EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Signaling by a bacterial phytochrome histidine kinase involves a conformational cascade reorganizing the dimeric photoreceptor.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 6853, Year 2024
掲載日	2024年8月10日
著者	E Sethe Burgie / Katherine Basore / Michael J Rau / Brock Summers / Alayna J Mickles / Vadim Grigura / James A J Fitzpatrick / Richard D Vierstra /
PubMed 要旨	Phytochromes (Phys) are a divergent cohort of bili-proteins that detect light through reversible interconversion between dark-adapted Pr and photoactivated Pfr states. While our understandings of ...Phytochromes (Phys) are a divergent cohort of bili-proteins that detect light through reversible interconversion between dark-adapted Pr and photoactivated Pfr states. While our understandings of downstream events are emerging, it remains unclear how Phys translate light into an interpretable conformational signal. Here, we present models of both states for a dimeric Phy with histidine kinase (HK) activity from the proteobacterium Pseudomonas syringae, which were built from high-resolution cryo-EM maps (2.8-3.4-Å) of the photosensory module (PSM) and its following signaling (S) helix together with lower resolution maps for the downstream output region augmented by RoseTTAFold and AlphaFold structural predictions. The head-to-head models reveal the PSM and its photointerconversion mechanism with strong clarity, while the HK region is interpretable but relatively mobile. Pr/Pfr comparisons show that bilin phototransformation alters PSM architecture culminating in a scissoring motion of the paired S-helices linking the PSMs to the HK bidomains that ends in reorientation of the paired catalytic ATPase modules relative to the phosphoacceptor histidines. This action apparently primes autophosphorylation enroute to phosphotransfer to the cognate DNA-binding response regulator AlgB which drives quorum-sensing behavior through transient association with the photoreceptor. Collectively, these models illustrate how light absorption conformationally translates into accelerated signaling by Phy-type kinases.
リンク	Nat Commun / PubMed:39127720 / PubMed Central
手法	EM (単粒子)
解像度	2.81 - 3.3 Å
構造データ	41903 8u4x EMDB-41903, PDB-8u4x: Cryo-EM structure of PsBphP in Pr state 手法: EM (単粒子) / 解像度: 2.81 Å 41941 8u62 EMDB-41941, PDB-8u62: Cryo-EM structure of PsBphP in Pfr state, Dimer of Dimers FL 手法: EM (単粒子) / 解像度: 3.3 Å 41942 8u63 EMDB-41942, PDB-8u63: Cryo-EM structure of PsBphP in Pfr state, Dimer of Dimers PSM only 手法: EM (単粒子) / 解像度: 3.01 Å 41943 8u64 EMDB-41943, PDB-8u64: Cryo-EM structure of PsBphP in Pfr state, medial PSM only 手法: EM (単粒子) / 解像度: 3.13 Å 41944 8u65 EMDB-41944, PDB-8u65: Cryo-EM structure of PsBphP in Pfr state, splayed PSM only 手法: EM (単粒子) / 解像度: 3.01 Å 42030 8u8z EMDB-42030, PDB-8u8z: Cryo-EM structure of PsBphP in Pr state, extended DHp 手法: EM (単粒子) / 解像度: 3.25 Å
化合物	ChemComp-LBV: 3-[2-[(Z)-[3-(2-carboxyethyl)-5-[(Z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium
由来	pseudomonas syringae pv. tomato str. dc3000 (バクテリア)
キーワード	PLANT PROTEIN / Pseudomonas syringae bacteriophytochrome