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TitleStructure and assembly of cargo Rubisco in two native α-carboxysomes.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 4299, Year 2022
Publish dateJul 25, 2022
AuthorsTao Ni / Yaqi Sun / Will Burn / Monsour M J Al-Hazeem / Yanan Zhu / Xiulian Yu / Lu-Ning Liu / Peijun Zhang /
PubMed AbstractCarboxysomes are a family of bacterial microcompartments in cyanobacteria and chemoautotrophs. They encapsulate Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) and carbonic anhydrase ...Carboxysomes are a family of bacterial microcompartments in cyanobacteria and chemoautotrophs. They encapsulate Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) and carbonic anhydrase catalyzing carbon fixation inside a proteinaceous shell. How Rubisco complexes pack within the carboxysomes is unknown. Using cryo-electron tomography, we determine the distinct 3D organization of Rubisco inside two distant α-carboxysomes from a marine α-cyanobacterium Cyanobium sp. PCC 7001 where Rubiscos are organized in three concentric layers, and from a chemoautotrophic bacterium Halothiobacillus neapolitanus where they form intertwining spirals. We further resolve the structures of native Rubisco as well as its higher-order assembly at near-atomic resolutions by subtomogram averaging. The structures surprisingly reveal that the authentic intrinsically disordered linker protein CsoS2 interacts with Rubiscos in native carboxysomes but functions distinctively in the two α-carboxysomes. In contrast to the uniform Rubisco-CsoS2 association in the Cyanobium α-carboxysome, CsoS2 binds only to the Rubiscos close to the shell in the Halo α-carboxysome. Our findings provide critical knowledge of the assembly principles of α-carboxysomes, which may aid in the rational design and repurposing of carboxysome structures for new functions.
External linksNat Commun / PubMed:35879301 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution3.3 - 4.8 Å
Structure data

EMDB-14589: Subtomogram averaging structure of tandem Rubiscos from H. neapolitanus carboxysomes
Method: EM (subtomogram averaging) / Resolution: 3.3 Å

14590

7zbt

EMDB-14590, PDB-7zbt:
Subtomogram averaging of Rubisco from native Halothiobacillus carboxysomes
Method: EM (subtomogram averaging) / Resolution: 3.3 Å

EMDB-14592: Subtomogram averaging of Rubiscos along the shell of Halothiobacillus carboxysome
Method: EM (subtomogram averaging) / Resolution: 3.82 Å

EMDB-14593: Subtomogram averaging of Rubiscos within 30 nm from center of Halothiobacillus carboxysome
Method: EM (subtomogram averaging) / Resolution: 4.2 Å

14617

7zc1

EMDB-14617, PDB-7zc1:
Subtomogram averaging of Rubisco from Cyanobium carboxysome
Method: EM (subtomogram averaging) / Resolution: 3.8 Å

EMDB-14623: Subtomogram averaging of Rubisco from Cyanobium carboxysome (innermost layer)
Method: EM (subtomogram averaging) / Resolution: 4.8 Å

EMDB-14624: Subtomogram averaging of Rubisco from Cyanobium carboxysome (middle layer)
Method: EM (subtomogram averaging) / Resolution: 4.4 Å

EMDB-14625: Subtomogram averaging of Rubisco from Cyanobium carboxysome (outerlayer)
Method: EM (subtomogram averaging) / Resolution: 4.28 Å

Source
  • halothiobacillus neapolitanus (bacteria)
  • cyanobium sp. pcc 7001 (bacteria)
KeywordsUNKNOWN FUNCTION / Rubisco / carboxysome / alpha carboxysomes

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