EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Precisely patterned nanofibres made from extendable protein multiplexes.
ジャーナル・号・ページ	Nat Chem, Vol. 15, Issue 12, Page 1664-1671, Year 2023
掲載日	2023年9月4日
著者	Neville P Bethel / Andrew J Borst / Fabio Parmeggiani / Matthew J Bick / T J Brunette / Hannah Nguyen / Alex Kang / Asim K Bera / Lauren Carter / Marcos C Miranda / Ryan D Kibler / Mila Lamb / Xinting Li / Banumathi Sankaran / David Baker /
PubMed 要旨	Molecular systems with coincident cyclic and superhelical symmetry axes have considerable advantages for materials design as they can be readily lengthened or shortened by changing the length of the ...Molecular systems with coincident cyclic and superhelical symmetry axes have considerable advantages for materials design as they can be readily lengthened or shortened by changing the length of the constituent monomers. Among proteins, alpha-helical coiled coils have such symmetric, extendable architectures, but are limited by the relatively fixed geometry and flexibility of the helical protomers. Here we describe a systematic approach to generating modular and rigid repeat protein oligomers with coincident C to C and superhelical symmetry axes that can be readily extended by repeat propagation. From these building blocks, we demonstrate that a wide range of unbounded fibres can be systematically designed by introducing hydrophilic surface patches that force staggering of the monomers; the geometry of such fibres can be precisely tuned by varying the number of repeat units in the monomer and the placement of the hydrophilic patches.
リンク	Nat Chem / PubMed:37667012 / PubMed Central
手法	EM (単粒子) / EM (らせん対称) / X線回折
解像度	1.59 - 7.62 Å
構造データ	EMDB-29680: C6HR1_8r: Extendable repeat protein heptamer 手法: EM (単粒子) / 解像度: 4.18 Å EMDB-29847: C3HR3_8r: Extendable repeat protein trimer 手法: EM (単粒子) / 解像度: 5.58 Å 29849 8gaa EMDB-29849, PDB-8gaa: C6HR1_4r: Extendable repeat protein hexamer 手法: EM (単粒子) / 解像度: 4.24 Å EMDB-29851: C4HR1_8r_shift5: Extendable repeat protein fiber 手法: EM (らせん対称) / 解像度: 7.62 Å 29856 8g8i EMDB-29856, PDB-8g8i: C3HR3_9r_shift4: Extendable repeat protein fiber 手法: EM (らせん対称) / 解像度: 3.92 Å 29894 8ga9 EMDB-29894, PDB-8ga9: C4HR1_4r: Extendable repeat protein tetramer 手法: EM (単粒子) / 解像度: 3.71 Å 29904 8gaq EMDB-29904, PDB-8gaq: C5HR2_4r: Extendable repeat protein pentamer 手法: EM (単粒子) / 解像度: 3.73 Å PDB-8eov: Precisely patterned nanofibers made from extendable protein multiplexes 手法: X-RAY DIFFRACTION / 解像度: 1.59 Å PDB-8eox: Precisely patterned nanofibers made from extendable protein multiplexes 手法: X-RAY DIFFRACTION / 解像度: 3.3 Å PDB-8eoz: Precisely patterned nanofibers made from extendable protein multiplexes 手法: X-RAY DIFFRACTION / 解像度: 3 Å PDB-8erw: Precisely patterned nanofibers made from extendable protein multiplexes 手法: X-RAY DIFFRACTION / 解像度: 2.88 Å
化合物	ChemComp-PO4: PHOSPHATE ION / ホスファ-ト ChemComp-HOH: WATER
由来	unidentified (未定義) synthetic construct (人工物)
キーワード	DE NOVO PROTEIN / de novo design / nanofibers / Oligomer / repeat protein