EMDB-29261: Human nucleolar pre-60S ribosomal subunit (State F) - Composite map

Basic information

Entry

Database: EMDB / ID: EMD-29261

• Title: Human nucleolar pre-60S ribosomal subunit (State F) - Composite map

Sample

• Complex: Human nucleolar pre-60S ribosomal subunit (State F)
  • Protein or peptide: x 56 types
  • RNA: x 3 types
• Ligand: x 5 types

• Keywords: Pre-60S ribosomal subunit / Assembly intermediate / Ribosome / Nucleoprotein complex

Function / homology

Function:

RNA 2'-O-methyltransferase activity / Noc1p-Noc2p complex / Noc2p-Noc3p complex / RNA metabolic process / positive regulation of protein localization to chromosome, telomeric region / basal RNA polymerase II transcription machinery binding / rRNA (uridine-2'-O-)-methyltransferase activity / dendrite extension / TFAP2A acts as a transcriptional repressor during retinoic acid induced cell differentiation / rRNA (guanine) methyltransferase activity / rRNA (cytosine-C5-)-methyltransferase activity / preribosome binding / lamin filament / regulation of fatty acid biosynthetic process / RNA methylation / regulation of cellular senescence / regulation of megakaryocyte differentiation / miRNA-mediated post-transcriptional gene silencing / negative regulation of G2/M transition of mitotic cell cycle / PeBoW complex / positive regulation of protein sumoylation / miRNA-mediated gene silencing by inhibition of translation / blastocyst formation / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA base methylation / regulation of translation involved in cellular response to UV / negative regulation of formation of translation preinitiation complex / regulation of G1 to G0 transition / protein localization to nucleolus / ribosomal protein import into nucleus / protein-DNA complex disassembly / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / rRNA methylation / GAIT complex / positive regulation of DNA damage response, signal transduction by p53 class mediator / regulation of glycolytic process / TORC2 complex binding / G1 to G0 transition / regulation of reactive oxygen species metabolic process / negative regulation of cell-cell adhesion / negative regulation of B cell apoptotic process / maturation of 5.8S rRNA / stem cell division / rRNA modification in the nucleus and cytosol / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / preribosome, small subunit precursor / mitotic metaphase chromosome alignment / cytoplasmic side of rough endoplasmic reticulum membrane / stem cell population maintenance / negative regulation of ubiquitin protein ligase activity / rRNA metabolic process / homeostatic process / negative regulation of DNA replication / positive regulation of dendritic spine development / negative regulation of intrinsic apoptotic signaling pathway / fat cell differentiation / macrophage chemotaxis / ribosomal large subunit binding / lung morphogenesis / positive regulation of telomere maintenance / positive regulation of natural killer cell proliferation / preribosome, large subunit precursor / rRNA transcription / nuclear-transcribed mRNA catabolic process / Protein hydroxylation / Peptide chain elongation / Selenocysteine synthesis / Formation of a pool of free 40S subunits / cellular response to actinomycin D / DNA replication initiation / Eukaryotic Translation Termination / blastocyst development / negative regulation of ubiquitin-dependent protein catabolic process / SRP-dependent cotranslational protein targeting to membrane / Response of EIF2AK4 (GCN2) to amino acid deficiency / ubiquitin ligase inhibitor activity / Viral mRNA Translation / positive regulation of signal transduction by p53 class mediator / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / GTP hydrolysis and joining of the 60S ribosomal subunit / ribonucleoprotein complex binding / L13a-mediated translational silencing of Ceruloplasmin expression / hematopoietic progenitor cell differentiation / RNA processing / Major pathway of rRNA processing in the nucleolus and cytosol / nucleosome binding / ribosomal subunit export from nucleus / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / maturation of LSU-rRNA / estrogen receptor signaling pathway / Notch signaling pathway / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rough endoplasmic reticulum / translation initiation factor activity / MDM2/MDM4 family protein binding / negative regulation of protein ubiquitination / transcription initiation-coupled chromatin remodeling / cytosolic ribosome / cellular response to interleukin-4 / nuclear periphery

Domain/homology:

WD repeat protein 55 / WDR55 / : / P120R repeat / NOP2 repeat / DBP10, C-terminal / DDX54/DBP10 family / DBP10CT (NUC160) domain / DBP10CT (NUC160) domain / : / Methyltr_RsmF/B-like, ferredoxin-like domain / Nucleolar complex protein 2 / Noc2p family / Nucleolar complex-associated protein 3, N-terminal / Nucleolar complex-associated protein 3 / Nucleolar complex-associated protein / WD repeat WDR12/Ytm1 / MKI67 FHA domain-interacting nucleolar phosphoprotein, FHA Ki67 binding / FHA Ki67 binding domain of hNIFK / Ribosome biogenesis factor, NIP7 / RNA (C5-cytosine) methyltransferase, NOP2 / : / UPF0113, pre-PUA domain / UPF0113 PUA domain / NIP7/UPF0113, pre-PUA domain / UPF0113, PUA domain / Nop2p / Bacterial Fmu (Sun)/eukaryotic nucleolar NOL1/Nop2p, conserved site / NOL1/NOP2/sun family signature. / Learning associated protein 18-like / LLP homolog / Ribosomal RNA methyltransferase, SPB1-like, C-terminal / Ribosomal RNA methyltransferase Spb1, domain of unknown function DUF3381 / AdoMet-dependent rRNA methyltransferase SPB1-like / Spb1 C-terminal domain / Ribosomal RNA methyltransferase Spb1, DUF3381 / : / Ribosomal biogenesis regulatory protein / Ribosome biogenesis regulatory protein (RRS1) / Eukaryotic rRNA processing / Eukaryotic rRNA processing protein EBP2 / : / Ribosomal RNA large subunit methyltransferase E / Ribosome biogenesis protein BRX1 / DDX18/Has1, DEAD-box helicase domain / Ribosome biogenesis protein Nop16 / Ribosome biogenesis protein Nop16 / Domain of unknown function DUF4217 / ATP-dependent rRNA helicase SPB4-like, C-terminal extension / DUF4217 / BOP1, N-terminal domain / WD repeat BOP1/Erb1 / BOP1NT (NUC169) domain / BOP1NT (NUC169) domain / NLE / NLE (NUC135) domain / SAM-dependent methyltransferase RsmB/NOP2-type / RNA (C5-cytosine) methyltransferase / : / 16S rRNA methyltransferase RsmB/F / SAM-dependent MTase RsmB/NOP-type domain profile. / Guanine nucleotide-binding protein-like 3, N-terminal domain / GNL3L/Grn1 putative GTPase / CCAAT-binding factor / CBF/Mak21 family / Putative RNA-binding Domain in PseudoUridine synthase and Archaeosine transglycosylase / PUA domain / PUA domain superfamily / : / PUA domain profile. / Pescadillo / Pescadillo N-terminus / Ribosomal biogenesis NSA2 family / : / Ribosome assembly factor Mrt4 / : / BRCT domain / NOG, C-terminal / Nucleolar GTP-binding protein 1 / NOGCT (NUC087) domain / Nucleolar GTP-binding protein 1, Rossman-fold domain / NOG1, N-terminal helical domain / Nucleolar GTP-binding protein 1 (NOG1) / NOG1 N-terminal helical domain / Circularly permuted (CP)-type guanine nucleotide-binding (G) domain / Circularly permuted (CP)-type guanine nucleotide-binding (G) domain profile. / Brix domain / Brix domain / Brix domain profile. / Brix / OBG-type guanine nucleotide-binding (G) domain / OBG-type guanine nucleotide-binding (G) domain profile. / Translation initiation factor IF6 / eIF-6 family / translation initiation factor 6 / DEAD-box subfamily ATP-dependent helicases signature. / ATP-dependent RNA helicase DEAD-box, conserved site / 50S ribosome-binding GTPase / RNA helicase, DEAD-box type, Q motif / DEAD-box RNA helicase Q motif profile.

Component:

Pescadillo homolog / Ribosomal L1 domain-containing protein 1 / Ribosome biogenesis protein NSA2 homolog / Large ribosomal subunit protein eL33 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein eL13 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL29 / 28S rRNA (cytosine(4447)-C(5))-methyltransferase / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein eL28 / Large ribosomal subunit protein eL34 / Large ribosomal subunit protein eL14 / Eukaryotic translation initiation factor 6 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein eL15 / Large ribosomal subunit protein eL27 / Large ribosomal subunit protein eL37 / Large ribosomal subunit protein eL8 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein uL1 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein eL19 / Large ribosomal subunit protein eL20 / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein eL18 / Ribosome biogenesis protein BOP1 / Ribosome biogenesis regulatory protein homolog / Ribosomal protein uL30-like / pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3 / ATP-dependent RNA helicase DDX54 / Ribosome biogenesis protein BRX1 homolog / Nucleolar complex protein 3 homolog / Probable rRNA-processing protein EBP2 / Protein LLP homolog / Guanine nucleotide-binding protein-like 3 / MKI67 FHA domain-interacting nucleolar phosphoprotein / GTP-binding protein 4 / Ribosome biogenesis protein WDR12 / WD repeat-containing protein 55 / ATP-dependent RNA helicase DDX18 / Probable ribosome biogenesis protein RLP24 / mRNA turnover protein 4 homolog / 60S ribosome subunit biogenesis protein NIP7 homolog / Nucleolar protein 16 / Nucleolar complex protein 2 homolog / Large ribosomal subunit protein eL36

• Biological species: Homo sapiens (human) / human (human)
• Method: single particle reconstruction / cryo EM / Resolution: 2.67 Å
• Authors: Vanden Broeck A / Klinge S

Funding support

European Union, United States, 2 items

Organization	Grant number	Country
European Molecular Biology Organization (EMBO)	ALTF 711-2019	European Union
The G. Harold and Leila Y. Mathers Foundation	MF-2104-01554	United States

• Citation: Journal: Science / Year: 2023; Title: Principles of human pre-60 biogenesis.; Authors: Arnaud Vanden Broeck / Sebastian Klinge / ; Abstract: During the early stages of human large ribosomal subunit (60) biogenesis, an ensemble of assembly factors establishes and fine-tunes the essential RNA functional centers of pre-60 particles by an unknown mechanism. Here, we report a series of cryo-electron microscopy structures of human nucleolar and nuclear pre-60 assembly intermediates at resolutions of 2.5 to 3.2 angstroms. These structures show how protein interaction hubs tether assembly factor complexes to nucleolar particles and how guanosine triphosphatases and adenosine triphosphatase couple irreversible nucleotide hydrolysis steps to the installation of functional centers. Nuclear stages highlight how a conserved RNA-processing complex, the rixosome, couples large-scale RNA conformational changes with pre-ribosomal RNA processing by the RNA degradation machinery. Our ensemble of human pre-60 particles provides a rich foundation with which to elucidate the molecular principles of ribosome formation.

History

Deposition	Dec 21, 2022	-
Header (metadata) release	Jul 12, 2023	-
Map release	Jul 12, 2023	-
Update	Jul 19, 2023	-
Current status	Jul 19, 2023	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_29261.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.072 Å

Density

Contour Level	By AUTHOR: 0.85
Minimum - Maximum	0.0 - 9.161265999999999
Average (Standard dev.)	0.05099254 (±0.19183522)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 480 480 480 Spacing 480 480 480
Cell	A=B=C: 514.56 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_29261_msk_1.map

Half map: #2

• File: emd_29261_half_map_1.map

Half map: #1

• File: emd_29261_half_map_2.map

Sample components

Entire : Human nucleolar pre-60S ribosomal subunit (State F)

• Entire: Name: Human nucleolar pre-60S ribosomal subunit (State F)

Components

• Complex: Human nucleolar pre-60S ribosomal subunit (State F)
  • Protein or peptide: 60S ribosomal protein L12
  • Protein or peptide: 60S ribosomal protein L10a
  • Protein or peptide: WD repeat-containing protein 55
  • RNA: 5.8S rRNA
  • RNA: ITS2 rRNA
  • RNA: 28S rRNA
  • Protein or peptide: 60S ribosomal protein L13
  • Protein or peptide: 60S ribosomal protein L13a
  • Protein or peptide: 60S ribosomal protein L14
  • Protein or peptide: 60S ribosomal protein L15
  • Protein or peptide: 60S ribosomal protein L17
  • Protein or peptide: 60S ribosomal protein L18
  • Protein or peptide: 60S ribosomal protein L18a
  • Protein or peptide: 60S ribosomal protein L19
  • Protein or peptide: 60S ribosomal protein L21
  • Protein or peptide: 60S ribosomal protein L23
  • Protein or peptide: 60S ribosomal protein L23a
  • Protein or peptide: 60S ribosomal protein L26
  • Protein or peptide: 60S ribosomal protein L27
  • Protein or peptide: 60S ribosomal protein L27a
  • Protein or peptide: 60S ribosomal protein L28
  • Protein or peptide: 60S ribosomal protein L3
  • Protein or peptide: 60S ribosomal protein L30
  • Protein or peptide: 60S ribosomal protein L31
  • Protein or peptide: 60S ribosomal protein L32
  • Protein or peptide: 60S ribosomal protein L34
  • Protein or peptide: 60S ribosomal protein L35
  • Protein or peptide: 60S ribosomal protein L35a
  • Protein or peptide: 60S ribosomal protein L36
  • Protein or peptide: 60S ribosomal protein L37
  • Protein or peptide: Nucleolar complex protein 2 homolog
  • Protein or peptide: Guanine nucleotide-binding protein-like 3
  • Protein or peptide: Ribosome biogenesis protein NSA2 homolog
  • Protein or peptide: 60S ribosome subunit biogenesis protein NIP7 homolog
  • Protein or peptide: ATP-dependent RNA helicase DDX54
  • Protein or peptide: Protein LLP homolog
  • Protein or peptide: 60S ribosomal protein L4
  • Protein or peptide: 60S ribosomal protein L6
  • Protein or peptide: 60S ribosomal protein L7
  • Protein or peptide: 60S ribosomal protein L7a
  • Protein or peptide: 60S ribosomal protein L9
  • Protein or peptide: MKI67 FHA domain-interacting nucleolar phosphoprotein
  • Protein or peptide: 60S ribosomal protein L7-like 1
  • Protein or peptide: pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3
  • Protein or peptide: Eukaryotic translation initiation factor 6
  • Protein or peptide: Ribosomal L1 domain-containing protein 1
  • Protein or peptide: Pescadillo homolog
  • Protein or peptide: Probable rRNA-processing protein EBP2
  • Protein or peptide: Ribosome biogenesis protein BRX1 homolog
  • Protein or peptide: Ribosome biogenesis protein WDR12
  • Protein or peptide: mRNA turnover protein 4 homolog
  • Protein or peptide: GTP-binding protein 4
  • Protein or peptide: Ribosome biogenesis protein BOP1
  • Protein or peptide: Ribosome biogenesis regulatory protein homolog
  • Protein or peptide: Nucleolar complex protein 3 homolog
  • Protein or peptide: Probable ribosome biogenesis protein RLP24
  • Protein or peptide: ATP-dependent RNA helicase DDX18
  • Protein or peptide: Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase
  • Protein or peptide: Nucleolar protein 16
• Ligand: MAGNESIUM ION
• Ligand: ZINC ION
• Ligand: ADENOSINE-5'-DIPHOSPHATE
• Ligand: GUANOSINE-5'-DIPHOSPHATE
• Ligand: POTASSIUM ION

Supramolecule #1: Human nucleolar pre-60S ribosomal subunit (State F)

• Supramolecule: Name: Human nucleolar pre-60S ribosomal subunit (State F) / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#59
• Source (natural): Organism: Homo sapiens (human) / Strain: HEK293F

Macromolecule #1: 60S ribosomal protein L12

• Macromolecule: Name: 60S ribosomal protein L12 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 17.847619 KDa

Sequence

String:

MPPKFDPNEI KVVYLRCTGG EVGATSALAP KIGPLGLSPK KVGDDIAKAT GDWKGLRITV KLTIQNRQAQ IEVVPSASAL IIKALKEPP RDRKKQKNIK HSGNITFDEI VNIARQMRHR SLARELSGTI KEILGTAQSV GCNVDGRHPH DIIDDINSGA V ECPAS

UniProtKB: Large ribosomal subunit protein uL11

Macromolecule #2: 60S ribosomal protein L10a

• Macromolecule: Name: 60S ribosomal protein L10a / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 24.879422 KDa

Sequence

String:

MSSKVSRDTL YEAVREVLHG NQRKRRKFLE TVELQISLKN YDPQKDKRFS GTVRLKSTPR PKFSVCVLGD QQHCDEAKAV DIPHMDIEA LKKLNKNKKL VKKLAKKYDA FLASESLIKQ IPRILGPGLN KAGKFPSLLT HNENMVAKVD EVKSTIKFQM K KVLCLAVA VGHVKMTDDE LVYNIHLAVN FLVSLLKKNW QNVRALYIKS TMGKPQRLY

UniProtKB: Large ribosomal subunit protein uL1

Macromolecule #3: WD repeat-containing protein 55

• Macromolecule: Name: WD repeat-containing protein 55 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 42.123012 KDa

Sequence

String:

MDRTCEERPA EDGSDEEDPD SMEAPTRIRD TPEDIVLEAP ASGLAFHPAR DLLAAGDVDG DVFVFSYSCQ EGETKELWSS GHHLKACRA VAFSEDGQKL ITVSKDKAIH VLDVEQGQLE RRVSKAHGAP INSLLLVDEN VLATGDDTGG ICLWDQRKEG P LMDMRQHE EYIADMALDP AKKLLLTASG DGCLGIFNIK RRRFELLSEP QSGDLTSVTL MKWGKKVACG SSEGTIYLFN WN GFGATSD RFALRAESID CMVPVTESLL CTGSTDGVIR AVNILPNRVV GSVGQHTGEP VEELALSHCG RFLASSGHDQ RLK FWDMAQ LRAVVVDDYR RRKKKGGPLR ALSSKTWSTD DFFAGLREEG EDSMAQEEKE ETGDDSD

UniProtKB: WD repeat-containing protein 55

Macromolecule #7: 60S ribosomal protein L13

• Macromolecule: Name: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 24.321682 KDa

Sequence

String:

MAPSRNGMVL KPHFHKDWQR RVATWFNQPA RKIRRRKARQ AKARRIAPRP ASGPIRPIVR CPTVRYHTKV RAGRGFSLEE LRVAGIHKK VARTIGISVD PRRRNKSTES LQANVQRLKE YRSKLILFPR KPSAPKKGDS SAEELKLATQ LTGPVMPVRN V YKKEKARV ITEEEKNFKA FASLRMARAN ARLFGIRAKR AKEAAEQDVE KKK

UniProtKB: Large ribosomal subunit protein eL13

Macromolecule #8: 60S ribosomal protein L13a

• Macromolecule: Name: 60S ribosomal protein L13a / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 23.633412 KDa

Sequence

String:

MAEVQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLP HKTKRGQAAL DRLKVFDGIP PPYDKKKRMV VPAALKVVRL KPTRKFAYLG RLAHEVGWKY QAVTATLEEK R KEKAKIHY RKKKQLMRLR KQAEKNVEKK IDKYTEVLKT HGLLV

UniProtKB: Large ribosomal subunit protein uL13

Macromolecule #9: 60S ribosomal protein L14

• Macromolecule: Name: 60S ribosomal protein L14 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 23.485016 KDa

Sequence

String:

MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTQVRRQAM PFKCMQLTDF ILKFPHSAHQ KYVRQAWQKA DINTKWAAT RWAKKIEARE RKAKMTDFDR FKVMKAKKMR NRIIKNEVKK LQKAALLKAS PKKAPGTKGT AAAAAAAAAA K VPAKKITA ASKKAPAQKV PAQKATGQKA APAPKAQKGQ KAPAQKAPAP KASGKKA

UniProtKB: Large ribosomal subunit protein eL14

Macromolecule #10: 60S ribosomal protein L15

• Macromolecule: Name: 60S ribosomal protein L15 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 24.207285 KDa

Sequence

String:

MGAYKYIQEL WRKKQSDVMR FLLRVRCWQY RQLSALHRAP RPTRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGAT YGKPVHHGV NQLKFARSLQ SVAEERAGRH CGALRVLNSY WVGEDSTYKF FEVILIDPFH KAIRRNPDTQ WITKPVHKHR E MRGLTSAG RKSRGLGKGH KFHHTIGGSR RAAWRRRNTL QLHRYR

UniProtKB: Large ribosomal subunit protein eL15

Macromolecule #11: 60S ribosomal protein L17

• Macromolecule: Name: 60S ribosomal protein L17 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 21.44317 KDa

Sequence

String:

MVRYSLDPEN PTKSCKSRGS NLRVHFKNTR ETAQAIKGMH IRKATKYLKD VTLQKQCVPF RRYNGGVGRC AQAKQWGWTQ GRWPKKSAE FLLHMLKNAE SNAELKGLDV DSLVIEHIQV NKAPKMRRRT YRAHGRINPY MSSPCHIEMI LTEKEQIVPK P EEEVAQKK KISQKKLKKQ KLMARE

UniProtKB: Large ribosomal subunit protein uL22

Macromolecule #12: 60S ribosomal protein L18

• Macromolecule: Name: 60S ribosomal protein L18 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 21.687676 KDa

Sequence

String:

MGVDIRHNKD RKVRRKEPKS QDIYLRLLVK LYRFLARRTN STFNQVVLKR LFMSRTNRPP LSLSRMIRKM KLPGRENKTA VVVGTITDD VRVQEVPKLK VCALRVTSRA RSRILRAGGK ILTFDQLALD SPKGCGTVLL SGPRKGREVY RHFGKAPGTP H SHTKPYVR SKGRKFERAR GRRASRGYKN

UniProtKB: Large ribosomal subunit protein eL18

Macromolecule #13: 60S ribosomal protein L18a

• Macromolecule: Name: 60S ribosomal protein L18a / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 20.808514 KDa

Sequence

String:

MKASGTLREY KVVGRCLPTP KCHTPPLYRM RIFAPNHVVA KSRFWYFVSQ LKKMKKSSGE IVYCGQVFEK SPLRVKNFGI WLRYDSRSG THNMYREYRD LTTAGAVTQC YRDMGARHRA RAHSIQIMKV EEIAASKCRR PAVKQFHDSK IKFPLPHRVL R RQHKPRFT TKRPNTFF

UniProtKB: Large ribosomal subunit protein eL20

Macromolecule #14: 60S ribosomal protein L19

• Macromolecule: Name: 60S ribosomal protein L19 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 23.535281 KDa

Sequence

String:

MSMLRLQKRL ASSVLRCGKK KVWLDPNETN EIANANSRQQ IRKLIKDGLI IRKPVTVHSR ARCRKNTLAR RKGRHMGIGK RKGTANARM PEKVTWMRRM RILRRLLRRY RESKKIDRHM YHSLYLKVKG NVFKNKRILM EHIHKLKADK ARKKLLADQA E ARRSKTKE ARKRREERLQ AKKEEIIKTL SKEEETKK

UniProtKB: Large ribosomal subunit protein eL19

Macromolecule #15: 60S ribosomal protein L21

• Macromolecule: Name: 60S ribosomal protein L21 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 18.609988 KDa

Sequence

String:

MTNTKGKRRG TRYMFSRPFR KHGVVPLATY MRIYKKGDIV DIKGMGTVQK GMPHKCYHGK TGRVYNVTQH AVGIVVNKQV KGKILAKRI NVRIEHIKHS KSRDSFLKRV KENDQKKKEA KEKGTWVQLK RQPAPPREAH FVRTNGKEPE LLEPIPYEFM A

UniProtKB: Large ribosomal subunit protein eL21

Macromolecule #16: 60S ribosomal protein L23

• Macromolecule: Name: 60S ribosomal protein L23 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 14.892505 KDa

Sequence

String:

MSKRGRGGSS GAKFRISLGL PVGAVINCAD NTGAKNLYII SVKGIKGRLN RLPAAGVGDM VMATVKKGKP ELRKKVHPAV VIRQRKSYR RKDGVFLYFE DNAGVIVNNK GEMKGSAITG PVAKECADLW PRIASNAGSI A

UniProtKB: Large ribosomal subunit protein uL14

Macromolecule #17: 60S ribosomal protein L23a

• Macromolecule: Name: 60S ribosomal protein L23a / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 17.740193 KDa

Sequence

String:

MAPKAKKEAP APPKAEAKAK ALKAKKAVLK GVHSHKKKKI RTSPTFRRPK TLRLRRQPKY PRKSAPRRNK LDHYAIIKFP LTTESAMKK IEDNNTLVFI VDVKANKHQI KQAVKKLYDI DVAKVNTLIR PDGEKKAYVR LAPDYDALDV ANKIGII

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #18: 60S ribosomal protein L26

• Macromolecule: Name: 60S ribosomal protein L26 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 17.303363 KDa

Sequence

String:

MKFNPFVTSD RSKNRKRHFN APSHIRRKIM SSPLSKELRQ KYNVRSMPIR KDDEVQVVRG HYKGQQIGKV VQVYRKKYVI YIERVQREK ANGTTVHVGI HPSKVVITRL KLDKDRKKIL ERKAKSRQVG KEKGKYKEET IEKMQE

UniProtKB: Large ribosomal subunit protein uL24

Macromolecule #19: 60S ribosomal protein L27

• Macromolecule: Name: 60S ribosomal protein L27 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 15.835831 KDa

Sequence

String:

MGKFMKPGKV VLVLAGRYSG RKAVIVKNID DGTSDRPYSH ALVAGIDRYP RKVTAAMGKK KIAKRSKIKS FVKVYNYNHL MPTRYSVDI PLDKTVVNKD VFRDPALKRK ARREAKVKFE ERYKTGKNKW FFQKLRF

UniProtKB: Large ribosomal subunit protein eL27

Macromolecule #20: 60S ribosomal protein L27a

• Macromolecule: Name: 60S ribosomal protein L27a / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 16.604535 KDa

Sequence

String:

MPSRLRKTRK LRGHVSHGHG RIGKHRKHPG GRGNAGGLHH HRINFDKYHP GYFGKVGMKH YHLKRNQSFC PTVNLDKLWT LVSEQTRVN AAKNKTGAAP IIDVVRSGYY KVLGKGKLPK QPVIVKAKFF SRRAEEKIKS VGGACVLVA

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #21: 60S ribosomal protein L28

• Macromolecule: Name: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 15.784622 KDa

Sequence

String:

MSAHLQWMVV RNCSSFLIKR NKQTYSTEPN NLKARNSFRY NGLIHRKTVG VEPAADGKGV VVVIKRRSGQ RKPATSYVRT TINKNARAT LSSIRHMIRK NKYRPDLRMA AIRRASAILR SQKPVMVKRK RTRPTKSS

UniProtKB: Large ribosomal subunit protein eL28

Macromolecule #22: 60S ribosomal protein L3

• Macromolecule: Name: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 46.224133 KDa

Sequence

String:

MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD EDGKKQLEKD FSSMKKYCQV I RVIAHTQM RLLPLRQKKA HLMEIQVNGG TVAEKLDWAR ERLEQQVPVN QVFGQDEMID VIGVTKGKGY KGVTSRWHTK KL PRKT(HIC)RG LRKVACIGAW HPARVAFSVA RAGQKGYHHR TEINKKIYKI GQGYLIKDGK LIKNNASTDY DLSDKSINP LGGFVHYGEV TNDFVMLKGC VVGTKKRVLT LRKSLLVQTK RRALEKIDLK FIDTTSKFGH GRFQTMEEKK AFMGPLKKDR IAKEEGA

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #23: 60S ribosomal protein L30

• Macromolecule: Name: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 12.805092 KDa

Sequence

String:

MVAAKKTKKS LESINSRLQL VMKSGKYVLG YKQTLKMIRQ GKAKLVILAN NCPALRKSEI EYYAMLAKTG VHHYSGNNIE LGTACGKYY RVCTLAIIDP GDSDIIRSMP EQTGEK

UniProtKB: Large ribosomal subunit protein eL30

Macromolecule #24: 60S ribosomal protein L31

• Macromolecule: Name: 60S ribosomal protein L31 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 14.494938 KDa

Sequence

String:

MAPAKKGGEK KKGRSAINEV VTREYTINIH KRIHGVGFKK RAPRALKEIR KFAMKEMGTP DVRIDTRLNK AVWAKGIRNV PYRIRVRLS RKRNEDEDSP NKLYTLVTYV PVTTFKNLQT VNVDEN

UniProtKB: Large ribosomal subunit protein eL31

Macromolecule #25: 60S ribosomal protein L32

• Macromolecule: Name: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 15.898932 KDa

Sequence

String:

MAALRPLVKP KIVKKRTKKF IRHQSDRYVK IKRNWRKPRG IDNRVRRRFK GQILMPNIGY GSNKKTKHML PSGFRKFLVH NVKELEVLL MCNKSYCAEI AHNVSSKNRK AIVERAAQLA IRVTNPNARL RSEENE

UniProtKB: Large ribosomal subunit protein eL32

Macromolecule #26: 60S ribosomal protein L34

• Macromolecule: Name: 60S ribosomal protein L34 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 13.326074 KDa

Sequence

String:

MVQRLTYRRR LSYNTASNKT RLSRTPGNRI VYLYTKKVGK APKSACGVCP GRLRGVRAVR PKVLMRLSKT KKHVSRAYGG SMCAKCVRD RIKRAFLIEE QKIVVKVLKA QAQSQKAK

UniProtKB: Large ribosomal subunit protein eL34

Macromolecule #27: 60S ribosomal protein L35

• Macromolecule: Name: 60S ribosomal protein L35 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 14.593624 KDa

Sequence

String:

MAKIKARDLR GKKKEELLKQ LDDLKVELSQ LRVAKVTGGA ASKLSKIRVV RKSIARVLTV INQTQKENLR KFYKGKKYKP LDLRPKKTR AMRRRLNKHE ENLKTKKQQR KERLYPLRKY AVKA

UniProtKB: Large ribosomal subunit protein uL29

Macromolecule #28: 60S ribosomal protein L35a

• Macromolecule: Name: 60S ribosomal protein L35a / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 12.564743 KDa

Sequence

String:

MSGRLWSKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV YKAKNNTVTP GGKPNKTRVI WGKVTRAHGN SGMVRAKFR SNLPAKAIGH RIRVMLYPSR I

UniProtKB: Large ribosomal subunit protein eL33

Macromolecule #29: 60S ribosomal protein L36

• Macromolecule: Name: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 12.290859 KDa

Sequence

String:

MALRYPMAVG LNKGHKVTKN VSKPRHSRRR GRLTKHTKFV RDMIREVCGF APYERRAMEL LKVSKDKRAL KFIKKRVGTH IRAKRKREE LSNVLAAMRK AAAKKD

UniProtKB: Large ribosomal subunit protein eL36

Macromolecule #30: 60S ribosomal protein L37

• Macromolecule: Name: 60S ribosomal protein L37 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 11.111032 KDa

Sequence

String:

MTKGTSSFGK RRNKTHTLCR RCGSKAYHLQ KSTCGKCGYP AKRKRKYNWS AKAKRRNTTG TGRMRHLKIV YRRFRHGFRE GTTPKPKRA AVAASSSS

UniProtKB: Large ribosomal subunit protein eL37

Macromolecule #31: Nucleolar complex protein 2 homolog

• Macromolecule: Name: Nucleolar complex protein 2 homolog / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 85.028359 KDa

Sequence

String:

MAAAGSRKRR LAELTVDEFL ASGFDSESES ESENSPQAET REAREAARSP DKPGGSPSAS RRKGRASEHK DQLSRLKDRD PEFYKFLQE NDQSLLNFSD SDSSEEEEGP FHSLPDVLEE ASEEEDGAEE GEDGDRVPRG LKGKKNSVPV TVAMVERWKQ A AKQRLTPK LFHEVVQAFR AAVATTRGDQ ESAEANKFQV TDSAAFNALV TFCIRDLIGC LQKLLFGKVA KDSSRMLQPS SS PLWGKLR VDIKAYLGSA IQLVSCLSET TVLAAVLRHI SVLVPCFLTF PKQCRMLLKR MVIVWSTGEE SLRVLAFLVL SRV CRHKKD TFLGPVLKQM YITYVRNCKF TSPGALPFIS FMQWTLTELL ALEPGVAYQH AFLYIRQLAI HLRNAMTTRK KETY QSVYN WQYVHCLFLW CRVLSTAGPS EALQPLVYPL AQVIIGCIKL IPTARFYPLR MHCIRALTLL SGSSGAFIPV LPFIL EMFQ QVDFNRKPGR MSSKPINFSV ILKLSNVNLQ EKAYRDGLVE QLYDLTLEYL HSQAHCIGFP ELVLPVVLQL KSFLRE CKV ANYCRQVQQL LGKVQENSAY ICSRRQRVSF GVSEQQAVEA WEKLTREEGT PLTLYYSHWR KLRDREIQLE ISGKERL ED LNFPEIKRRK MADRKDEDRK QFKDLFDLNS SEEDDTEGFS ERGILRPLST RHGVEDDEED EEEGEEDSSN SEDGDPDA E AGLAPGELQQ LAQGPEDELE DLQLSEDD

UniProtKB: Nucleolar complex protein 2 homolog

Macromolecule #32: Guanine nucleotide-binding protein-like 3

• Macromolecule: Name: Guanine nucleotide-binding protein-like 3 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 62.098242 KDa

Sequence

String:

MKRPKLKKAS KRMTCHKRYK IQKKVREHHR KLRKEAKKRG HKKPRKDPGV PNSAPFKEAL LREAELRKQR LEELKQQQKL DRQKELEKK RKLETNPDIK PSNVEPMEKE FGLCKTENKA KSGKQNSKKL YCQELKKVIE ASDVVLEVLD ARDPLGCRCP Q VEEAIVQS GQKKLVLILN KSDLVPKENL ESWLNYLKKE LPTVVFRAST KPKDKGKITK RVKAKKNAAP FRSEVCFGKE GL WKLLGGF QETCSKAIRV GVIGFPNVGK SSIINSLKQE QMCNVGVSMG LTRSMQVVPL DKQITIIDSP SFIVSPLNSS SAL ALRSPA SIEVVKPMEA ASAILSQADA RQVVLKYTVP GYRNSLEFFT VLAQRRGMHQ KGGIPNVEGA AKLLWSEWTG ASLA YYCHP PTSWTPPPYF NESIVVDMKS GFNLEELEKN NAQSIRAIKG PHLANSILFQ SSGLTNGIIE EKDIHEELPK RKERK QEER EDDKDSDQET VDEEVDENSS GMFAAEETGE ALSEETTAGE QSTRSFILDK IIEEDDAYDF STDYV

UniProtKB: Guanine nucleotide-binding protein-like 3

Macromolecule #33: Ribosome biogenesis protein NSA2 homolog

• Macromolecule: Name: Ribosome biogenesis protein NSA2 homolog / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 30.136703 KDa

Sequence

String:

MPQNEYIELH RKRYGYRLDY HEKKRKKESR EAHERSKKAK KMIGLKAKLY HKQRHAEKIQ MKKTIKMHEK RNTKQKNDEK TPQGAVPAY LLDREGQSRA KVLSNMIKQK RKEKAGKWEV PLPKVRAQGE TEVLKVIRTG KRKKKAWKRM VTKVCFVGDG F TRKPPKYE RFIRPMGLRF KKAHVTHPEL KATFCLPILG VKKNPSSPLY TTLGVITKGT VIEVNVSELG LVTQGGKVIW GK YAQVTNN PENDGCINAV LLV

UniProtKB: Ribosome biogenesis protein NSA2 homolog

Macromolecule #34: 60S ribosome subunit biogenesis protein NIP7 homolog

• Macromolecule: Name: 60S ribosome subunit biogenesis protein NIP7 homolog / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 20.491633 KDa

Sequence

String:

MRPLTEEETR VMFEKIAKYI GENLQLLVDR PDGTYCFRLH NDRVYYVSEK IMKLAANISG DKLVSLGTCF GKFTKTHKFR LHVTALDYL APYAKYKVWI KPGAEQSFLY GNHVLKSGLG RITENTSQYQ GVVVYSMADI PLGFGVAAKS TQDCRKVDPM A IVVFHQAD IGEYVRHEET LT

UniProtKB: 60S ribosome subunit biogenesis protein NIP7 homolog

Macromolecule #35: ATP-dependent RNA helicase DDX54

• Macromolecule: Name: ATP-dependent RNA helicase DDX54 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 98.769164 KDa

Sequence

String:

MAADKGPAAG PRSRAAMAQW RKKKGLRKRR GAASQARGSD SEDGEFEIQA EDDARARKLG PGRPLPTFPT SECTSDVEPD TREMVRAQN KKKKKSGGFQ SMGLSYPVFK GIMKKGYKVP TPIQRKTIPV ILDGKDVVAM ARTGSGKTAC FLLPMFERLK T HSAQTGAR ALILSPTREL ALQTLKFTKE LGKFTGLKTA LILGGDRMED QFAALHENPD IIIATPGRLV HVAVEMSLKL QS VEYVVFD EADRLFEMGF AEQLQEIIAR LPGGHQTVLF SATLPKLLVE FARAGLTEPV LIRLDVDTKL NEQLKTSFFL VRE DTKAAV LLHLLHNVVR PQDQTVVFVA TKHHAEYLTE LLTTQRVSCA HIYSALDPTA RKINLAKFTL GKCSTLIVTD LAAR GLDIP LLDNVINYSF PAKGKLFLHR VGRVARAGRS GTAYSLVAPD EIPYLLDLHL FLGRSLTLAR PLKEPSGVAG VDGML GRVP QSVVDEEDSG LQSTLEASLE LRGLARVADN AQQQYVRSRP APSPESIKRA KEMDLVGLGL HPLFSSRFEE EELQRL RLV DSIKNYRSRA TIFEINASSR DLCSQVMRAK RQKDRKAIAR FQQGQQGRQE QQEGPVGPAP SRPALQEKQP EKEEEEE AG ESVEDIFSEV VGRKRQRSGP NRGAKRRREE ARQRDQEFYI PYRPKDFDSE RGLSISGEGG AFEQQAAGAV LDLMGDEA Q NLTRGRQQLK WDRKKKRFVG QSGQEDKKKI KTESGRYISS SYKRDLYQKW KQKQKIDDRD SDEEGASDRR GPERRGGKR DRGQGASRPH APGTPAGRVR PELKTKQQIL KQRRRAQKLH FLQRGGLKQL SARNRRRVQE LQQGAFGRGA RSKKGKMRKR M

UniProtKB: ATP-dependent RNA helicase DDX54

Macromolecule #36: Protein LLP homolog

• Macromolecule: Name: Protein LLP homolog / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 15.268361 KDa

Sequence

String:

MAKSLRSKWK RKMRAEKRKK NAPKEASRLK SILKLDGDVL MKDVQEIATV VVPKPKHCQE KMQCEVKDEK DDMKMETDIK RNKKTLLDQ HGQYPIWMNQ RQRKRLKAKR EKRKGKSKAK AVKVAKGLAW

UniProtKB: Protein LLP homolog

Macromolecule #37: 60S ribosomal protein L4

• Macromolecule: Name: 60S ribosomal protein L4 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 47.804621 KDa

Sequence

String:

MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVEDKVE G YKKTKEAV LLLKKLKAWN DIKKVYASQR MRAGKGKMRN RRRIQRRGPC IIYNEDNGII KAFRNIPGIT LLNVSKLNIL KL APGGHVG RFCIWTESAF RKLDELYGTW RKAASLKSNY NLPMHKMINT DLSRILKSPE IQRALRAPRK KIHRRVLKKN PLK NLRIML KLNPYAKTMR RNTILRQARN HKLRVDKAAA AAAALQAKSD EKAAVAGKKP VVGKKGKKAA VGVKKQKKPL VGKK AAATK KPAPEKKPAE KKPTTEEKKP AA

UniProtKB: Large ribosomal subunit protein uL4

Macromolecule #38: 60S ribosomal protein L6

• Macromolecule: Name: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 32.810176 KDa

Sequence

String:

MAGEKVEKPD TKEKKPEAKK VDAGGKVKKG NLKAKKPKKG KPHCSRNPVL VRGIGRYSRS AMYSRKAMYK RKYSAAKSKV EKKKKEKVL ATVTKPVGGD KNGGTRVVKL RKMPRYYPTE DVPRKLLSHG KKPFSQHVRK LRASITPGTI LIILTGRHRG K RVVFLKQL ASGLLLVTGP LVLNRVPLRR THQKFVIATS TKIDISNVKI PKHLTDAYFK KKKLRKPRHQ EGEIFDTEKE KY EITEQRK IDQKAVDSQI LPKIKAIPQL QGYLRSVFAL TNGIYPHKLV F

UniProtKB: Large ribosomal subunit protein eL6

Macromolecule #39: 60S ribosomal protein L7

• Macromolecule: Name: 60S ribosomal protein L7 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 29.290973 KDa

Sequence

String:

MEGVEEKKKE VPAVPETLKK KRRNFAELKI KRLRKKFAQK MLRKARRKLI YEKAKHYHKE YRQMYRTEIR MARMARKAGN FYVPAEPKL AFVIRIRGIN GVSPKVRKVL QLLRLRQIFN GTFVKLNKAS INMLRIVEPY IAWGYPNLKS VNELIYKRGY G KINKKRIA LTDNALIARS LGKYGIICME DLIHEIYTVG KRFKEANNFL WPFKLSSPRG GMKKKTTHFV EGGDAGNRED QI NRLIRRM N

UniProtKB: Large ribosomal subunit protein uL30

Macromolecule #40: 60S ribosomal protein L7a

• Macromolecule: Name: 60S ribosomal protein L7a / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 30.061785 KDa

Sequence

String:

MPKGKKAKGK KVAPAPAVVK KQEAKKVVNP LFEKRPKNFG IGQDIQPKRD LTRFVKWPRY IRLQRQRAIL YKRLKVPPAI NQFTQALDR QTATQLLKLA HKYRPETKQE KKQRLLARAE KKAAGKGDVP TKRPPVLRAG VNTVTTLVEN KKAQLVVIAH D VDPIELVV FLPALCRKMG VPYCIIKGKA RLGRLVHRKT CTTVAFTQVN SEDKGALAKL VEAIRTNYND RYDEIRRHWG GN VLGPKSV ARIAKLEKAK AKELATKLG

UniProtKB: Large ribosomal subunit protein eL8

Macromolecule #41: 60S ribosomal protein L9

• Macromolecule: Name: 60S ribosomal protein L9 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 21.899471 KDa

Sequence

String:

MKTILSNQTV DIPENVDITL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELILEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QADE

UniProtKB: Large ribosomal subunit protein uL6

Macromolecule #42: MKI67 FHA domain-interacting nucleolar phosphoprotein

• Macromolecule: Name: MKI67 FHA domain-interacting nucleolar phosphoprotein / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 34.285309 KDa

Sequence

String:

MATFSGPAGP ILSLNPQEDV EFQKEVAQVR KRITQRKKQE QLTPGVVYVR HLPNLLDETQ IFSYFSQFGT VTRFRLSRSK RTGNSKGYA FVEFESEDVA KIVAETMNNY LFGERLLECH FMPPEKVHKE LFKDWNIPFK QPSYPSVKRY NRNRTLTQKL R MEERFKKK ERLLRKKLAK KGIDYDFPSL ILQKTESISK TNRQTSTKGQ VLRKKKKKVS GTLDTPEKTV DSQGPTPVCT PT FLERRKS QVAELNDDDK DDEIVFKQPI SCVKEEIQET QTPTHSRKKR RRSSNQ

UniProtKB: MKI67 FHA domain-interacting nucleolar phosphoprotein

Macromolecule #43: 60S ribosomal protein L7-like 1

• Macromolecule: Name: 60S ribosomal protein L7-like 1 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 29.730354 KDa

Sequence

String:

MISSCTTRKM AEQEQRKIPL VPENLLKKRK AYQALKATQA KQALLAKKEQ KKGKGLRFKR LESFLHDSWR QKRDKVRLRR LEVKPHALE LPDKHSLAFV VRIERIDGVS LLVQRTIARL RLKKIFSGVF VKVTPQNLKM LRIVEPYVTW GFPNLKSVRE L ILKRGQAK VKNKTIPLTD NTVIEEHLGK FGVICLEDLI HEIAFPGKHF QEISWFLCPF HLSVARHATK NRVGFLKEMG TP GYRGERI NQLIRQLN

UniProtKB: Ribosomal protein uL30-like

Macromolecule #44: pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3

• Macromolecule: Name: pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO; EC number: Transferases; Transferring one-carbon groups; Methyltransferases
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 96.726875 KDa

Sequence

String:

MGKKGKVGKS RRDKFYHLAK ETGYRSRSAF KLIQLNRRFQ FLQKARALLD LCAAPGGWLQ VAAKFMPVSS LIVGVDLVPI KPLPNVVTL QQDITTERCR QALRKELKTW KVDVVLNDGA PNVGASWVHD AYSQAHLTLM ALRLACDFLA RGGSFITKVF R SRDYQPLL WIFQQLFRRV QATKPQASRH ESAEIFVVCQ GFLAPDKVDS KFFDPKFAFK EVEVQAKTVT ELVTKKKPKA EG YAEGDLT LYHRTSVTDF LRAANPVDFL SKASEIMVDD EELAQHPATT EDIRVCCQDI RVLGRKELRS LLNWRTKLRR YVA KKLKEQ AKALDISLSS GEEDEGDEED STAGTTKQPS KEEEEEEEEE QLNQTLAEMK AQEVAELKRK KKKLLREQRK QRER VELKM DLPGVSIADE GETGMFSLST IRGHQLLEEV TQGDMSAADT FLSDLPRDDI YVSDVEDDGD DTSLDSDLDP EELAG VRGH QGLRDQKRMR LTEVQDDKEE EEEENPLLVP LEEKAVLQEE QANLWFSKGS FAGIEDDADE ALEISQAQLL FENRRK GRQ QQQKQQLPQT PPSCLKTEIM SPLYQDEAPK GTEASSGTEA ATGLEGEEKD GISDSDSSTS SEEEESWEPL RGKKRSR GP KSDDDGFEIV PIEDPAKHRI LDPEGLALGA VIASSKKAKR DLIDNSFNRY TFNEDEGELP EWFVQEEKQH RIRQLPVG K KEVEHYRKRW REINARPIKK VAEAKARKKR RMLKRLEQTR KKAEAVVNTV DISEREKVAQ LRSLYKKAGL GKEKRHVTY VVAKKGVGRK VRRPAGVRGH FKVVDSRMKK DQRAQQRKEQ KKKHKRK

UniProtKB: pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3

Macromolecule #45: Eukaryotic translation initiation factor 6

• Macromolecule: Name: Eukaryotic translation initiation factor 6 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 26.62001 KDa

Sequence

String:

MAVRASFENN CEIGCFAKLT NTYCLVAIGG SENFYSVFEG ELSDTIPVVH ASIAGCRIIG RMCVGNRHGL LVPNNTTDQE LQHIRNSLP DTVQIRRVEE RLSALGNVTT CNDYVALVHP DLDRETEEIL ADVLKVEVFR QTVADQVLVG SYCVFSNQGG L VHPKTSIE DQDELSSLLQ VPLVAGTVNR GSEVIAAGMV VNDWCAFCGL DTTSTELSVV ESVFKLNEAQ PSTIATSMRD SL IDSLT

UniProtKB: Eukaryotic translation initiation factor 6

Macromolecule #46: Ribosomal L1 domain-containing protein 1

• Macromolecule: Name: Ribosomal L1 domain-containing protein 1 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 55.08975 KDa

Sequence

String:

MEDSASASLS SAAATGTSTS TPAAPTARKQ LDKEQVRKAV DALLTHCKSR KNNYGLLLNE NESLFLMVVL WKIPSKELRV RLTLPHSIR SDSEDICLFT KDEPNSTPEK TEQFYRKLLN KHGIKTVSQI ISLQTLKKEY KSYEAKLRLL SSFDFFLTDA R IRRLLPSL IGRHFYQRKK VPVSVNLLSK NLSREINDCI GGTVLNISKS GSCSAIRIGH VGMQIEHIIE NIVAVTKGLS EK LPEKWES VKLLFVKTEK SAALPIFSSF VSNWDEATKR SLLNKKKKEA RRKRRERNFE KQKERKKKRQ QARKTASVLS KDD VAPESG DTTVKKPESK KEQTPEHGKK KRGRGKAQVK ATNESEDEIP QLVPIGKKTP ANEKVEIQKH ATGKKSPAKS PNPS TPRGK KRKALPASET PKAAESETPG KSPEKKPKIK EEAVKEKSPS LGKKDARQTP KKPEAKFFTT PSKSVRKASH TPKKW PKKP KVPQST

UniProtKB: Ribosomal L1 domain-containing protein 1

Macromolecule #47: Pescadillo homolog

• Macromolecule: Name: Pescadillo homolog / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 68.114727 KDa

Sequence

String:

MGGLEKKKYE RGSATNYITR NKARKKLQLS LADFRRLCIL KGIYPHEPKH KKKVNKGSTA ARTFYLIKDI RFLLHEPIVN KFREYKVFV RKLRKAYGKS EWNTVERLKD NKPNYKLDHI IKERYPTFID ALRDLDDALS MCFLFSTFPR TGKCHVQTIQ L CRRLTVEF MHYIIAARAL RKVFLSIKGI YYQAEVLGQP IVWITPYAFS HDHPTDVDYR VMATFTEFYT TLLGFVNFRL YQ LLNLHYP PKLEGQAQAE AKAGEGTYAL DSESCMEKLA ALSASLARVV VPATEEEAEV DEFPTDGEMS AQEEDRRKEL EAQ EKHKKL FEGLKFFLNR EVPREALAFI IRSFGGEVSW DKSLCIGATY DVTDSRITHQ IVDRPGQQTS VIGRCYVQPQ WVFD SVNAR LLLPVAEYFS GVQLPPHLSP FVTEKEGDYV PPEKLKLLAL QRGEDPGNLN ESEEEEEEDD NNEGDGDEEG ENEEE EEDA EAGSEKEEEA RLAALEEQRM EGKKPRVMAG TLKLEDKQRL AQEEESEAKR LAIMMMKKRE KYLYQKIMFG KRRKIR EAN KLAEKRKAHD EAVRSEKKAK KARPE

UniProtKB: Pescadillo homolog

Macromolecule #48: Probable rRNA-processing protein EBP2

• Macromolecule: Name: Probable rRNA-processing protein EBP2 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 34.925145 KDa

Sequence

String:

MDTPPLSDSE SESDESLVTD RELQDAFSRG LLKPGLNVVL EGPKKAVNDV NGLKQCLAEF KRDLEWVERL DVTLGPVPEI GGSEAPAPQ NKDQKAVDPE DDFQREMSFY RQAQAAVLAV LPRLHQLKVP TKRPTDYFAE MAKSDLQMQK IRQKLQTKQA A MERSEKAK QLRALRKYGK KVQTEVLQKR QQEKAHMMNA IKKYQKGFSD KLDFLEGDQK PLAQRKKAGA KGQQMRKGPS AK RRYKNQK FGFGGKKKGS KWNTRESYDD VSSFRAKTAH GRGLKRPGKK GSNKRPGKRT REKMKNRTH

UniProtKB: Probable rRNA-processing protein EBP2

Macromolecule #49: Ribosome biogenesis protein BRX1 homolog

• Macromolecule: Name: Ribosome biogenesis protein BRX1 homolog / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 41.483516 KDa

Sequence

String:

MAATKRKRRG GFAVQAKKPK RNEIDAEPPA KRHATAEEVE EEERDRIPGP VCKGKWKNKE RILIFSSRGI NFRTRHLMQD LRMLMPHSK ADTKMDRKDK LFVINEVCEM KNCNKCIYFE AKKKQDLYMW LSNSPHGPSA KFLVQNIHTL AELKMTGNCL K GSRPLLSF DPAFDELPHY ALLKELLIQI FSTPRYHPKS QPFVDHVFTF TILDNRIWFR NFQIIEEDAA LVEIGPRFVL NL IKIFQGS FGGPTLYENP HYQSPNMHRR VIRSITAAKY REKQQVKDVQ KLRKKEPKTL LPHDPTADVF VTPAEEKPIE IQW VKPEPK VDLKARKKRI YKRQRKMKQR MDSGKTK

UniProtKB: Ribosome biogenesis protein BRX1 homolog

Macromolecule #50: Ribosome biogenesis protein WDR12

• Macromolecule: Name: Ribosome biogenesis protein WDR12 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 47.768867 KDa

Sequence

String:

MAQLQTRFYT DNKKYAVDDV PFSIPAASEI ADLSNIINKL LKDKNEFHKH VEFDFLIKGQ FLRMPLDKHM EMENISSEEV VEIEYVEKY TAPQPEQCMF HDDWISSIKG AEEWILTGSY DKTSRIWSLE GKSIMTIVGH TDVVKDVAWV KKDSLSCLLL S ASMDQTIL LWEWNVERNK VKALHCCRGH AGSVDSIAVD GSGTKFCSGS WDKMLKIWST VPTDEEDEME ESTNRPRKKQ KT EQLGLTR TPIVTLSGHM EAVSSVLWSD AEEICSASWD HTIRVWDVES GSLKSTLTGN KVFNCISYSP LCKRLASGST DRH IRLWDP RTKDGSLVSL SLTSHTGWVT SVKWSPTHEQ QLISGSLDNI VKLWDTRSCK APLYDLAAHE DKVLSVDWTD TGLL LSGGA DNKLYSYRYS PTTSHVGA

UniProtKB: Ribosome biogenesis protein WDR12

Macromolecule #51: mRNA turnover protein 4 homolog

• Macromolecule: Name: mRNA turnover protein 4 homolog / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 27.602535 KDa

Sequence

String:

MPKSKRDKKV SLTKTAKKGL ELKQNLIEEL RKCVDTYKYL FIFSVANMRN SKLKDIRNAW KHSRMFFGKN KVMMVALGRS PSDEYKDNL HQVSKRLRGE VGLLFTNRTK EEVNEWFTKY TEMDYARAGN KAAFTVSLDP GPLEQFPHSM EPQLRQLGLP T ALKRGVVT LLSDYEVCKE GDVLTPEQAR VLKLFGYEMA EFKVTIKYMW DSQSGRFQQM GDDLPESASE STEESDSEDD D

UniProtKB: mRNA turnover protein 4 homolog

Macromolecule #52: GTP-binding protein 4

• Macromolecule: Name: GTP-binding protein 4 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 74.10782 KDa

Sequence

String:

MAHYNFKKIT VVPSAKDFID LTLSKTQRKT PTVIHKHYQI HRIRHFYMRK VKFTQQNYHD RLSQILTDFP KLDDIHPFYA DLMNILYDK DHYKLALGQI NIAKNLVDNV AKDYVRLMKY GDSLYRCKQL KRAALGRMCT VIKRQKQSLE YLEQVRQHLS R LPTIDPNT RTLLLCGYPN VGKSSFINKV TRADVDVQPY AFTTKSLFVG HMDYKYLRWQ VVDTPGILDH PLEDRNTIEM QA ITALAHL RAAVLYVMDL SEQCGHGLRE QLELFQNIRP LFINKPLIVV ANKCDVKRIA ELSEDDQKIF TDLQSEGFPV IET STLTEE GVIKVKTEAC DRLLAHRVET KMKGNKVNEV LNRLHLAIPT RRDDKERPPF IPEGVVARRK RMETEESRKK RERD LELEM GDDYILDLQK YWDLMNLSEK HDKIPEIWEG HNIADYIDPA IMKKLEELEK EEELRTAAGE YDSVSESEDE EMLEI RQLA KQIREKKKLK ILESKEKNTQ GPRMPRTAKK VQRTVLEKEM RSLGVDMDDK DDAHYAVQAR RSRSITRKRK REDSAP PSS VARSGSCSRT PRDVSGLRDV KMVKKAKTMM KNAQKKMNRL GKKGEADRHV FDMKPKHLLS GKRKAGKKDR R

UniProtKB: GTP-binding protein 4

Macromolecule #53: Ribosome biogenesis protein BOP1

• Macromolecule: Name: Ribosome biogenesis protein BOP1 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 83.901469 KDa

Sequence

String:

MAGSRGAGRT AAPSVRPEKR RSEPELEPEP EPEPPLLCTS PLSHSTGSDS GVSDSEESVF SGLEDSGSDS SEDDDEGDEE GEDGALDDE GHSGIKKTTE EQVQASTPCP RTEMASARIG DEYAED(SEP)(SEP)DE EDIRNTVGNV PLEWYDDFPH VGY DLDGRR IYKPLRTRDE LDQFLDKMDD PDYWRTVQDP MTGRDLRLTD EQVALVRRLQ SGQFGDVGFN PYEPAVDFFS GDVM IHPVT NRPADKRSFI PSLVEKEKVS RMVHAIKMGW IQPRRPRDPT PSFYDLWAQE DPNAVLGRHK MHVPAPKLAL PGHAE SYNP PPEYLLSEEE RLAWEQQEPG ERKLSFLPRK FPSLRAVPAY GRFIQERFER CLDLYLCPRQ RKMRVNVDPE DLIPKL PRP RDLQPFPTCQ ALVYRGHSDL VRCLSVSPGG QWLVSGSDDG SLRLWEVATA RCVRTVPVGG VVKSVAWNPS PAVCLVA AA VEDSVLLLNP ALGDRLVAGS TDQLLSAFVP PEEPPLQPAR WLEASEEERQ VGLRLRICHG KPVTQVTWHG RGDYLAVV L ATQGHTQVLI HQLSRRRSQS PFRRSHGQVQ RVAFHPARPF LLVASQRSVR LYHLLRQELT KKLMPNCKWV SSLAVHPAG DNVICGSYDS KLVWFDLDLS TKPYRMLRHH KKALRAVAFH PRYPLFASGS DDGSVIVCHG MVYNDLLQNP LLVPVKVLKG HVLTRDLGV LDVIFHPTQP WVFSSGADGT VRLFT

UniProtKB: Ribosome biogenesis protein BOP1

Macromolecule #54: Ribosome biogenesis regulatory protein homolog

• Macromolecule: Name: Ribosome biogenesis regulatory protein homolog / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 41.278523 KDa

Sequence

String:

MEGQSVEELL AKAEQDEAEK LQRITVHKEL ELQFDLGNLL ASDRNPPTGL RCAGPTPEAE LQALARDNTQ LLINQLWQLP TERVEEAIV ARLPEPTTRL PREKPLPRPR PLTRWQQFAR LKGIRPKKKT NLVWDEVSGQ WRRRWGYQRA RDDTKEWLIE V PGNADPLE DQFAKRIQAK KERVAKNELN RLRNLARAHK MQLPSAAGLH PTGHQSKEEL GRAMQVAKVS TASVGRFQER LP KEKVPRG SGKKRKFQPL FGDFAAEKKN QLELLRVMNS KKPQLDVTRA TNKQMREEDQ EEAAKRRKMS QKGKRKGGRQ GPG GKRKGG PPSQGGKRKG GLGGKMNSGP PGLGGKRKGG QRPGGKRRK

UniProtKB: Ribosome biogenesis regulatory protein homolog

Macromolecule #55: Nucleolar complex protein 3 homolog

• Macromolecule: Name: Nucleolar complex protein 3 homolog / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 92.709945 KDa

Sequence

String:

MKARRNKKQI PSFRKLIKTS KVKLENKLKN KQFKQQSTLK KYRKEQRKLR QAVKDAVSKK PIPLENPKEK RPGKRIEREE EEEEEALPL DMMDEDDLQL MKDLGQRVSF LTRDLSSSEP VHAKKRKHER IIDKYEKIPR TLQTAPEKEL IHLLPIKDKS G IIPQTREK PVTDSNKDEE DQEEERELEE EIIEDPIQEL TIEEHLIERK KKLQEKKMHI AALASAILSD PENNIKKLKE LR SMLMEQD PDVAVTVRKL VIVSLMELFK DITPSYKIRP LTEAEKSTKT RKETQKLREF EEGLVSQYKF YLENLEQMVK DWK QRKLKK SNVVSLKAYK GLAEVAVKSL CELLVALPHF NFHNNIIVLI VPLMNDMSKL ISEMCCEAVK KLFKQDKLGQ ASLG VIKVI SGFVKGRNYE VRPEMLKTFL CLRIKEVEVK KDTEDINKPK KFMTFKEKRK SLSRMQRKWK KAEEKLEREL REAEA SEST EKKLKLHTET LNIVFVTYFR ILKKAQRSPL LPAVLEGLAK FAHLINVEFF DDLLVVLHTL IESGDLSYQE SLHCVQ TAF HILSGQGDVL NIDPLKFYTH LYKTLFKLHA GATNEGVEIV LQCLDVMLTK RRKQVSQQRA LAFIKRLCTL ALHVLPN SS IGILATTRIL MHTFPKTDLL LDSESQGSGV FLPELDEPEY CNAQNTALWE LHALRRHYHP IVQRFAAHLI AGAPSEGS G ALKPELSRRS ATELFEAYSM AEMTFNPPVE SSNPKIKGKF LQGDSFLNED LNQLIKRYSS EVATESPLDF TKYLKTSLH

UniProtKB: Nucleolar complex protein 3 homolog

Macromolecule #56: Probable ribosome biogenesis protein RLP24

• Macromolecule: Name: Probable ribosome biogenesis protein RLP24 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 19.666258 KDa

Sequence

String:

MRIEKCYFCS GPIYPGHGMM FVRNDCKVFR FCKSKCHKNF KKKRNPRKVR WTKAFRKAAG KELTVDNSFE FEKRRNEPIK YQRELWNKT IDAMKRVEEI KQKRQAKFIM NRLKKNKELQ KVQDIKEVKQ NIHLIRAPLA GKGKQLEEKM VQQLQEDVDM E DAP

UniProtKB: Probable ribosome biogenesis protein RLP24

Macromolecule #57: ATP-dependent RNA helicase DDX18

• Macromolecule: Name: ATP-dependent RNA helicase DDX18 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 75.526789 KDa

Sequence

String:

MSHLPMKLLR KKIEKRNLKL RQRNLKFQGA SNLTLSETQN GDVSEETMGS RKVKKSKQKP MNVGLSETQN GGMSQEAVGN IKVTKSPQK STVLTNGEAA MQSSNSESKK KKKKKRKMVN DAEPDTKKAK TENKGKSEEE SAETTKETEN NVEKPDNDED E SEVPSLPL GLTGAFEDTS FASLCNLVNE NTLKAIKEMG FTNMTEIQHK SIRPLLEGRD LLAAAKTGSG KTLAFLIPAV EL IVKLRFM PRNGTGVLIL SPTRELAMQT FGVLKELMTH HVHTYGLIMG GSNRSAEAQK LGNGINIIVA TPGRLLDHMQ NTP GFMYKN LQCLVIDEAD RILDVGFEEE LKQIIKLLPT RRQTMLFSAT QTRKVEDLAR ISLKKEPLYV GVDDDKANAT VDGL EQGYV VCPSEKRFLL LFTFLKKNRK KKLMVFFSSC MSVKYHYELL NYIDLPVLAI HGKQKQNKRT TTFFQFCNAD SGTLL CTDV AARGLDIPEV DWIVQYDPPD DPKEYIHRVG RTARGLNGRG HALLILRPEE LGFLRYLKQS KVPLSEFDFS WSKISD IQS QLEKLIEKNY FLHKSAQEAY KSYIRAYDSH SLKQIFNVNN LNLPQVALSF GFKVPPFVDL NVNSNEGKQK KRGGGGG FG YQKTKKVEKS KIFKHISKKS SDSRQFSH

UniProtKB: ATP-dependent RNA helicase DDX18

Macromolecule #58: Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase

• Macromolecule: Name: Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase; type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO; EC number: Transferases; Transferring one-carbon groups; Methyltransferases
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 89.441836 KDa

Sequence

String:

MGRKLDPTKE KRGPGRKARK QKGAETELVR FLPAVSDENS KRLSSRARKR AAKRRLGSVE APKTNKSPEA KPLPGKLPKG ISAGAVQTA GKKGPQSLFN APRGKKRPAP GSDEEEEEED SEEDGMVNHG DLWGSEDDAD TVDDYGADSN SEDEEEGEAL L PIERAARK QKAREAAAGI QWSEEETEDE EEEKEVTPES GPPKVEEADG GLQINVDEEP FVLPPAGEME QDAQAPDLQR VH KRIQDIV GILRDFGAQR EEGRSRSEYL NRLKKDLAIY YSYGDFLLGK LMDLFPLSEL VEFLEANEVP RPVTLRTNTL KTR RRDLAQ ALINRGVNLD PLGKWSKTGL VVYDSSVPIG ATPEYLAGHY MLQGASSMLP VMALAPQEHE RILDMCCAPG GKTS YMAQL MKNTGVILAN DANAERLKSV VGNLHRLGVT NTIISHYDGR QFPKVVGGFD RVLLDAPCSG TGVISKDPAV KTNKD EKDI LRCAHLQKEL LLSAIDSVNA TSKTGGYLVY CTCSITVEEN EWVVDYALKK RNVRLVPTGL DFGQEGFTRF RERRFH PSL RSTRRFYPHT HNMDGFFIAK FKKFSNSIPQ SQTGNSETAT PTNVDLPQVI PKSENSSQPA KKAKGAAKTK QQLQKQQ HP KKASFQKLNG ISKGADSELS TVPSVTKTQA SSSFQDSSQP AGKAEGIREP KVTGKLKQRS PKLQSSKKVA FLRQNAPP K GTDTQTPAVL SPSKTQATLK PKDHHQPLGR AKGVEKQQLP EQPFEKAAFQ KQNDTPKGPQ PPTVSPIRSS RPPPAKRKK SQSRGNSQLL LS

UniProtKB: 28S rRNA (cytosine(4447)-C(5))-methyltransferase

Macromolecule #59: Nucleolar protein 16

• Macromolecule: Name: Nucleolar protein 16 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 21.234486 KDa

Sequence

String:

MPKAKGKTRR QKFGYSVNRK RLNRNARRKA APRIECSHIR HAWDHAKSVR QNLAEMGLAV DPNRAVPLRK RKVKAMEVDI EERPKELVR KPYVLNDLEA EASLPEKKGN TLSRDLIDYV RYMVENHGED YKAMARDEKN YYQDTPKQIR SKINVYKRFY P AEWQDFLD SLQKRKMEVE

UniProtKB: Nucleolar protein 16

Macromolecule #4: 5.8S rRNA

• Macromolecule: Name: 5.8S rRNA / type: rna / ID: 4 / Number of copies: 1
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 50.449812 KDa

Sequence

String:

CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGCUGCGAG AAUUAAUGUG AAUUGCAGGA CACAUUGAU CAUCGACACU UCGAACGCAC UUGCGGCCCC GGGUUCCUCC CGGGGCUACG CCUGUCUGAG CGUCGCUU

GENBANK: GENBANK: U13369.1

Macromolecule #5: ITS2 rRNA

• Macromolecule: Name: ITS2 rRNA / type: rna / ID: 5 / Number of copies: 1
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 376.425312 KDa

Sequence

String:

GCCGAUCAAU CGCCCCCGGG GGUGCCUCCG GGCUCCUCGG GGUGCGCGGC UGGGGGUUCC CUCGCAGGGC CCGCCGGGGG CCCUCCGUC CCCCUAAGCG CAGACCCGGC GGCGUCCGCC CUCCUCUUGC CGCCGCGCCC GCCCCUUCCC CCUCCCCCCG C GGGCCCUG CGUGGUCACG CGUCGGGUGG CGGGGGGGAG AGGGGGGCGC GCCCGGCUGA GAGAGACGGG GAGGGCGGCG CC GCCGCCG CCCGCGAAGA CGGAGAGGGA AAGAGAGAGC CGGCUCGGGC CGAGUUCCCG UGGCCGCCGC CUGCGGUCCG GGU UCCUCC CUCGGGGGGC UCCCUCGCGC CGCGCGCGGC UCGGGGUUCG GGGUUCGUCG GCCCCGGCCG GGUGGAAGGU CCCG UGCCC GUCGUCGUCG UCGUCGUCGC GCGUCGUCGG CGGUGGGGGC GUGUUGCGUG CGGUGUGGUG GUGGGGGAGG AGGAA GGCG GGUCCGGAAG GGGAAGGGUG CCGGCGGGGA GAGAGGGUCG GGGGAGCGCG UCCCGGUCGC CGCGGUUCGC CGCCCG CCC CCGGUGGCGG CCCGGCGUCC GGCCGACCGC CGCUCCCGCG CCCCUCCUCC UCCCCGCCGC CCCUCCUCCG AGGCCCC GC CCGUCCUCCU CGCCCUCCCC GCGCGUACGC GCGCCCGCCC GCCCGGCUCG CCUCGCGGCG CGUCGGCCGG GGCCGGGA G CCCGCCCCGC GGCCCGCCCG GCCGCGCCCG UGGCCGCGGC GCCGGGGUUC GCGUGUCCCC GGCGGCGACC CGCGGGACG CCGCGGUGUC GUCCGCCGUC GCGCGCCCGC CUCCGGCUCG CGGCCGCGCC GCGCCGCGCC GGGGCCCCGU CCCGAGCUUC CGCGUCGGG GCGGGGCGGC UCCGCCGCCG CGUCCUCGGA CCCGUCCCCC CGACCUCCGC GGGGGAGACG GGUCGGGGCG U GCGGCGCC CGUCCCGCCC CCGGCCCGUG CCCCUCCCUC CGGUCGUCCC GCUCCGGCGG GGCGGCGCGG GGGUGCCGCC GG CCGCGCG CUCUCUCUCC CGUCGCCUCU CCCCCUCGCC GGGCCCGUCU CCCGACGGAG CGUCGGGCGG GCGGUCGGGC CGG CGCGAU UCCGUCCGUC CGUCCGCCGA GCGGCCCGUC CCCCUCCGAG A

GENBANK: GENBANK: AL592188.60

Macromolecule #6: 28S rRNA

• Macromolecule: Name: 28S rRNA / type: rna / ID: 6 / Number of copies: 1
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 1.6402211249999998 MDa

Sequence

String:

CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAG AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGCGGG GCGCGGGACA UGUGGCGUAC G GAAGACCC GCUCCCCGGC GCCGCUCGUG GGGGGCCCAA GUCCUUCUGA UCGAGGCCCA GCCCGUGGAC GGUGUGAGGC CG GUAGCGG CCCCCGGCGC GCCGGGCCCG GGUCUUCCCG GAGUCGGGUU GCUUGGGAAU GCAGCCCAAA GCGGGUGGUA AAC UCCAUC UAAGGCUAAA UACCGGCACG AGACCGAUAG UCAACAAGUA CCGUAAGGGA AAGUUGAAAA GAACUUUGAA GAGA GAGUU CAAGAGGGCG UGAAACCGUU AAGAGGUAAA CGGGUGGGGU CCGCGCAGUC CGCCCGGAGG AUUCAACCCG GCGGC GGGU CCGGCCGUGU CGGCGGCCCG GCGGAUCUUU CCCGCCCCCC GUUCCUCCCG ACCCCUCCAC CCGCCCUCCC UUCCCC CGC CGCCCCUCCU CCUCCUCCCC GGAGGGGGCG GGCUCCGGCG GGUGCGGGGG UGGGCGGGCG GGGCCGGGGG UGGGGUC GG CGGGGGACCG UCCCCCGACC GGCGACCGGC CGCCGCCGGG CGCAUUUCCA CCGCGGCGGU GCGCCGCGAC CGGCUCCG G GACGGCUGGG AAGGCCCGGC GGGGAAGGUG GCUCGGGGGG CCCCGUCCGU CCGUCCGUCC GUCCUCCUCC UCCCCCGUC UCCGCCCCCC GGCCCCGCGU CCUCCCUCGG GAGGGCGCGC GGGUCGGGGC GGCGGCGGCG GCGGCGGUGG CGGCGGCGGC GGCGGCGGC GGGACCGAAA CCCCCCCCGA GUGUUACAGC CCCCCCGGCA GCAGCACUCG CCGAAUCCCG GGGCCGAGGG A GCGAGACC CGUCGCCGCG CUCUCCCCCC UCCCGGCGCC CACCCCCGCG GGGAAUCCCC CGCGAGGGGG GUCUCCCCCG CG GGGGCGC GCCGGCGUCU CCUCGUGGGG GGGCCGGGCC ACCCCUCCCA CGGCGCGACC GCUCUCCCAC CCCUCCUCCC CGC GCCCCC GCCCCGGCGA CGGGGGGGGU GCCGCGCGCG GGUCGGGGGG CGGGGCGGAC UGUCCCCAGU GCGCCCCGGG CGGG UCGCG CCGUCGGGCC CGGGGGAGGU UCUCUCGGGG CCACGCGCGC GUCCCCCGAA GAGGGGGACG GCGGAGCGAG CGCAC GGGG UCGGCGGCGA CGUCGGCUAC CCACCCGACC CGUCUUGAAA CACGGACCAA GGAGUCUAAC ACGUGCGCGA GUCGGG GGC UCGCACGAAA GCCGCCGUGG CGCAAUGAAG GUGAAGGCCG GCGCGCUCGC CGGCCGAGGU GGGAUCCCGA GGCCUCU CC AGUCCGCCGA GGGCGCACCA CCGGCCCGUC UCGCCCGCCG CGCCGGGGAG GUGGAGCACG AGCGCACGUG UUAGGACC C GAAAGAUGGU GAACUAUGCC UGGGCAGGGC GAAGCCAGAG GAAACUCUGG UGGAGGUCCG UAGCGGUCCU GACGUGCAA AUCGGUCGUC CGACCUGGGU AUAGGGGCGA AAGACUAAUC GAACCAUCUA GUAGCUGGUU CCCUCCGAAG UUUCCCUCAG GAUAGCUGG CGCUCUCGCA GACCCGACGC ACCCCCGCCA CGCAGUUUUA UCCGGUAAAG CGAAUGAUUA GAGGUCUUGG G GCCGAAAC GAUCUCAACC UAUUCUCAAA CUUUAAAUGG GUAAGAAGCC CGGCUCGCUG GCGUGGAGCC GGGCGUGGAA UG CGAGUGC CUAGUGGGCC ACUUUUGGUA AGCAGAACUG GCGCUGCGGG AUGAACCGAA CGCCGGGUUA AGGCGCCCGA UGC CGACGC UCAUCAGACC CCAGAAAAGG UGUUGGUUGA UAUAGACAGC AGGACGGUGG CCAUGGAAGU CGGAAUCCGC UAAG GAGUG UGUAACAACU CACCUGCCGA AUCAACUAGC CCUGAAAAUG GAUGGCGCUG GAGCGUCGGG CCCAUACCCG GCCGU CGCC GGCAGUCGAG AGUGGACGGG AGCGGCGGGG GCGGCGCGCG CGCGCGCGCG UGUGGUGUGC GUCGGAGGGC GGCGGC GGC GGCGGCGGCG GGGGUGUGGG GUCCUUCCCC CGCCCCCCCC CCCACGCCUC CUCCCCUCCU CCCGCCCACG CCCCGCU CC CCGCCCCCGG AGCCCCGCGG ACGCUACGCC GCGACGAGUA GGAGGGCCGC UGCGGUGAGC CUUGAAGCCU AGGGCGCG G GCCCGGGUGG AGCCGCCGCA GGUGCAGAUC UUGGUGGUAG UAGCAAAUAU UCAAACGAGA ACUUUGAAGG CCGAAGUGG AGAAGGGUUC CAUGUGAACA GCAGUUGAAC AUGGGUCAGU CGGUCCUGAG AGAUGGGCGA GCGCCGUUCC GAAGGGACGG GCGAUGGCC UCCGUUGCCC GCCGCCGAUC GAAAGGGAGU CGGGUUCAGA UCCCCGAAUC CGGAGUGGCG GAGAUGGGCG C CGCGAGGC GUCCAGUGCG GUAACGCGAC CGAUCCCGGA GAAGCCGGCG GGAGCCCCGG GGAGAGUUCU CUUUUCUUUG UG AAGGGCA GGGCGCCCUG GAAUGGGUUC GCCCCGAGAG AGGGGCCCGU GCCUUGGAAA GCGUCGCGGU UCCGGCGGCG UCC GGUGAG CUCUCGCUGG CCCUUGAAAA UCCGGGGGAG AGGGUGUAAA UCUCGCGCCG GGCCGUACCC AUAUCCGCAG CAGG UCUCC AAGGUGAACA GCCUCUGGCA UGUUGGAACA AUGUAGGUAA GGGAAGUCGG CAAGCCGGAU CCGUAACUUC GGGAU AAGG AUUGGCUCUA AGGGCUGGGU CGGUCGGGCU GGGGCGCGAA GCGGGGCUGG GCGCGCGCCG CGGCUGGACG AGGCGC CGC CGCCCCCCCC ACGCCCGGGG CACCCCCCUC GCGGCCCUCC CCCGCCCCAC CCCGCGCGCG CCGCUCGCUC CCUCCCC GC CCCGCGCCCU CUCUCUCUCU CUCUCCCCCG CUCCCCGUCC UCCCCCCUCC CCGGGGGAGC GCCGCGUGGG GGCGGCGG C GGGGGGAGAA GGGUCGGGGC GGCAGGGGCC GGCGGCGGCC CGCCGCGGGG CCCCGGCGGC GGGGGCACGG UCCCCCGCG AGGGGGGCCC GGGCACCCGG GGGGCCGGCG GCGGCGGCGA CUCUGGACGC GAGCCGGGCC CUUCCCGUGG AUCGCCCCAG CUGCGGCGG GCGUCGCGGC CGCCCCCGGG GAGCCCGGCG GGCGCCGGCG CGCCCCCCCC CCCACCCCAC GUCUCGUCGC G CGCGCGUC CGCUGGGGGC GGGGAGCGGU CGGGCGGCGG CGGUCGGCGG GCGGCGGGGC GGGGCGGUUC GUCCCCCCGC CC UACCCCC CCGGCCCCGU CCGCCCCCCG UUCCCCCCUC CUCCUCGGCG CGCGGCGGCG GCGGCGGCAG GCGGCGGAGG GGC CGCGGG CCGGUCCCCC CCGCCGGGUC CGCCCCCGGG GCCGCGGUUC CGCGCGGCGC CUCGCCUCGG CCGGCGCCUA GCAG CCGAC UUAGAACUGG UGCGGACCAG GGGAAUCCGA CUGUUUAAUU AAAACAAAGC AUCGCGAAGG CCCGCGGCGG GUGUU GACG CGAUGUGAUU UCUGCCCAGU GCUCUGAAUG UCAAAGUGAA GAAAUUCAAU GAAGCGCGGG UAAACGGCGG GAGUAA CUA UGACUCUCUU AAGGUAGCCA AAUGCCUCGU CAUCUAAUUA GUGACGCGCA UGAAUGGAUG AACGAGAUUC CCACUGU CC CUACCUACUA UCCAGCGAAA CCACAGCCAA GGGAACGGGC UUGGCGGAAU CAGCGGGGAA AGAAGACCCU GUUGAGCU U GACUCUAGUC UGGCACGGUG AAGAGACAUG AGAGGUGUAG AAUAAGUGGG AGGCCCCCGG CGCCCCCCCG GUGUCCCCG CGAGGGGCCC GGGGCGGGGU CCGCCGGCCC UGCGGGCCGC CGGUGAAAUA CCACUACUCU GAUCGUUUUU UCACUGACCC GGUGAGGCG GGGGGGCGAG CCCCGAGGGG CUCUCGCUUC UGGCGCCAAG CGCCCGGCCG CGCGCCGGCC GGGCGCGACC C GCUCCGGG GACAGUGCCA GGUGGGGAGU UUGACUGGGG CGGUACACCU GUCAAACGGU AACGCAGGUG UCCUAAGGCG AG CUCAGGG AGGACAGAAA CCUCCCGUGG AGCAGAAGGG CAAAAGCUCG CUUGAUCUUG AUUUUCAGUA CGAAUACAGA CCG UGAAAG CGGGGCCUCA CGAUCCUUCU GACCUUUUGG GUUUUAAGCA GGAGGUGUCA GAAAAGUUAC CACAGGGAUA ACUG GCUUG UGGCGGCCAA GCGUUCAUAG CGACGUCGCU UUUUGAUCCU UCGAUGUCGG CUCUUCCUAU CAUUGUGAAG CAGAA UUCA CCAAGCGUUG GAUUGUUCAC CCACUAAUAG GGAACGUGAG CUGGGUUUAG ACCGUCGUGA GACAGGUUAG UUUUAC CCU ACUGAUGAUG UGUUGUUGCC AUGGUAAUCC UGCUCAGUAC GAGAGGAACC GCAGGUUCAG ACAUUUGGUG UAUGUGC UU GGCUGAGGAG CCAAUGGGGC GAAGCUACCA UCUGUGGGAU UAUGACUGAA CGCCUCUAAG UCAGAAUCCC GCCCAGGC G GAACGAUACG GCAGCGCCGC GGAGCCUCGG UUGGCCUCGG AUAGCCGGUC CCCCGCCUGU CCCCGCCGGC GGGCCGCCC CCCCCCUCCA CGCGCCCCGC GCGCGCGGGA GGGCGCGUGC CCCGCCGCGC GCCGGGACCG GGGUCCGGUG CGGAGUGCCC UUCGUCCUG GGAAACGGGG CGCGGCCGGA GAGGCGGCCG CCCCCUCGCC CGUCACGCAC CGCACGUUCG UGGGGAACCU G GCGCUAAA CCAUUCGUAG ACGACCUGCU UCUGGGUCGG GGUUUCGUAC GUAGCAGAGC AGCUCCCUCG CUGCGAUCUA UU GAAAGUC AGCCCUCGAC ACAAGGGUUU GUC

GENBANK: GENBANK: AL592188.60

Macromolecule #60: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 60 / Number of copies: 70 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #61: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 61 / Number of copies: 3 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #62: ADENOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 62 / Number of copies: 1 / Formula: ADP
• Molecular weight: Theoretical: 427.201 Da

Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Macromolecule #63: GUANOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 63 / Number of copies: 1 / Formula: GDP
• Molecular weight: Theoretical: 443.201 Da

Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Macromolecule #64: POTASSIUM ION

• Macromolecule: Name: POTASSIUM ION / type: ligand / ID: 64 / Number of copies: 1 / Formula: K
• Molecular weight: Theoretical: 39.098 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.6
• Grid: Model: Quantifoil R3.5/1 / Material: GOLD / Mesh: 400 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec.
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 283 K / Instrument: FEI VITROBOT MARK IV; Details: Four applications with manual blotting before last blotting with the vitrobot..

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Specialist optics: Energy filter - Slit width: 20 eV
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 4 / Number real images: 172699 / Average exposure time: 2.0 sec. / Average electron dose: 60.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 64000
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 15679142
• Startup model: Type of model: INSILICO MODEL
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.67 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 3.3.2) / Number images used: 76907
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 4.0)
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.3.2)
• Final 3D classification: Software: (Name: RELION (ver. 4.0), cryoSPARC (ver. 3.3.2))

Atomic model buiding 1

• Refinement: Space: REAL

Output model

PDB-8fky:
Human nucleolar pre-60S ribosomal subunit (State F)