYLS EFSWTYALLI SSL LSLIIG TVVGLTQFRI KRLLAYSTIS HVGFILLALS VSSIESTQAF IFYLIQYSIS NLNAFFILIT IGFSLYGYVT NNKE YKSLL DKNNSPIQLI SQLKGYFYIN PLLSLSLAIT IFSFVGVPPL VGFFAKQMVL SAALDNGYIF LSLIAIITSV IGAVY YLNV IKEIFFYSPE HEVNPVLNES DSNFSLRILN EKNVLIRSVL LKGRNIFISS PFSITISIIT NVILLFIFMN KEWLSM GTI LVQILFSA

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Macromolecule #3: NADH-ubiquinone oxidoreductase chain 3

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 16.33003 KDa
SequenceString:
MSAMSIYIIF VSIIAILFLA IDLIFAPHNP YKEKLSAFEC GFHSFSQSRS PFNISFFIYG LVFLLLDLEI LLLYPFAVSE YVNSAYGLA AALIFIGIIT IGFVYELGHD ALKVHSRQNI STKDLKSSVV ISYLGNINND SVNLHIK

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Macromolecule #4: NADH-ubiquinone oxidoreductase chain 4

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 4 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 60.810309 KDa
SequenceString: MSLLYVLLII PIIGIFLIST IDSFYPVTNT NIVLNKKFFR GFNDARQPIK YLYFSEGESF NIENKEDSFF NVSYYKKIAL ITTILNLIV SLIIYILFDF SNNQFQFIQE NLDLSFYDIY LGVDGVSIYF VLLTTIIMPI ALVSNWNSIT NNIKSYLIIM L LLETLLLA ...String:
MSLLYVLLII PIIGIFLIST IDSFYPVTNT NIVLNKKFFR GFNDARQPIK YLYFSEGESF NIENKEDSFF NVSYYKKIAL ITTILNLIV SLIIYILFDF SNNQFQFIQE NLDLSFYDIY LGVDGVSIYF VLLTTIIMPI ALVSNWNSIT NNIKSYLIIM L LLETLLLA VFLVLDVLLF YIFFESILPP LFILIGLFGS SNKVRASFYI FLYTLLGSLF LLLSILTMSS IVGTTYFDVL LK SSFEYTT QLFLFFGIFI AFAVKTPVWG LNSWLLRAHV ESPLGGSIVL AAIVLKLSLY GVFRLILPIL PQASLNLTYI VYA IGAITV LYASFSTLRT VDVKELIAYS SVAHAAIYLM GVFSNTIQGL EGAILLGLAH GFVSSGLFIC AGGILYDRTG TRLI YFFRG LTQIMPLFSL FFFILCLGNA GTPLTLNFVG EFMSLYGTLE RLPIAGMLAS TSIIFSAAYS IYMYNRIAFG GSVSL YFID CFRDLTKREF FILFTLVSFT VILGIYPSFV LDGLHYNISS VVYGIEPNAS YLTQGGNL

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Macromolecule #5: NADH-ubiquinone oxidoreductase chain 5

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 5 / type: protein_or_peptide / ID: 5
Details: Discrepancy with annotated entry sequence. Probably wrong intron-exon boundaries. Amino acids added according to map density, genome sequence and MS data.
Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 75.872031 KDa
SequenceString: MYLSIIILPL LGSVVSGFFG RKVGVSGAQL ITCSSVIITT ILSIIAFFEV GFNNIPVTIN IFRWIDSEWF IINWGFQYDS LTVSMLIPV LIISSLVHIY SISYMSSDPH NQRFFSYLSL FTFMMIILVT ANNYLLMFVG WEGVGVCSYL LVSFWFTRIA A NQSSISAF ...String:
MYLSIIILPL LGSVVSGFFG RKVGVSGAQL ITCSSVIITT ILSIIAFFEV GFNNIPVTIN IFRWIDSEWF IINWGFQYDS LTVSMLIPV LIISSLVHIY SISYMSSDPH NQRFFSYLSL FTFMMIILVT ANNYLLMFVG WEGVGVCSYL LVSFWFTRIA A NQSSISAF LTNRVGDCFL TVGMFAILWS LGNLDYATVF SLAPYINSNV VIIIGICLLI GAMAKSSQVG LHVWLPMAME GP TPVSALI HAATMVTAGV YLLMRSSPLI EYSSTVLLLC LWLGAITTVF SSLIGLFQQD IKKVIAYSTM SQLGMMVLSI GLS SYNIAL FHLVNHAFYK ALLFLGAGSV IHAVADNQDF RKFGGLISYL PLTYSVMLIA SLSLVAFPFM TGFYSKDFIL ESAY GQFSF SGVAVYIIAT IGAIFTTLYS VKVLYLTFLS NPNGPRTYYR LAIDNFFSAQ AIKSYKPAHE GDFFLTLPLV ILALF SIFF GFITKDIFIG LGSNFFVDNS LFIHPIHEIM IDTEFAVPVL FKLLPFIFTI SFSVIALTLS ELLSELVIYF KFSRFG YNI FGFFNQRFLI EFFYNKYITN LILNLGGQIT KILDKGSIEL FGPYGLERGL VKLSKNISSL STSHVTTYAL YILVGFI LY LIYNNLLLDY SYLLLIIILL LLLMMIGESN SEDVTLH

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Macromolecule #6: NADH-ubiquinone oxidoreductase chain 6

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 6 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 24.819291 KDa
SequenceString: MNSQISLLLL KEIYTNGSTH IMLDILSVLA VISGICVIIS KNPIVSVLHL IGLFAYVSFY LILIGLNFVG LSYLIVYIGA VSILFLFIL MLINIRTSEL QSNTSNSIPL TILVGIIISS FLFKMLPYGV IISNQFNSSN LNENLYTIQI VGGEDNNINN I NTDKNDLF ...String:
MNSQISLLLL KEIYTNGSTH IMLDILSVLA VISGICVIIS KNPIVSVLHL IGLFAYVSFY LILIGLNFVG LSYLIVYIGA VSILFLFIL MLINIRTSEL QSNTSNSIPL TILVGIIISS FLFKMLPYGV IISNQFNSSN LNENLYTIQI VGGEDNNINN I NTDKNDLF FITSKIWDGA LAENNHISSI GNIMYTNYNV WLILASFILL LAMVGAIVIT IKPRKI

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Macromolecule #7: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 10.31275 KDa
SequenceString:
MATDAAETSR RATREEMRDA KVPLAYRDSC AHLLIPLNRC RYETYYLPWK CEDERHSYEK CQYLEFKKRV QKMEELREAK GGARSN

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Macromolecule #8: Subunit NDUFS5 of NADH-ubiquinone oxidoreductase (Complex I)

MacromoleculeName: Subunit NDUFS5 of NADH-ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 8
Details: Discrepancy with annotated entry sequence. Probably wrong intron-exon boundaries. One additional amino acid modelled according to map density and genome sequence.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 87.142328 KDa
SequenceString: MASGYGLNGG PSRCFPFWQE LLACYVTNSS EDNPDGKNKC IPVMEDYYEC LHHRKEAARV RALQAAYREA EAKKLQENPP TAGQIRNLG LLNKEEDTKK VHCATATQQW MKMCFALSAK MPCLRGIEVF LTTRPDNEKI PEYPHPEGTS ARVICGQHLI R SPSLASNL ...String:
MASGYGLNGG PSRCFPFWQE LLACYVTNSS EDNPDGKNKC IPVMEDYYEC LHHRKEAARV RALQAAYREA EAKKLQENPP TAGQIRNLG LLNKEEDTKK VHCATATQQW MKMCFALSAK MPCLRGIEVF LTTRPDNEKI PEYPHPEGTS ARVICGQHLI R SPSLASNL SNSAITPPSS FSPRKANPLV SVYLPSVPGT PFTINYKISQ VPPEPCKYLF FRLYINARPM VSWGIDPHSR PY GKVIKSL WLPSDDRYRG LVGFEKRSFV FLPGEEFKSV AEDGGLIEVQ VFRAKDRRAR TPKLETFRFR DNYGIAAPSI GLL DKPQNA FFYDWLLIDP KDEPFAKFRM HYRSWRNLKS LNLIPSSEWE LLLAVSPKAL RTAASTGKIE KPTSPAFSDS DSDD SLCSA TDSDECVFDD HSKKTKSNRS KESPFAFLNS PPERFRAMAP SSEKLPQPSK LLRDSQRAPY QSRPLPELPV EAGVN STGL NPPSVKVAAD LRRKPSATSM ESNAVSITPS LLRCMEEGTL DLEKAEVGIA KLVKVAASGS EPSSSSSSAT QLTVSA VRE VELRPPQPER KGGLPMDYSF SDYEKSSQSS FGDDERMSNI SDMEEKPFPA PPTCYLPTTG SGLERELAMF DSPSPSP VP YSAMEPPVTP SPSSTPYKTK LGRKPLLFSR RLGLFSPRKS LPSDFLLGQA KKLVVEEETT NSNVSLAFGE LTVTDMRA E SPSPAPKGKP LRRFSTIRVE EISIKEKRPL FNSLRRIASA SPRKLAGRVL SMDLGKKGGE EKEG

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Macromolecule #9: Subunit NDUFA1 of NADH-ubiquinone oxidoreductase (Complex I)

MacromoleculeName: Subunit NDUFA1 of NADH-ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 9
Details: No entry (not annotated). Sequence modelled according to map density, genome sequence and MS data. Last five amino acids unknown and modelled as Poly-Ala
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Molecular weightTheoretical: 9.833353 KDa
SequenceString:
MPVPFETLIP YGIIIAMFGV TGAGMAKVRH MFNGDKRHRW SVDQWDKQQM ERDRRLTGHL RGQTDNPIAP PGFEFNNPWK V(UNK)(UNK)(UNK)(UNK)(UNK)

+
Macromolecule #10: NADH-ubiquinone oxidoreductase-like protein

MacromoleculeName: NADH-ubiquinone oxidoreductase-like protein / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 21.612625 KDa
SequenceString: MAPIEEEHEH YHPKDAVHLG LKGAAVVGGI GLLFAAVRTS LARKNVGPWA IFTRNGKLAA TFAAVGGAYD FTRAAAANLR EKEDWVNNG IGGLFAGATM GLTTGRIPRV LGFAALTGVV LATAEYAGSG LRGVFKRDVD EYERKEFLRK NRRRPIEETL A EIGEGRGI ...String:
MAPIEEEHEH YHPKDAVHLG LKGAAVVGGI GLLFAAVRTS LARKNVGPWA IFTRNGKLAA TFAAVGGAYD FTRAAAANLR EKEDWVNNG IGGLFAGATM GLTTGRIPRV LGFAALTGVV LATAEYAGSG LRGVFKRDVD EYERKEFLRK NRRRPIEETL A EIGEGRGI KPPGYYERRA QRLKEKYGVD INPVCADPDQ A

+
Macromolecule #11: NADH-ubiquinone oxidoreductase chain 4L

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 4L / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 9.837997 KDa
SequenceString:
MNITLILFLI GILGFVLNRK NIILMLISIE IMLLSITFLI LLSSLNMDDI IGQTYAIYII VVAGAESAIG LGILVAFYRL RGSIAIEYK

+
Macromolecule #12: Acyl carrier protein

MacromoleculeName: Acyl carrier protein / type: protein_or_peptide / ID: 12 / Details: ZMP covalently linked to Ser98. / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 15.593649 KDa
SequenceString:
MFRSAVLRSA AAATRTTIRS IPPAAAKKFA VAPVSRVTSF IPKTASWQVI RCYASNEGLQ KVEVYERIKS LLAGFDKVND PNNITETAH FANDLGLDSL DTVEVVMAIE EEFSIEIPDK DADQIHSVDK AIEYILRQPD AH

+
Macromolecule #13: Complex I-B22

MacromoleculeName: Complex I-B22 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 11.636524 KDa
SequenceString:
MSNRQAALSL YRRALKLALD WTVHRNLWRG QALYIRSLFE KNRNVTDPRL QRALLKETEK LLEKWKHPDP YCPPTAPGGS KYERNLPVP NLEPPPPLKF

+
Macromolecule #14: Complex I-ESSS

MacromoleculeName: Complex I-ESSS / type: protein_or_peptide / ID: 14
Details: Sequence renumbered (compared to entry sequence) according to map density and genome sequence.
Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 15.847807 KDa
SequenceString:
MDGGPPTFAF RPTARQAPGK LSSAPVTTRL AAAALSRAST VSSKALTPAR FRFFSTTQRR AGGHGMQYDP PTGWLWGVRP GEKYQNEGW EGPFFYGFWG SLIVFAIAYA YKPDTSIQTW ALEEARRRLE AEGILEDPNP TKKE

+
Macromolecule #15: NADH-ubiquinone oxidoreductase

MacromoleculeName: NADH-ubiquinone oxidoreductase / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 21.517605 KDa
SequenceString:
MATRKPAFNQ HVLLDTTPLP DSIPKVKEIG ASSAPLLSAS FFIGARCKDY NDDYMQCKNE NPGRGEFECL KEGRRVTRCA RSVLKDINT HCLEQFRAHW QCLDNNNQQL WQCRPAEWKL NKCVYENLKL EKVIPDQPKN STPVHLRQRQ IFAHHAIPPW E RPFIPGQP EPQLPAGIEI PEKYKNQS

+
Macromolecule #16: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 14.039227 KDa
SequenceString:
MPQDMPPPGG YEAVQYKRNL PSRGLFRPRP LLAGAAVLML YGWYKLVKGI REQNELAREK MWARIHLIPL LQAEEDRDHV RRYLADQAR EKGLLGENIK VYNSDRYVRP TFAVTPSKPA QE

+
Macromolecule #17: NADH-ubiquinone oxidoreductase-like protein

MacromoleculeName: NADH-ubiquinone oxidoreductase-like protein / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 21.645502 KDa
SequenceString:
MSNTPTQTYQ FPSKTVKTDY PLIDNDPHFT RVIRYARPSD YAHGLAAAAA GPAALWLMER ISPSQVGRGG FAKAMRLAGF IGLAGGFLY FYQRSILRFY GMSENAREVE MDMREMTDRV KAGLPLYGES RLSPAMQGVA ARQSRYSALF FGVMPWFNFV N HNQHGVDT AKYYQQAERE LEAERLAREQ AQQ

+
Macromolecule #18: NADH dehydrogenase (Ubiquinone)-like protein

MacromoleculeName: NADH dehydrogenase (Ubiquinone)-like protein / type: protein_or_peptide / ID: 18
Details: Discrepancy with annotated entry sequence. Probably wrong intron-exon boundaries. Amino acids removed and added according to map density, genome sequence and MS data.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 89.210109 KDa
SequenceString: MLSRRLVRAV APLRSPVLPA ARRLPLIQQR TFLPEAMVGR SKIDEKYPDS DYPTLTDKED PDMNGGYINP PRIKRQFRDP HADWWDKQE RRNFGEPVHE DHDILGMFSP YEYTWITPGK GLFQIGLFIA SFLGLCYVVK LTYPDRVSYP REFEGGLERE L GGAGAVRA ...String:
MLSRRLVRAV APLRSPVLPA ARRLPLIQQR TFLPEAMVGR SKIDEKYPDS DYPTLTDKED PDMNGGYINP PRIKRQFRDP HADWWDKQE RRNFGEPVHE DHDILGMFSP YEYTWITPGK GLFQIGLFIA SFLGLCYVVK LTYPDRVSYP REFEGGLERE L GGAGAVRA FLCLDDEIMW MVSLYCLPAS KLISSPVALQ DKSTSSASAM RYDDWDVILF PTGRDSKIPF KEFKVACHVV PD IELAHLH GAVGSPVMTC FVPSLPPGTP FQVSIHCWRR PEISQFARTY SKNPDLVKFE ARVTLDGRLV ASAILDRDVN GPH LITSTF EFTKTGELER LTFPAFRQEI LRQNHWHPGD DLGRIKVIIS EGFPRDSLTV PIERVKNIVT FSFQHAPLGK IPGI AWPNP GMWRRPTPNP AVSVPTYFPG DGAESHAHSP GKRSLLLKGI RNHGFPSTVI PGSIFHHQSN PAGLLNPPGF KVPHF SASN PSVPNIFSPH DPFAEPTYRD WMSSITNVQA GDFWDGRTTW PINPRNFHKS DTIMADCPSQ GGDPMQISGS SLEDDP LRL KAPQNTPTEG GEGPNPGAQF AHPIPSELTA DLESALSQSL LNQAPTSAIS QRNFPMPHSD GLSRKEGRQV SLGQGTS AQ MPSTSSSMEA RRLSQALFGM NNLPIEASVN NGVSASVTPL FAANQRSVNN PLVATFGSAI LSQGSTNPSG TEQSTDTT A TTTAAAAAAV TDVQVEPPAP TSNAANESVI NLTSGTSSTS TVHANVPTSV SKRPRNFTPA SARVIDEEDE PRRTSPQVQ VGGFAETTSV EESIQ

+
Macromolecule #19: Subunit NDUFC2 of NADH-ubiquinone oxidoreductase (Complex I)

MacromoleculeName: Subunit NDUFC2 of NADH-ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 10.823453 KDa
SequenceString:
MVNRILFWTG FGLAVRFWQL GIEMRPFFNR KSLWAYPLFG GVGASFGYWL QSIDEKQTKM LEERKQAILE KRARRAQRQA EAAATAPSP SAQEA

+
Macromolecule #20: Subunit NDUFB3 of NADH-ubiquinone oxidoreductase (Complex I)

MacromoleculeName: Subunit NDUFB3 of NADH-ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 11.153536 KDa
SequenceString:
MQPTRILRNN GEKPNITGFD MREFLRHTKT PTYDPWERHE AWRYTGRFSR FNRFKGALPG FGIATVAFTA YCVFEHFFLK DDHHGHHGE KEHH

+
Macromolecule #21: Subunit NDUFB10 of NADH-ubiquinone oxidoreductase (Complex I)

MacromoleculeName: Subunit NDUFB10 of NADH-ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 12.514257 KDa
SequenceString:
MPTPESEAFL AKKPQVPPTF DGVDYEDNKR LKQAQDAIIR EQWVQVMMGR LVREELSKCY YREGVNHLEK CGKLRERYLQ LLANAKVKG YLFEQQNYWS KENQQQ

+
Macromolecule #22: Subunit NDUFB2 of NADH-ubiquinone oxidoreductase (Complex I)

MacromoleculeName: Subunit NDUFB2 of NADH-ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 22
Details: No entry (not annotated). Sequence modelled according to map density, genome sequence and MS data.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Molecular weightTheoretical: 5.27815 KDa
SequenceString:
MAGGGQHVSR VHRFLATGLG ASMWFWIFYR AKKDGPVLLG WKHPWD

+
Macromolecule #23: Subunit NDUFA3 of NADH-ubiquinone oxidoreductase (Complex I)

MacromoleculeName: Subunit NDUFA3 of NADH-ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 9.076461 KDa
SequenceString:
MSATTPRFWS TPLKYCRWAA RERPALFFSV VIGALGPVTL ATVPPLRRLI GDVDAAPIPL TYPIPPGPRK QLKGYDDDTE DN

+
Macromolecule #24: Subunit NDUFB6 of NADH-ubiquinone oxidoreductase (Complex I)

MacromoleculeName: Subunit NDUFB6 of NADH-ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 10.857294 KDa
SequenceString:
MGGGPKIPYP KHVWSPAGGW YAQPANWKQN TAIFGLVIFG ITAMVWKYSA EHEVRHKMPE PDRFYPSRYW VKQIKDYERA QKEKQQNNT EASS

+
Macromolecule #25: Subunit NDUFB4 of NADH-ubiquinone oxidoreductase (Complex I)

MacromoleculeName: Subunit NDUFB4 of NADH-ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 8.760336 KDa
SequenceString:
MAGLQHYKIA MDPALVRLGS MISNRYKYFR WTKRTALVSF MYVVVVPSTI GYLAYKTDGL WDLRAKRRGD LISER

+
Macromolecule #26: Subunit NDUFB5 of NADH-ubiquinone oxidoreductase (Complex I)

MacromoleculeName: Subunit NDUFB5 of NADH-ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 26
Details: Discrepancy with annotated entry sequence. Probably wrong intron-exon boundaries. Amino acids added and removed according to map density and genome sequence.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 20.824342 KDa
SequenceString:
MLALRQRAAL LARRVRPTVV VPRNARTYAS SHDHDHHDHH HDHGHNVEEP LGAAFYIAVG GIASSFVIYN ISRPGPNGEP SSLHKWFSK ISDYKDEWET RNTLMAAALE QAAHDKHLLL TAERSRHIEL KYPEVFSHGS PFNVPAGFYP NLDHVIEHYR K QHLEEEER KAKKLAAAAA AASEAR

+
Macromolecule #27: 1,2-Distearoyl-sn-glycerophosphoethanolamine

MacromoleculeName: 1,2-Distearoyl-sn-glycerophosphoethanolamine / type: ligand / ID: 27 / Number of copies: 17 / Formula: 3PE
Molecular weightTheoretical: 748.065 Da
Chemical component information

ChemComp-3PE:
1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipid*YM / Phosphatidylethanolamine

+
Macromolecule #28: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE

MacromoleculeName: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / type: ligand / ID: 28 / Number of copies: 8 / Formula: PC1
Molecular weightTheoretical: 790.145 Da
Chemical component information

ChemComp-PC1:
1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM / Phosphatidylcholine

+
Macromolecule #29: CARDIOLIPIN

MacromoleculeName: CARDIOLIPIN / type: ligand / ID: 29 / Number of copies: 5 / Formula: CDL
Molecular weightTheoretical: 1.464043 KDa
Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM / Cardiolipin

+
Macromolecule #30: Lauryl Maltose Neopentyl Glycol

MacromoleculeName: Lauryl Maltose Neopentyl Glycol / type: ligand / ID: 30 / Number of copies: 3 / Formula: LMN
Molecular weightTheoretical: 1.005188 KDa
Chemical component information

ChemComp-AV0:
Lauryl Maltose Neopentyl Glycol

+
Macromolecule #31: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-b...

MacromoleculeName: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate
type: ligand / ID: 31 / Number of copies: 1 / Formula: ZMP
Molecular weightTheoretical: 568.704 Da
Chemical component information

ChemComp-ZMP:
S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate

+
Macromolecule #32: water

MacromoleculeName: water / type: ligand / ID: 32 / Number of copies: 1111 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER / Water

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

Concentration1.5 mg/mL
BufferpH: 7.4
Component:
ConcentrationFormulaName
20.0 mMHepes/NaOH4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (pH adjusted with sodium hydroxide)
100.0 mMNaClSodium chloridesodium chloride
0.0015 % [w/v]LMNGlauryl maltose neopentyl glycol
GridModel: C-flat-1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 45 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 105000
Specialist opticsEnergy filter - Name: GIF Bioquantum / Energy filter - Slit width: 30 eV
Sample stageCooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 1 / Number real images: 6503 / Average exposure time: 2.11 sec. / Average electron dose: 45.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Particle selectionNumber selected: 1087651
Initial angle assignmentType: NOT APPLICABLE / Software - Name: cryoSPARC (ver. 3.0.1)
Final angle assignmentType: NOT APPLICABLE
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.47 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 3.0.1) / Details: Local refinement on membrane arm of complex I / Number images used: 153568
FSC plot (resolution estimation)

-
Atomic model buiding 1

Initial model(PDB ID:

6rfr

,

6rfq

)
Output model

PDB-7zmh:
CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (state 1) - membrane arm

+
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