EMDB-29263: Human nucleolar pre-60S ribosomal subunit (State H) - Composite map

Basic information

Entry

Database: EMDB / ID: EMD-29263

• Title: Human nucleolar pre-60S ribosomal subunit (State H) - Composite map

Sample

• Complex: Human nucleolar pre-60S ribosomal subunit (State H)
  • Protein or peptide: x 29 types
  • RNA: x 2 types
• Ligand: x 5 types

• Keywords: Pre-60S ribosomal subunit / Assembly intermediate / Ribosome / Nucleoprotein complex

Function / homology

Function:

mitotic nuclear division / positive regulation of protein localization to chromosome, telomeric region / : / dendrite extension / lamin filament / regulation of Notch signaling pathway / preribosome binding / regulation of megakaryocyte differentiation / regulation of fatty acid biosynthetic process / miRNA-mediated post-transcriptional gene silencing / positive regulation of protein sumoylation / negative regulation of G2/M transition of mitotic cell cycle / miRNA-mediated gene silencing by inhibition of translation / stem cell division / protein localization to nucleolus / ribosomal protein import into nucleus / regulation of G1 to G0 transition / negative regulation of cell-cell adhesion / regulation of reactive oxygen species metabolic process / protein-DNA complex disassembly / regulation of glycolytic process / negative regulation of formation of translation preinitiation complex / stem cell population maintenance / GAIT complex / maturation of 5.8S rRNA / G1 to G0 transition / positive regulation of dendritic spine development / negative regulation of DNA replication / negative regulation of myoblast fusion / ribosomal large subunit binding / preribosome, large subunit precursor / PELO:HBS1L and ABCE1 dissociate a ribosome on a non-stop mRNA / positive regulation of telomere maintenance / ZNF598 and the Ribosome-associated Quality Trigger (RQT) complex dissociate a ribosome stalled on a no-go mRNA / Notch signaling pathway / Peptide chain elongation / protein targeting / Selenocysteine synthesis / Formation of a pool of free 40S subunits / mitotic metaphase chromosome alignment / Eukaryotic Translation Termination / nuclear-transcribed mRNA catabolic process / protein localization to nucleus / SRP-dependent cotranslational protein targeting to membrane / Response of EIF2AK4 (GCN2) to amino acid deficiency / negative regulation of ubiquitin-dependent protein catabolic process / ubiquitin ligase inhibitor activity / Viral mRNA Translation / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / positive regulation of signal transduction by p53 class mediator / ribosomal subunit export from nucleus / GTP hydrolysis and joining of the 60S ribosomal subunit / L13a-mediated translational silencing of Ceruloplasmin expression / Major pathway of rRNA processing in the nucleolus and cytosol / maturation of LSU-rRNA / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rough endoplasmic reticulum / Ribosome Quality Control (RQC) complex extracts and degrades nascent peptide / negative regulation of protein ubiquitination / translation initiation factor activity / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / negative regulation of cell migration / regulation of signal transduction by p53 class mediator / cytosolic ribosome assembly / condensed nuclear chromosome / ribosomal large subunit biogenesis / positive regulation of translation / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / assembly of large subunit precursor of preribosome / striated muscle contraction / cytosolic ribosome / mRNA 3'-UTR binding / chromosome segregation / cellular response to type II interferon / response to insulin / positive regulation of miRNA transcription / mRNA 5'-UTR binding / transcription coactivator binding / osteoblast differentiation / fibrillar center / Regulation of expression of SLITs and ROBOs / cytoplasmic ribonucleoprotein granule / rRNA processing / regulation of cell population proliferation / chromosome / large ribosomal subunit / Dengue Virus-Host Interactions / ribosome binding / ribosome biogenesis / 5S rRNA binding / cell body / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / spermatogenesis / cytosolic large ribosomal subunit / nuclear body / cytoplasmic translation / tRNA binding / postsynaptic density

Domain/homology:

Coiled-coil domain-containing protein 86 / Ribosome biogenesis protein Rpf2 / Learning associated protein 18-like / LLP homolog / Ribosomal biogenesis regulatory protein / Ribosome biogenesis regulatory protein (RRS1) / Nucleolar GTP-binding protein 2, N-terminal domain / Nucleolar GTP-binding protein 2 / NGP1NT (NUC091) domain / NLE / NLE (NUC135) domain / Guanine nucleotide-binding protein-like 3, N-terminal domain / GNL3L/Grn1 putative GTPase / : / Ribosomal biogenesis NSA2 family / GTP-binding protein, orthogonal bundle domain superfamily / Ribosome assembly factor Mrt4 / : / NOG, C-terminal / Nucleolar GTP-binding protein 1 / NOGCT (NUC087) domain / Nucleolar GTP-binding protein 1, Rossman-fold domain / NOG1, N-terminal helical domain / Nucleolar GTP-binding protein 1 (NOG1) / NOG1 N-terminal helical domain / : / Circularly permuted (CP)-type guanine nucleotide-binding (G) domain / Circularly permuted (CP)-type guanine nucleotide-binding (G) domain profile. / Brix domain / Brix domain / Brix domain profile. / Brix / OBG-type guanine nucleotide-binding (G) domain / OBG-type guanine nucleotide-binding (G) domain profile. / Translation initiation factor IF6 / eIF-6 family / translation initiation factor 6 / 50S ribosome-binding GTPase / GTP binding domain / Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / 50S ribosomal protein L10, insertion domain superfamily / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / Ribosomal protein L28e / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / metallochaperone-like domain / TRASH domain / : / Ribosomal protein L6e signature. / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal Protein L6, KOW domain / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein L35Ae, conserved site / Ribosomal protein L35Ae signature. / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / : / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L6e / 60S ribosomal protein L6E / Ribosomal protein L7, eukaryotic / Ribosomal protein L30, N-terminal / Ribosomal L30 N-terminal domain / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L4 C-terminal domain / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L14e domain / Ribosomal protein L14 / : / Ribosomal protein L18e / Ribosomal protein L35A / Ribosomal protein L35Ae / Ribosomal protein L35A superfamily / Ribosomal protein L32e, conserved site / Ribosomal protein L32e signature. / Ribosomal protein L6, conserved site-2 / Ribosomal protein L6 signature 2. / Ribosomal protein L14 / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal large subunit proteins 60S L5, and 50S L18 / Ribosomal protein L4/L1e, eukaryotic/archaeal, conserved site / Ribosomal protein L1e signature. / Ribosomal protein L21e / Ribosomal protein L21e, conserved site / Ribosomal protein L21 superfamily / Ribosomal protein L21e / Ribosomal protein L21e signature. / Ribosomal protein L24e-related / Ribosomal protein L24e/L24 superfamily / Ribosomal protein L24e / Ribosomal protein L3, domain 3, archaeal type superfamily / Ribosomal protein L3, archaeal/eukaryotic type

Component:

Ribosome biogenesis protein NSA2 homolog / Large ribosomal subunit protein eL33 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein eL28 / Large ribosomal subunit protein eL14 / Eukaryotic translation initiation factor 6 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein eL20 / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein eL18 / Nucleolar GTP-binding protein 2 / Ribosome biogenesis regulatory protein homolog / Protein LLP homolog / Guanine nucleotide-binding protein-like 3 / GTP-binding protein 4 / Coiled-coil domain-containing protein 86 / Ribosome production factor 2 homolog / Notchless protein homolog 1 / Probable ribosome biogenesis protein RLP24 / mRNA turnover protein 4 homolog

• Biological species: Homo sapiens (human)
• Method: single particle reconstruction / cryo EM / Resolution: 2.91 Å
• Authors: Vanden Broeck A / Klinge S

Funding support

European Union, United States, 2 items

Organization	Grant number	Country
European Molecular Biology Organization (EMBO)	ALTF 711-2019	European Union
The G. Harold and Leila Y. Mathers Foundation	MF-2104-01554	United States

• Citation: Journal: Science / Year: 2023; Title: Principles of human pre-60 biogenesis.; Authors: Arnaud Vanden Broeck / Sebastian Klinge / ; Abstract: During the early stages of human large ribosomal subunit (60) biogenesis, an ensemble of assembly factors establishes and fine-tunes the essential RNA functional centers of pre-60 particles by an unknown mechanism. Here, we report a series of cryo-electron microscopy structures of human nucleolar and nuclear pre-60 assembly intermediates at resolutions of 2.5 to 3.2 angstroms. These structures show how protein interaction hubs tether assembly factor complexes to nucleolar particles and how guanosine triphosphatases and adenosine triphosphatase couple irreversible nucleotide hydrolysis steps to the installation of functional centers. Nuclear stages highlight how a conserved RNA-processing complex, the rixosome, couples large-scale RNA conformational changes with pre-ribosomal RNA processing by the RNA degradation machinery. Our ensemble of human pre-60 particles provides a rich foundation with which to elucidate the molecular principles of ribosome formation.

History

Deposition	Dec 21, 2022	-
Header (metadata) release	Jul 12, 2023	-
Map release	Jul 12, 2023	-
Update	Jun 19, 2024	-
Current status	Jun 19, 2024	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_29263.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.072 Å

Density

Contour Level	By AUTHOR: 0.9
Minimum - Maximum	-1.6377656 - 6.5792446
Average (Standard dev.)	0.021820514 (±0.12314983)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 480 480 480 Spacing 480 480 480
Cell	A=B=C: 514.56 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_29263_msk_1.map

Half map: #2

• File: emd_29263_half_map_1.map

Half map: #1

• File: emd_29263_half_map_2.map

Sample components

Entire : Human nucleolar pre-60S ribosomal subunit (State H)

• Entire: Name: Human nucleolar pre-60S ribosomal subunit (State H)

Components

• Complex: Human nucleolar pre-60S ribosomal subunit (State H)
  • Protein or peptide: 60S ribosomal protein L12
  • RNA: 28S rRNA
  • RNA: 5S rRNA
  • Protein or peptide: 60S ribosomal protein L11
  • Protein or peptide: 60S ribosomal protein L13a
  • Protein or peptide: 60S ribosomal protein L14
  • Protein or peptide: 60S ribosomal protein L18
  • Protein or peptide: 60S ribosomal protein L18a
  • Protein or peptide: 60S ribosomal protein L21
  • Protein or peptide: 60S ribosomal protein L23
  • Protein or peptide: 60S ribosomal protein L28
  • Protein or peptide: 60S ribosomal protein L3
  • Protein or peptide: 60S ribosomal protein L32
  • Protein or peptide: 60S ribosomal protein L35a
  • Protein or peptide: Guanine nucleotide-binding protein-like 3
  • Protein or peptide: Nucleolar GTP-binding protein 2
  • Protein or peptide: Ribosome production factor 2 homolog
  • Protein or peptide: Ribosome biogenesis protein NSA2 homolog
  • Protein or peptide: Notchless protein homolog 1
  • Protein or peptide: Protein LLP homolog
  • Protein or peptide: Coiled-coil domain-containing protein 86
  • Protein or peptide: 60S ribosomal protein L4
  • Protein or peptide: 60S ribosomal protein L5
  • Protein or peptide: 60S ribosomal protein L6
  • Protein or peptide: 60S ribosomal protein L7
  • Protein or peptide: 60S ribosomal protein L9
  • Protein or peptide: Eukaryotic translation initiation factor 6
  • Protein or peptide: mRNA turnover protein 4 homolog
  • Protein or peptide: GTP-binding protein 4
  • Protein or peptide: Ribosome biogenesis regulatory protein homolog
  • Protein or peptide: Probable ribosome biogenesis protein RLP24
• Ligand: MAGNESIUM ION
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: POTASSIUM ION
• Ligand: GUANOSINE-5'-DIPHOSPHATE
• Ligand: ZINC ION

Supramolecule #1: Human nucleolar pre-60S ribosomal subunit (State H)

• Supramolecule: Name: Human nucleolar pre-60S ribosomal subunit (State H) / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#31
• Source (natural): Organism: Homo sapiens (human) / Strain: HEK293F

Macromolecule #1: 60S ribosomal protein L12

• Macromolecule: Name: 60S ribosomal protein L12 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.847619 KDa

Sequence

String:

MPPKFDPNEI KVVYLRCTGG EVGATSALAP KIGPLGLSPK KVGDDIAKAT GDWKGLRITV KLTIQNRQAQ IEVVPSASAL IIKALKEPP RDRKKQKNIK HSGNITFDEI VNIARQMRHR SLARELSGTI KEILGTAQSV GCNVDGRHPH DIIDDINSGA V ECPAS

UniProtKB: Large ribosomal subunit protein uL11

Macromolecule #4: 60S ribosomal protein L11

• Macromolecule: Name: 60S ribosomal protein L11 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 20.288465 KDa

Sequence

String:

MAQDQGEKEN PMRELRIRKL CLNICVGESG DRLTRAAKVL EQLTGQTPVF SKARYTVRSF GIRRNEKIAV HCTVRGAKAE EILEKGLKV REYELRKNNF SDTGNFGFGI QEHIDLGIKY DPSIGIYGLD FYVVLGRPGF SIADKKRRTG CIGAKHRISK E EAMRWFQQ KYDGIILPGK

UniProtKB: Large ribosomal subunit protein uL5

Macromolecule #5: 60S ribosomal protein L13a

• Macromolecule: Name: 60S ribosomal protein L13a / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.633412 KDa

Sequence

String:

MAEVQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLP HKTKRGQAAL DRLKVFDGIP PPYDKKKRMV VPAALKVVRL KPTRKFAYLG RLAHEVGWKY QAVTATLEEK R KEKAKIHY RKKKQLMRLR KQAEKNVEKK IDKYTEVLKT HGLLV

UniProtKB: Large ribosomal subunit protein uL13

Macromolecule #6: 60S ribosomal protein L14

• Macromolecule: Name: 60S ribosomal protein L14 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.485016 KDa

Sequence

String:

MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTQVRRQAM PFKCMQLTDF ILKFPHSAHQ KYVRQAWQKA DINTKWAAT RWAKKIEARE RKAKMTDFDR FKVMKAKKMR NRIIKNEVKK LQKAALLKAS PKKAPGTKGT AAAAAAAAAA K VPAKKITA ASKKAPAQKV PAQKATGQKA APAPKAQKGQ KAPAQKAPAP KASGKKA

UniProtKB: Large ribosomal subunit protein eL14

Macromolecule #7: 60S ribosomal protein L18

• Macromolecule: Name: 60S ribosomal protein L18 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 21.687676 KDa

Sequence

String:

MGVDIRHNKD RKVRRKEPKS QDIYLRLLVK LYRFLARRTN STFNQVVLKR LFMSRTNRPP LSLSRMIRKM KLPGRENKTA VVVGTITDD VRVQEVPKLK VCALRVTSRA RSRILRAGGK ILTFDQLALD SPKGCGTVLL SGPRKGREVY RHFGKAPGTP H SHTKPYVR SKGRKFERAR GRRASRGYKN

UniProtKB: Large ribosomal subunit protein eL18

Macromolecule #8: 60S ribosomal protein L18a

• Macromolecule: Name: 60S ribosomal protein L18a / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 20.808514 KDa

Sequence

String:

MKASGTLREY KVVGRCLPTP KCHTPPLYRM RIFAPNHVVA KSRFWYFVSQ LKKMKKSSGE IVYCGQVFEK SPLRVKNFGI WLRYDSRSG THNMYREYRD LTTAGAVTQC YRDMGARHRA RAHSIQIMKV EEIAASKCRR PAVKQFHDSK IKFPLPHRVL R RQHKPRFT TKRPNTFF

UniProtKB: Large ribosomal subunit protein eL20

Macromolecule #9: 60S ribosomal protein L21

• Macromolecule: Name: 60S ribosomal protein L21 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 18.609988 KDa

Sequence

String:

MTNTKGKRRG TRYMFSRPFR KHGVVPLATY MRIYKKGDIV DIKGMGTVQK GMPHKCYHGK TGRVYNVTQH AVGIVVNKQV KGKILAKRI NVRIEHIKHS KSRDSFLKRV KENDQKKKEA KEKGTWVQLK RQPAPPREAH FVRTNGKEPE LLEPIPYEFM A

UniProtKB: Large ribosomal subunit protein eL21

Macromolecule #10: 60S ribosomal protein L23

• Macromolecule: Name: 60S ribosomal protein L23 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.892505 KDa

Sequence

String:

MSKRGRGGSS GAKFRISLGL PVGAVINCAD NTGAKNLYII SVKGIKGRLN RLPAAGVGDM VMATVKKGKP ELRKKVHPAV VIRQRKSYR RKDGVFLYFE DNAGVIVNNK GEMKGSAITG PVAKECADLW PRIASNAGSI A

UniProtKB: Large ribosomal subunit protein uL14

Macromolecule #11: 60S ribosomal protein L28

• Macromolecule: Name: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.784622 KDa

Sequence

String:

MSAHLQWMVV RNCSSFLIKR NKQTYSTEPN NLKARNSFRY NGLIHRKTVG VEPAADGKGV VVVIKRRSGQ RKPATSYVRT TINKNARAT LSSIRHMIRK NKYRPDLRMA AIRRASAILR SQKPVMVKRK RTRPTKSS

UniProtKB: Large ribosomal subunit protein eL28

Macromolecule #12: 60S ribosomal protein L3

• Macromolecule: Name: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 46.224133 KDa

Sequence

String:

MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD EDGKKQLEKD FSSMKKYCQV I RVIAHTQM RLLPLRQKKA HLMEIQVNGG TVAEKLDWAR ERLEQQVPVN QVFGQDEMID VIGVTKGKGY KGVTSRWHTK KL PRKT(HIC)RG LRKVACIGAW HPARVAFSVA RAGQKGYHHR TEINKKIYKI GQGYLIKDGK LIKNNASTDY DLSDKSINP LGGFVHYGEV TNDFVMLKGC VVGTKKRVLT LRKSLLVQTK RRALEKIDLK FIDTTSKFGH GRFQTMEEKK AFMGPLKKDR IAKEEGA

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #13: 60S ribosomal protein L32

• Macromolecule: Name: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.898932 KDa

Sequence

String:

MAALRPLVKP KIVKKRTKKF IRHQSDRYVK IKRNWRKPRG IDNRVRRRFK GQILMPNIGY GSNKKTKHML PSGFRKFLVH NVKELEVLL MCNKSYCAEI AHNVSSKNRK AIVERAAQLA IRVTNPNARL RSEENE

UniProtKB: Large ribosomal subunit protein eL32

Macromolecule #14: 60S ribosomal protein L35a

• Macromolecule: Name: 60S ribosomal protein L35a / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 12.564743 KDa

Sequence

String:

MSGRLWSKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV YKAKNNTVTP GGKPNKTRVI WGKVTRAHGN SGMVRAKFR SNLPAKAIGH RIRVMLYPSR I

UniProtKB: Large ribosomal subunit protein eL33

Macromolecule #15: Guanine nucleotide-binding protein-like 3

• Macromolecule: Name: Guanine nucleotide-binding protein-like 3 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 62.098242 KDa

Sequence

String:

MKRPKLKKAS KRMTCHKRYK IQKKVREHHR KLRKEAKKRG HKKPRKDPGV PNSAPFKEAL LREAELRKQR LEELKQQQKL DRQKELEKK RKLETNPDIK PSNVEPMEKE FGLCKTENKA KSGKQNSKKL YCQELKKVIE ASDVVLEVLD ARDPLGCRCP Q VEEAIVQS GQKKLVLILN KSDLVPKENL ESWLNYLKKE LPTVVFRAST KPKDKGKITK RVKAKKNAAP FRSEVCFGKE GL WKLLGGF QETCSKAIRV GVIGFPNVGK SSIINSLKQE QMCNVGVSMG LTRSMQVVPL DKQITIIDSP SFIVSPLNSS SAL ALRSPA SIEVVKPMEA ASAILSQADA RQVVLKYTVP GYRNSLEFFT VLAQRRGMHQ KGGIPNVEGA AKLLWSEWTG ASLA YYCHP PTSWTPPPYF NESIVVDMKS GFNLEELEKN NAQSIRAIKG PHLANSILFQ SSGLTNGIIE EKDIHEELPK RKERK QEER EDDKDSDQET VDEEVDENSS GMFAAEETGE ALSEETTAGE QSTRSFILDK IIEEDDAYDF STDYV

UniProtKB: Guanine nucleotide-binding protein-like 3

Macromolecule #16: Nucleolar GTP-binding protein 2

• Macromolecule: Name: Nucleolar GTP-binding protein 2 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 83.796094 KDa

Sequence

String:

MVKPKYKGRS TINPSKASTN PDRVQGAGGQ NMRDRATIRR LNMYRQKERR NSRGKIIKPL QYQSTVASGT VARVEPNIKW FGNTRVIKQ SSLQKFQEEM DTVMKDPYKV VMKQSKLPMS LLHDRIRPHN LKVHILDTES FETTFGPKSQ RKRPNLFASD M QSLIENAE MSTESYDQGK DRDLVTEDTG VRNEAQEEIY KKGQSKRIWG ELYKVIDSSD VVVQVLDARD PMGTRSPHIE TY LKKEKPW KHLIFVLNKC DLVPTWATKR WVAVLSQDYP TLAFHASLTN PFGKGAFIQL LRQFGKLHTD KKQISVGFIG YPN VGKSSV INTLRSKKVC NVAPIAGETK VWQYITLMRR IFLIDCPGVV YPSEDSETDI VLKGVVQVEK IKSPEDHIGA VLER AKPEY ISKTYKIDSW ENAEDFLEKL AFRTGKLLKG GEPDLQTVGK MVLNDWQRGR IPFFVKPPNA EPLVAPQLLP SSSLE VVPE AAQNNPGEEV TETAGEGSES IIKEETEENS HCDANTEMQQ ILTRVRQNFG KINVVPQFSG DDLVPVEVSD LEEELE SFS DEEEEEQEQQ RDDAEESSSE PEEENVGNDT KAVIKALDEK IAKYQKFLDK AKAKKFSAVR ISKGLSEKIF AKPEEQR KT LEEDVDDRAP SKKGKKRKAQ REEEQEHSNK APRALTSKER RRAVRQQRPK KVGVRYYETH NVKNRNRNKK KTNDSEGQ K HKRKKFRQKQ

UniProtKB: Nucleolar GTP-binding protein 2

Macromolecule #17: Ribosome production factor 2 homolog

• Macromolecule: Name: Ribosome production factor 2 homolog / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 35.658078 KDa

Sequence

String:

MDTLDRVVKP KTKRAKRFLE KREPKLNENI KNAMLIKGGN ANATVTKVLK DVYALKKPYG VLYKKKNITR PFEDQTSLEF FSKKSDCSL FMFGSHNKKR PNNLVIGRMY DYHVLDMIEL GIENFVSLKD IKNSKCPEGT KPMLIFAGDD FDVTEDYRRL K SLLIDFFR GPTVSNIRLA GLEYVLHFTA LNGKIYFRSY KLLLKKSGCR TPRIELEEMG PSLDLVLRRT HLASDDLYKL SM KMPKALK PKKKKNISHD TFGTTYGRIH MQKQDLSKLQ TRKMKGLKKR PAERITEDHE KKSKRIKKN

UniProtKB: Ribosome production factor 2 homolog

Macromolecule #18: Ribosome biogenesis protein NSA2 homolog

• Macromolecule: Name: Ribosome biogenesis protein NSA2 homolog / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 30.136703 KDa

Sequence

String:

MPQNEYIELH RKRYGYRLDY HEKKRKKESR EAHERSKKAK KMIGLKAKLY HKQRHAEKIQ MKKTIKMHEK RNTKQKNDEK TPQGAVPAY LLDREGQSRA KVLSNMIKQK RKEKAGKWEV PLPKVRAQGE TEVLKVIRTG KRKKKAWKRM VTKVCFVGDG F TRKPPKYE RFIRPMGLRF KKAHVTHPEL KATFCLPILG VKKNPSSPLY TTLGVITKGT VIEVNVSELG LVTQGGKVIW GK YAQVTNN PENDGCINAV LLV

UniProtKB: Ribosome biogenesis protein NSA2 homolog

Macromolecule #19: Notchless protein homolog 1

• Macromolecule: Name: Notchless protein homolog 1 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 53.387141 KDa

Sequence

String:

MAAAVPDEAV ARDVQRLLVQ FQDEGGQLLG SPFDVPVDIT PDRLQLVCNA LLAQEDPLPL AFFVHDAEIV SSLGKTLESQ AVETEKVLD IIYQPQAIFR VRAVTRCTSS LEGHSEAVIS VAFSPTGKYL ASGSGDTTVR FWDLSTETPH FTCKGHRHWV L SISWSPDG RKLASGCKNG QILLWDPSTG KQVGRTLAGH SKWITGLSWE PLHANPECRY VASSSKDGSV RIWDTTAGRC ER ILTGHTQ SVTCLRWGGD GLLYSASQDR TIKVWRAHDG VLCRTLQGHG HWVNTMALST DYALRTGAFE PAEASVNPQD LQG SLQELK ERALSRYNLV RGQGPERLVS GSDDFTLFLW SPAEDKKPLT RMTGHQALIN QVLFSPDSRI VASASFDKSI KLWD GRTGK YLASLRGHVA AVYQIAWSAD SRLLVSGSSD STLKVWDVKA QKLAMDLPGH ADEVYAVDWS PDGQRVASGG KDKCL RIWR R

UniProtKB: Notchless protein homolog 1

Macromolecule #20: Protein LLP homolog

• Macromolecule: Name: Protein LLP homolog / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.268361 KDa

Sequence

String:

MAKSLRSKWK RKMRAEKRKK NAPKEASRLK SILKLDGDVL MKDVQEIATV VVPKPKHCQE KMQCEVKDEK DDMKMETDIK RNKKTLLDQ HGQYPIWMNQ RQRKRLKAKR EKRKGKSKAK AVKVAKGLAW

UniProtKB: Protein LLP homolog

Macromolecule #21: Coiled-coil domain-containing protein 86

• Macromolecule: Name: Coiled-coil domain-containing protein 86 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 40.312742 KDa

Sequence

String:

MDTPLRRSRR LGGLRPESPE SLTSVSRTRR ALVEFESNPE ETREPGSPPS VQRAGLGSPE RPPKTSPGSP RLQQGAGLES PQGQPEPGA ASPQRQQDLH LESPQRQPEY SPESPRCQPK PSEEAPKCSQ DQGVLASELA QNKEELTPGA PQHQLPPVPG S PEPYPGQQ APGPEPSQPL LELTPRAPGS PRGQHEPSKP PPAGETVTGG FGAKKRKGSS SQAPASKKLN KEELPVIPKG KP KSGRVWK DRSKKRFSQM LQDKPLRTSW QRKMKERQER KLAKDFARHL EEEKERRRQE KKQRRAENLK RRLENERKAE VVQ VIRNPA KLKRAKKKQL RSIEKRDTLA LLQKQPPQQP AAKI

UniProtKB: Coiled-coil domain-containing protein 86

Macromolecule #22: 60S ribosomal protein L4

• Macromolecule: Name: 60S ribosomal protein L4 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 47.804621 KDa

Sequence

String:

MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVEDKVE G YKKTKEAV LLLKKLKAWN DIKKVYASQR MRAGKGKMRN RRRIQRRGPC IIYNEDNGII KAFRNIPGIT LLNVSKLNIL KL APGGHVG RFCIWTESAF RKLDELYGTW RKAASLKSNY NLPMHKMINT DLSRILKSPE IQRALRAPRK KIHRRVLKKN PLK NLRIML KLNPYAKTMR RNTILRQARN HKLRVDKAAA AAAALQAKSD EKAAVAGKKP VVGKKGKKAA VGVKKQKKPL VGKK AAATK KPAPEKKPAE KKPTTEEKKP AA

UniProtKB: Large ribosomal subunit protein uL4

Macromolecule #23: 60S ribosomal protein L5

• Macromolecule: Name: 60S ribosomal protein L5 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 34.426789 KDa

Sequence

String:

MGFVKVVKNK AYFKRYQVKF RRRREGKTDY YARKRLVIQD KNKYNTPKYR MIVRVTNRDI ICQIAYARIE GDMIVCAAYA HELPKYGVK VGLTNYAAAY CTGLLLARRL LNRFGMDKIY EGQVEVTGDE YNVESIDGQP GAFTCYLDAG LARTTTGNKV F GALKGAVD GGLSIPHSTK RFPGYDSESK EFNAEVHRKH IMGQNVADYM RYLMEEDEDA YKKQFSQYIK NSVTPDMMEE MY KKAHAAI RENPVYEKKP KKEVKKKRWN RPKMSLAQKK DRVAQKKASF LRAQERAAES

UniProtKB: Large ribosomal subunit protein uL18

Macromolecule #24: 60S ribosomal protein L6

• Macromolecule: Name: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 32.810176 KDa

Sequence

String:

MAGEKVEKPD TKEKKPEAKK VDAGGKVKKG NLKAKKPKKG KPHCSRNPVL VRGIGRYSRS AMYSRKAMYK RKYSAAKSKV EKKKKEKVL ATVTKPVGGD KNGGTRVVKL RKMPRYYPTE DVPRKLLSHG KKPFSQHVRK LRASITPGTI LIILTGRHRG K RVVFLKQL ASGLLLVTGP LVLNRVPLRR THQKFVIATS TKIDISNVKI PKHLTDAYFK KKKLRKPRHQ EGEIFDTEKE KY EITEQRK IDQKAVDSQI LPKIKAIPQL QGYLRSVFAL TNGIYPHKLV F

UniProtKB: Large ribosomal subunit protein eL6

Macromolecule #25: 60S ribosomal protein L7

• Macromolecule: Name: 60S ribosomal protein L7 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.290973 KDa

Sequence

String:

MEGVEEKKKE VPAVPETLKK KRRNFAELKI KRLRKKFAQK MLRKARRKLI YEKAKHYHKE YRQMYRTEIR MARMARKAGN FYVPAEPKL AFVIRIRGIN GVSPKVRKVL QLLRLRQIFN GTFVKLNKAS INMLRIVEPY IAWGYPNLKS VNELIYKRGY G KINKKRIA LTDNALIARS LGKYGIICME DLIHEIYTVG KRFKEANNFL WPFKLSSPRG GMKKKTTHFV EGGDAGNRED QI NRLIRRM N

UniProtKB: Large ribosomal subunit protein uL30

Macromolecule #26: 60S ribosomal protein L9

• Macromolecule: Name: 60S ribosomal protein L9 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 21.899471 KDa

Sequence

String:

MKTILSNQTV DIPENVDITL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELILEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QADE

UniProtKB: Large ribosomal subunit protein uL6

Macromolecule #27: Eukaryotic translation initiation factor 6

• Macromolecule: Name: Eukaryotic translation initiation factor 6 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 26.62001 KDa

Sequence

String:

MAVRASFENN CEIGCFAKLT NTYCLVAIGG SENFYSVFEG ELSDTIPVVH ASIAGCRIIG RMCVGNRHGL LVPNNTTDQE LQHIRNSLP DTVQIRRVEE RLSALGNVTT CNDYVALVHP DLDRETEEIL ADVLKVEVFR QTVADQVLVG SYCVFSNQGG L VHPKTSIE DQDELSSLLQ VPLVAGTVNR GSEVIAAGMV VNDWCAFCGL DTTSTELSVV ESVFKLNEAQ PSTIATSMRD SL IDSLT

UniProtKB: Eukaryotic translation initiation factor 6

Macromolecule #28: mRNA turnover protein 4 homolog

• Macromolecule: Name: mRNA turnover protein 4 homolog / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 27.602535 KDa

Sequence

String:

MPKSKRDKKV SLTKTAKKGL ELKQNLIEEL RKCVDTYKYL FIFSVANMRN SKLKDIRNAW KHSRMFFGKN KVMMVALGRS PSDEYKDNL HQVSKRLRGE VGLLFTNRTK EEVNEWFTKY TEMDYARAGN KAAFTVSLDP GPLEQFPHSM EPQLRQLGLP T ALKRGVVT LLSDYEVCKE GDVLTPEQAR VLKLFGYEMA EFKVTIKYMW DSQSGRFQQM GDDLPESASE STEESDSEDD D

UniProtKB: mRNA turnover protein 4 homolog

Macromolecule #29: GTP-binding protein 4

• Macromolecule: Name: GTP-binding protein 4 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 74.10782 KDa

Sequence

String:

MAHYNFKKIT VVPSAKDFID LTLSKTQRKT PTVIHKHYQI HRIRHFYMRK VKFTQQNYHD RLSQILTDFP KLDDIHPFYA DLMNILYDK DHYKLALGQI NIAKNLVDNV AKDYVRLMKY GDSLYRCKQL KRAALGRMCT VIKRQKQSLE YLEQVRQHLS R LPTIDPNT RTLLLCGYPN VGKSSFINKV TRADVDVQPY AFTTKSLFVG HMDYKYLRWQ VVDTPGILDH PLEDRNTIEM QA ITALAHL RAAVLYVMDL SEQCGHGLRE QLELFQNIRP LFINKPLIVV ANKCDVKRIA ELSEDDQKIF TDLQSEGFPV IET STLTEE GVIKVKTEAC DRLLAHRVET KMKGNKVNEV LNRLHLAIPT RRDDKERPPF IPEGVVARRK RMETEESRKK RERD LELEM GDDYILDLQK YWDLMNLSEK HDKIPEIWEG HNIADYIDPA IMKKLEELEK EEELRTAAGE YDSVSESEDE EMLEI RQLA KQIREKKKLK ILESKEKNTQ GPRMPRTAKK VQRTVLEKEM RSLGVDMDDK DDAHYAVQAR RSRSITRKRK REDSAP PSS VARSGSCSRT PRDVSGLRDV KMVKKAKTMM KNAQKKMNRL GKKGEADRHV FDMKPKHLLS GKRKAGKKDR R

UniProtKB: GTP-binding protein 4

Macromolecule #30: Ribosome biogenesis regulatory protein homolog

• Macromolecule: Name: Ribosome biogenesis regulatory protein homolog / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 41.278523 KDa

Sequence

String:

MEGQSVEELL AKAEQDEAEK LQRITVHKEL ELQFDLGNLL ASDRNPPTGL RCAGPTPEAE LQALARDNTQ LLINQLWQLP TERVEEAIV ARLPEPTTRL PREKPLPRPR PLTRWQQFAR LKGIRPKKKT NLVWDEVSGQ WRRRWGYQRA RDDTKEWLIE V PGNADPLE DQFAKRIQAK KERVAKNELN RLRNLARAHK MQLPSAAGLH PTGHQSKEEL GRAMQVAKVS TASVGRFQER LP KEKVPRG SGKKRKFQPL FGDFAAEKKN QLELLRVMNS KKPQLDVTRA TNKQMREEDQ EEAAKRRKMS QKGKRKGGRQ GPG GKRKGG PPSQGGKRKG GLGGKMNSGP PGLGGKRKGG QRPGGKRRK

UniProtKB: Ribosome biogenesis regulatory protein homolog

Macromolecule #31: Probable ribosome biogenesis protein RLP24

• Macromolecule: Name: Probable ribosome biogenesis protein RLP24 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 19.666258 KDa

Sequence

String:

MRIEKCYFCS GPIYPGHGMM FVRNDCKVFR FCKSKCHKNF KKKRNPRKVR WTKAFRKAAG KELTVDNSFE FEKRRNEPIK YQRELWNKT IDAMKRVEEI KQKRQAKFIM NRLKKNKELQ KVQDIKEVKQ NIHLIRAPLA GKGKQLEEKM VQQLQEDVDM E DAP

UniProtKB: Probable ribosome biogenesis protein RLP24

Macromolecule #2: 28S rRNA

• Macromolecule: Name: 28S rRNA / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 1.640222125 MDa

Sequence

String:

CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAG AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGCGGG GCGCGGGACA UGUGGCGUAC G GAAGACCC GCUCCCCGGC GCCGCUCGUG GGGGGCCCAA GUCCUUCUGA UCGAGGCCCA GCCCGUGGAC GGUGUGAGGC CG GUAGCGG CCCCCGGCGC GCCGGGCCCG GGUCUUCCCG GAGUCGGGUU GCUUGGGAAU GCAGCCCAAA GCGGGUGGUA AAC UCCAUC UAAGGCUAAA UACCGGCACG AGACCGAUAG UCAACAAGUA CCGUAAGGGA AAGUUGAAAA GAACUUUGAA GAGA GAGUU CAAGAGGGCG UGAAACCGUU AAGAGGUAAA CGGGUGGGGU CCGCGCAGUC CGCCCGGAGG AUUCAACCCG GCGGC GGGU CCGGCCGUGU CGGCGGCCCG GCGGAUCUUU CCCGCCCCCC GUUCCUCCCG ACCCCUCCAC CCGCCCUCCC UUCCCC CGC CGCCCCUCCU CCUCCUCCCC GGAGGGGGCG GGCUCCGGCG GGUGCGGGGG UGGGCGGGCG GGGCCGGGGG UGGGGUC GG CGGGGGACCG UCCCCCGACC GGCGACCGGC CGCCGCCGGG CGCAUUUCCA CCGCGGCGGU GCGCCGCGAC CGGCUCCG G GACGGCUGGG AAGGCCCGGC GGGGAAGGUG GCUCGGGGGG CCCCGUCCGU CCGUCCGUCC GUCCUCCUCC UCCCCCGUC UCCGCCCCCC GGCCCCGCGU CCUCCCUCGG GAGGGCGCGC GGGUCGGGGC GGCGGCGGCG GCGGCGGUGG CGGCGGCGGC GGCGGCGGC GGGACCGAAA CCCCCCCCGA GUGUUACAGC CCCCCCGGCA GCAGCACUCG CCGAAUCCCG GGGCCGAGGG A GCGAGACC CGUCGCCGCG CUCUCCCCCC UCCCGGCGCC CACCCCCGCG GGGAAUCCCC CGCGAGGGGG GUCUCCCCCG CG GGGGCGC GCCGGCGUCU CCUCGUGGGG GGGCCGGGCC ACCCCUCCCA CGGCGCGACC GCUCUCCCAC CCCUCCUCCC CGC GCCCCC GCCCCGGCGA CGGGGGGGGU GCCGCGCGCG GGUCGGGGGG CGGGGCGGAC UGUCCCCAGU GCGCCCCGGG CGGG UCGCG CCGUCGGGCC CGGGGGAGGU UCUCUCGGGG CCACGCGCGC GUCCCCCGAA GAGGGGGACG GCGGAGCGAG CGCAC GGGG UCGGCGGCGA CGUCGGCUAC CCACCCGACC CGUCUUGAAA CACGGACCAA GGAGUCUAAC ACGUGCGCGA GUCGGG GGC UCGCACGAAA GCCGCCGUGG CGCAAUGAAG GUGAAGGCCG GCGCGCUCGC CGGCCGAGGU GGGAUCCCGA GGCCUCU CC AGUCCGCCGA GGGCGCACCA CCGGCCCGUC UCGCCCGCCG CGCCGGGGAG GUGGAGCACG AGCGCACGUG UUAGGACC C GAAAGAUGGU GAACUAUGCC UGGGCAGGGC GAAGCCAGAG GAAACUCUGG UGGAGGUCCG UAGCGGUCCU GACGUGCAA AUCGGUCGUC CGACCUGGGU AUAGGGGCGA AAGACUAAUC GAACCAUCUA GUAGCUGGUU CCCUCCGAAG UUUCCCUCAG GAUAGCUGG CGCUCUCGCA GACCCGACGC ACCCCCGCCA CGCAGUUUUA UCCGGUAAAG CGAAUGAUUA GAGGUCUUGG G GCCGAAAC GAUCUCAACC UAUUCUCAAA CUUUAAAUGG GUAAGAAGCC CGGCUCGCUG GCGUGGAGCC GGGCGUGGAA UG CGAGUGC CUAGUGGGCC ACUUUUGGUA AGCAGAACUG GCGCUGCGGG AUGAACCGAA CGCCGGGUUA AGGCGCCCGA UGC CGACGC UCAUCAGACC CCAGAAAAGG UGUUGGUUGA UAUAGACAGC AGGACGGUGG CCAUGGAAGU CGGAAUCCGC UAAG GAGUG UGUAACAACU CACCUGCCGA AUCAACUAGC CCUGAAAAUG GAUGGCGCUG GAGCGUCGGG CCCAUACCCG GCCGU CGCC GGCAGUCGAG AGUGGACGGG AGCGGCGGGG GCGGCGCGCG CGCGCGCGCG UGUGGUGUGC GUCGGAGGGC GGCGGC GGC GGCGGCGGCG GGGGUGUGGG GUCCUUCCCC CGCCCCCCCC CCCACGCCUC CUCCCCUCCU CCCGCCCACG CCCCGCU CC CCGCCCCCGG AGCCCCGCGG ACGCUACGCC GCGACGAGUA GGAGGGCCGC UGCGGUGAGC CUUGAAGCCU AGGGCGCG G GCCCGGGUGG AGCCGCCGCA GGUGCAGAUC UUGGUGGUAG UAGCAAAUAU UCAAACGAGA ACUUUGAAGG CCGAAGUGG AGAAGGGUUC CAUGUGAACA GCAGUUGAAC AUGGGUCAGU CGGUCCUGAG AGAUGGGCGA GCGCCGUUCC GAAGGGACGG GCGAUGGCC UCCGUUGCCC UCGGCCGAUC GAAAGGGAGU CGGGUUCAGA UCCCCGAAUC CGGAGUGGCG GAGAUGGGCG C CGCGAGGC GUCCAGUGCG GUAACGCGAC CGAUCCCGGA GAAGCCGGCG GGAGCCCCGG GGAGAGUUCU CUUUUCUUUG UG AAGGGCA GGGCGCCCUG GAAUGGGUUC GCCCCGAGAG AGGGGCCCGU GCCUUGGAAA GCGUCGCGGU UCCGGCGGCG UCC GGUGAG CUCUCGCUGG CCCUUGAAAA UCCGGGGGAG AGGGUGUAAA UCUCGCGCCG GGCCGUACCC AUAUCCGCAG CAGG UCUCC AAGGUGAACA GCCUCUGGCA UGUUGGAACA AUGUAGGUAA GGGAAGUCGG CAAGCCGGAU CCGUAACUUC GGGAU AAGG AUUGGCUCUA AGGGCUGGGU CGGUCGGGCU GGGGCGCGAA GCGGGGCUGG GCGCGCGCCG CGGCUGGACG AGGCGC CGC CGCCCCCCCC ACGCCCGGGG CACCCCCCUC GCGGCCCUCC CCCGCCCCAC CCCGCGCGCG CCGCUCGCUC CCUCCCC GC CCCGCGCCCU CUCUCUCUCU CUCUCCCCCG CUCCCCGUCC UCCCCCCUCC CCGGGGGAGC GCCGCGUGGG GGCGGCGG C GGGGGGAGAA GGGUCGGGGC GGCAGGGGCC GGCGGCGGCC CGCCGCGGGG CCCCGGCGGC GGGGGCACGG UCCCCCGCG AGGGGGGCCC GGGCACCCGG GGGGCCGGCG GCGGCGGCGA CUCUGGACGC GAGCCGGGCC CUUCCCGUGG AUCGCCCCAG CUGCGGCGG GCGUCGCGGC CGCCCCCGGG GAGCCCGGCG GGCGCCGGCG CGCCCCCCCC CCCACCCCAC GUCUCGUCGC G CGCGCGUC CGCUGGGGGC GGGGAGCGGU CGGGCGGCGG CGGUCGGCGG GCGGCGGGGC GGGGCGGUUC GUCCCCCCGC CC UACCCCC CCGGCCCCGU CCGCCCCCCG UUCCCCCCUC CUCCUCGGCG CGCGGCGGCG GCGGCGGCAG GCGGCGGAGG GGC CGCGGG CCGGUCCCCC CCGCCGGGUC CGCCCCCGGG GCCGCGGUUC CGCGCGGCGC CUCGCCUCGG CCGGCGCCUA GCAG CCGAC UUAGAACUGG UGCGGACCAG GGGAAUCCGA CUGUUUAAUU AAAACAAAGC AUCGCGAAGG CCCGCGGCGG GUGUU GACG CGAUGUGAUU UCUGCCCAGU GCUCUGAAUG UCAAAGUGAA GAAAUUCAAU GAAGCGCGGG UAAACGGCGG GAGUAA CUA UGACUCUCUU AAGGUAGCCA AAUGCCUCGU CAUCUAAUUA GUGACGCGCA UGAAUGGAUG AACGAGAUUC CCACUGU CC CUACCUACUA UCCAGCGAAA CCACAGCCAA GGGAACGGGC UUGGCGGAAU CAGCGGGGAA AGAAGACCCU GUUGAGCU U GACUCUAGUC UGGCACGGUG AAGAGACAUG AGAGGUGUAG AAUAAGUGGG AGGCCCCCGG CGCCCCCCCG GUGUCCCCG CGAGGGGCCC GGGGCGGGGU CCGCCGGCCC UGCGGGCCGC CGGUGAAAUA CCACUACUCU GAUCGUUUUU UCACUGACCC GGUGAGGCG GGGGGGCGAG CCCCGAGGGG CUCUCGCUUC UGGCGCCAAG CGCCCGGCCG CGCGCCGGCC GGGCGCGACC C GCUCCGGG GACAGUGCCA GGUGGGGAGU UUGACUGGGG CGGUACACCU GUCAAACGGU AACGCAGGUG UCCUAAGGCG AG CUCAGGG AGGACAGAAA CCUCCCGUGG AGCAGAAGGG CAAAAGCUCG CUUGAUCUUG AUUUUCAGUA CGAAUACAGA CCG UGAAAG CGGGGCCUCA CGAUCCUUCU GACCUUUUGG GUUUUAAGCA GGAGGUGUCA GAAAAGUUAC CACAGGGAUA ACUG GCUUG UGGCGGCCAA GCGUUCAUAG CGACGUCGCU UUUUGAUCCU UCGAUGUCGG CUCUUCCUAU CAUUGUGAAG CAGAA UUCA CCAAGCGUUG GAUUGUUCAC CCACUAAUAG GGAACGUGAG CUGGGUUUAG ACCGUCGUGA GACAGGUUAG UUUUAC CCU ACUGAUGAUG UGUUGUUGCC AUGGUAAUCC UGCUCAGUAC GAGAGGAACC GCAGGUUCAG ACAUUUGGUG UAUGUGC UU GGCUGAGGAG CCAAUGGGGC GAAGCUACCA UCUGUGGGAU UAUGACUGAA CGCCUCUAAG UCAGAAUCCC GCCCAGGC G GAACGAUACG GCAGCGCCGC GGAGCCUCGG UUGGCCUCGG AUAGCCGGUC CCCCGCCUGU CCCCGCCGGC GGGCCGCCC CCCCCCUCCA CGCGCCCCGC GCGCGCGGGA GGGCGCGUGC CCCGCCGCGC GCCGGGACCG GGGUCCGGUG CGGAGUGCCC UUCGUCCUG GGAAACGGGG CGCGGCCGGA GAGGCGGCCG CCCCCUCGCC CGUCACGCAC CGCACGUUCG UGGGGAACCU G GCGCUAAA CCAUUCGUAG ACGACCUGCU UCUGGGUCGG GGUUUCGUAC GUAGCAGAGC AGCUCCCUCG CUGCGAUCUA UU GAAAGUC AGCCCUCGAC ACAAGGGUUU GUC

GENBANK: GENBANK: AL592188.60

Macromolecule #3: 5S rRNA

• Macromolecule: Name: 5S rRNA / type: rna / ID: 3 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 38.998078 KDa

Sequence

String:

GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAG ACCGCCUGGG AAUACCGGGU GCUGUAGGCU UU

GENBANK: GENBANK: X12811.1

Macromolecule #32: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 32 / Number of copies: 22 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #33: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 33 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #34: POTASSIUM ION

• Macromolecule: Name: POTASSIUM ION / type: ligand / ID: 34 / Number of copies: 2 / Formula: K
• Molecular weight: Theoretical: 39.098 Da

Macromolecule #35: GUANOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 35 / Number of copies: 1 / Formula: GDP
• Molecular weight: Theoretical: 443.201 Da

Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Macromolecule #36: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 36 / Number of copies: 1 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.6
• Grid: Model: Quantifoil R3.5/1 / Material: GOLD / Mesh: 400 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec.
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 283 K / Instrument: FEI VITROBOT MARK IV; Details: Four applications with manual blotting before last blotting with the vitrobot..

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Specialist optics: Energy filter - Slit width: 20 eV
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 4 / Number real images: 172699 / Average exposure time: 2.0 sec. / Average electron dose: 60.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 64000
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 15679142
• Startup model: Type of model: INSILICO MODEL
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.91 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 3.3.2) / Number images used: 67272
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 4.0)
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.3.2)
• Final 3D classification: Software: (Name: RELION (ver. 4.0), cryoSPARC (ver. 3.3.2))

Atomic model buiding 1

• Refinement: Space: REAL

Output model

PDB-8fl0:
Human nucleolar pre-60S ribosomal subunit (State H)