EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	CryoEM structural exploration of catalytically active enzyme pyruvate carboxylase.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 6185, Year 2022
掲載日	2022年10月19日
著者	Jorge Pedro López-Alonso / Melisa Lázaro / David Gil-Cartón / Philip H Choi / Alexandra Dodu / Liang Tong / Mikel Valle /
PubMed 要旨	Pyruvate carboxylase (PC) is a tetrameric enzyme that contains two active sites per subunit that catalyze two consecutive reactions. A mobile domain with an attached prosthetic biotin links both ...Pyruvate carboxylase (PC) is a tetrameric enzyme that contains two active sites per subunit that catalyze two consecutive reactions. A mobile domain with an attached prosthetic biotin links both reactions, an initial biotin carboxylation and the subsequent carboxyl transfer to pyruvate substrate to produce oxaloacetate. Reaction sites are at long distance, and there are several co-factors that play as allosteric regulators. Here, using cryoEM we explore the structure of active PC tetramers focusing on active sites and on the conformational space of the oligomers. The results capture the mobile domain at both active sites and expose catalytic steps of both reactions at high resolution, allowing the identification of substrates and products. The analysis of catalytically active PC tetramers reveals the role of certain motions during enzyme functioning, and the structural changes in the presence of additional cofactors expose the mechanism for allosteric regulation.
リンク	Nat Commun / PubMed:36261450 / PubMed Central
手法	EM (単粒子)
解像度	2.12 - 3.29 Å
構造データ	15028 7zyy EMDB-15028, PDB-7zyy: Cryo-EM structure of Lactococcus lactis pyruvate carboxylase with acetyl-CoA 手法: EM (単粒子) / 解像度: 2.12 Å 15029 7zyz EMDB-15029, PDB-7zyz: Cryo-EM structure of "CT oxa" conformation of Lactococcus lactis pyruvate carboxylase with acetyl-CoA 手法: EM (単粒子) / 解像度: 2.47 Å 15030 7zz0 EMDB-15030, PDB-7zz0: Cryo-EM structure of "CT empty" conformation of Lactococcus lactis pyruvate carboxylase with acetyl-CoA 手法: EM (単粒子) / 解像度: 2.26 Å 15031 7zz1 EMDB-15031, PDB-7zz1: Cryo-EM structure of "CT react" conformation of Lactococcus lactis pyruvate carboxylase with acetyl-CoA 手法: EM (単粒子) / 解像度: 2.27 Å 15032 7zz2 EMDB-15032, PDB-7zz2: Cryo-EM structure of "CT pyr" conformation of Lactococcus lactis pyruvate carboxylase with acetyl-CoA 手法: EM (単粒子) / 解像度: 2.48 Å 15033 7zz3 EMDB-15033, PDB-7zz3: Cryo-EM structure of "BC react" conformation of Lactococcus lactis pyruvate carboxylase with acetyl-CoA 手法: EM (単粒子) / 解像度: 2.41 Å 15034 7zz4 EMDB-15034, PDB-7zz4: Cryo-EM structure of "BC closed" conformation of Lactococcus lactis pyruvate carboxylase with acetyl-CoA 手法: EM (単粒子) / 解像度: 2.63 Å 15035 7zz5 EMDB-15035, PDB-7zz5: Cryo-EM structure of "BC open" conformation of Lactococcus lactis pyruvate carboxylase with acetyl-CoA 手法: EM (単粒子) / 解像度: 2.43 Å 15036 7zz6 EMDB-15036, PDB-7zz6: Cryo-EM structure of "CT-CT dimer" of Lactococcus lactis pyruvate carboxylase with acetyl-CoA 手法: EM (単粒子) / 解像度: 2.15 Å 15037 7zz8 EMDB-15037, PDB-7zz8: Cryo-EM structure of Lactococcus lactis pyruvate carboxylase with acetyl-CoA and cyclic di-AMP 手法: EM (単粒子) / 解像度: 3.29 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-MN: Unknown entry ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-ACO: ACETYL COENZYME A / アデノシン3′-りん酸5′-[二りん酸P²-[(R)-3-ヒドロキシ-2,2-ジメチル-3-[[(以下略) / アセチルCoA ChemComp-PYR: PYRUVIC ACID / ピルビン酸 / ピルビン酸 ChemComp-BCT: BICARBONATE ION / 炭酸水素アニオン / pH緩衝剤YM / 炭酸水素塩 ChemComp-HOH: WATER / 水 ChemComp-OAA: OXALOACETATE ION / オキサロ酢酸 ChemComp-BTN: BIOTIN / ビオチン / ビオチン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-2BA: (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8 / c-di-AMP
由来	lactococcus lactis (乳酸菌)
キーワード	LIGASE (リガーゼ) / Tetramer (四量体) / carboxylase (カルボキシル化) / biotin (ビオチン) / inhibitor (酵素阻害剤)