EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure and dynamics of a mycobacterial type VII secretion system.
ジャーナル・号・ページ	Nature, Vol. 593, Issue 7859, Page 445-448, Year 2021
掲載日	2021年5月12日
著者	Catalin M Bunduc / Dirk Fahrenkamp / Jiri Wald / Roy Ummels / Wilbert Bitter / Edith N G Houben / Thomas C Marlovits /
PubMed 要旨	Mycobacterium tuberculosis is the cause of one of the most important infectious diseases in humans, which leads to 1.4 million deaths every year. Specialized protein transport systems-known as ...Mycobacterium tuberculosis is the cause of one of the most important infectious diseases in humans, which leads to 1.4 million deaths every year. Specialized protein transport systems-known as type VII secretion systems (T7SSs)-are central to the virulence of this pathogen, and are also crucial for nutrient and metabolite transport across the mycobacterial cell envelope. Here we present the structure of an intact T7SS inner-membrane complex of M. tuberculosis. We show how the 2.32-MDa ESX-5 assembly, which contains 165 transmembrane helices, is restructured and stabilized as a trimer of dimers by the MycP protease. A trimer of MycP caps a central periplasmic dome-like chamber that is formed by three EccB dimers, with the proteolytic sites of MycP facing towards the cavity. This chamber suggests a central secretion and processing conduit. Complexes without MycP show disruption of the EccB periplasmic assembly and increased flexibility, which highlights the importance of MycP for complex integrity. Beneath the EccB-MycP chamber, dimers of the EccC ATPase assemble into three bundles of four transmembrane helices each, which together seal the potential central secretion channel. Individual cytoplasmic EccC domains adopt two distinctive conformations that probably reflect different secretion states. Our work suggests a previously undescribed mechanism of protein transport and provides a structural scaffold to aid in the development of drugs against this major human pathogen.
リンク	Nature / PubMed:33981042 / PubMed Central
手法	EM (単粒子)
解像度	3.27 - 7.58 Å
構造データ	12514 7np7 EMDB-12514: Structure of an intact ESX-5 inner membrane complex, C1 symmetry PDB-7np7: Structure of an intact ESX-5 inner membrane complex, Composite C1 model 手法: EM (単粒子) / 解像度: 4.03 Å 12517 7npr EMDB-12517: Structure of an intact ESX-5 inner membrane complex, C3 symmetry PDB-7npr: Structure of an intact ESX-5 inner membrane complex, Composite C3 model 手法: EM (単粒子) / 解像度: 3.82 Å 12518 7nps EMDB-12518: Structure of the periplasmic assembly from the ESX-5 inner membrane complex, C1 symmetry PDB-7nps: Structure of the periplasmic assembly from the ESX-5 inner membrane complex, C1 model 手法: EM (単粒子) / 解像度: 3.81 Å EMDB-12519: Periplasmic assembly of the intact ESX-5 inner membrane complex, C3 symmetry 手法: EM (単粒子) / 解像度: 3.52 Å 12520 7npt EMDB-12520, PDB-7npt: Cytosolic bridge of an intact ESX-5 inner membrane complex 手法: EM (単粒子) / 解像度: 3.27 Å 12521 7npu EMDB-12521, PDB-7npu: MycP5-free ESX-5 inner membrane complex, state I 手法: EM (単粒子) / 解像度: 4.48 Å 12522 7npv EMDB-12522, PDB-7npv: MycP5-free ESX-5 inner membrane complex, State II 手法: EM (単粒子) / 解像度: 6.66 Å EMDB-12523: Structure of an intact ESX-5 inner membrane complex, EccC5 extended 手法: EM (単粒子) / 解像度: 4.27 Å EMDB-12525: Structure of an intact ESX-5 inner membrane complex, EccC contracted 手法: EM (単粒子) / 解像度: 7.58 Å
由来	Mycobacterium tuberculosis H37Rv (結核菌) mycobacterium tuberculosis (strain atcc 25618 / h37rv) (結核菌)
キーワード	MEMBRANE PROTEIN / T7SS / mycobacteria / protein transport / secretion / type VII secretion system / membrane