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-Structure paper
タイトル | Structure, dynamics and assembly of the ankyrin complex on human red blood cell membrane. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 29, Issue 7, Page 698-705, Year 2022 |
掲載日 | 2022年6月2日 |
著者 | Xian Xia / Shiheng Liu / Z Hong Zhou / |
PubMed 要旨 | The cytoskeleton of a red blood cell (RBC) is anchored to the cell membrane by the ankyrin complex. This complex is assembled during RBC genesis and comprises primarily band 3, protein 4.2 and ...The cytoskeleton of a red blood cell (RBC) is anchored to the cell membrane by the ankyrin complex. This complex is assembled during RBC genesis and comprises primarily band 3, protein 4.2 and ankyrin, whose mutations contribute to numerous human inherited diseases. High-resolution structures of the ankyrin complex have been long sought-after to understand its assembly and disease-causing mutations. Here, we analyzed native complexes on the human RBC membrane by stepwise fractionation. Cryo-electron microscopy structures of nine band-3-associated complexes reveal that protein 4.2 stabilizes the cytoplasmic domain of band 3 dimer. In turn, the superhelix-shaped ankyrin binds to this protein 4.2 via ankyrin repeats (ARs) 6-13 and to another band 3 dimer via ARs 17-20, bridging two band 3 dimers in the ankyrin complex. Integration of these structures with both prior data and our biochemical data supports a model of ankyrin complex assembly during erythropoiesis and identifies interactions essential for the mechanical stability of RBC. |
リンク | Nat Struct Mol Biol / PubMed:35655099 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.1 - 8.5 Å |
構造データ | EMDB-26142, PDB-7tvz: EMDB-26143: Membrane domain of band3-protein 4.2 complex in diagonal conformation EMDB-26144: Cytoplasmic domain of band 3-protein 4.2 complex in diagonal conformation EMDB-26145: Cryo-EM structure of human band 3-protein 4.2 complex in a loosely-bound conformation EMDB-26146, PDB-7tw0: EMDB-26147, PDB-7tw1: EMDB-26148, PDB-7tw2: EMDB-26149, PDB-7tw3: EMDB-26150: Cytoplasmic domain of ankyrin complex (B2P1A1) EMDB-26151, PDB-7tw5: EMDB-26152: Cytoplasmic domain of ankyrin complex (B2P1A2) EMDB-26153, PDB-7tw6: EMDB-26154: Cryo-EM structure of human ankyrin complex (B2P1A1)2 |
化合物 | ChemComp-NAG: ChemComp-LMT: ChemComp-CLR: ChemComp-HOH: |
由来 |
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キーワード | MEMBRANE PROTEIN / Red blood cell / Ankyrin complex / band 3 / protein 4.2 |