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TitleCryo-EM Structures of Two Bacteriophage Portal Proteins Provide Insights for Antimicrobial Phage Engineering.
Journal, issue, pagesViruses, Vol. 13, Issue 12, Year 2021
Publish dateDec 16, 2021
AuthorsAbid Javed / Hugo Villanueva / Shadikejiang Shataer / Sara Vasciaveo / Renos Savva / Elena V Orlova /
PubMed AbstractWidespread antibiotic resistance has returned attention to bacteriophages as a means of managing bacterial pathogenesis. Synthetic biology approaches to engineer phages have demonstrated genomic ...Widespread antibiotic resistance has returned attention to bacteriophages as a means of managing bacterial pathogenesis. Synthetic biology approaches to engineer phages have demonstrated genomic editing to broaden natural host ranges, or to optimise microbicidal action. Gram positive pathogens cause serious pastoral animal and human infections that are especially lethal in newborns. Such pathogens are targeted by the obligate lytic phages of the and families. These phages have relatively small ~20 kb linear protein-capped genomes and their compact organisation, relatively few structural elements, and broad host range, are appealing from a phage-engineering standpoint. In this study, we focus on portal proteins, which are core elements for the assembly of such tailed phages. The structures of dodecameric portal complexes from phage GA1, which targets , and phage phiCPV4 that infects , were determined at resolutions of 3.3 Å and 2.9 Å, respectively. Both are found to closely resemble the related phi29 portal protein fold. However, the portal protein of phiCPV4 exhibits interesting differences in the clip domain. These structures provide new insights on structural diversity in portal proteins and will be essential for considerations in phage structural engineering.
External linksViruses / PubMed:34960800 / PubMed Central
MethodsEM (single particle)
Resolution2.8 - 3.2 Å
Structure data

13664

7pv2

EMDB-13664, PDB-7pv2:
GA1 bacteriophage portal protein
Method: EM (single particle) / Resolution: 3.2 Å

13665

7pv4

EMDB-13665, PDB-7pv4:
PhiCPV4 bacteriophage Portal Protein
Method: EM (single particle) / Resolution: 2.8 Å

Source
  • bacillus phage ga-1 (virus)
  • Clostridium perfringens (bacteria)
  • clostridium phage phicpv4 (virus)
KeywordsVIRAL PROTEIN / Bacteriophage / Portal protein / cryoEM

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