EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The structural basis of the activation and inhibition of DSR2 NADase by phage proteins.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 6185, Year 2024
掲載日	2024年7月23日
著者	Ruiwen Wang / Qi Xu / Zhuoxi Wu / Jialu Li / Hao Guo / Tianzhui Liao / Yuan Shi / Ling Yuan / Haishan Gao / Rong Yang / Zhubing Shi / Faxiang Li /
PubMed 要旨	DSR2, a Sir2 domain-containing protein, protects bacteria from phage infection by hydrolyzing NAD. The enzymatic activity of DSR2 is triggered by the SPR phage tail tube protein (TTP), while ...DSR2, a Sir2 domain-containing protein, protects bacteria from phage infection by hydrolyzing NAD. The enzymatic activity of DSR2 is triggered by the SPR phage tail tube protein (TTP), while suppressed by the SPbeta phage-encoded DSAD1 protein, enabling phages to evade the host defense. However, the molecular mechanisms of activation and inhibition of DSR2 remain elusive. Here, we report the cryo-EM structures of apo DSR2, DSR2-TTP-NAD and DSR2-DSAD1 complexes. DSR2 assembles into a head-to-head tetramer mediated by its Sir2 domain. The C-terminal helical regions of DSR2 constitute four partner-binding cavities with opened and closed conformation. Two TTP molecules bind to two of the four C-terminal cavities, inducing conformational change of Sir2 domain to activate DSR2. Furthermore, DSAD1 competes with the activator for binding to the C-terminal cavity of DSR2, effectively suppressing its enzymatic activity. Our results provide the mechanistic insights into the DSR2-mediated anti-phage defense system and DSAD1-dependent phage immune evasion.
リンク	Nat Commun / PubMed:39039073 / PubMed Central
手法	EM (単粒子)
解像度	3.11 - 4.14 Å
構造データ	38824 8y13 EMDB-38824, PDB-8y13: Cryo-EM structure of anti-phage defense associated DSR2 tetramer (H171A) 手法: EM (単粒子) / 解像度: 3.18 Å EMDB-38867: The Cryo-EM map of anti-phage defense associated DSR2 tetramer (H171A) 手法: EM (単粒子) / 解像度: 3.18 Å EMDB-38868: Local refinement of anti-phage defense associated DSR2 tetramer (H171A) 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-38869: Local refinement of anti-phage defense associated DSR2 tetramer (H171A) 手法: EM (単粒子) / 解像度: 3.52 Å EMDB-38870: The Cryo-EM map of anti-phage defense associated DSR2 (H171A) before local refinement (map2) 手法: EM (単粒子) / 解像度: 3.11 Å EMDB-38871: Local refinement of anti-phage defense associated DSR2 (H171A) (map2) 手法: EM (単粒子) / 解像度: 3.14 Å 38872 8y34 EMDB-38872, PDB-8y34: Cryo-EM structure of anti-phage defense associated DSR2 (H171A) (map2) 手法: EM (単粒子) / 解像度: 3.11 Å EMDB-38887: The Cryo-EM map of DSR2-DSAD1 complex (cross-linked) before local refinement 手法: EM (単粒子) / 解像度: 3.25 Å EMDB-38888: Local refinement of DSR2-DSAD1 complex (cross-linked) 手法: EM (単粒子) / 解像度: 3.2 Å 38889 8y3m EMDB-38889, PDB-8y3m: Cryo-EM structure of DSR2-DSAD1 complex (cross-linked) 手法: EM (単粒子) / 解像度: 3.25 Å EMDB-38890: The Cryo-EM map of DSR2 tetramer bound with two DSAD1 inhibitor on the same side before local refinement 手法: EM (単粒子) / 解像度: 3.49 Å EMDB-38891: Local refinement of DSR2 tetramer bound with two DSAD1 inhibitor on the same side 手法: EM (単粒子) / 解像度: 3.37 Å EMDB-38901: Local refinement of DSR2 tetramer bound with two DSAD1 inhibitor on the same side 手法: EM (単粒子) / 解像度: 3.39 Å 38902 8y3w EMDB-38902, PDB-8y3w: The Cryo-EM structure of anti-phage defense associated DSR2 tetramer bound with two DSAD1 inhibitors (same side) 手法: EM (単粒子) / 解像度: 3.49 Å 38907 8y3y EMDB-38907, PDB-8y3y: The Cryo-EM structure of anti-phage defense associated DSR2 tetramer bound with two DSAD1 inhibitors (opposite side) 手法: EM (単粒子) / 解像度: 3.33 Å EMDB-39912: The cryo-EM map of DSR2-tail tube-NAD complex before local refinement 手法: EM (単粒子) / 解像度: 4.14 Å EMDB-39913: Local refinement of DSR2-tail tube-NAD+ complex 手法: EM (単粒子) / 解像度: 3.17 Å EMDB-39914: Local refinement of DSR2-tail tube-NAD+ complex 手法: EM (単粒子) / 解像度: 3.4 Å 39925 8zc9 EMDB-39925, PDB-8zc9: The Cryo-EM structure of DSR2-Tail tube-NAD+ complex 手法: EM (単粒子) / 解像度: 3.14 Å
化合物	ChemComp-NAD: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM
由来	bacillus subtilis (枯草菌) Bacillus subtilis
キーワード	IMMUNE SYSTEM / NADase / anti-phage defense / tetramer / IMMUNOSUPPRESSANT / complex / immune evasion / tail-tube protein / NAD