タイトル | Structural basis of transcription recognition of a hydrophobic unnatural base pair by T7 RNA polymerase. |
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ジャーナル・号・ページ | Nat Commun, Vol. 14, Page 195-195, Year 2023 |
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掲載日 | 2022年6月24日 (構造データの登録日) |
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著者 | Oh, J. / Kimoto, M. / Xu, H. / Chong, J. / Hirao, I. / Wang, D. |
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リンク | Nat Commun / PubMed:36635281 |
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手法 | X線回折 |
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解像度 | 2.65 - 3 Å |
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構造データ | PDB-8dh0: T7 RNA polymerase elongation complex with unnatural base dDs 手法: X-RAY DIFFRACTION / 解像度: 2.9 Å PDB-8dh1: T7 RNA polymerase elongation complex with unnatural base dDs-PaTP pair 手法: X-RAY DIFFRACTION / 解像度: 2.65 Å PDB-8dh2: T7 RNA polymerase elongation complex with unnatural base dDs-ATP mismatch 手法: X-RAY DIFFRACTION / 解像度: 2.9 Å PDB-8dh3: T7 RNA polymerase elongation complex with unnatural base dPa 手法: X-RAY DIFFRACTION / 解像度: 3 Å PDB-8dh4: T7 RNA polymerase elongation complex with unnatural base dPa-DsTP pair 手法: X-RAY DIFFRACTION / 解像度: 2.8 Å PDB-8dh5: T7 RNA polymerase elongation complex with unnatural base dPa-ATP mismatch 手法: X-RAY DIFFRACTION / 解像度: 2.85 Å |
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化合物 | ChemComp-S8L: 1-{5-O-[(S)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]oxy}phosphoryl]-beta-D-ribofuranosyl}-1H-pyrrole-2-carbaldehyde
ChemComp-S96: (7P)-3-{5-O-[(R)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}phosphoryl]-beta-D-ribofuranosyl}-7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridine
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由来 | ![](img/tx_virus.gif) escherichia phage t7 (ファージ)- synthetic construct (人工物)
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キーワード | Transferase/DNA/RNA / T7 RNA polymerase / elongation complex / unnatural base / Ds-Pa / synthetic DNA / transcription / Transferase-DNA-RNA complex |
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