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TitleStructural and molecular determinants for the interaction of ExbB from Serratia marcescens and HasB, a TonB paralog.
Journal, issue, pagesCommun Biol, Vol. 5, Issue 1, Page 355, Year 2022
Publish dateApr 13, 2022
AuthorsValérie Biou / Ricardo Jorge Diogo Adaixo / Mohamed Chami / Pierre-Damien Coureux / Benoist Laurent / Véronique Yvette Ntsogo Enguéné / Gisele Cardoso de Amorim / Nadia Izadi-Pruneyre / Christian Malosse / Julia Chamot-Rooke / Henning Stahlberg / Philippe Delepelaire /
PubMed AbstractExbB and ExbD are cytoplasmic membrane proteins that associate with TonB to convey the energy of the proton-motive force to outer membrane receptors in Gram-negative bacteria for iron uptake. The ...ExbB and ExbD are cytoplasmic membrane proteins that associate with TonB to convey the energy of the proton-motive force to outer membrane receptors in Gram-negative bacteria for iron uptake. The opportunistic pathogen Serratia marcescens (Sm) possesses both TonB and a heme-specific TonB paralog, HasB. ExbB has a long periplasmic extension absent in other bacteria such as E. coli (Ec). Long ExbB's are found in several genera of Alphaproteobacteria, most often in correlation with a hasB gene. We investigated specificity determinants of ExbB and HasB. We determined the cryo-EM structures of ExbB and of the ExbB-ExbD complex from S. marcescens. ExbB alone is a stable pentamer, and its complex includes two ExbD monomers. We showed that ExbB extension interacts with HasB and is involved in heme acquisition and we identified key residues in the membrane domain of ExbB and ExbB, essential for function and likely involved in the interaction with TonB/HasB. Our results shed light on the class of inner membrane energy machinery formed by ExbB, ExbD and HasB.
External linksCommun Biol / PubMed:35418619 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 4.0 Å
Structure data

10789

6ye4

EMDB-10789, PDB-6ye4:
Structure of ExbB pentamer from Serratia marcescens by single particle cryo electron microscopy
Method: EM (single particle) / Resolution: 3.2 Å

11806

7ajq

EMDB-11806, PDB-7ajq:
cryo-EM structure of ExbBD from Serratia Marcescens
Method: EM (single particle) / Resolution: 4.0 Å

Chemicals

ChemComp-PGT:
(1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE / phospholipid*YM / Phosphatidylglycerol

Source
  • serratia marcescens (bacteria)
KeywordsMETAL TRANSPORT / membrane protein / iron uptake / proton transfer / TonB complex / MOTOR PROTEIN / TonB transport

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