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-Structure paper
タイトル | Integrated cryoEM structure of a spumaretrovirus reveals cross-kingdom evolutionary relationships and the molecular basis for assembly and virus entry. |
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ジャーナル・号・ページ | Cell, Vol. 187, Issue 16, Page 4213-4230.e19, Year 2024 |
掲載日 | 2024年8月8日 |
著者 | Thomas Calcraft / Nicole Stanke-Scheffler / Andrea Nans / Dirk Lindemann / Ian A Taylor / Peter B Rosenthal / |
PubMed 要旨 | Foamy viruses (FVs) are an ancient lineage of retroviruses, with an evolutionary history spanning over 450 million years. Vector systems based on Prototype Foamy Virus (PFV) are promising candidates ...Foamy viruses (FVs) are an ancient lineage of retroviruses, with an evolutionary history spanning over 450 million years. Vector systems based on Prototype Foamy Virus (PFV) are promising candidates for gene and oncolytic therapies. Structural studies of PFV contribute to the understanding of the mechanisms of FV replication, cell entry and infection, and retroviral evolution. Here we combine cryoEM and cryoET to determine high-resolution in situ structures of the PFV icosahedral capsid (CA) and envelope glycoprotein (Env), including its type III transmembrane anchor and membrane-proximal external region (MPER), and show how they are organized in an integrated structure of assembled PFV particles. The atomic models reveal an ancient retroviral capsid architecture and an unexpected relationship between Env and other class 1 fusion proteins of the Mononegavirales. Our results represent the de novo structure determination of an assembled retrovirus particle. |
リンク | Cell / PubMed:39013471 |
手法 | EM (単粒子) / EM (サブトモグラム平均) / EM (トモグラフィー) |
解像度 | 2.91 - 13.5 Å |
構造データ | EMDB-17309, PDB-8ozh: EMDB-17311, PDB-8ozj: EMDB-17312, PDB-8ozk: EMDB-17313, PDB-8ozl: EMDB-17314, PDB-8ozm: EMDB-17315, PDB-8ozn: EMDB-17316: In situ subtomogram average of Prototype Foamy Virus Env trimer EMDB-17317, PDB-8ozp: EMDB-17318, PDB-8ozq: EMDB-17319: In situ subtomogram average of the Prototype Foamy Virus capsid, wild-type Gag EMDB-17320: In situ subtomogram average of the Prototype Foamy Virus capsid, p68 Gag EMDB-17321: Cryotomogram of Prototype Foamy Virus particles, wild-type Gag EMDB-17322: Cryotomogram of Prototype Foamy Virus particles, p68 Gag |
化合物 | ChemComp-NAG: ChemComp-CLR: ChemComp-PTY: |
由来 |
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キーワード | VIRAL PROTEIN / membrane fusion / envelope glycoprotein / foamy virus / MPER / transmembrane / MEMBRANE PROTEIN / capsid / Gag / pentamer / hexamer |