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- EMDB-29260: Human nucleolar pre-60S ribosomal subunit (State E) - Composite map -
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Open data
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Basic information
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Title | Human nucleolar pre-60S ribosomal subunit (State E) - Composite map | |||||||||
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![]() | Pre-60S ribosomal subunit / Assembly intermediate / Ribosome / Nucleoprotein complex | |||||||||
Function / homology | ![]() RNA 2'-O-methyltransferase activity / Noc1p-Noc2p complex / Noc2p-Noc3p complex / TFAP2A acts as a transcriptional repressor during retinoic acid induced cell differentiation / positive regulation of protein localization to chromosome, telomeric region / RNA metabolic process / basal RNA polymerase II transcription machinery binding / negative regulation of collagen binding / dendrite extension / rRNA (uridine-2'-O-)-methyltransferase activity ...RNA 2'-O-methyltransferase activity / Noc1p-Noc2p complex / Noc2p-Noc3p complex / TFAP2A acts as a transcriptional repressor during retinoic acid induced cell differentiation / positive regulation of protein localization to chromosome, telomeric region / RNA metabolic process / basal RNA polymerase II transcription machinery binding / negative regulation of collagen binding / dendrite extension / rRNA (uridine-2'-O-)-methyltransferase activity / rRNA (guanine) methyltransferase activity / rRNA (cytosine-C5-)-methyltransferase activity / tRNA (cytidine-5-)-methyltransferase activity / preribosome binding / regulation of cellular senescence / lamin filament / regulation of fatty acid biosynthetic process / RNA methylation / regulation of megakaryocyte differentiation / positive regulation of protein sumoylation / miRNA-mediated post-transcriptional gene silencing / stem cell division / miRNA-mediated gene silencing by inhibition of translation / PeBoW complex / negative regulation of formation of translation preinitiation complex / positive regulation of telomere maintenance / ribosomal protein import into nucleus / tRNA methylation / blastocyst formation / rRNA base methylation / TORC2 complex binding / protein localization to nucleolus / GAIT complex / negative regulation of B cell apoptotic process / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA methylation / rRNA modification in the nucleus and cytosol / A band / regulation of reactive oxygen species metabolic process / regulation of G1 to G0 transition / regulation of glycolytic process / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / regulation of translation involved in cellular response to UV / protein-DNA complex disassembly / positive regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / nuclear-transcribed mRNA catabolic process / negative regulation of ubiquitin protein ligase activity / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / preribosome, small subunit precursor / mitotic metaphase chromosome alignment / G1 to G0 transition / stem cell population maintenance / regulation of cyclin-dependent protein serine/threonine kinase activity / homeostatic process / rRNA metabolic process / positive regulation of dendritic spine development / negative regulation of cell-cell adhesion / lung morphogenesis / negative regulation of DNA replication / maturation of 5.8S rRNA / fat cell differentiation / Protein hydroxylation / macrophage chemotaxis / Peptide chain elongation / ribosomal large subunit binding / Selenocysteine synthesis / positive regulation of signal transduction by p53 class mediator / ubiquitin ligase inhibitor activity / Formation of a pool of free 40S subunits / Eukaryotic Translation Termination / rRNA transcription / blastocyst development / Response of EIF2AK4 (GCN2) to amino acid deficiency / SRP-dependent cotranslational protein targeting to membrane / preribosome, large subunit precursor / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Viral mRNA Translation / L13a-mediated translational silencing of Ceruloplasmin expression / GTP hydrolysis and joining of the 60S ribosomal subunit / DNA replication initiation / Major pathway of rRNA processing in the nucleolus and cytosol / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / estrogen receptor signaling pathway / cellular response to interleukin-4 / RNA processing / hematopoietic progenitor cell differentiation / cellular response to actinomycin D / cytosolic ribosome / ribonucleoprotein complex binding / ribosomal subunit export from nucleus / rough endoplasmic reticulum / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / negative regulation of intrinsic apoptotic signaling pathway / nucleosome binding / MDM2/MDM4 family protein binding / negative regulation of protein ubiquitination / transcription initiation-coupled chromatin remodeling / DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest / translation initiation factor activity / negative regulation of ubiquitin-dependent protein catabolic process Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.59 Å | |||||||||
![]() | Vanden Broeck A / Klinge S | |||||||||
Funding support | European Union, ![]()
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![]() | ![]() Title: Principles of human pre-60 biogenesis. Authors: Arnaud Vanden Broeck / Sebastian Klinge / ![]() Abstract: During the early stages of human large ribosomal subunit (60) biogenesis, an ensemble of assembly factors establishes and fine-tunes the essential RNA functional centers of pre-60 particles by an ...During the early stages of human large ribosomal subunit (60) biogenesis, an ensemble of assembly factors establishes and fine-tunes the essential RNA functional centers of pre-60 particles by an unknown mechanism. Here, we report a series of cryo-electron microscopy structures of human nucleolar and nuclear pre-60 assembly intermediates at resolutions of 2.5 to 3.2 angstroms. These structures show how protein interaction hubs tether assembly factor complexes to nucleolar particles and how guanosine triphosphatases and adenosine triphosphatase couple irreversible nucleotide hydrolysis steps to the installation of functional centers. Nuclear stages highlight how a conserved RNA-processing complex, the rixosome, couples large-scale RNA conformational changes with pre-ribosomal RNA processing by the RNA degradation machinery. Our ensemble of human pre-60 particles provides a rich foundation with which to elucidate the molecular principles of ribosome formation. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 58.8 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 87.6 KB 87.6 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 15.8 KB | Display | ![]() |
Images | ![]() | 148.2 KB | ||
Masks | ![]() | 421.9 MB | ![]() | |
Others | ![]() ![]() | 391.1 MB 391.1 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 25 KB | Display | |
Data in CIF | ![]() | 32.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8fkxMC ![]() 8fkpC ![]() 8fkqC ![]() 8fkrC ![]() 8fksC ![]() 8fktC ![]() 8fkuC ![]() 8fkvC ![]() 8fkwC ![]() 8fkyC ![]() 8fkzC ![]() 8fl0C ![]() 8fl2C ![]() 8fl3C ![]() 8fl4C ![]() 8fl6C ![]() 8fl7C ![]() 8fl9C ![]() 8flaC ![]() 8flbC ![]() 8flcC ![]() 8fldC ![]() 8fleC ![]() 8flfC C: citing same article ( M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 1.072 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Half map: #2
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Projections & Slices |
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Density Histograms |
-Half map: #1
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Projections & Slices |
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Density Histograms |
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Sample components
+Entire : Human nucleolar pre-60S ribosomal subunit (State E)
+Supramolecule #1: Human nucleolar pre-60S ribosomal subunit (State E)
+Macromolecule #1: 60S ribosomal protein L12
+Macromolecule #2: 60S ribosomal protein L10a
+Macromolecule #6: 60S ribosomal protein L13
+Macromolecule #7: 60S ribosomal protein L13a
+Macromolecule #8: 60S ribosomal protein L14
+Macromolecule #9: 60S ribosomal protein L15
+Macromolecule #10: 60S ribosomal protein L17
+Macromolecule #11: 60S ribosomal protein L18
+Macromolecule #12: 60S ribosomal protein L18a
+Macromolecule #13: 60S ribosomal protein L21
+Macromolecule #14: 60S ribosomal protein L23
+Macromolecule #15: 60S ribosomal protein L23a
+Macromolecule #16: 60S ribosomal protein L26
+Macromolecule #17: 60S ribosomal protein L27a
+Macromolecule #18: 60S ribosomal protein L28
+Macromolecule #19: 60S ribosomal protein L3
+Macromolecule #20: 60S ribosomal protein L31
+Macromolecule #21: 60S ribosomal protein L32
+Macromolecule #22: 60S ribosomal protein L35
+Macromolecule #23: 60S ribosomal protein L35a
+Macromolecule #24: 60S ribosomal protein L36
+Macromolecule #25: 60S ribosomal protein L37
+Macromolecule #26: Nucleolar complex protein 2 homolog
+Macromolecule #27: Guanine nucleotide-binding protein-like 3
+Macromolecule #28: Ribosome biogenesis protein NSA2 homolog
+Macromolecule #29: 60S ribosome subunit biogenesis protein NIP7 homolog
+Macromolecule #30: ATP-dependent RNA helicase DDX54
+Macromolecule #31: Protein LLP homolog
+Macromolecule #32: 60S ribosomal protein L4
+Macromolecule #33: 60S ribosomal protein L6
+Macromolecule #34: 60S ribosomal protein L7
+Macromolecule #35: 60S ribosomal protein L7a
+Macromolecule #36: 60S ribosomal protein L9
+Macromolecule #37: MKI67 FHA domain-interacting nucleolar phosphoprotein
+Macromolecule #38: 60S ribosomal protein L7-like 1
+Macromolecule #39: pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3
+Macromolecule #40: Eukaryotic translation initiation factor 6
+Macromolecule #41: Ribosomal L1 domain-containing protein 1
+Macromolecule #42: Pescadillo homolog
+Macromolecule #43: Probable rRNA-processing protein EBP2
+Macromolecule #44: Ribosome biogenesis protein BRX1 homolog
+Macromolecule #45: mRNA turnover protein 4 homolog
+Macromolecule #46: GTP-binding protein 4
+Macromolecule #47: Ribosome biogenesis protein BOP1
+Macromolecule #48: Ribosome biogenesis regulatory protein homolog
+Macromolecule #49: Nucleolar complex protein 3 homolog
+Macromolecule #50: Probable ribosome biogenesis protein RLP24
+Macromolecule #51: ATP-dependent RNA helicase DDX18
+Macromolecule #52: Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase
+Macromolecule #53: Nucleolar protein 16
+Macromolecule #3: 5.8S rRNA
+Macromolecule #4: ITS2 rRNA
+Macromolecule #5: 28S rRNA
+Macromolecule #54: MAGNESIUM ION
+Macromolecule #55: ZINC ION
+Macromolecule #56: ADENOSINE-5'-DIPHOSPHATE
+Macromolecule #57: GUANOSINE-5'-DIPHOSPHATE
+Macromolecule #58: POTASSIUM ION
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.6 |
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Grid | Model: Quantifoil R3.5/1 / Material: GOLD / Mesh: 400 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec. |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 283 K / Instrument: FEI VITROBOT MARK IV Details: Four applications with manual blotting before last blotting with the vitrobot.. |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Specialist optics | Energy filter - Slit width: 20 eV |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 4 / Number real images: 172699 / Average exposure time: 2.0 sec. / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 64000 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
-Atomic model buiding 1
Refinement | Space: REAL |
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Output model | ![]() PDB-8fkx: |