EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insights into human topoisomerase 3β DNA and RNA catalysis and nucleic acid gate dynamics.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 834, Year 2025
掲載日	2025年1月19日
著者	Xi Yang / Xuemin Chen / Wei Yang / Yves Pommier /
PubMed 要旨	Type IA topoisomerases (TopoIAs) are present in all living organisms. They resolve DNA/RNA catenanes, knots and supercoils by breaking and rejoining single-stranded DNA/RNA segments and allowing the ...Type IA topoisomerases (TopoIAs) are present in all living organisms. They resolve DNA/RNA catenanes, knots and supercoils by breaking and rejoining single-stranded DNA/RNA segments and allowing the passage of another nucleic acid segment through the break. Topoisomerase III-β (TOP3B), the only RNA topoisomerase in metazoans, promotes R-loop disassembly and translation of mRNAs. Defects in TOP3B lead to severe neurological diseases. We present a series of cryo-EM structures of human TOP3B with its cofactor TDRD3 during cleavage and rejoining of DNA or RNA, thus elucidating the roles of divalent metal ions and key enzyme residues in each step of the catalytic cycle. We also obtained the structure of an open-gate configuration that addresses the long-standing question of the strand-passage mechanism. Our studies reveal how TOP3B catalyzes both DNA and RNA relaxation, while TOP3A acts only on DNA.
リンク	Nat Commun / PubMed:39828754 / PubMed Central
手法	EM (単粒子)
解像度	3.01 - 4.25 Å
構造データ	45374 9c9w EMDB-45374, PDB-9c9w: Dimerized human TOP3B-TDRD3 core complex with a DNA mismatch bubble 手法: EM (単粒子) / 解像度: 4.25 Å 45376 9c9y EMDB-45376, PDB-9c9y: Human TOP3B-TDRD3 core complex in DNA pre-cleavage state 手法: EM (単粒子) / 解像度: 3.35 Å 45378 9ca0 EMDB-45378, PDB-9ca0: Human TOP3B-TDRD3 core complex in DNA post-cleavage state 手法: EM (単粒子) / 解像度: 3.48 Å 45379 9ca1 EMDB-45379, PDB-9ca1: Human TOP3B-TDRD3 core complex in DNA religation state 手法: EM (単粒子) / 解像度: 3.26 Å 45380 9ca4 EMDB-45380, PDB-9ca4: Human TOP3B-TDRD3 core complex in RNA rejoining state 手法: EM (単粒子) / 解像度: 3.01 Å 45390 9cag EMDB-45390, PDB-9cag: Human TOP3B-TDRD3 core complex in RNA pre-cleavage state 手法: EM (単粒子) / 解像度: 3.33 Å 45391 9cah EMDB-45391, PDB-9cah: Human TOP3B-TDRD3 complex 手法: EM (単粒子) / 解像度: 3.16 Å 45393 9caj EMDB-45393, PDB-9caj: Human TOP3B-TDRD3 core complex in pre-cleavage state with ssDNA 5'-ACAGATATT-3 手法: EM (単粒子) / 解像度: 3.51 Å 45394 9cak EMDB-45394, PDB-9cak: Human TOP3B-TDRD3 core complex in DNA rejoining state with ssDNA 5'-GACAGATATT-3 手法: EM (単粒子) / 解像度: 3.01 Å 45395 9cal EMDB-45395, PDB-9cal: Human TOP3B-TDRD3 core complex in post-cleavage state with ssDNA 5'-ACAGATATT-3 手法: EM (単粒子) / 解像度: 3.15 Å
化合物	ChemComp-MN: Unknown entry ChemComp-ZN: Unknown entry
由来	homo sapiens (ヒト)
キーワード	ISOMERASE/DNA / Topoisomerase / DNA / Supercoiling / ISOMERASE-DNA complex / ISOMERASE/RNA / ISOMERASE-RNA complex / ISOMERASE