EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for tunable control of actin dynamics by myosin-15 in mechanosensory stereocilia.
ジャーナル・号・ページ	Sci Adv, Vol. 8, Issue 29, Page eabl4733, Year 2022
掲載日	2022年7月22日
著者	Rui Gong / Fangfang Jiang / Zane G Moreland / Matthew J Reynolds / Santiago Espinosa de Los Reyes / Pinar Gurel / Arik Shams / James B Heidings / Michael R Bowl / Jonathan E Bird / Gregory M Alushin /
PubMed 要旨	The motor protein myosin-15 is necessary for the development and maintenance of mechanosensory stereocilia, and mutations in myosin-15 cause hereditary deafness. In addition to transporting actin ...The motor protein myosin-15 is necessary for the development and maintenance of mechanosensory stereocilia, and mutations in myosin-15 cause hereditary deafness. In addition to transporting actin regulatory machinery to stereocilia tips, myosin-15 directly nucleates actin filament ("F-actin") assembly, which is disrupted by a progressive hearing loss mutation (p.D1647G, ""). Here, we present cryo-electron microscopy structures of myosin-15 bound to F-actin, providing a framework for interpreting the impacts of deafness mutations on motor activity and actin nucleation. Rigor myosin-15 evokes conformational changes in F-actin yet maintains flexibility in actin's D-loop, which mediates inter-subunit contacts, while the mutant locks the D-loop in a single conformation. Adenosine diphosphate-bound myosin-15 also locks the D-loop, which correspondingly blunts actin-polymerization stimulation. We propose myosin-15 enhances polymerization by bridging actin protomers, regulating nucleation efficiency by modulating actin's structural plasticity in a myosin nucleotide state-dependent manner. This tunable regulation of actin polymerization could be harnessed to precisely control stereocilium height.
リンク	Sci Adv / PubMed:35857845 / PubMed Central
手法	EM (らせん対称) / EM (単粒子)
解像度	2.62 - 4.18 Å
構造データ	24321 7r8v EMDB-24321, PDB-7r8v: Cryo-EM structure of the ADP state actin filament 手法: EM (らせん対称) / 解像度: 2.82 Å 24322 7r91 EMDB-24322, PDB-7r91: cryo-EM structure of the rigor state wild type myosin-15-F-actin complex 手法: EM (らせん対称) / 解像度: 2.83 Å 24399 7rb8 EMDB-24399, PDB-7rb8: cryo-EM structure of the ADP state wild type myosin-15-F-actin complex 手法: EM (らせん対称) / 解像度: 3.63 Å 24400 7rb9 EMDB-24400, PDB-7rb9: Cryo-EM structure of the rigor state Jordan myosin-15-F-actin complex 手法: EM (らせん対称) / 解像度: 3.76 Å 26459 7udt EMDB-26459, PDB-7udt: cryo-EM structure of the rigor state wild type myosin-15-F-actin complex (symmetry expansion and re-centering) 手法: EM (単粒子) / 解像度: 3.17 Å 26460 7udu EMDB-26460, PDB-7udu: cryo-EM structure of the ADP state wild type myosin-15-F-actin complex (symmetry expansion and re-centering) 手法: EM (単粒子) / 解像度: 4.15 Å EMDB-26461: cryo-EM structure of the ADP state wild type myosin-15-F-actin complex (symmetry expansion) 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-26462: cryo-EM structure of the rigor state wild type myosin-15-F-actin complex (symmetry expansion) 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-26463: cryo-EM structure of the ADP state actin filament (symmetry expansion) 手法: EM (単粒子) / 解像度: 2.62 Å EMDB-26464: Cryo-EM structure of the rigor state Jordan myosin-15-F-actin complex (symmetry expansion) 手法: EM (単粒子) / 解像度: 4.18 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	gallus gallus (ニワトリ) chicken (ニワトリ) mus musculus (ハツカネズミ)
キーワード	CELL ADHESION (細胞接着) / filamentous actin / cytoskeleton (細胞骨格) / adhesion (接着) / contractility / MOTOR PROTEIN (モータータンパク質) / myosin motor proteins / actin cytoskeleton (マイクロフィラメント) / stereocilia / deafness (難聴) / MOTOR PROTEIN/ATP Binding Protein (機関 (機械)) / MOTOR PROTEIN-ATP Binding Protein complex (機関 (機械))