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TitleThe basic keratin 10-binding domain of the virulence-associated pneumococcal serine-rich protein PsrP adopts a novel MSCRAMM fold.
Journal, issue, pagesOpen Biol, Vol. 4, Issue 1, Page 130090, Year 2014
Publish dateJan 15, 2014
AuthorsTim Schulte / Jonas Löfling / Cecilia Mikaelsson / Alexey Kikhney / Karina Hentrich / Aurora Diamante / Christine Ebel / Staffan Normark / Dmitri Svergun / Birgitta Henriques-Normark / Adnane Achour /
PubMed AbstractStreptococcus pneumoniae is a major human pathogen, and a leading cause of disease and death worldwide. Pneumococcal invasive disease is triggered by initial asymptomatic colonization of the human ...Streptococcus pneumoniae is a major human pathogen, and a leading cause of disease and death worldwide. Pneumococcal invasive disease is triggered by initial asymptomatic colonization of the human upper respiratory tract. The pneumococcal serine-rich repeat protein (PsrP) is a lung-specific virulence factor whose functional binding region (BR) binds to keratin-10 (KRT10) and promotes pneumococcal biofilm formation through self-oligomerization. We present the crystal structure of the KRT10-binding domain of PsrP (BR187-385) determined to 2.0 Å resolution. BR187-385 adopts a novel variant of the DEv-IgG fold, typical for microbial surface components recognizing adhesive matrix molecules adhesins, despite very low sequence identity. An extended β-sheet on one side of the compressed, two-sided barrel presents a basic groove that possibly binds to the acidic helical rod domain of KRT10. Our study also demonstrates the importance of the other side of the barrel, formed by extensive well-ordered loops and stabilized by short β-strands, for interaction with KRT10.
External linksOpen Biol / PubMed:24430336 / PubMed Central
MethodsSAS (X-ray synchrotron) / X-ray diffraction
Resolution2 - 2.25 Å
Structure data

SASDAR3:
PsrP functional binding region (Functional binding region (187-385) of the pneumococcal serine-rich repeat protein, PsrP BR(187-385))
Method: SAXS/SANS

PDB-3zgh:
Crystal structure of the KRT10-binding region domain of the pneumococcal serine rich repeat protein PsrP
Method: X-RAY DIFFRACTION / Resolution: 2.0 Å

PDB-3zgi:
Crystal structure of the KRT10-binding region domain of the pneumococcal serine rich repeat protein PsrP
Method: X-RAY DIFFRACTION / Resolution: 2.25 Å

Chemicals

ChemComp-ACT:
ACETATE ION

ChemComp-GOL:
GLYCEROL

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

ChemComp-SO4:
SULFATE ION

Source
  • streptococcus pneumoniae (bacteria)
KeywordsSTRUCTURAL PROTEIN / MSCRAMM / KERATIN-10 / ADHESIN / SRRP

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