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- PDB-7ajq: cryo-EM structure of ExbBD from Serratia Marcescens -

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Basic information

Entry
Database: PDB / ID: 7ajq
Titlecryo-EM structure of ExbBD from Serratia Marcescens
Components
  • Biopolymer transport protein ExbB
  • Biopolymer transport protein ExbD
KeywordsMOTOR PROTEIN / TonB transport / MEMBRANE PROTEIN / IRON UPTAKE / PROTON TRANSFER / TONB COMPLEX / METAL TRANSPORT
Function / homology
Function and homology information


transmembrane transporter activity / protein transport / membrane => GO:0016020 / plasma membrane
Similarity search - Function
TonB-system energizer ExbB type-1 / TonB system transport protein ExbD type-1 / Biopolymer transport protein ExbD/TolR / Biopolymer transport protein ExbD/TolR / MotA/TolQ/ExbB proton channel / MotA/TolQ/ExbB proton channel family
Similarity search - Domain/homology
Biopolymer transport protein ExbB / Biopolymer transport protein ExbD
Similarity search - Component
Biological speciesSerratia marcescens (bacteria)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4 Å
AuthorsBiou, V. / Adaixo, R. / Coureux, P.D. / Delepelaire, P. / Chami, M.
Funding support France, 1items
OrganizationGrant numberCountry
Agence Nationale de la Recherche (ANR)11-LABX-0011 France
Citation
Journal: Commun Biol / Year: 2022
Title: Structural and molecular determinants for the interaction of ExbB from Serratia marcescens and HasB, a TonB paralog.
Authors: Valérie Biou / Ricardo Jorge Diogo Adaixo / Mohamed Chami / Pierre-Damien Coureux / Benoist Laurent / Véronique Yvette Ntsogo Enguéné / Gisele Cardoso de Amorim / Nadia Izadi-Pruneyre / ...Authors: Valérie Biou / Ricardo Jorge Diogo Adaixo / Mohamed Chami / Pierre-Damien Coureux / Benoist Laurent / Véronique Yvette Ntsogo Enguéné / Gisele Cardoso de Amorim / Nadia Izadi-Pruneyre / Christian Malosse / Julia Chamot-Rooke / Henning Stahlberg / Philippe Delepelaire /
Abstract: ExbB and ExbD are cytoplasmic membrane proteins that associate with TonB to convey the energy of the proton-motive force to outer membrane receptors in Gram-negative bacteria for iron uptake. The ...ExbB and ExbD are cytoplasmic membrane proteins that associate with TonB to convey the energy of the proton-motive force to outer membrane receptors in Gram-negative bacteria for iron uptake. The opportunistic pathogen Serratia marcescens (Sm) possesses both TonB and a heme-specific TonB paralog, HasB. ExbB has a long periplasmic extension absent in other bacteria such as E. coli (Ec). Long ExbB's are found in several genera of Alphaproteobacteria, most often in correlation with a hasB gene. We investigated specificity determinants of ExbB and HasB. We determined the cryo-EM structures of ExbB and of the ExbB-ExbD complex from S. marcescens. ExbB alone is a stable pentamer, and its complex includes two ExbD monomers. We showed that ExbB extension interacts with HasB and is involved in heme acquisition and we identified key residues in the membrane domain of ExbB and ExbB, essential for function and likely involved in the interaction with TonB/HasB. Our results shed light on the class of inner membrane energy machinery formed by ExbB, ExbD and HasB.
#1: Journal: Biorxiv / Year: 2021
Title: Functional and structural characterization of Serratia marcescens ExbB: determinants of the interaction with HasB/TonB
Authors: Biou, V. / Chami, M. / Coureux, P.D. / Laurent, B. / Ntsogo, Y. / Izadi-Pruneyre, N. / Malosse, C. / Chamot-Rooke, J. / Stahlberg, H. / Delepelaire, P.
History
DepositionSep 29, 2020Deposition site: PDBE / Processing site: PDBE
Revision 1.0Oct 6, 2021Provider: repository / Type: Initial release
Revision 1.1Oct 13, 2021Group: Data collection / Database references
Category: citation / citation_author ...citation / citation_author / em_admin / pdbx_database_proc
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.pdbx_database_id_DOI / _citation.title / _citation.year / _em_admin.last_update
Revision 1.2May 4, 2022Group: Database references / Category: citation / citation_author
Revision 1.3Jul 10, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / citation / em_3d_fitting_list / em_admin / pdbx_initial_refinement_model
Item: _citation.journal_id_ISSN / _em_3d_fitting_list.accession_code ..._citation.journal_id_ISSN / _em_3d_fitting_list.accession_code / _em_3d_fitting_list.initial_refinement_model_id / _em_3d_fitting_list.source_name / _em_3d_fitting_list.type / _em_admin.last_update

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Structure visualization

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Structure viewerMolecule:
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Assembly

Deposited unit
A: Biopolymer transport protein ExbB
B: Biopolymer transport protein ExbB
C: Biopolymer transport protein ExbB
D: Biopolymer transport protein ExbB
E: Biopolymer transport protein ExbB
F: Biopolymer transport protein ExbD
G: Biopolymer transport protein ExbD


Theoretical massNumber of molelcules
Total (without water)180,4617
Polymers180,4617
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_5551
Buried area21400 Å2
ΔGint-194 kcal/mol
Surface area59460 Å2
MethodPISA

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Components

#1: Protein
Biopolymer transport protein ExbB


Mass: 29584.752 Da / Num. of mol.: 5
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Serratia marcescens (bacteria) / Gene: FHU12_5200 / Plasmid: pBAD24 / Production host: Escherichia coli (E. coli) / Strain (production host): XL1-Blue / References: UniProt: A0A542C9I8
#2: Protein Biopolymer transport protein ExbD


Mass: 16268.690 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Serratia marcescens (bacteria)
Gene: exbD, A8A12_13035, AN695_0216350, AR325_24260, BVG93_18405, BVG97_18665, DMW43_16865, E4655_06935, EGJ31_09425, FG174_21750, FOT62_00910, FR965_26870, G3M87_18025, G3M88_18025, G3M89_17265, ...Gene: exbD, A8A12_13035, AN695_0216350, AR325_24260, BVG93_18405, BVG97_18665, DMW43_16865, E4655_06935, EGJ31_09425, FG174_21750, FOT62_00910, FR965_26870, G3M87_18025, G3M88_18025, G3M89_17265, GMA22_18535, GST50_08875, GV243_22030, SMATCC274_40720
Plasmid: pBAD24 / Production host: Escherichia coli (E. coli) / Strain (production host): XL1-blue / References: UniProt: V5YUQ0

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: 5-ExbB + 2-ExbD complex / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT
Molecular weightValue: 0.15 MDa / Experimental value: NO
Source (natural)Organism: Serratia marcescens (bacteria)
Source (recombinant)Organism: Escherichia coli (E. coli) / Strain: XL1-blue / Plasmid: pBAD24
Buffer solutionpH: 8
Buffer component
IDConc.NameFormulaBuffer-ID
1100 mMsodium chlorideNaCl1
220 mMtrisTris-HCl1
30.0015 %Lauryl Maltose Neopentyl GlycolLMNG1
SpecimenConc.: 0.1 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Details: the sample was monodisperse as seen on gel filtration chromatogram
Specimen supportGrid material: COPPER / Grid mesh size: 200 divisions/in. / Grid type: C-flat
VitrificationInstrument: FEI VITROBOT MARK II / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 293 K

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal magnification: 139000 X / Nominal defocus max: 2500 nm / Nominal defocus min: 1000 nm / Calibrated defocus min: 1000 nm / Calibrated defocus max: 2800 nm / Cs: 2.7 mm / Alignment procedure: BASIC
Specimen holderCryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Temperature (max): 100 K / Temperature (min): 100 K
Image recordingAverage exposure time: 12 sec. / Electron dose: 4.58 e/Å2 / Detector mode: SUPER-RESOLUTION / Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Num. of grids imaged: 1 / Num. of real images: 4000
Image scansMovie frames/image: 48 / Used frames/image: 1-48

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Processing

Software
NameVersionClassification
phenix.real_space_refine1.17.1_3660refinement
PHENIX1.17.1_3660refinement
EM software
IDNameVersionCategory
2EPUlatestimage acquisition
4Gctf1.06CTF correction
7Coot0.8model fitting
9cryoSPARC2initial Euler assignment
10cryoSPARC2final Euler assignment
11cryoSPARC2classification
12cryoSPARC23D reconstruction
13PHENIX1.17.1model refinement
CTF correctionType: NONE
Particle selectionNum. of particles selected: 1373000 / Details: picking on 3028 images with best resolution
SymmetryPoint symmetry: C1 (asymmetric)
3D reconstructionResolution: 4 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 158000 / Num. of class averages: 1 / Symmetry type: POINT
Atomic model buildingB value: 169 / Protocol: RIGID BODY FIT / Space: REAL / Target criteria: correlation coefficient
Atomic model buildingPDB-ID: 6YE4

6ye4


Pdb chain-ID: A / Accession code: 6YE4 / Source name: PDB / Type: experimental model
RefinementCross valid method: NONE
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
Displacement parametersBiso mean: 168.3 Å2
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.00219340
ELECTRON MICROSCOPYf_angle_d0.523412657
ELECTRON MICROSCOPYf_chiral_restr0.03491502
ELECTRON MICROSCOPYf_plane_restr0.00281642
ELECTRON MICROSCOPYf_dihedral_angle_d23.03561320

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