EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Sarecycline inhibits protein translation in Cutibacterium acnes 70S ribosome using a two-site mechanism.
ジャーナル・号・ページ	Nucleic Acids Res, Vol. 51, Issue 6, Page 2915-2930, Year 2023
掲載日	2023年3月2日
著者	Ivan B Lomakin / Swapnil C Devarkar / Shivali Patel / Ayman Grada / Christopher G Bunick /
PubMed 要旨	Acne vulgaris is a chronic disfiguring skin disease affecting ∼1 billion people worldwide, often having persistent negative effects on physical and mental health. The Gram-positive anaerobe, ...Acne vulgaris is a chronic disfiguring skin disease affecting ∼1 billion people worldwide, often having persistent negative effects on physical and mental health. The Gram-positive anaerobe, Cutibacterium acnes is implicated in acne pathogenesis and is, therefore, a main target for antibiotic-based acne therapy. We determined a 2.8-Å resolution structure of the 70S ribosome of Cutibacterium acnes by cryogenic electron microscopy and discovered that sarecycline, a narrow-spectrum antibiotic against Cutibacterium acnes, may inhibit two active sites of this bacterium's ribosome in contrast to the one site detected previously on the model ribosome of Thermus thermophilus. Apart from the canonical binding site at the mRNA decoding center, the second binding site for sarecycline exists at the nascent peptide exit tunnel, reminiscent of the macrolides class of antibiotics. The structure also revealed Cutibacterium acnes-specific features of the ribosomal RNA and proteins. Unlike the ribosome of the Gram-negative bacterium Escherichia coli, Cutibacterium acnes ribosome has two additional proteins, bS22 and bL37, which are also present in the ribosomes of Mycobacterium smegmatis and Mycobacterium tuberculosis. We show that bS22 and bL37 have antimicrobial properties and may be involved in maintaining the healthy homeostasis of the human skin microbiome.
リンク	Nucleic Acids Res / PubMed:36864821 / PubMed Central
手法	EM (単粒子)
解像度	2.66 - 2.95 Å
構造データ	26959 8crx EMDB-26959, PDB-8crx: Cutibacterium acnes 70S ribosome with mRNA, P-site tRNA and Sarecycline bound 手法: EM (単粒子) / 解像度: 2.78 Å 27009 8cvm EMDB-27009, PDB-8cvm: Cutibacterium acnes 50S ribosomal subunit with P-site tRNA and Sarecycline bound in the local refined map 手法: EM (単粒子) / 解像度: 2.66 Å 27011 8cvo EMDB-27011, PDB-8cvo: Cutibacterium acnes 30S ribosomal subunit with Sarecycline bound, head domain only in the local refined map 手法: EM (単粒子) / 解像度: 2.95 Å 27028 8cwo EMDB-27028, PDB-8cwo: Cutibacterium acnes 30S ribosomal subunit with Sarecycline bound, body domain only in the local refined map 手法: EM (単粒子) / 解像度: 2.84 Å
化合物	ChemComp-V7A: Sarecycline / 薬剤, 抗生剤YM / Sarecycline ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-HOH*: WATER / 水
由来	cutibacterium acnes (バクテリア) escherichia coli (大腸菌)
キーワード	ANTIBIOTIC (抗生物質) / ribosome (リボソーム) / sarecycline / acne (尋常性痤瘡)