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Yorodumi- EMDB-50182: cryo-EM structure of human LST2 bound to human mTOR complex 1, fo... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-50182 | |||||||||
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Title | cryo-EM structure of human LST2 bound to human mTOR complex 1, focused on RAPTOR | |||||||||
Map data | ||||||||||
Sample |
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Keywords | MTOR / MTORC1 / LST2 / ZFYVE28 / EGFR / TOS / SIGNALING PROTEIN | |||||||||
Function / homology | Function and homology information negative regulation of epidermal growth factor-activated receptor activity / positive regulation of pentose-phosphate shunt / TORC1 complex / TORC1 signaling / positive regulation of odontoblast differentiation / cellular response to L-leucine / Amino acids regulate mTORC1 / MTOR signalling / Energy dependent regulation of mTOR by LKB1-AMPK / phosphatidylinositol-3-phosphate binding ...negative regulation of epidermal growth factor-activated receptor activity / positive regulation of pentose-phosphate shunt / TORC1 complex / TORC1 signaling / positive regulation of odontoblast differentiation / cellular response to L-leucine / Amino acids regulate mTORC1 / MTOR signalling / Energy dependent regulation of mTOR by LKB1-AMPK / phosphatidylinositol-3-phosphate binding / positive regulation of osteoclast differentiation / cellular response to osmotic stress / protein serine/threonine kinase inhibitor activity / enzyme-substrate adaptor activity / positive regulation of transcription by RNA polymerase III / regulation of cell size / Macroautophagy / protein kinase activator activity / negative regulation of epidermal growth factor receptor signaling pathway / mTORC1-mediated signalling / social behavior / HSF1-dependent transactivation / positive regulation of TOR signaling / TOR signaling / positive regulation of G1/S transition of mitotic cell cycle / cellular response to nutrient levels / positive regulation of lipid biosynthetic process / 14-3-3 protein binding / positive regulation of endothelial cell proliferation / cellular response to starvation / positive regulation of glycolytic process / negative regulation of autophagy / positive regulation of peptidyl-threonine phosphorylation / Regulation of PTEN gene transcription / regulation of autophagy / regulation of cell growth / cellular response to amino acid stimulus / TP53 Regulates Metabolic Genes / cellular response to glucose stimulus / small GTPase binding / cytoplasmic stress granule / positive regulation of peptidyl-serine phosphorylation / protein-macromolecule adaptor activity / cellular response to hypoxia / early endosome membrane / positive regulation of cell growth / lysosome / response to xenobiotic stimulus / lysosomal membrane / neuronal cell body / DNA damage response / dendrite / protein-containing complex binding / protein kinase binding / nucleoplasm / metal ion binding / cytosol / cytoplasm Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.49 Å | |||||||||
Authors | Craigie LM / Maier T | |||||||||
Funding support | Switzerland, European Union, 2 items
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Citation | Journal: Proc Natl Acad Sci U S A / Year: 2024 Title: mTORC1 phosphorylates and stabilizes LST2 to negatively regulate EGFR. Authors: Stefania Battaglioni / Louise-Marie Craigie / Sofia Filippini / Timm Maier / Michael N Hall / Abstract: TORC1 (target of rapamycin complex 1) is a highly conserved protein kinase that plays a central role in regulating cell growth. Given the role of mammalian TORC1 (mTORC1) in metabolism and disease, ...TORC1 (target of rapamycin complex 1) is a highly conserved protein kinase that plays a central role in regulating cell growth. Given the role of mammalian TORC1 (mTORC1) in metabolism and disease, understanding mTORC1 downstream signaling and feedback loops is important. mTORC1 recognizes some of its substrates via a five amino acid binding sequence called the TOR signaling (TOS) motif. mTORC1 binding to a TOS motif facilitates phosphorylation of a distinct, distal site. Here, we show that LST2, also known as ZFYVE28, contains a TOS motif (amino acids 401 to 405) and is directly phosphorylated by mTORC1 at serine 670 (S670). mTORC1-mediated S670 phosphorylation promotes LST2 monoubiquitination on lysine 87 (K87). Monoubiquitinated LST2 is stable and displays a broad reticular distribution. When mTORC1 is inactive, unphosphorylated LST2 is degraded by the proteasome. The absence of LST2 enhances EGFR (epidermal growth factor receptor) signaling. We propose that mTORC1 negatively feeds back on its upstream receptor EGFR via LST2. | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_50182.map.gz | 484 MB | EMDB map data format | |
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Header (meta data) | emd-50182-v30.xml emd-50182.xml | 18.8 KB 18.8 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_50182_fsc.xml | 16.9 KB | Display | FSC data file |
Images | emd_50182.png | 76.3 KB | ||
Masks | emd_50182_msk_1.map | 512 MB | Mask map | |
Filedesc metadata | emd-50182.cif.gz | 7.2 KB | ||
Others | emd_50182_half_map_1.map.gz emd_50182_half_map_2.map.gz | 474.6 MB 474.6 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-50182 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-50182 | HTTPS FTP |
-Validation report
Summary document | emd_50182_validation.pdf.gz | 884.6 KB | Display | EMDB validaton report |
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Full document | emd_50182_full_validation.pdf.gz | 884.1 KB | Display | |
Data in XML | emd_50182_validation.xml.gz | 25.6 KB | Display | |
Data in CIF | emd_50182_validation.cif.gz | 33.8 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-50182 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-50182 | HTTPS FTP |
-Related structure data
Related structure data | 9f43MC 9f42C 9f44C 9f45C M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_50182.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.31 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
File | emd_50182_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_50182_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_50182_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : mTORC1 in complex with LST2
Entire | Name: mTORC1 in complex with LST2 |
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Components |
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-Supramolecule #1: mTORC1 in complex with LST2
Supramolecule | Name: mTORC1 in complex with LST2 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: Homo sapiens (human) |
-Macromolecule #1: Regulatory-associated protein of mTOR
Macromolecule | Name: Regulatory-associated protein of mTOR / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 152.764656 KDa |
Recombinant expression | Organism: Spodoptera frugiperda (fall armyworm) |
Sequence | String: HHHHHHHHHH EQKLISEEDL DYKDDDDKME SEMLQSPLLG LGEEDEADLT DWNLPLAFMK KRHCEKIEGS KSLAQSWRMK DRMKTVSVA LVLCLNVGVD PPDVVKTTPC ARLECWIDPL SMGPQKALET IGANLQKQYE NWQPRARYKQ SLDPTVDEVK K LCTSLRRN ...String: HHHHHHHHHH EQKLISEEDL DYKDDDDKME SEMLQSPLLG LGEEDEADLT DWNLPLAFMK KRHCEKIEGS KSLAQSWRMK DRMKTVSVA LVLCLNVGVD PPDVVKTTPC ARLECWIDPL SMGPQKALET IGANLQKQYE NWQPRARYKQ SLDPTVDEVK K LCTSLRRN AKEERVLFHY NGHGVPRPTV NGEVWVFNKN YTQYIPLSIY DLQTWMGSPS IFVYDCSNAG LIVKSFKQFA LQ REQELEV AAINPNHPLA QMPLPPSMKN CIQLAACEAT ELLPMIPDLP ADLFTSCLTT PIKIALRWFC MQKCVSLVPG VTL DLIEKI PGRLNDRRTP LGELNWIFTA ITDTIAWNVL PRDLFQKLFR QDLLVASLFR NFLLAERIMR SYNCTPVSSP RLPP TYMHA MWQAWDLAVD ICLSQLPTII EEGTAFRHSP FFAEQLTAFQ VWLTMGVENR NPPEQLPIVL QVLLSQVHRL RALDL LGRF LDLGPWAVSL ALSVGIFPYV LKLLQSSARE LRPLLVFIWA KILAVDSSCQ ADLVKDNGHK YFLSVLADPY MPAEHR TMT AFILAVIVNS YHTGQEACLQ GNLIAICLEQ LNDPHPLLRQ WVAICLGRIW QNFDSARWCG VRDSAHEKLY SLLSDPI PE VRCAAVFALG TFVGNSAERT DHSTTIDHNV AMMLAQLVSD GSPMVRKELV VALSHLVVQY ESNFCTVALQ FIEEEKNY A LPSPATTEGG SLTPVRDSPC TPRLRSVSSY GNIRAVATAR SLNKSLQNLS LTEESGGAVA FSPGNLSTSS SASSTLGSP ENEEHILSFE TIDKMRRASS YSSLNSLIGV SFNSVYTQIW RVLLHLAADP YPEVSDVAMK VLNSIAYKAT VNARPQRVLD TSSLTQSAP ASPTNKGVHI HQAGGSPPAS STSSSSLTND VAKQPVSRDL PSGRPGTTGP AGAQYTPHSH QFPRTRKMFD K GPEQTADD ADDAAGHKSF ISATVQTGFC DWSARYFAQP VMKIPEEHDL ESQIRKEREW RFLRNSRVRR QAQQVIQKGI TR LDDQIFL NRNPGVPSVV KFHPFTPCIA VADKDSICFW DWEKGEKLDY FHNGNPRYTR VTAMEYLNGQ DCSLLLTATD DGA IRVWKN FADLEKNPEM VTAWQGLSDM LPTTRGAGMV VDWEQETGLL MSSGDVRIVR IWDTDREMKV QDIPTGADSC VTSL SCDSH RSLIVAGLGD GSIRVYDRRM ALSECRVMTY REHTAWVVKA SLQKRPDGHI VSVSVNGDVR IFDPRMPESV NVLQI VKGL TALDIHPQAD LIACGSVNQF TAIYNSSGEL INNIKYYDGF MGQRVGAISC LAFHPHWPHL AVGSNDYYIS VYSVEK RVR UniProtKB: Regulatory-associated protein of mTOR |
-Macromolecule #2: Lateral signaling target protein 2 homolog
Macromolecule | Name: Lateral signaling target protein 2 homolog / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 96.604477 KDa |
Recombinant expression | Organism: Spodoptera frugiperda (fall armyworm) |
Sequence | String: MMNRFRKWLY KPKRSDPQLL ARFYYADEEL NQVAAELDSL DGRKDPQRCT LLVSQFRSCQ DNVLNIINQI MDECIPQDRA PRDFCVKFP EEIRHDNLAG QLWFGAECLA AGSIIMNREL ESMAMRPLAK ELTRSLEDVR GALRDQALRD LNTYTEKMRE A LRHFDVLF ...String: MMNRFRKWLY KPKRSDPQLL ARFYYADEEL NQVAAELDSL DGRKDPQRCT LLVSQFRSCQ DNVLNIINQI MDECIPQDRA PRDFCVKFP EEIRHDNLAG QLWFGAECLA AGSIIMNREL ESMAMRPLAK ELTRSLEDVR GALRDQALRD LNTYTEKMRE A LRHFDVLF AEFELSYVSA MVPVKSPREY YVQQEVIVLF CETVERALDF GYLTQDMIDD YEPALMFSIP RLAIVCGLVV YA DGPLNLD RKVEDMSELF RPFHTLLRKI RDLLQTLTEE ELHTLERNLC ISQDVEFPIR ADVQGPAALA PALSAPLPPE GPL SAKAKD PDAELACSMQ YDDQELEQLS RMVHRAGDEM SSLLSPPIAC QSPAHRPGAE GSPGGEASPG RPRLRSGSDE EERV FFMDD VEGTAEALAR PESPAGPFGW AGSTWADPQE KGQGGPGGAA GISLPASEKE EDLSNNNLEA EGTDGASLAG TSSCS CLDS RLHLDGWEVG ADDAETAEMI AHRTGGMKLS ATVIFNPKSP TSLDSAVATQ EAASEPVAEG MDGGPHKLST GATNCL LHS CVCCGSCGDS REDVVERLRE KCSPGGVIGA SYAAGLAKAS DRAPERQEEA PPPSEDASNG REPKAPTSDK CLPHTSG SQ VDTASGLQGE AGVAGQQEPE ARELHAGSPS AHEAPQALSG SSSSTAGSCS SDKMGPEAAP AATHAAPQAT REKIRSRF H GSHDLIHRLF VCISGVADQL QTNYASDLRS ILKTLFEVMA TKPETDDKEK LRKVTQTLRS AALEDCALCQ ETLSSSELA AKTRDGDFED PPEWVPDEAC GFCTACKAPF TVIRRKHHCR SCGKIFCSRC SSHSAPLPRY GQVKPVRVCT HCYMFHVTPF YSDKAGL UniProtKB: Lateral signaling target protein 2 homolog |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 1 mg/mL |
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Buffer | pH: 8 |
Grid | Model: Quantifoil R2/1 / Material: GOLD / Mesh: 200 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec. / Pretreatment - Atmosphere: AIR |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 281 K / Instrument: FEI VITROBOT MARK IV |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Number grids imaged: 1 / Number real images: 4719 / Average electron dose: 43.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |