EMDB/PDB/SASBDBから引用されている文献のデータベース

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構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
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IF	>30 >20 >10 >5 all

タイトル	Structural basis for synthase activation and cellulose modification in the E. coli Type II Bcs secretion system.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 8799, Year 2024
掲載日	2024年10月11日
著者	Itxaso Anso / Samira Zouhir / Thibault Géry Sana / Petya Violinova Krasteva /
PubMed 要旨	Bacterial cellulosic polymers constitute a prevalent class of biofilm matrix exopolysaccharides that are synthesized by several types of bacterial cellulose secretion (Bcs) systems, which include ...Bacterial cellulosic polymers constitute a prevalent class of biofilm matrix exopolysaccharides that are synthesized by several types of bacterial cellulose secretion (Bcs) systems, which include conserved cyclic diguanylate (c-di-GMP)-dependent cellulose synthase modules together with diverse accessory subunits. In E. coli, the biogenesis of phosphoethanolamine (pEtN)-modified cellulose relies on the BcsRQABEFG macrocomplex, encompassing inner-membrane and cytosolic subunits, and an outer membrane porin, BcsC. Here, we use cryogenic electron microscopy to shed light on the molecular mechanisms of BcsA-dependent recruitment and stabilization of a trimeric BcsG pEtN-transferase for polymer modification, and a dimeric BcsF-dependent recruitment of an otherwise cytosolic BcsERQ regulatory complex. We further demonstrate that BcsE, a secondary c-di-GMP sensor, can remain dinucleotide-bound and retain the essential-for-secretion BcsRQ partners onto the synthase even in the absence of direct c-di-GMP-synthase complexation, likely lowering the threshold for c-di-GMP-dependent synthase activation. Such activation-by-proxy mechanism could allow Bcs secretion system activity even in the absence of substantial intracellular c-di-GMP increase, and is reminiscent of other widespread synthase-dependent polysaccharide secretion systems where dinucleotide sensing and/or synthase stabilization are carried out by key co-polymerase subunits.
リンク	Nat Commun / PubMed:39394223 / PubMed Central
手法	EM (単粒子)
解像度	2.35 - 7.0 Å
構造データ	50567 9fmt EMDB-50567, PDB-9fmt: Cryo-EM structure of the BcsB hexameric crown from the E. coli cellulose secretion macrocomplex 手法: EM (単粒子) / 解像度: 2.35 Å 50571 9fmv EMDB-50571, PDB-9fmv: Cryo-EM structure of the c-di-GMP-free synthase:pEtN transferase complex (BcsA-Bct-G3) from the E. coli cellulose secretion macrocomplex 手法: EM (単粒子) / 解像度: 3.43 Å 50581 9fmz EMDB-50581, PDB-9fmz: Cryo-EM structure of the c-di-GMP-bound synthase:pEtN transferase complex (BcsA-Bct-G3) from the E. coli cellulose secretion macrocomplex 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-50584: Cryo-EM structure of the c-di-GMP-saturated Bcs macrocomplex (BcsABR2Q2E2F2G3) for cellulose secretion of E. coli (filtered to 6A) 手法: EM (単粒子) / 解像度: 6.0 Å EMDB-50595: Cryo-EM structure of the c-di-GMP non-saturated Bcs macrocomplex (BcsABR2Q2E2F2G3) for cellulose secretion of E. coli (filtered to 7A) 手法: EM (単粒子) / 解像度: 7.0 Å 50599 9fnn EMDB-50599, PDB-9fnn: Cryo-EM structure of the c-di-GMP-saturated 'crown'less Bcs macrocomplex for cellulose secretion in E. coli 手法: EM (単粒子) / 解像度: 2.85 Å 50619 9fo7 EMDB-50619, PDB-9fo7: Cryo-EM structure of the BcsE2F2 regulatory subcomplex from the E. coli Bcs macrocomplex for cellulose secretion (local refinement) 手法: EM (単粒子) / 解像度: 2.85 Å 50632 9fp0 EMDB-50632, PDB-9fp0: Cryo-EM structure of the 'crown'less Bcs macrocomplex for E. coli cellulose secretion in non-saturating c-di-GMP (local) 手法: EM (単粒子) / 解像度: 3.37 Å 50633 9fp2 EMDB-50633, PDB-9fp2: Cryo-EM structure of the BcsEFRQ regulatory subcomplex for E. coli cellulose secretion in non-saturating c-di-GMP (local) 手法: EM (単粒子) / 解像度: 3.76 Å
化合物	ChemComp-C2E: 9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one) / c-di-GMP ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	escherichia coli (大腸菌)
キーワード	MEMBRANE PROTEIN / Bacterial cellulose secretion