EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Hexasome-INO80 complex reveals structural basis of noncanonical nucleosome remodeling.
ジャーナル・号・ページ	Science, Vol. 381, Issue 6655, Page 313-319, Year 2023
掲載日	2023年7月21日
著者	Min Zhang / Anna Jungblut / Franziska Kunert / Luis Hauptmann / Thomas Hoffmann / Olga Kolesnikova / Felix Metzner / Manuela Moldt / Felix Weis / Frank DiMaio / Karl-Peter Hopfner / Sebastian Eustermann /
PubMed 要旨	Loss of H2A-H2B histone dimers is a hallmark of actively transcribed genes, but how the cellular machinery functions in the context of noncanonical nucleosomal particles remains largely elusive. In ...Loss of H2A-H2B histone dimers is a hallmark of actively transcribed genes, but how the cellular machinery functions in the context of noncanonical nucleosomal particles remains largely elusive. In this work, we report the structural mechanism for adenosine 5'-triphosphate-dependent chromatin remodeling of hexasomes by the INO80 complex. We show how INO80 recognizes noncanonical DNA and histone features of hexasomes that emerge from the loss of H2A-H2B. A large structural rearrangement switches the catalytic core of INO80 into a distinct, spin-rotated mode of remodeling while its nuclear actin module remains tethered to long stretches of unwrapped linker DNA. Direct sensing of an exposed H3-H4 histone interface activates INO80, independently of the H2A-H2B acidic patch. Our findings reveal how the loss of H2A-H2B grants remodelers access to a different, yet unexplored layer of energy-driven chromatin regulation.
リンク	Science / PubMed:37384673
手法	EM (単粒子)
解像度	2.7 - 7.59 Å
構造データ	17006 8oo7 EMDB-17006: CryoEM Structure INO80core Hexasome complex composite map state1 PDB-8oo7: CryoEM Structure INO80core Hexasome complex composite model state1 手法: EM (単粒子) / 解像度: 2.8 Å 17007 8oo9 EMDB-17007, PDB-8oo9: CryoEM Structure INO80core Hexasome complex ATPase-DNA refinement state1 手法: EM (単粒子) / 解像度: 3.2 Å 17008 8ooa EMDB-17008, PDB-8ooa: CryoEM Structure INO80core Hexasome complex Hexasome refinement state1 手法: EM (単粒子) / 解像度: 3.18 Å 17010 8ooc EMDB-17010, PDB-8ooc: CryoEM Structure INO80core Hexasome complex Rvb core refinement state1 手法: EM (単粒子) / 解像度: 2.93 Å 17012 8oof EMDB-17012, PDB-8oof: CryoEM Structure INO80core Hexasome complex Arp5 Ies6 refinement state1 手法: EM (単粒子) / 解像度: 2.9 Å 17017 8ook EMDB-17017, PDB-8ook: CryoEM Structure INO80core Hexasome complex Arp5 grappler refinement state1 手法: EM (単粒子) / 解像度: 5.69 Å 17025 8oop EMDB-17025: INO80 core bound to hexasome composite map of state 2 PDB-8oop: CryoEM Structure INO80core Hexasome complex composite model state2 手法: EM (単粒子) / 解像度: 2.7 Å 17026 8oor EMDB-17026, PDB-8oor: CryoEM Structure INO80core Hexasome complex Rvb core refinement state2 手法: EM (単粒子) / 解像度: 2.87 Å 17027 8oos EMDB-17027, PDB-8oos: CryoEM Structure INO80core Hexasome complex ATPase-hexasome refinement state 2 手法: EM (単粒子) / 解像度: 3.29 Å 17028 8oot EMDB-17028, PDB-8oot: CryoEM Structure INO80core Hexasome complex Arp5 Ies6 refinement state2 手法: EM (単粒子) / 解像度: 2.85 Å EMDB-17676: INO80 core bound to hexasome focused refinement of Arp5 grappler 手法: EM (単粒子) / 解像度: 7.59 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-ALF: TETRAFLUOROALUMINATE ION / テトラフルオロアルミナ-ト ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM
由来	thermochaetoides thermophila (菌類) homo sapiens (ヒト) synthetic construct (人工物)
キーワード	DNA BINDING PROTEIN / ATP-dependent chromatin remodeler