EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insights into chromatin remodeling by ISWI during active ATP hydrolysis.
ジャーナル・号・ページ	Science, Page eadu5654, Year 2025
掲載日	2025年4月3日
著者	Youyang Sia / Han Pan / Kangjing Chen / Zhucheng Chen /
PubMed 要旨	Chromatin remodelers utilize the energy of adenosine triphosphate (ATP) hydrolysis to slide nucleosomes, regulating chromatin structure and gene activity in cells. In this work, we report structures ...Chromatin remodelers utilize the energy of adenosine triphosphate (ATP) hydrolysis to slide nucleosomes, regulating chromatin structure and gene activity in cells. In this work, we report structures of imitation switch (ISWI) bound to the nucleosome during active ATP hydrolysis and remodeling, revealing conformational transitions of the remodeling motor across the adenosine triphosphatase (ATPase) cycle. The DNA strands are distorted accordingly, showing one full base-pair bulge and a loss of histone contact at the site of motor binding in the adenosine diphosphate* b and Apo* states. We also identify several important elements for regulation of the remodeling activity. Notably, an enzyme conformation exiting the remodeling cycle reveals a linker DNA-sensing brake mechanism. Together, our findings elucidate a multistate model of ISWI action, providing a comprehensive mechanism of DNA translocation and regulation underpinning chromatin remodeling.
リンク	Science / PubMed:40179160
手法	EM (単粒子)
解像度	2.3 - 3.0 Å
構造データ	61624 9jnp EMDB-61624, PDB-9jnp: Structure of isw1-nucleosome complex in ATP state 手法: EM (単粒子) / 解像度: 2.3 Å 61626 9jnt EMDB-61626, PDB-9jnt: Structure of isw1-nucleosome complex in ADP* state 手法: EM (単粒子) / 解像度: 2.7 Å 61627 9jnu EMDB-61627, PDB-9jnu: Structure of isw1-nucleosome complex in ADP state 手法: EM (単粒子) / 解像度: 2.5 Å 61628 9jnv EMDB-61628, PDB-9jnv: Structure of isw1-nucleosome complex in ADP(S) state 手法: EM (単粒子) / 解像度: 3.0 Å 61629 9jnw EMDB-61629, PDB-9jnw: Structure of isw1-nucleosome complex in ADP+ state 手法: EM (単粒子) / 解像度: 2.9 Å 61630 9jnx EMDB-61630, PDB-9jnx: Structure of isw1-nucleosome complex in *ADP+ state 手法: EM (単粒子) / 解像度: 3.0 Å 61632 9jnz EMDB-61632, PDB-9jnz: Structure of isw1-nucleosome complex in Apo state 手法: EM (単粒子) / 解像度*: 3.0 Å 61641 9jo2 EMDB-61641, PDB-9jo2: Structure of isw1-nucleosome complex in Apo state 手法: EM (単粒子) / 解像度: 3.0 Å 61644 9jo5 EMDB-61644, PDB-9jo5: Structure of isw1-nucleosome complex in ADP-B state 手法: EM (単粒子) / 解像度: 2.8 Å 63123 9liu EMDB-63123, PDB-9liu: Structure of isw1-nucleosome double-bound complex in ATP-ATP state 手法: EM (単粒子) / 解像度: 2.7 Å 63131 9lj2 EMDB-63131, PDB-9lj2: Structure of isw1-nucleosome double-bound complex in ADP-ADP+ state 手法: EM (単粒子) / 解像度: 2.98 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-CL: Unknown entry / クロリド
由来	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母) xenopus laevis (アフリカツメガエル) saccharomyces cerevisiae s288c (パン酵母) escherichia coli k-12 (大腸菌)
キーワード	DNA BINDING PROTEIN/DNA / Chromatin Remodeler / Nucleosome / DNA BINDING PROTEIN-DNA complex / DNA BINDING PROTEIN