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-Structure paper
タイトル | Principles of human pre-60 biogenesis. |
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ジャーナル・号・ページ | Science, Vol. 381, Issue 6653, Page eadh3892, Year 2023 |
掲載日 | 2023年7月7日 |
著者 | Arnaud Vanden Broeck / Sebastian Klinge / |
PubMed 要旨 | During the early stages of human large ribosomal subunit (60) biogenesis, an ensemble of assembly factors establishes and fine-tunes the essential RNA functional centers of pre-60 particles by an ...During the early stages of human large ribosomal subunit (60) biogenesis, an ensemble of assembly factors establishes and fine-tunes the essential RNA functional centers of pre-60 particles by an unknown mechanism. Here, we report a series of cryo-electron microscopy structures of human nucleolar and nuclear pre-60 assembly intermediates at resolutions of 2.5 to 3.2 angstroms. These structures show how protein interaction hubs tether assembly factor complexes to nucleolar particles and how guanosine triphosphatases and adenosine triphosphatase couple irreversible nucleotide hydrolysis steps to the installation of functional centers. Nuclear stages highlight how a conserved RNA-processing complex, the rixosome, couples large-scale RNA conformational changes with pre-ribosomal RNA processing by the RNA degradation machinery. Our ensemble of human pre-60 particles provides a rich foundation with which to elucidate the molecular principles of ribosome formation. |
リンク | Science / PubMed:37410842 |
手法 | EM (単粒子) |
解像度 | 2.33 - 3.75 Å |
構造データ | EMDB-29104: Human nucleolar pre-60S ribosomal subunit (State A1) - Overall map EMDB-29105: Human nucleolar pre-60S ribosomal subunit (State A2) - Overall map EMDB-29106: Human nucleolar pre-60S ribosomal subunit (State B1) - Overall map EMDB-29107: Human nucleolar pre-60S ribosomal subunit (State B2) - Overall map EMDB-29108: Human nucleolar pre-60S ribosomal subunit (State C1) - Overall map EMDB-29109: Human nucleolar pre-60S ribosomal subunit (State C2) - Overall map EMDB-29110: Human nucleolar pre-60S ribosomal subunit (State D1) - Overall map EMDB-29111: Human nucleolar pre-60S ribosomal subunit (State D2) - Overall map EMDB-29112: Human nucleolar pre-60S ribosomal subunit (State E) - Overall map EMDB-29113: Human nucleolar pre-60S ribosomal subunit (State F) - Overall map EMDB-29114: Human nucleolar pre-60S ribosomal subunit (State G) - Overall map EMDB-29115: Human nucleolar pre-60S ribosomal subunit (State H) - Overall map EMDB-29116: Human nuclear pre-60S ribosomal subunit (State I1) - Overall map EMDB-29117: Human nuclear pre-60S ribosomal subunit (State I2) - Overall map EMDB-29118: Human nuclear pre-60S ribosomal subunit (State I3) - Overall map EMDB-29119: Human nuclear pre-60S ribosomal subunit (State J1) - Overall map EMDB-29120: Human nuclear pre-60S ribosomal subunit (State J2) - Overall map EMDB-29121: Human nuclear pre-60S ribosomal subunit (State J3) - Overall map EMDB-29122: Human nuclear pre-60S ribosomal subunit (State K1) - Overall map EMDB-29123: Human nuclear pre-60S ribosomal subunit (State K2) - Overall map EMDB-29124: Human nuclear pre-60S ribosomal subunit (State K3) - Overall map EMDB-29125: Human nuclear pre-60S ribosomal subunit (State L1) - Overall map EMDB-29126: Human nuclear pre-60S ribosomal subunit (State L2) - Overall map EMDB-29127: Human nuclear pre-60S ribosomal subunit (State L3) - Overall map EMDB-29128: Human nucleolar pre-60S ribosomal subunit (States A1,A2 & B1,B2 combined) - Domain VI root helix focused map EMDB-29129: Human nucleolar pre-60S ribosomal subunit (States A1,A2 & B1,B2 combined) - Domain VI helix 96 focused map EMDB-29130: Human nucleolar pre-60S ribosomal subunit (State A1) - Domain I focused map EMDB-29131: Human nucleolar pre-60S ribosomal subunit (State A1) - Domain II focused map EMDB-29132: Human nucleolar pre-60S ribosomal subunit (State A2) - Domain I focused map EMDB-29133: Human nucleolar pre-60S ribosomal subunit (State A2) - Domain II focused map EMDB-29134: Human nucleolar pre-60S ribosomal subunit (States A1,A2 combined) - Domain VI focused map EMDB-29135: Human nucleolar pre-60S ribosomal subunit (States A1,A2 combined) - ITS2 module focused map EMDB-29136: Human nucleolar pre-60S ribosomal subunit (State B1) - Domain I focused map EMDB-29137: Human nucleolar pre-60S ribosomal subunit (State B1) - Domain II focused map EMDB-29138: Human nucleolar pre-60S ribosomal subunit (State B2) - Domain I focused map EMDB-29139: Human nucleolar pre-60S ribosomal subunit (State B2) - Domain II focused map EMDB-29140: Human nucleolar pre-60S ribosomal subunit (States B1,B2 combined) - ITS2 module focused map EMDB-29141: Human nucleolar pre-60S ribosomal subunit (States B1,B2 combined) - Domain VI focused map EMDB-29142: Human nucleolar pre-60S ribosomal subunit (States B1,B2 combined) - Domain V & GTPB4 focused map EMDB-29143: Human nucleolar pre-60S ribosomal subunit (State C1) - Domain I focused map EMDB-29144: Human nucleolar pre-60S ribosomal subunit (State C1) - Domain II focused map EMDB-29145: Human nucleolar pre-60S ribosomal subunit (State C2) - Domain I focused map EMDB-29146: Human nucleolar pre-60S ribosomal subunit (State C2) - Domain II focused map EMDB-29147: Human nucleolar pre-60S ribosomal subunit (States C1,C2 combined) - ITS2 module focused map EMDB-29148: Human nucleolar pre-60S ribosomal subunit (States C1,C2 combined) - Domains V-VI focused map EMDB-29149: Human nucleolar pre-60S ribosomal subunit (States C1,C2 combined) - DDX54 focused map EMDB-29150: Human nucleolar pre-60S ribosomal subunit (States C1,C2 combined) - L1 stalk focused map EMDB-29151: Human nucleolar pre-60S ribosomal subunit (States A-F combined) - uL15 focused map EMDB-29152: Human nucleolar pre-60S ribosomal subunit (State D1) - Domain I focused map EMDB-29153: Human nucleolar pre-60S ribosomal subunit (State D1), Domain II focused map EMDB-29154: Human nucleolar pre-60S ribosomal subunit (State D1) - Domains V-VI focused map EMDB-29155: Human nucleolar pre-60S ribosomal subunit (State D1) - GNL3 focused map EMDB-29156: Human nucleolar pre-60S ribosomal subunit (State D2) - Domain I focused map EMDB-29157: Human nucleolar pre-60S ribosomal subunit (State D2) - Domain II focused map EMDB-29158: Human nucleolar pre-60S ribosomal subunit (State D2) - Domains V-VI focused map EMDB-29159: Human nucleolar pre-60S ribosomal subunit (States D1,D2 combined) - RRS1 focused map EMDB-29160: Human nucleolar pre-60S ribosomal subunit (States D1,D2 combined) - ITS2 module focused map EMDB-29161: Human nucleolar pre-60S ribosomal subunit (States D1,D2 combined) - Inter-subunit side focused map EMDB-29162: Human nucleolar pre-60S ribosomal subunit (States D1,D2 combined) - DDX54 focused map EMDB-29163: Human nucleolar pre-60S ribosomal subunit (States D1,D2 combined) - L1 stalk focused map EMDB-29164: Human nucleolar pre-60S ribosomal subunit (States D1,D2 combined) - NOC3L & FTSJ3 focused map EMDB-29165: Human nucleolar pre-60S ribosomal subunit (States D1,D2 combined) - NOC2L focused map EMDB-29166: Human nucleolar pre-60S ribosomal subunit (State E) - Domain I focused map EMDB-29167: Human nucleolar pre-60S ribosomal subunit (State E) - Domain II focused map EMDB-29168: Human nucleolar pre-60S ribosomal subunit (State E) - ITS2 module focused map EMDB-29169: Human nucleolar pre-60S ribosomal subunit (State F) - PeBoW-WDR55 focused map EMDB-29170: Human nucleolar pre-60S ribosomal subunit (State F) - PeBoW complex focused map EMDB-29171: Human nucleolar pre-60S ribosomal subunit (State F) - WDR55 focused map EMDB-29173: Human nucleolar pre-60S ribosomal subunit (State F) - Domain III focused map EMDB-29174: Human nucleolar pre-60S ribosomal subunit (State F) - ITS2 module focused map EMDB-29175: Human nucleolar pre-60S ribosomal subunit (State F) - Domain I focused map EMDB-29176: Human nucleolar pre-60S ribosomal subunit (State F) - Domain II focused map EMDB-29177: Human nucleolar pre-60S ribosomal subunit (States E,F combined) - Inter-subunit side focused map EMDB-29178: Human nucleolar pre-60S ribosomal subunit (States E,F combined) - DDX54 focused map EMDB-29179: Human nucleolar pre-60S ribosomal subunit (States E,F combined) - L1 stalk focused map EMDB-29180: Human nucleolar pre-60S ribosomal subunit (States E,F combined) - NOC3L focused map EMDB-29181: Human nucleolar pre-60S ribosomal subunit (States E,F combined) - Domain VI focused map EMDB-29182: Human nucleolar pre-60S ribosomal subunit (States E,F combined) - DDX18 focused map EMDB-29183: Human nucleolar pre-60S ribosomal subunit (States E,F combined) - Domain V focused map EMDB-29184: Human nucleolar pre-60S ribosomal subunit (States E,F combined) - RRS1 focused map EMDB-29185: Human nucleolar pre-60S ribosomal subunit (States E,F combined) - NOC2L focused map EMDB-29186: Human nucleolar pre-60S ribosomal subunit (State G) - ITS2 module focused map EMDB-29187: Human nucleolar pre-60S ribosomal subunit (State G) - Domain I focused map EMDB-29188: Human nucleolar pre-60S ribosomal subunit (State G) - Domain II focused map EMDB-29189: Human nucleolar pre-60S ribosomal subunit (State G) - Domains V-VI focused map EMDB-29192: Human nucleolar pre-60S ribosomal subunit (State G) - Domain IV focused map EMDB-29193: Human nucleolar pre-60S ribosomal subunit (State H) - 5S RNP focused map EMDB-29194: Human nucleolar pre-60S ribosomal subunit (State H) - Domains V-VI focused map EMDB-29195: Human nuclear pre-60S ribosomal subunit (States I1,I2,I3 combined) - Central protuberance focused map EMDB-29196: Human nuclear pre-60S ribosomal subunit (States I1,I2,I3 combined) - TEX10 focused map EMDB-29197: Human nuclear pre-60S ribosomal subunit (States I1,I2,I3 combined) - Rix1 complex focused map EMDB-29198: Human nuclear pre-60S ribosomal subunit (States I1,J1,K1,L1 combined) - ITS2 module focused map EMDB-29199: Human nuclear pre-60S ribosomal subunit (States I2,J2,K2,L2 combined) - ITS2 module focused map EMDB-29200: Human nuclear pre-60S ribosomal subunit (States J1,J2,J3 combined) - Central protuberance focused map EMDB-29201: Human nuclear pre-60S ribosomal subunit (States J1,J2,J3 combined) - Domain V focused map EMDB-29202: Human nuclear pre-60S ribosomal subunit (States K1,K2,K3 combined) - Central protuberance focused map EMDB-29204: Human nuclear pre-60S ribosomal subunit (States K1,K2,K3 combined) - Domain V focused map EMDB-29205: Human nuclear pre-60S ribosomal subunit (States L1,L2,L3 combined) - Central protuberance focused map EMDB-29206: Human nuclear pre-60S ribosomal subunit (States L1,L2,L3 combined) - Domain V focused map EMDB-29252: Human nucleolar pre-60S ribosomal subunit (State A1) - Composite map EMDB-29253: Human nucleolar pre-60S ribosomal subunit (State A2) - Composite map EMDB-29254: Human nucleolar pre-60S ribosomal subunit (State B1) - Composite map EMDB-29255: Human nucleolar pre-60S ribosomal subunit (State B2) - Composite map EMDB-29256: Human nucleolar pre-60S ribosomal subunit (State C1) - Composite map EMDB-29257: Human nucleolar pre-60S ribosomal subunit (State C2) - Composite map EMDB-29258: Human nucleolar pre-60S ribosomal subunit (State D1) - Composite map EMDB-29259: Human nucleolar pre-60S ribosomal subunit (State D2) - Composite map EMDB-29260: Human nucleolar pre-60S ribosomal subunit (State E) - Composite map EMDB-29261: Human nucleolar pre-60S ribosomal subunit (State F) - Composite map EMDB-29262: Human nucleolar pre-60S ribosomal subunit (State G) - Composite map EMDB-29263: Human nucleolar pre-60S ribosomal subunit (State H) - Composite map EMDB-29265: Human nuclear pre-60S ribosomal subunit (State I1) - Composite map EMDB-29266: Human nuclear pre-60S ribosomal subunit (State I2) - Composite map EMDB-29267: Human nuclear pre-60S ribosomal subunit (State I3) - Composite map EMDB-29268: Human nuclear pre-60S ribosomal subunit (State J1) - Composite map EMDB-29269: Human nuclear pre-60S ribosomal subunit (State J2) - Composite map EMDB-29271: Human nuclear pre-60S ribosomal subunit (State J3) - Composite map EMDB-29272: Human nuclear pre-60S ribosomal subunit (State K1) - Composite map EMDB-29273: Human nuclear pre-60S ribosomal subunit (State K2) - Composite map EMDB-29274: Human nuclear pre-60S ribosomal subunit (State K3) - Composite map EMDB-29275: Human nuclear pre-60S ribosomal subunit (State L1) - Composite map EMDB-29276: Human nuclear pre-60S ribosomal subunit (State L2) - Composite map EMDB-29277: Human nuclear pre-60S ribosomal subunit (State L3) - Composite map |
化合物 | ChemComp-MG: ChemComp-ZN: ChemComp-SF4: ChemComp-GDP: ChemComp-K: ChemComp-ADP: ChemComp-GTP: |
由来 |
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キーワード | RIBOSOME / Pre-60S ribosomal subunit / Assembly intermediate / Nucleoprotein complex |