EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	mTORC1 phosphorylates and stabilizes LST2 to negatively regulate EGFR.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 121, Issue 34, Page e2405959121, Year 2024
掲載日	2024年8月20日
著者	Stefania Battaglioni / Louise-Marie Craigie / Sofia Filippini / Timm Maier / Michael N Hall /
PubMed 要旨	TORC1 (target of rapamycin complex 1) is a highly conserved protein kinase that plays a central role in regulating cell growth. Given the role of mammalian TORC1 (mTORC1) in metabolism and disease, ...TORC1 (target of rapamycin complex 1) is a highly conserved protein kinase that plays a central role in regulating cell growth. Given the role of mammalian TORC1 (mTORC1) in metabolism and disease, understanding mTORC1 downstream signaling and feedback loops is important. mTORC1 recognizes some of its substrates via a five amino acid binding sequence called the TOR signaling (TOS) motif. mTORC1 binding to a TOS motif facilitates phosphorylation of a distinct, distal site. Here, we show that LST2, also known as ZFYVE28, contains a TOS motif (amino acids 401 to 405) and is directly phosphorylated by mTORC1 at serine 670 (S670). mTORC1-mediated S670 phosphorylation promotes LST2 monoubiquitination on lysine 87 (K87). Monoubiquitinated LST2 is stable and displays a broad reticular distribution. When mTORC1 is inactive, unphosphorylated LST2 is degraded by the proteasome. The absence of LST2 enhances EGFR (epidermal growth factor receptor) signaling. We propose that mTORC1 negatively feeds back on its upstream receptor EGFR via LST2.
リンク	Proc Natl Acad Sci U S A / PubMed:39141345 / PubMed Central
手法	EM (単粒子)
解像度	3.27 - 4.02 Å
構造データ	50181 9f42 EMDB-50181, PDB-9f42: cryo-EM structure of LST2 TOS peptide bound to human mTOR complex 1, focused on RAPTOR 手法: EM (単粒子) / 解像度: 3.27 Å 50182 9f43 EMDB-50182, PDB-9f43: cryo-EM structure of human LST2 bound to human mTOR complex 1, focused on RAPTOR 手法: EM (単粒子) / 解像度: 3.49 Å 50183 9f44 EMDB-50183, PDB-9f44: cryo-EM structure of LST2 TOS peptide bound to human mTOR complex 1 手法: EM (単粒子) / 解像度: 3.68 Å 50184 9f45 EMDB-50184, PDB-9f45: cryo-EM structure of human LST2 bound to human mTOR complex 1 手法: EM (単粒子) / 解像度: 3.74 Å EMDB-50253: cryo-EM structure of human LST2 bound to human mTOR complex 1 手法: EM (単粒子) / 解像度: 4.02 Å EMDB-50254: Cryo-EM structure of human LST2 bound to human mTOR complex 1, focused on one protomer 手法: EM (単粒子) / 解像度: 3.75 Å EMDB-50255: Cryo-EM structure of human LST2 bound to human mTOR complex 1, focused on one protomer 手法: EM (単粒子) / 解像度: 3.73 Å
化合物	ChemComp-IHP: INOSITOL HEXAKISPHOSPHATE / フィチン酸
由来	homo sapiens (ヒト)
キーワード	SIGNALING PROTEIN / MTOR / MTORC1 / LST2 / ZFYVE28 / EGFR / TOS