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Structure paper

TitleStructure of a mitochondrial ATP synthase with bound native cardiolipin.
Journal, issue, pagesElife, Vol. 8, Year 2019
Publish dateNov 18, 2019
AuthorsAlexander Mühleip / Sarah E McComas / Alexey Amunts /
PubMed AbstractThe mitochondrial ATP synthase fuels eukaryotic cells with chemical energy. Here we report the cryo-EM structure of a divergent ATP synthase dimer from mitochondria of , a member of the phylum ...The mitochondrial ATP synthase fuels eukaryotic cells with chemical energy. Here we report the cryo-EM structure of a divergent ATP synthase dimer from mitochondria of , a member of the phylum Euglenozoa that also includes human parasites. It features 29 different subunits, 8 of which are newly identified. The membrane region was determined to 2.8 Å resolution, enabling the identification of 37 associated lipids, including 25 cardiolipins, which provides insight into protein-lipid interactions and their functional roles. The rotor-stator interface comprises four membrane-embedded horizontal helices, including a distinct subunit . The dimer interface is formed entirely by phylum-specific components, and a peripherally associated subcomplex contributes to the membrane curvature. The central and peripheral stalks directly interact with each other. Last, the ATPase inhibitory factor 1 (IF) binds in a mode that is different from human, but conserved in Trypanosomatids.
External linksElife / PubMed:31738165 / PubMed Central
MethodsEM (single particle)
Resolution2.8 - 4.32 Å
Structure data

10467

6tdu

EMDB-10467, PDB-6tdu:
Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, full dimer, rotational states 1
Method: EM (single particle) / Resolution: 4.32 Å

10468

6tdv

EMDB-10468, PDB-6tdv:
Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, membrane region
Method: EM (single particle) / Resolution: 2.8 Å

10469

6tdw

EMDB-10469, PDB-6tdw:
Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, peripheral stalk, rotational state 1
Method: EM (single particle) / Resolution: 3.8 Å

10470

6tdx

EMDB-10470, PDB-6tdx:
Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, rotor, rotational state 1
Method: EM (single particle) / Resolution: 3.3 Å

10471

6tdy

EMDB-10471: Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, OSCP/F1/cring in rotational state 1
PDB-6tdy: Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, OSCP/F1/c-ring in rotational state 1
Method: EM (single particle) / Resolution: 3.04 Å

10472

6tdz

EMDB-10472: Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, OSCP/F1/cring rotational state 2
PDB-6tdz: Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, OSCP/F1/c-ring, rotational state 2
Method: EM (single particle) / Resolution: 3.14 Å

10473

6te0

EMDB-10473, PDB-6te0:
Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, OSCP/F1/c-ring, rotational state 3
Method: EM (single particle) / Resolution: 3.92 Å

EMDB-10474:
Cryo-EM structure of the Euglena gracilis mitochondrial ATP synthase, OSCP/F1/c-ring, rotational state 3 with bound IF1
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-10475:
Cryo-EM structure of the Euglena gracilis mitochondrial ATP synthase, OSCP/F1/c-ring, rotational state 3 without IF1
Method: EM (single particle) / Resolution: 4.2 Å

Chemicals

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

ChemComp-LPP:
2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE / phospholipid*YM

ChemComp-TRT:
FRAGMENT OF TRITON X-100 / detergent*YM

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Source
  • euglena gracilis (euglena)
KeywordsMEMBRANE PROTEIN / mitochondria / ATP synthase

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