EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	BtuB TonB-dependent transporters and BtuG surface lipoproteins form stable complexes for vitamin B uptake in gut Bacteroides.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 4714, Year 2023
掲載日	2023年8月5日
著者	Javier Abellon-Ruiz / Kalyanashis Jana / Augustinas Silale / Andrew M Frey / Arnaud Baslé / Matthias Trost / Ulrich Kleinekathöfer / Bert van den Berg /
PubMed 要旨	Vitamin B (cobalamin) is required for most human gut microbes, many of which are dependent on scavenging to obtain this vitamin. Since bacterial densities in the gut are extremely high, competition ...Vitamin B (cobalamin) is required for most human gut microbes, many of which are dependent on scavenging to obtain this vitamin. Since bacterial densities in the gut are extremely high, competition for this keystone micronutrient is severe. Contrasting with Enterobacteria, members of the dominant genus Bacteroides often encode several BtuB vitamin B outer membrane transporters together with a conserved array of surface-exposed B-binding lipoproteins. Here we show that the BtuB transporters from Bacteroides thetaiotaomicron form stable, pedal bin-like complexes with surface-exposed BtuG lipoprotein lids, which bind B with high affinities. Closing of the BtuG lid following B capture causes destabilisation of the bound B by a conserved BtuB extracellular loop, causing translocation of the vitamin to BtuB and subsequent transport. We propose that TonB-dependent, lipoprotein-assisted small molecule uptake is a general feature of Bacteroides spp. that is important for the success of this genus in colonising the human gut.
リンク	Nat Commun / PubMed:37543597 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.33 - 3.72 Å
構造データ	16114 8blw EMDB-16114, PDB-8blw: Vitamin B12 transporter BtuB1 with lipoprotein BtuG1 from B. theta 手法: EM (単粒子) / 解像度: 3.22 Å 17574 8p97 EMDB-17574, PDB-8p97: BtuB3G3 bound to cyanocobalamin with disordered EL8 手法: EM (単粒子) / 解像度: 2.75 Å 17575 8p98 EMDB-17575, PDB-8p98: BtuB3G3 bound to cyanocobalamin with ordered EL8 手法: EM (単粒子) / 解像度: 2.97 Å PDB-8bmx: Bacteroides thetaiotaomicron B12 TonB dependent transporter in complex with a surface lipoprotein 手法: X-RAY DIFFRACTION / 解像度: 3.72 Å PDB-8bmy: Bacteroides thetaiotaomicron surface lipoprotein bound to cyanocobalamin 手法: X-RAY DIFFRACTION / 解像度: 1.72 Å PDB-8bmz: Bacteroides thetaiotaomicron surface lipoprotein BT1954 bound to adenosylcobalamin 手法: X-RAY DIFFRACTION / 解像度: 2.3 Å PDB-8bn0: Bacteroides thetaiotaomicron surface protein BT1954 bound to 手法: X-RAY DIFFRACTION / 解像度: 1.33 Å PDB-8okv: lipoprotein BT2095 from Bacteroides thetaiotamicron bound to cyanocobalamin CnCbl 手法: X-RAY DIFFRACTION / 解像度: 2.6 Å
化合物	ChemComp-GAL: beta-D-galactopyranose / β-D-ガラクトピラノ-ス ChemComp-CNC: CYANOCOBALAMIN ChemComp-CL: Unknown entry / クロリド ChemComp-SO4: SULFATE ION / 硫酸ジアニオン ChemComp-HOH: WATER ChemComp-B1Z: Adenosylcobalamin / 薬剤YM ChemComp-CYN: CYANIDE ION / シアニド ChemComp-CBY*: COB(II)INAMIDE
由来	bacteroides thetaiotaomicron vpi-5482 (バクテリア)
キーワード	MEMBRANE PROTEIN / Outer membrane / TonB-dependent transporter / vitamin B12 / lipoprotein / Vitamin B12 Transport Complex Outer membrane / beta propeller surface protein B12 binding protein / Beta propeller Vitamin B12 binding Surface protein / Surface exposed protein Beta propeller Vitamin B12 binding protein / membrane associated / beta propeller / B12 binding protein / Cyanocobalamin / transporter / complex