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TitleCryo-EM structure of the human heteromeric amino acid transporter bAT-rBAT.
Journal, issue, pagesSci Adv, Vol. 6, Issue 16, Page eaay6379, Year 2020
Publish dateApr 15, 2020
AuthorsRenhong Yan / Yaning Li / Yi Shi / Jiayao Zhou / Jianlin Lei / Jing Huang / Qiang Zhou /
PubMed AbstractHeteromeric amino acid transporters (HATs) catalyze the transmembrane movement of amino acids, comprising two subunits, a heavy chain and a light chain, linked by a disulfide bridge. The bAT (SLC7A9) ...Heteromeric amino acid transporters (HATs) catalyze the transmembrane movement of amino acids, comprising two subunits, a heavy chain and a light chain, linked by a disulfide bridge. The bAT (SLC7A9) is a representative light chain of HATs, forming heterodimer with rBAT, a heavy chain which mediates the membrane trafficking of bAT. The bAT-rBAT complex is an obligatory exchanger, which mediates the influx of cystine and cationic amino acids and the efflux of neutral amino acids in kidney and small intestine. Here, we report the cryo-EM structure of the human bAT-rBAT complex alone and in complex with arginine substrate at resolution of 2.7 and 2.3 Å, respectively. The overall structure of bAT-rBAT exists as a dimer of heterodimer consistent with the previous study. A ligand molecule is bound to the substrate binding pocket, near which an occluded pocket is identified, to which we found that it is important for substrate transport.
External linksSci Adv / PubMed:32494597 / PubMed Central
MethodsEM (single particle)
Resolution2.3 - 2.9 Å
Structure data

0903

6li9

EMDB-0903, PDB-6li9:
Heteromeric amino acid transporter b0,+AT-rBAT complex bound with Arginine
Method: EM (single particle) / Resolution: 2.3 Å

0904

6lid

EMDB-0904, PDB-6lid:
Heteromeric amino acid transporter b0,+AT-rBAT complex
Method: EM (single particle) / Resolution: 2.7 Å

EMDB-0905:
cryo EM map of b0,+AT-rBAT complex, focused refined on soluble domain
Method: EM (single particle) / Resolution: 2.5 Å

EMDB-0906:
Cryo EM map of b0,+AT-rBAT complex, focused refined on TM domain
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-0907:
Cryo EM map of b0,+AT-rBAT complex bound with Arginine, focused refined on soluble domain
Method: EM (single particle) / Resolution: 2.3 Å

EMDB-0908:
Cryo EM map of b0,+ AT-rBAT complex bound with Arginine, focused refined on TM domain
Method: EM (single particle) / Resolution: 2.6 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-ARG:
ARGININE

ChemComp-3PH:
1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / transporter

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