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- EMDB-14794: CryoEM structure of mitochondrial complex I from Chaetomium therm... -

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Basic information

Entry
Database: EMDB / ID: EMD-14794
TitleCryoEM structure of mitochondrial complex I from Chaetomium thermophilum (state 2)
Map dataDensity modified map (Terwilliger et al., 2020)
Sample
  • Complex: Mitochondrial NADH:ubiquinone oxidoreductase in LMNG
    • Protein or peptide: x 42 types
  • Ligand: x 11 types
Function / homology
Function and homology information


single-stranded DNA helicase activity / respiratory chain complex I / oxidoreductase activity, acting on NAD(P)H / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / electron transport chain / NADH dehydrogenase (ubiquinone) activity / quinone binding ...single-stranded DNA helicase activity / respiratory chain complex I / oxidoreductase activity, acting on NAD(P)H / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / electron transport chain / NADH dehydrogenase (ubiquinone) activity / quinone binding / ATP synthesis coupled electron transport / respirasome / respiratory electron transport chain / mitochondrial membrane / mitochondrial intermembrane space / 2 iron, 2 sulfur cluster binding / fatty acid biosynthetic process / NAD binding / FMN binding / 4 iron, 4 sulfur cluster binding / DNA helicase / mitochondrial inner membrane / DNA replication / oxidoreductase activity / hydrolase activity / DNA repair / mitochondrion / DNA binding / ATP binding / membrane / metal ion binding / nucleus
Similarity search - Function
Oxidoreductase-like, N-terminal / Oxidoreductase-like domain-containing protein 1 / Oxidoreductase-like protein, N-terminal / NADH-ubiquinone oxidoreductase 17.8kDa subunit / : / Dna2 Rift barrel domain / DNA replication factor Dna2, N-terminal / DNA replication ATP-dependent helicase/nuclease Dna2 / DNA replication factor Dna2 / NADH dehydrogenase [ubiquinone] (complex I), 21kDa subunit, fungi ...Oxidoreductase-like, N-terminal / Oxidoreductase-like domain-containing protein 1 / Oxidoreductase-like protein, N-terminal / NADH-ubiquinone oxidoreductase 17.8kDa subunit / : / Dna2 Rift barrel domain / DNA replication factor Dna2, N-terminal / DNA replication ATP-dependent helicase/nuclease Dna2 / DNA replication factor Dna2 / NADH dehydrogenase [ubiquinone] (complex I), 21kDa subunit, fungi / Dna2/Cas4, domain of unknown function DUF83 / Domain of unknown function DUF83 / NADH-ubiquinone oxidoreductase, 21kDa subunit, C-terminal, fungi / NADH-ubiquinone oxidoreductase 9.5kDa subunit / C-terminal of NADH-ubiquinone oxidoreductase 21 kDa subunit / NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit / : / DNA2/NAM7 helicase, helicase domain / DNA2/NAM7-like helicase / AAA domain / PD-(D/E)XK endonuclease-like domain superfamily / NAD(P)H-quinone oxidoreductase, subunit N/subunit 2 / Complex1_LYR-like / NADH:ubiquinone oxidoreductase, ESSS subunit / ESSS subunit of NADH:ubiquinone oxidoreductase (complex I) / Soluble ligand binding domain / SLBB domain / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / Zinc finger, CHCC-type / Zinc-finger domain / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 / GRIM-19 / NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8) / GRIM-19 protein / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase subunit 5, C-terminal / NADH dehydrogenase subunit 5 C-terminus / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family / NDUFA6, LYR domain / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7 / NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7) / NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily / NADH dehydrogenase ubiquinone Fe-S protein 4 / NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / NDUFB9, LYR domain / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH ubiquinone oxidoreductase subunit NDUFA12 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5 / ETC complex I subunit conserved region / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / NADH-quinone oxidoreductase, chain G, C-terminal / NADH-ubiquinone oxidoreductase subunit G, C-terminal / Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. / NADH-ubiquinone oxidoreductase, 20 Kd subunit / NADH-quinone oxidoreductase, chain I / NAD(P)H-quinone oxidoreductase subunit D/H / NADH:ubiquinone oxidoreductase, 49kDa subunit, conserved site / Respiratory chain NADH dehydrogenase 49 Kd subunit signature. / NADH-quinone oxidoreductase, subunit D / Respiratory-chain NADH dehydrogenase, 49 Kd subunit / NADH:ubiquinone oxidoreductase, subunit G / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2. / NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ / NADH dehydrogenase, subunit C / NADH ubiquinone oxidoreductase, F subunit / Complex 1 LYR protein domain / NADH-plastoquinone oxidoreductase, chain 5 subgroup / NADH:ubiquinone oxidoreductase, 30kDa subunit, conserved site / Respiratory chain NADH dehydrogenase 30 Kd subunit signature. / NADH-quinone oxidoreductase, chain M/4 / Complex 1 protein (LYR family) / 2Fe-2S iron-sulfur cluster binding domain / NADH-ubiquinone oxidoreductase chain 4L/K / NADH:ubiquinone/plastoquinone oxidoreductase, chain 6 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6 / NADH:ubiquinone oxidoreductase, 75kDa subunit, conserved site / NADH:ubiquinone oxidoreductase, 30kDa subunit / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminal / NADH-quinone oxidoreductase, chain 5-like / NADH:ubiquinone oxidoreductase, 30kDa subunit superfamily / Respiratory-chain NADH dehydrogenase, 30 Kd subunit / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region / Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 1. / NADH-ubiquinone oxidoreductase chain 4L/Mnh complex subunit C1-like / NADH-ubiquinone/plastoquinone oxidoreductase chain 4L / NADH:ubiquinone/plastoquinone oxidoreductase, chain 3 / NADH:ubiquinone oxidoreductase, subunit 3 superfamily / NADH-ubiquinone/plastoquinone oxidoreductase, chain 3 / Respiratory-chain NADH dehydrogenase 24 Kd subunit signature. / NADH:ubiquinone oxidoreductase, subunit 1, conserved site / Respiratory-chain NADH dehydrogenase subunit 1 signature 1.
Similarity search - Domain/homology
NADH dehydrogenase (Ubiquinone)-like protein / NADH-ubiquinone oxidoreductase-like protein / NADH-ubiquinone oxidoreductase 9.5 kDa subunit / NADH-ubiquinone oxidoreductase-like protein / NADH-ubiquinone oxidoreductase / NADH-ubiquinone oxidoreductase-like protein / NADH dehydrogenase-like protein / NADH-ubiquinone oxidoreductase-like protein / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / NADH-ubiquinone oxidoreductase B14 subunit-like protein ...NADH dehydrogenase (Ubiquinone)-like protein / NADH-ubiquinone oxidoreductase-like protein / NADH-ubiquinone oxidoreductase 9.5 kDa subunit / NADH-ubiquinone oxidoreductase-like protein / NADH-ubiquinone oxidoreductase / NADH-ubiquinone oxidoreductase-like protein / NADH dehydrogenase-like protein / NADH-ubiquinone oxidoreductase-like protein / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / NADH-ubiquinone oxidoreductase B14 subunit-like protein / Uncharacterized protein / NADH-ubiquinone oxidoreductase B15 subunit / Uncharacterized protein / NADH-ubiquinone oxidoreductase-like protein / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit / Uncharacterized protein / NADH-ubiquinone oxidoreductase-like protein / NADH-ubiquinone oxidoreductase 30.4 kDa subunit-like protein / Oxidoreductase-like domain-containing protein / NADH-ubiquinone oxidoreductase-like protein / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / DNA replication ATP-dependent helicase/nuclease DNA2 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH dehydrogenase (Ubiquinone)-like protein / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / Oxidoreductase-like protein / Acyl carrier protein / NADH-ubiquinone oxidoreductase 49 kDa subunit-like protein / NADH-ubiquinone oxidoreductase-like protein / NADH-ubiquinone oxidoreductase-like protein / NADH-ubiquinone oxidoreductase-like protein / Uncharacterized protein / NADH-ubiquinone oxidoreductase chain 6 / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 4L / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 1 / NADH-ubiquinone oxidoreductase chain 4
Similarity search - Component
Biological speciesChaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.75 Å
AuthorsLaube E / Kuehlbrandt W
Funding support Germany, 1 items
OrganizationGrant numberCountry
Max Planck Society Germany
CitationJournal: Sci Adv / Year: 2022
Title: Conformational changes in mitochondrial complex I of the thermophilic eukaryote .
Authors: Eike Laube / Jakob Meier-Credo / Julian D Langer / Werner Kühlbrandt /
Abstract: Mitochondrial complex I is a redox-driven proton pump that generates proton-motive force across the inner mitochondrial membrane, powering oxidative phosphorylation and ATP synthesis in eukaryotes. ...Mitochondrial complex I is a redox-driven proton pump that generates proton-motive force across the inner mitochondrial membrane, powering oxidative phosphorylation and ATP synthesis in eukaryotes. We report the structure of complex I from the thermophilic fungus , determined by cryoEM up to 2.4-Å resolution. We show that the complex undergoes a transition between two conformations, which we refer to as state 1 and state 2. The conformational switch is manifest in a twisting movement of the peripheral arm relative to the membrane arm, but most notably in substantial rearrangements of the Q-binding cavity and the E-channel, resulting in a continuous aqueous passage from the E-channel to subunit ND5 at the far end of the membrane arm. The conformational changes in the complex interior resemble those reported for mammalian complex I, suggesting a highly conserved, universal mechanism of coupling electron transport to proton pumping.
History
DepositionApr 19, 2022-
Header (metadata) releaseNov 30, 2022-
Map releaseNov 30, 2022-
UpdateDec 7, 2022-
Current statusDec 7, 2022Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_14794.png

Downloads & links

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Map

FileDownload / File: emd_14794.map.gz / Format: CCP4 / Size: 73.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationDensity modified map (Terwilliger et al., 2020)
Projections & slices

Image control

Size
Brightness
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Others
AxesX (Sec.)Y (Row.)Z (Col.)
0.84 Å/pix.
x 190 pix.
= 159.03 Å		0.84 Å/pix.
x 265 pix.
= 221.805 Å		0.84 Å/pix.
x 382 pix.
= 319.734 Å

Surface

Projections

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Slices (1/2)

Slices (2/3)

Images are generated by Spider.

generated in cubic-lattice coordinate

Voxel sizeX=Y=Z: 0.837 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.12
Minimum - Maximum-1.3737229 - 2.67497
Average (Standard dev.)8.9715697e-13 (±0.10974552)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin15197205
Dimensions265382190
Spacing190265382
CellA: 159.03 Å / B: 221.80501 Å / C: 319.734 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: Unsharpened/unmodified map

Fileemd_14794_additional_1.map
AnnotationUnsharpened/unmodified map
Projections & Slices
AxesZYX

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Half map: EM half map A

Fileemd_14794_half_map_1.map
AnnotationEM_half_map_A
Projections & Slices
AxesZYX

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Density Histograms

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Histogram (log scale)

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Half map: EM half map B

Fileemd_14794_half_map_2.map
AnnotationEM_half_map_B
Projections & Slices
AxesZYX

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Slices (1/2)
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Sample components

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Entire : Mitochondrial NADH:ubiquinone oxidoreductase in LMNG

EntireName: Mitochondrial NADH:ubiquinone oxidoreductase in LMNG
Components
  • Complex: Mitochondrial NADH:ubiquinone oxidoreductase in LMNG
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 1
    • Protein or peptide: NADH dehydrogenase subunit 2
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 3
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 5
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 6
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
    • Protein or peptide: Subunit NDUFS5 of NADH-ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NADH-ubiquinone oxidoreductase-like protein
    • Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
    • Protein or peptide: NADH-ubiquinone oxidoreductase 49 kDa subunit-like protein
    • Protein or peptide: Subunit NDUFA1 of NADH-ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NADH dehydrogenase (Ubiquinone)-like protein
    • Protein or peptide: NADH-ubiquinone oxidoreductase-like protein
    • Protein or peptide: NADH-ubiquinone oxidoreductase 30.4 kDa subunit-like protein
    • Protein or peptide: Subunit NDUFV2 of NADH-ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: Oxidoreductase-like protein
    • Protein or peptide: NADH-ubiquinone oxidoreductase-like protein
    • Protein or peptide: NADH-ubiquinone oxidoreductase-like protein
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4L
    • Protein or peptide: NADH-ubiquinone oxidoreductase-like protein
    • Protein or peptide: Acyl carrier protein
    • Protein or peptide: NADH-ubiquinone oxidoreductase B14 subunit-like protein
    • Protein or peptide: Complex I-B22
    • Protein or peptide: Complex I-ESSS
    • Protein or peptide: NADH-ubiquinone oxidoreductase
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
    • Protein or peptide: NADH-ubiquinone oxidoreductase-like protein
    • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
    • Protein or peptide: NADH-ubiquinone oxidoreductase-like protein
    • Protein or peptide: NADH dehydrogenase (Ubiquinone)-like protein
    • Protein or peptide: Subunit NDUFC2 of NADH-ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: Subunit NDUFB3 of NADH-ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: Subunit NDUFB10 of NADH-ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: Subunit NDUFB2 of NADH-ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NADH dehydrogenase-like protein
    • Protein or peptide: Subunit NDUFA3 of NADH-ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit
    • Protein or peptide: Subunit NDUFB6 of NADH-ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: Subunit NDUFB4 of NADH-ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: Subunit NDUFB5 of NADH-ubiquinone oxidoreductase (Complex I)
    • Protein or peptide: Oxidoreductase-like domain-containing protein
  • Ligand: 1,2-Distearoyl-sn-glycerophosphoethanolamine
  • Ligand: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
  • Ligand: CARDIOLIPIN
  • Ligand: Lauryl Maltose Neopentyl Glycol
  • Ligand: FE2/S2 (INORGANIC) CLUSTER
  • Ligand: IRON/SULFUR CLUSTERIron–sulfur cluster
  • Ligand: FLAVIN MONONUCLEOTIDE
  • Ligand: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
  • Ligand: ZINC ION
  • Ligand: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate
  • Ligand: water

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Supramolecule #1: Mitochondrial NADH:ubiquinone oxidoreductase in LMNG

SupramoleculeName: Mitochondrial NADH:ubiquinone oxidoreductase in LMNG / type: complex / Chimera: Yes / ID: 1 / Parent: 0 / Macromolecule list: #1-#42
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Molecular weightTheoretical: 970 KDa

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Macromolecule #1: NADH-ubiquinone oxidoreductase chain 1

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 41.716406 KDa
SequenceString: MSYSQTINSL VEVVLVLVPS LVGIAYVTVG ERKTMGSMQR RLGPNAVGIY GLLQAFADAL KLLLKEYVGP TQANLVLFFL GPVITLIFS LLGYAVIPYG PGLAVNDLST GILYMLAVSS LATYGILLAG WSANSKYAFL GSLRSTAQLI SYELVLSSSI L LVIMLSGS ...String:
MSYSQTINSL VEVVLVLVPS LVGIAYVTVG ERKTMGSMQR RLGPNAVGIY GLLQAFADAL KLLLKEYVGP TQANLVLFFL GPVITLIFS LLGYAVIPYG PGLAVNDLST GILYMLAVSS LATYGILLAG WSANSKYAFL GSLRSTAQLI SYELVLSSSI L LVIMLSGS LSLTVIVESQ RAIWYILPLL PVFIIFFIGS VAETNRAPFD LAEAESELVS GFMTEHAAVI FVFFFLAEYG SI VLMCILT SILFLGGYLL ISLLDIIYNN LLSWIVIGKY IIFIFPFWGP VFIDLGLYEI ISYLYNAPTV EGSFYGLSLG VKT SILIFV FIWTRASFPR IRFDQLMSFC WTVLLPILFA LIVLVPCILY SFNIFPVNIS LL

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Macromolecule #2: NADH dehydrogenase subunit 2

MacromoleculeName: NADH dehydrogenase subunit 2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 64.12918 KDa
SequenceString: MIMISILSLL LSTSVTLRRD MSILFNRISI IALAYCILHD TMSLSFISKG IGLHGGLLHI TNLTQIFHIF IFIISILILQ LTSFYPRKV WIPEYSSLKD IFFNKILYYR TKIINKMGEH MKIIEYPLIL LFVISGAVFL ISTNDLVSIF LSIELQSYGL Y LLSTIYRN ...String:
MIMISILSLL LSTSVTLRRD MSILFNRISI IALAYCILHD TMSLSFISKG IGLHGGLLHI TNLTQIFHIF IFIISILILQ LTSFYPRKV WIPEYSSLKD IFFNKILYYR TKIINKMGEH MKIIEYPLIL LFVISGAVFL ISTNDLVSIF LSIELQSYGL Y LLSTIYRN SELSTTGGLI YFLLGGLSSC FILLGTSLLY VNSGTTSLDG LYILNSISDV NPVVAGVGED GGLTSWYKPY YL NFSLLIF SIGFLFKVSA APFHFWSPDV YDAIPTIVTT FVAIIAKISI FIFLLELVYY TNSNANSYLS EFSWTYALLI SSL LSLIIG TVVGLTQFRI KRLLAYSTIS HVGFILLALS VSSIESTQAF IFYLIQYSIS NLNAFFILIT IGFSLYGYVT NNKE YKSLL DKNNSPIQLI SQLKGYFYIN PLLSLSLAIT IFSFVGVPPL VGFFAKQMVL SAALDNGYIF LSLIAIITSV IGAVY YLNV IKEIFFYSPE HEVNPVLNES DSNFSLRILN EKNVLIRSVL LKGRNIFISS PFSITISIIT NVILLFIFMN KEWLSM GTI LVQILFSA

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Macromolecule #3: NADH-ubiquinone oxidoreductase chain 3

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 16.33003 KDa
SequenceString:
MSAMSIYIIF VSIIAILFLA IDLIFAPHNP YKEKLSAFEC GFHSFSQSRS PFNISFFIYG LVFLLLDLEI LLLYPFAVSE YVNSAYGLA AALIFIGIIT IGFVYELGHD ALKVHSRQNI STKDLKSSVV ISYLGNINND SVNLHIK

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Macromolecule #4: NADH-ubiquinone oxidoreductase chain 4

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 4 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 60.810309 KDa
SequenceString: MSLLYVLLII PIIGIFLIST IDSFYPVTNT NIVLNKKFFR GFNDARQPIK YLYFSEGESF NIENKEDSFF NVSYYKKIAL ITTILNLIV SLIIYILFDF SNNQFQFIQE NLDLSFYDIY LGVDGVSIYF VLLTTIIMPI ALVSNWNSIT NNIKSYLIIM L LLETLLLA ...String:
MSLLYVLLII PIIGIFLIST IDSFYPVTNT NIVLNKKFFR GFNDARQPIK YLYFSEGESF NIENKEDSFF NVSYYKKIAL ITTILNLIV SLIIYILFDF SNNQFQFIQE NLDLSFYDIY LGVDGVSIYF VLLTTIIMPI ALVSNWNSIT NNIKSYLIIM L LLETLLLA VFLVLDVLLF YIFFESILPP LFILIGLFGS SNKVRASFYI FLYTLLGSLF LLLSILTMSS IVGTTYFDVL LK SSFEYTT QLFLFFGIFI AFAVKTPVWG LNSWLLRAHV ESPLGGSIVL AAIVLKLSLY GVFRLILPIL PQASLNLTYI VYA IGAITV LYASFSTLRT VDVKELIAYS SVAHAAIYLM GVFSNTIQGL EGAILLGLAH GFVSSGLFIC AGGILYDRTG TRLI YFFRG LTQIMPLFSL FFFILCLGNA GTPLTLNFVG EFMSLYGTLE RLPIAGMLAS TSIIFSAAYS IYMYNRIAFG GSVSL YFID CFRDLTKREF FILFTLVSFT VILGIYPSFV LDGLHYNISS VVYGIEPNAS YLTQGGNL

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Macromolecule #5: NADH-ubiquinone oxidoreductase chain 5

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 5 / type: protein_or_peptide / ID: 5
Details: Discrepancy with annotated entry sequence. Probably wrong intron-exon boundaries. Amino acids added according to map density, genome sequence and MS data.
Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 75.872031 KDa
SequenceString: MYLSIIILPL LGSVVSGFFG RKVGVSGAQL ITCSSVIITT ILSIIAFFEV GFNNIPVTIN IFRWIDSEWF IINWGFQYDS LTVSMLIPV LIISSLVHIY SISYMSSDPH NQRFFSYLSL FTFMMIILVT ANNYLLMFVG WEGVGVCSYL LVSFWFTRIA A NQSSISAF ...String:
MYLSIIILPL LGSVVSGFFG RKVGVSGAQL ITCSSVIITT ILSIIAFFEV GFNNIPVTIN IFRWIDSEWF IINWGFQYDS LTVSMLIPV LIISSLVHIY SISYMSSDPH NQRFFSYLSL FTFMMIILVT ANNYLLMFVG WEGVGVCSYL LVSFWFTRIA A NQSSISAF LTNRVGDCFL TVGMFAILWS LGNLDYATVF SLAPYINSNV VIIIGICLLI GAMAKSSQVG LHVWLPMAME GP TPVSALI HAATMVTAGV YLLMRSSPLI EYSSTVLLLC LWLGAITTVF SSLIGLFQQD IKKVIAYSTM SQLGMMVLSI GLS SYNIAL FHLVNHAFYK ALLFLGAGSV IHAVADNQDF RKFGGLISYL PLTYSVMLIA SLSLVAFPFM TGFYSKDFIL ESAY GQFSF SGVAVYIIAT IGAIFTTLYS VKVLYLTFLS NPNGPRTYYR LAIDNFFSAQ AIKSYKPAHE GDFFLTLPLV ILALF SIFF GFITKDIFIG LGSNFFVDNS LFIHPIHEIM IDTEFAVPVL FKLLPFIFTI SFSVIALTLS ELLSELVIYF KFSRFG YNI FGFFNQRFLI EFFYNKYITN LILNLGGQIT KILDKGSIEL FGPYGLERGL VKLSKNISSL STSHVTTYAL YILVGFI LY LIYNNLLLDY SYLLLIIILL LLLMMIGESN SEDVTLH

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Macromolecule #6: NADH-ubiquinone oxidoreductase chain 6

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 6 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 24.819291 KDa
SequenceString: MNSQISLLLL KEIYTNGSTH IMLDILSVLA VISGICVIIS KNPIVSVLHL IGLFAYVSFY LILIGLNFVG LSYLIVYIGA VSILFLFIL MLINIRTSEL QSNTSNSIPL TILVGIIISS FLFKMLPYGV IISNQFNSSN LNENLYTIQI VGGEDNNINN I NTDKNDLF ...String:
MNSQISLLLL KEIYTNGSTH IMLDILSVLA VISGICVIIS KNPIVSVLHL IGLFAYVSFY LILIGLNFVG LSYLIVYIGA VSILFLFIL MLINIRTSEL QSNTSNSIPL TILVGIIISS FLFKMLPYGV IISNQFNSSN LNENLYTIQI VGGEDNNINN I NTDKNDLF FITSKIWDGA LAENNHISSI GNIMYTNYNV WLILASFILL LAMVGAIVIT IKPRKI

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Macromolecule #7: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 10.31275 KDa
SequenceString:
MATDAAETSR RATREEMRDA KVPLAYRDSC AHLLIPLNRC RYETYYLPWK CEDERHSYEK CQYLEFKKRV QKMEELREAK GGARSN

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Macromolecule #8: Subunit NDUFS5 of NADH-ubiquinone oxidoreductase (Complex I)

MacromoleculeName: Subunit NDUFS5 of NADH-ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 8
Details: Discrepancy with annotated entry sequence. Probably wrong intron-exon boundaries. One additional amino acid modelled according to map density and genome sequence.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 87.142328 KDa
SequenceString: MASGYGLNGG PSRCFPFWQE LLACYVTNSS EDNPDGKNKC IPVMEDYYEC LHHRKEAARV RALQAAYREA EAKKLQENPP TAGQIRNLG LLNKEEDTKK VHCATATQQW MKMCFALSAK MPCLRGIEVF LTTRPDNEKI PEYPHPEGTS ARVICGQHLI R SPSLASNL ...String:
MASGYGLNGG PSRCFPFWQE LLACYVTNSS EDNPDGKNKC IPVMEDYYEC LHHRKEAARV RALQAAYREA EAKKLQENPP TAGQIRNLG LLNKEEDTKK VHCATATQQW MKMCFALSAK MPCLRGIEVF LTTRPDNEKI PEYPHPEGTS ARVICGQHLI R SPSLASNL SNSAITPPSS FSPRKANPLV SVYLPSVPGT PFTINYKISQ VPPEPCKYLF FRLYINARPM VSWGIDPHSR PY GKVIKSL WLPSDDRYRG LVGFEKRSFV FLPGEEFKSV AEDGGLIEVQ VFRAKDRRAR TPKLETFRFR DNYGIAAPSI GLL DKPQNA FFYDWLLIDP KDEPFAKFRM HYRSWRNLKS LNLIPSSEWE LLLAVSPKAL RTAASTGKIE KPTSPAFSDS DSDD SLCSA TDSDECVFDD HSKKTKSNRS KESPFAFLNS PPERFRAMAP SSEKLPQPSK LLRDSQRAPY QSRPLPELPV EAGVN STGL NPPSVKVAAD LRRKPSATSM ESNAVSITPS LLRCMEEGTL DLEKAEVGIA KLVKVAASGS EPSSSSSSAT QLTVSA VRE VELRPPQPER KGGLPMDYSF SDYEKSSQSS FGDDERMSNI SDMEEKPFPA PPTCYLPTTG SGLERELAMF DSPSPSP VP YSAMEPPVTP SPSSTPYKTK LGRKPLLFSR RLGLFSPRKS LPSDFLLGQA KKLVVEEETT NSNVSLAFGE LTVTDMRA E SPSPAPKGKP LRRFSTIRVE EISIKEKRPL FNSLRRIASA SPRKLAGRVL SMDLGKKGGE EKEG

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Macromolecule #9: NADH-ubiquinone oxidoreductase-like protein

MacromoleculeName: NADH-ubiquinone oxidoreductase-like protein / type: protein_or_peptide / ID: 9
Details: Discrepancy with annotated entry sequence. Probably wrong intron-exon boundaries. Amino acids removed and added to sequence according to map density, genome sequence and MS data.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 82.096516 KDa
SequenceString: MLRQTLARSA WRTGKHPAAA ARTAGAATRA FSASAQRPAE VELTIDGKKV SIEAGSALIQ ACEKAGVVVP RYCYHEKLAI AGNCRMCLV EVERSPKPVA SCAWPVQAGM VVKTNSPLTH KAREGVMEFL LANHPLDCPI CDQGGECDLQ DQSMRYGADR G RFHEIGGK ...String:
MLRQTLARSA WRTGKHPAAA ARTAGAATRA FSASAQRPAE VELTIDGKKV SIEAGSALIQ ACEKAGVVVP RYCYHEKLAI AGNCRMCLV EVERSPKPVA SCAWPVQAGM VVKTNSPLTH KAREGVMEFL LANHPLDCPI CDQGGECDLQ DQSMRYGADR G RFHEIGGK RAVEDKNIGP LIKTSMNRCI HCTRCVRFMN DIAGAPELGS TGRGNDLQIG TYLEKNLDSE LSGNVIDLCP VG ALTSKPY AFRARPWELK RTESIDVLDG LGSNIRVDSR GLEVMRILPR LNDDVNEEWI NDKTRFACDG LKTQRLTMPL VRR DGKFEP ATWEQALTEI AHAYQTLAPK ENEFKVIAGQ LVEVESLVAM KDLANRLGSE NLALDFPGGS QPLAHGVDIR SNYL FNSKI WGIEEADAIL LVGTNPRHEA AVLNARIRKQ WLRSDLEIAA VGQPWESTFD YEHLGTDLAA LKNALSGPFG EKLKK AKRP MIIVGSGVTE HPDAKAFYET VWSFVEKNAS NFLTEEWCGY NVLQRAASRA GAFEVGFVVP SPEVAATKPK FVWLLG ADE FDPADVPKDA FIVYQGHHGD RGAEIADIVL PGAAYTEKAG TYVNTEGRVQ MTRAATGLPG AARTDWKIIR AVSEFLG VP LPYDDVAQLR DRMAEISPAL AAYDVVEPVA LRHLSKVQLV DQNKGAKVTG EPLKKVVENF YFTDVISRSS PTMARCSA A KETGDPRTNF MAPGMEEDRP MGQIAYGA

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Macromolecule #10: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

MacromoleculeName: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 55.45309 KDa
SequenceString: MLSRTAAPAK ASAKTISSAV SSAAAAAATT SAASTPQSRA YATVQDPNYA TVRTYGGLKD QDRIFQNLYG RYPPDLKHAK KMGDWYKTK EILLKGHDWI IGEIKASGLR GRGGAGFPSG LKWSFMNFKD WDKDNKPRYL VVNADEGEPG TCKDREIMRK D PHKLVEGC ...String:
MLSRTAAPAK ASAKTISSAV SSAAAAAATT SAASTPQSRA YATVQDPNYA TVRTYGGLKD QDRIFQNLYG RYPPDLKHAK KMGDWYKTK EILLKGHDWI IGEIKASGLR GRGGAGFPSG LKWSFMNFKD WDKDNKPRYL VVNADEGEPG TCKDREIMRK D PHKLVEGC LVAGRAMNAT AAYIYIRGEF YQEAAILQNA INEAYAEGLI GKNACGSGYD FDVYIHRGAG AYVCGEETSL IE SLEGKPG KPRLKPPFPA AVGLFGCPST VANVETVSVA PTICRRGGAW FASFGRERNH GTKLFCISGH VNNPCTVEEE MSI PMRELI EKHCGGVRGG WDNLLAVIPG GSSTPILPKH ICDDQLMDFD ALKDSQSGLG TAAVIVMDKS TDVVRAISRL SHFY RHESC GQCTPCREGS KWTDQIMKRF EKGMGRPREI DMLQELTKQV EGHTICALGE AFAWPIQGLI RHFRPELEAR MKKFA EETG GQALAGGWTH DSRQKGRLVS PGM

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Macromolecule #11: NADH-ubiquinone oxidoreductase 49 kDa subunit-like protein

MacromoleculeName: NADH-ubiquinone oxidoreductase 49 kDa subunit-like protein
type: protein_or_peptide / ID: 11
Details: Discrepancy with annotated entry sequence. Probably wrong intron-exon boundaries. Amino acids removed according to map density, genome sequence and MS data.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 55.910316 KDa
SequenceString: MSASLYRLAG RSARRLATSA ASRSRPLTSS SSFQLSSTAS TPLSVRAFSA SALRRYAEPQ DGFQGTRLIP TGADFQSPPD PFIDEGARD NAANYGVQKS RSGVDEDSVE GRKVRHYTVN FGPQHPAAHG VLRLILELNG EEIVRADPHV GLLH(2MR)GT EK LCEYKTYMQA ...String:
MSASLYRLAG RSARRLATSA ASRSRPLTSS SSFQLSSTAS TPLSVRAFSA SALRRYAEPQ DGFQGTRLIP TGADFQSPPD PFIDEGARD NAANYGVQKS RSGVDEDSVE GRKVRHYTVN FGPQHPAAHG VLRLILELNG EEIVRADPHV GLLH(2MR)GT EK LCEYKTYMQA LPYFDRLDYV SMMTNEQVFS LAVEKLLNIE IPPRAKFIRT MFGEITRILN HLMSVLSHAM DVGALTPF L WGFEEREKLM EFYERVSGAR LHAAYVRPGG VHQDIPVGLL DDIYQWATQF GDRIDETEEM LTDNRIWINR LKGVGVVSA ADALNLSFTG VMLRGSGVPW DVRKSSPYDA YDQVEFDVPV GINGDCYDRY LCRMEEFRQS LRIIHQCLNK MPAGPVRYED YKITPPPRA AMKENMEALI HHFLLFTKGY AVPPGDTYTA IEAPKGEMGV YVVSDGSERP YRVHIRAPGF AHLSGFDHIT R GHLLADAV AVIGTMDLVF GEVDR

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Macromolecule #12: Subunit NDUFA1 of NADH-ubiquinone oxidoreductase (Complex I)

MacromoleculeName: Subunit NDUFA1 of NADH-ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 12
Details: No entry (not annotated). Sequence modelled according to map density, genome sequence and MS data. Last five amino acids unknown and modelled as Poly-Ala
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 9.833353 KDa
SequenceString:
MPVPFETLIP YGIIIAMFGV TGAGMAKVRH MFNGDKRHRW SVDQWDKQQM ERDRRLTGHL RGQTDNPIAP PGFEFNNPWK V(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #13: NADH dehydrogenase (Ubiquinone)-like protein

MacromoleculeName: NADH dehydrogenase (Ubiquinone)-like protein / type: protein_or_peptide / ID: 13
Details: Discrepancy with annotated entry sequence. Probably wrong intron-exon boundaries. Amino acids added according to map density, genome sequence and MS data.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 43.800855 KDa
SequenceString: MASLPTAVRS AAPKVARRAL QQRRYISDIH ITRTGKPLIR IQGGRSSLGE HTATVFGATG QLGRYIVNRL ARQGCTVIVP YREEMAKRH LKVSGDLGRV IFMEFDLRNT QSIEESVRHS DVVYNLIGRD YPTKNFSLAD VHIEGTERIV EAVAKYDVDR F IQVSTYNA ...String:
MASLPTAVRS AAPKVARRAL QQRRYISDIH ITRTGKPLIR IQGGRSSLGE HTATVFGATG QLGRYIVNRL ARQGCTVIVP YREEMAKRH LKVSGDLGRV IFMEFDLRNT QSIEESVRHS DVVYNLIGRD YPTKNFSLAD VHIEGTERIV EAVAKYDVDR F IQVSTYNA NPDSTCEFFR TKGIAEKVAR HVFPETTIVR PAPMFGFEDR LLLKLASVTN LFTSNHMREK FWPVHVNEVG EA LERMLYD DSTAGQTFEL YGPRQYSMAQ IAELVDREIY KKRRHINLPK PILQPLAELI NRVLWWDTGL SRDQVEREFH DQV IDPTAK TFKDLGMEPT DISKWTYHYL LPYRPSTYYD LPPSTEKEMR EERKYLHVLD DQ

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Macromolecule #14: NADH-ubiquinone oxidoreductase-like protein

MacromoleculeName: NADH-ubiquinone oxidoreductase-like protein / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 29.244955 KDa
SequenceString: MRATTRLLAV VRPATQAAAS SASKGKFLVP GAPTGLTGLG THPSPRSALL YLYHSTLEKL KQIPEHSVYR QSVEAVTRHR LALVESVVP EGYDAWVERA KKLLEEHADQ FDPTKVGPEG GEEVQGARAV KVERDGRVFV VSRLPKEEDE RVLEWDGEVD E GPELEGSR ...String:
MRATTRLLAV VRPATQAAAS SASKGKFLVP GAPTGLTGLG THPSPRSALL YLYHSTLEKL KQIPEHSVYR QSVEAVTRHR LALVESVVP EGYDAWVERA KKLLEEHADQ FDPTKVGPEG GEEVQGARAV KVERDGRVFV VSRLPKEEDE RVLEWDGEVD E GPELEGSR TLEEKIEGGL EKIFTRRDVK DTVGRVEWEP EPQLTAEQVA ELENKIGAGL IEEVIQVAEG ELKLVDTMVK AR VWEPLEE QPRPGQWEYF ERKP

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Macromolecule #15: NADH-ubiquinone oxidoreductase 30.4 kDa subunit-like protein

MacromoleculeName: NADH-ubiquinone oxidoreductase 30.4 kDa subunit-like protein
type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 33.377098 KDa
SequenceString: MASKLCKTRA LASALRPISR SSRSTEAIAN VAVVRSFATT PRLGVALPKD APNPRAIPRE PVGEIKQALV NPADKYQSKA DNLHKYGAW LMSCLPKYIQ QFSVWKDELT IYICPTGVIP VFSFLKYNTN AEFTQVSDIT AVDFPTREMR FEIVYNLLSV R HNARIRVK ...String:
MASKLCKTRA LASALRPISR SSRSTEAIAN VAVVRSFATT PRLGVALPKD APNPRAIPRE PVGEIKQALV NPADKYQSKA DNLHKYGAW LMSCLPKYIQ QFSVWKDELT IYICPTGVIP VFSFLKYNTN AEFTQVSDIT AVDFPTREMR FEIVYNLLSV R HNARIRVK TYADEVTPVP SITSLYMGAN WYEREVYDMF GVLFVGHPDL RRIMTDYGFE GHPLRKDFPL TGYTEIRYDE EK KRIVVEP LELTQAFRNF EGGSSAWDQV GPGIDRTPDT FKLPTPKPEE QSEEKK

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Macromolecule #16: Subunit NDUFV2 of NADH-ubiquinone oxidoreductase (Complex I)

MacromoleculeName: Subunit NDUFV2 of NADH-ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 35.005953 KDa
SequenceString: MRRRQLSLQA GPFAEPKANQ ARKSSSNGER SSTTSQDHDP VRPGIDAAIA RSLDTTAAMA SKLTPLLMRT VARMGSRAMW AMVPAPART LSTSAMRHSD TLMVHRNTPE NNPDIPFKFT PENEKIIEQI LKRYPPQYKK AAVMPLLDLG QRQHGFCSIS V MNEVARIL ...String:
MRRRQLSLQA GPFAEPKANQ ARKSSSNGER SSTTSQDHDP VRPGIDAAIA RSLDTTAAMA SKLTPLLMRT VARMGSRAMW AMVPAPART LSTSAMRHSD TLMVHRNTPE NNPDIPFKFT PENEKIIEQI LKRYPPQYKK AAVMPLLDLG QRQHGFCSIS V MNEVARIL EMPPMRVYEV ASFYTMYNRT PVGKFHVQAC TTTPCQLGGC GSDAIVKAIK EHLGINQGET TPDGLFTFIE VE CLGACVN APMVQINDDY YEDLTPETIK QVLTALKESV NDVSKAPKPG PQSGRQSCEN SAGLTSLTSE PWGPEKTRPD L

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Macromolecule #17: Oxidoreductase-like protein

MacromoleculeName: Oxidoreductase-like protein / type: protein_or_peptide / ID: 17 / Details: Sequence renumbered (compared to entry sequence). / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 25.415252 KDa
SequenceString: MLPTPAALLV TRQLPLARVP STLVRTLPIQ ALIARRTYAT PAGPPPKNFR LPPPKNWDEE SESTIDKVGK YFLMTEMLRG MYVLLEQFF RPPYTIYYPF EKGPISPRFR GEHALRRYPS GEERCIACKL CEAVCPAQAI TIEAEERADG SRRTTRYDID M TKCIYCGF ...String:
MLPTPAALLV TRQLPLARVP STLVRTLPIQ ALIARRTYAT PAGPPPKNFR LPPPKNWDEE SESTIDKVGK YFLMTEMLRG MYVLLEQFF RPPYTIYYPF EKGPISPRFR GEHALRRYPS GEERCIACKL CEAVCPAQAI TIEAEERADG SRRTTRYDID M TKCIYCGF CQESCPVDAI VESPNAEYAT ETREELLYNK EKLLANGDKW EPELAAAIRA DAPYR

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Macromolecule #18: NADH-ubiquinone oxidoreductase-like protein

MacromoleculeName: NADH-ubiquinone oxidoreductase-like protein / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 21.612625 KDa
SequenceString: MAPIEEEHEH YHPKDAVHLG LKGAAVVGGI GLLFAAVRTS LARKNVGPWA IFTRNGKLAA TFAAVGGAYD FTRAAAANLR EKEDWVNNG IGGLFAGATM GLTTGRIPRV LGFAALTGVV LATAEYAGSG LRGVFKRDVD EYERKEFLRK NRRRPIEETL A EIGEGRGI ...String:
MAPIEEEHEH YHPKDAVHLG LKGAAVVGGI GLLFAAVRTS LARKNVGPWA IFTRNGKLAA TFAAVGGAYD FTRAAAANLR EKEDWVNNG IGGLFAGATM GLTTGRIPRV LGFAALTGVV LATAEYAGSG LRGVFKRDVD EYERKEFLRK NRRRPIEETL A EIGEGRGI KPPGYYERRA QRLKEKYGVD INPVCADPDQ A

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Macromolecule #19: NADH-ubiquinone oxidoreductase-like protein

MacromoleculeName: NADH-ubiquinone oxidoreductase-like protein / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 25.549582 KDa
SequenceString: MISSARTGAS LILRARPATQ LIPLRAAAAA HLSSSARTQA AATSSSTAVE PYPGYELRKS LRKEVPLPSQ EGTKGLVQYA LTTLDIITN WARQGSFWPM TFGLACCAVE MMHLSTPRYD QDRLGIIFRA SPRQSDVMIV AGTLTNKMAP ALRQVYDQMP D PRWVISMG ...String:
MISSARTGAS LILRARPATQ LIPLRAAAAA HLSSSARTQA AATSSSTAVE PYPGYELRKS LRKEVPLPSQ EGTKGLVQYA LTTLDIITN WARQGSFWPM TFGLACCAVE MMHLSTPRYD QDRLGIIFRA SPRQSDVMIV AGTLTNKMAP ALRQVYDQMP D PRWVISMG SCANGGGYYH YSYSVVRGCD RIVPVDVYVP GCPPTSEALM YGIFQLQRKM RNTKITRMWY RK

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Macromolecule #20: NADH-ubiquinone oxidoreductase chain 4L

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 4L / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 9.837997 KDa
SequenceString:
MNITLILFLI GILGFVLNRK NIILMLISIE IMLLSITFLI LLSSLNMDDI IGQTYAIYII VVAGAESAIG LGILVAFYRL RGSIAIEYK

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Macromolecule #21: NADH-ubiquinone oxidoreductase-like protein

MacromoleculeName: NADH-ubiquinone oxidoreductase-like protein / type: protein_or_peptide / ID: 21
Details: Discrepancy with annotated entry sequence. Probably wrong intron-exon boundaries. Amino acids added and removed according to map density, genome sequence and MS data.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 18.613875 KDa
SequenceString:
MYASRGVRAV AQARQLISKR TFTATARQLS QTQTPTPAAP TATAEAEVAE TGRPASAVMQ APNRAEVWSR SQRPRSEAMA GPRFEQTDF DAQPRPWAAI ELIHKQPVRW THDRIVACDG GGGPHGHPKI YINTDKPEIA TCNYCGLPFA NEHHRKYLES L PQTSYPLN

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Macromolecule #22: Acyl carrier protein

MacromoleculeName: Acyl carrier protein / type: protein_or_peptide / ID: 22 / Details: ZMP covalently linked to Ser98. / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 15.593649 KDa
SequenceString:
MFRSAVLRSA AAATRTTIRS IPPAAAKKFA VAPVSRVTSF IPKTASWQVI RCYASNEGLQ KVEVYERIKS LLAGFDKVND PNNITETAH FANDLGLDSL DTVEVVMAIE EEFSIEIPDK DADQIHSVDK AIEYILRQPD AH

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Macromolecule #23: NADH-ubiquinone oxidoreductase B14 subunit-like protein

MacromoleculeName: NADH-ubiquinone oxidoreductase B14 subunit-like protein
type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 14.836945 KDa
SequenceString:
MAVQPTKFAV TVRQSRNWAD AKQRVIAAYR AWIRAAPEIQ TMYSIPFPVS HIRTRIRQEF ERHRYVNKLN VVDVLLQQSN ADFQETMNF WRQQSHVMAF FKEEHFRGEK RLPSNFITGF LEGRN

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Macromolecule #24: Complex I-B22

MacromoleculeName: Complex I-B22 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 11.636524 KDa
SequenceString:
MSNRQAALSL YRRALKLALD WTVHRNLWRG QALYIRSLFE KNRNVTDPRL QRALLKETEK LLEKWKHPDP YCPPTAPGGS KYERNLPVP NLEPPPPLKF

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Macromolecule #25: Complex I-ESSS

MacromoleculeName: Complex I-ESSS / type: protein_or_peptide / ID: 25
Details: Sequence renumbered (compared to entry sequence) according to map density and genome sequence.
Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 15.847807 KDa
SequenceString:
MDGGPPTFAF RPTARQAPGK LSSAPVTTRL AAAALSRAST VSSKALTPAR FRFFSTTQRR AGGHGMQYDP PTGWLWGVRP GEKYQNEGW EGPFFYGFWG SLIVFAIAYA YKPDTSIQTW ALEEARRRLE AEGILEDPNP TKKE

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Macromolecule #26: NADH-ubiquinone oxidoreductase

MacromoleculeName: NADH-ubiquinone oxidoreductase / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 21.517605 KDa
SequenceString:
MATRKPAFNQ HVLLDTTPLP DSIPKVKEIG ASSAPLLSAS FFIGARCKDY NDDYMQCKNE NPGRGEFECL KEGRRVTRCA RSVLKDINT HCLEQFRAHW QCLDNNNQQL WQCRPAEWKL NKCVYENLKL EKVIPDQPKN STPVHLRQRQ IFAHHAIPPW E RPFIPGQP EPQLPAGIEI PEKYKNQS

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Macromolecule #27: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 14.039227 KDa
SequenceString:
MPQDMPPPGG YEAVQYKRNL PSRGLFRPRP LLAGAAVLML YGWYKLVKGI REQNELAREK MWARIHLIPL LQAEEDRDHV RRYLADQAR EKGLLGENIK VYNSDRYVRP TFAVTPSKPA QE

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Macromolecule #28: NADH-ubiquinone oxidoreductase-like protein

MacromoleculeName: NADH-ubiquinone oxidoreductase-like protein / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 21.645502 KDa
SequenceString:
MSNTPTQTYQ FPSKTVKTDY PLIDNDPHFT RVIRYARPSD YAHGLAAAAA GPAALWLMER ISPSQVGRGG FAKAMRLAGF IGLAGGFLY FYQRSILRFY GMSENAREVE MDMREMTDRV KAGLPLYGES RLSPAMQGVA ARQSRYSALF FGVMPWFNFV N HNQHGVDT AKYYQQAERE LEAERLAREQ AQQ

+
Macromolecule #29: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial

MacromoleculeName: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 23.494629 KDa
SequenceString: MSSLRPAATS AARLLRNSTA SSRATAVAVM PCRAAHNIHV PVQKERTKED SPLATLPRNA PDYNVPIDIA TSTFTPVPKN VQDGSEENV VPAGLISGAP MELQARTVRI YKPAKPATQS GEKNTQLWRM DWDVLGKGHR WENPLMGWQS SADFMQGTHL T FKTKEDAI ...String:
MSSLRPAATS AARLLRNSTA SSRATAVAVM PCRAAHNIHV PVQKERTKED SPLATLPRNA PDYNVPIDIA TSTFTPVPKN VQDGSEENV VPAGLISGAP MELQARTVRI YKPAKPATQS GEKNTQLWRM DWDVLGKGHR WENPLMGWQS SADFMQGTHL T FKTKEDAI AFAEKQGYEY FVQEPNERHF RPKAYANNFL YSPGKLKHIR TK

+
Macromolecule #30: NADH-ubiquinone oxidoreductase-like protein

MacromoleculeName: NADH-ubiquinone oxidoreductase-like protein / type: protein_or_peptide / ID: 30
Details: Discrepancy with annotated entry sequence. Probably wrong intron-exon boundaries. Amino acids removed and added according to map density and genome sequence.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 21.164697 KDa
SequenceString: MASKAAAAAA SNAVSITKKY TVQSTGIWER IRRALVIDPN RSNGVPLNPY NRNPSPGDNP PLEYTDPVTI PAGDIADNPY WKRDFRRNY PRPSVIAQAQ QVALLSVGSA AQPRVELIGE EGTKALVAAE EEGKEKGVAK YLEEKGAEEA KRVLALTGGL P PTPSGQTM ...String:
MASKAAAAAA SNAVSITKKY TVQSTGIWER IRRALVIDPN RSNGVPLNPY NRNPSPGDNP PLEYTDPVTI PAGDIADNPY WKRDFRRNY PRPSVIAQAQ QVALLSVGSA AQPRVELIGE EGTKALVAAE EEGKEKGVAK YLEEKGAEEA KRVLALTGGL P PTPSGQTM VTGQWDVHKY GLAEEQSYGG SYPCRSFV

+
Macromolecule #31: NADH dehydrogenase (Ubiquinone)-like protein

MacromoleculeName: NADH dehydrogenase (Ubiquinone)-like protein / type: protein_or_peptide / ID: 31
Details: Discrepancy with annotated entry sequence. Probably wrong intron-exon boundaries. Amino acids removed and added according to map density, genome sequence and MS data.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 23.239686 KDa
SequenceString: MLSRRLVRAV APLRSPVLPA ARRLPLIQQR TFLPEAMVGR SKIDEKYPDS DYPTLTDKED PDMNGGYINP PRIKRQFRDP HADWWDKQE RRNFGEPVHE DHDILGMFSP YEYTWITPGK GLFQIGLFIA SFLGLCYVVK LTYPDRVSYP REFEGGLERE L GGAGAVRA ...String:
MLSRRLVRAV APLRSPVLPA ARRLPLIQQR TFLPEAMVGR SKIDEKYPDS DYPTLTDKED PDMNGGYINP PRIKRQFRDP HADWWDKQE RRNFGEPVHE DHDILGMFSP YEYTWITPGK GLFQIGLFIA SFLGLCYVVK LTYPDRVSYP REFEGGLERE L GGAGAVRA FLCLDDEIMW MVSLYCLPAS KLISSPVALQ DKSTS

+
Macromolecule #32: Subunit NDUFC2 of NADH-ubiquinone oxidoreductase (Complex I)

MacromoleculeName: Subunit NDUFC2 of NADH-ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 10.823453 KDa
SequenceString:
MVNRILFWTG FGLAVRFWQL GIEMRPFFNR KSLWAYPLFG GVGASFGYWL QSIDEKQTKM LEERKQAILE KRARRAQRQA EAAATAPSP SAQEA

+
Macromolecule #33: Subunit NDUFB3 of NADH-ubiquinone oxidoreductase (Complex I)

MacromoleculeName: Subunit NDUFB3 of NADH-ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 11.153536 KDa
SequenceString:
MQPTRILRNN GEKPNITGFD MREFLRHTKT PTYDPWERHE AWRYTGRFSR FNRFKGALPG FGIATVAFTA YCVFEHFFLK DDHHGHHGE KEHH

+
Macromolecule #34: Subunit NDUFB10 of NADH-ubiquinone oxidoreductase (Complex I)

MacromoleculeName: Subunit NDUFB10 of NADH-ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 12.514257 KDa
SequenceString:
MPTPESEAFL AKKPQVPPTF DGVDYEDNKR LKQAQDAIIR EQWVQVMMGR LVREELSKCY YREGVNHLEK CGKLRERYLQ LLANAKVKG YLFEQQNYWS KENQQQ

+
Macromolecule #35: Subunit NDUFB2 of NADH-ubiquinone oxidoreductase (Complex I)

MacromoleculeName: Subunit NDUFB2 of NADH-ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 35
Details: No entry (not annotated). Sequence modelled according to map density, genome sequence and MS data.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 5.27815 KDa
SequenceString:
MAGGGQHVSR VHRFLATGLG ASMWFWIFYR AKKDGPVLLG WKHPWD

+
Macromolecule #36: NADH dehydrogenase-like protein

MacromoleculeName: NADH dehydrogenase-like protein / type: protein_or_peptide / ID: 36
Details: Discrepancy with annotated entry sequence. Probably wrong intron-exon boundaries. Amino acid removed according to map density, genome sequence and MS data.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 10.897596 KDa
SequenceString:
MSGRYAFAKG VKELRFLFCQ TSQHSAEARS FLNRAYPIMK KHNPTIPILL REAQGTIPRV YARYEFGREK MESLEGLTDK QIEETVAKL VKTEGA

+
Macromolecule #37: Subunit NDUFA3 of NADH-ubiquinone oxidoreductase (Complex I)

MacromoleculeName: Subunit NDUFA3 of NADH-ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 9.076461 KDa
SequenceString:
MSATTPRFWS TPLKYCRWAA RERPALFFSV VIGALGPVTL ATVPPLRRLI GDVDAAPIPL TYPIPPGPRK QLKGYDDDTE DN

+
Macromolecule #38: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit
type: protein_or_peptide / ID: 38
Details: Sequence renumbered (compared to entry sequence) according to map density, genome sequence and MS data.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 15.967092 KDa
SequenceString:
MSYPTRTLAN LRKIGLKEYF RQLLYIGDTK YGELVGVDKF GNKFYENKEE LPLRTRWVDY AKHDYDAAHI EPMWHAWISY QVDTPPTRE PLTQIERPWA PKEHVPNRSF TRGAYKPYNT TQPKIQSWEP KAAPR

+
Macromolecule #39: Subunit NDUFB6 of NADH-ubiquinone oxidoreductase (Complex I)

MacromoleculeName: Subunit NDUFB6 of NADH-ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 10.857294 KDa
SequenceString:
MGGGPKIPYP KHVWSPAGGW YAQPANWKQN TAIFGLVIFG ITAMVWKYSA EHEVRHKMPE PDRFYPSRYW VKQIKDYERA QKEKQQNNT EASS

+
Macromolecule #40: Subunit NDUFB4 of NADH-ubiquinone oxidoreductase (Complex I)

MacromoleculeName: Subunit NDUFB4 of NADH-ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 8.760336 KDa
SequenceString:
MAGLQHYKIA MDPALVRLGS MISNRYKYFR WTKRTALVSF MYVVVVPSTI GYLAYKTDGL WDLRAKRRGD LISER

+
Macromolecule #41: Subunit NDUFB5 of NADH-ubiquinone oxidoreductase (Complex I)

MacromoleculeName: Subunit NDUFB5 of NADH-ubiquinone oxidoreductase (Complex I)
type: protein_or_peptide / ID: 41
Details: Discrepancy with annotated entry sequence. Probably wrong intron-exon boundaries. Amino acids added and removed according to map density and genome sequence.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 20.824342 KDa
SequenceString:
MLALRQRAAL LARRVRPTVV VPRNARTYAS SHDHDHHDHH HDHGHNVEEP LGAAFYIAVG GIASSFVIYN ISRPGPNGEP SSLHKWFSK ISDYKDEWET RNTLMAAALE QAAHDKHLLL TAERSRHIEL KYPEVFSHGS PFNVPAGFYP NLDHVIEHYR K QHLEEEER KAKKLAAAAA AASEAR

+
Macromolecule #42: Oxidoreductase-like domain-containing protein

MacromoleculeName: Oxidoreductase-like domain-containing protein / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 41.609055 KDa
SequenceString: MRRTILQARP SRVLSRALSS AHGSARPSFA IARSPNEQVF PAGAFYESIL RWNPNPVPKP QPIEPPAPEP EPVVEEATPA EPEPVPEPV AEEKTPEPTP APVEEKSAAP VEAEAEPAPV EEKAAVPEAA PEPEPEPVAE DKPAPEEKKS SRSPKRQQQE E SSSAEKPA ...String:
MRRTILQARP SRVLSRALSS AHGSARPSFA IARSPNEQVF PAGAFYESIL RWNPNPVPKP QPIEPPAPEP EPVVEEATPA EPEPVPEPV AEEKTPEPTP APVEEKSAAP VEAEAEPAPV EEKAAVPEAA PEPEPEPVAE DKPAPEEKKS SRSPKRQQQE E SSSAEKPA EKPIRSSGRL DGASAPSPEP QPQDSSQEKP SITFLSGLSG PTSTSSYQSR LESIRSRSRL IAGVLVPPQP TE PDNCCMS GCVNCVWDAY REEMEAWAAA KAEAERRLAA QEVSSVVGTT EESMHAVEDG KRVERTLGVH GEVADRTGDM ARE LEKGST SMEEDGAGFG ERMEWDEGLY KDVPVGIREF MKQEKRLREE RLKRNRKETE HQQG

+
Macromolecule #43: 1,2-Distearoyl-sn-glycerophosphoethanolamine

MacromoleculeName: 1,2-Distearoyl-sn-glycerophosphoethanolamine / type: ligand / ID: 43 / Number of copies: 17 / Formula: 3PE
Molecular weightTheoretical: 748.065 Da
Chemical component information

ChemComp-3PE:
1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipid*YM / Phosphatidylethanolamine

+
Macromolecule #44: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE

MacromoleculeName: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / type: ligand / ID: 44 / Number of copies: 8 / Formula: PC1
Molecular weightTheoretical: 790.145 Da
Chemical component information

ChemComp-PC1:
1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM / Phosphatidylcholine

+
Macromolecule #45: CARDIOLIPIN

MacromoleculeName: CARDIOLIPIN / type: ligand / ID: 45 / Number of copies: 5 / Formula: CDL
Molecular weightTheoretical: 1.464043 KDa
Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM / Cardiolipin

+
Macromolecule #46: Lauryl Maltose Neopentyl Glycol

MacromoleculeName: Lauryl Maltose Neopentyl Glycol / type: ligand / ID: 46 / Number of copies: 3 / Formula: LMN
Molecular weightTheoretical: 1.005188 KDa
Chemical component information

ChemComp-AV0:
Lauryl Maltose Neopentyl Glycol

+
Macromolecule #47: FE2/S2 (INORGANIC) CLUSTER

MacromoleculeName: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 47 / Number of copies: 2 / Formula: FES
Molecular weightTheoretical: 175.82 Da
Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

+
Macromolecule #48: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 48 / Number of copies: 6 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

+
Macromolecule #49: FLAVIN MONONUCLEOTIDE

MacromoleculeName: FLAVIN MONONUCLEOTIDE / type: ligand / ID: 49 / Number of copies: 1 / Formula: FMN
Molecular weightTheoretical: 456.344 Da
Chemical component information

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE / Flavin mononucleotide

+
Macromolecule #50: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

MacromoleculeName: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
type: ligand / ID: 50 / Number of copies: 1 / Formula: NDP
Molecular weightTheoretical: 745.421 Da
Chemical component information

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / Nicotinamide adenine dinucleotide phosphate

+
Macromolecule #51: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 51 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #52: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-b...

MacromoleculeName: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate
type: ligand / ID: 52 / Number of copies: 2 / Formula: ZMP
Molecular weightTheoretical: 568.704 Da
Chemical component information

ChemComp-ZMP:
S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate

+
Macromolecule #53: water

MacromoleculeName: water / type: ligand / ID: 53 / Number of copies: 1593 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER / Water

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

Concentration1.5 mg/mL
BufferpH: 7.4
Component:
ConcentrationFormulaName
20.0 mMHepes/NaOH4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (pH adjusted with sodium hydroxide)
100.0 mMNaClSodium chloridesodium chloride
0.0015 % [w/v]LMNGlauryl maltose neopentyl glycol
GridModel: C-flat-1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 45 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 105000
Specialist opticsEnergy filter - Name: GIF Bioquantum / Energy filter - Slit width: 30 eV
Sample stageCooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 1 / Number real images: 6503 / Average exposure time: 2.11 sec. / Average electron dose: 45.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Particle selectionNumber selected: 1087651
Initial angle assignmentType: NOT APPLICABLE / Software - Name: cryoSPARC (ver. 3.0.1)
Final angle assignmentType: NOT APPLICABLE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.75 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 3.0.1) / Number images used: 21989
FSC plot (resolution estimation)

-
Atomic model buiding 1

Initial model(PDB ID:

6rfr

,

6rfq

)
Output model

PDB-7zmb:
CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (state 2)

+
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