EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural mechanism of GTPase-powered ribosome-tRNA movement.
ジャーナル・号・ページ	Nat Commun, Vol. 12, Issue 1, Page 5933, Year 2021
掲載日	2021年10月11日
著者	Valentyn Petrychenko / Bee-Zen Peng / Ana C de A P Schwarzer / Frank Peske / Marina V Rodnina / Niels Fischer /
PubMed 要旨	GTPases are regulators of cell signaling acting as molecular switches. The translational GTPase EF-G stands out, as it uses GTP hydrolysis to generate force and promote the movement of the ribosome ...GTPases are regulators of cell signaling acting as molecular switches. The translational GTPase EF-G stands out, as it uses GTP hydrolysis to generate force and promote the movement of the ribosome along the mRNA. The key unresolved question is how GTP hydrolysis drives molecular movement. Here, we visualize the GTPase-powered step of ongoing translocation by time-resolved cryo-EM. EF-G in the active GDP-Pi form stabilizes the rotated conformation of ribosomal subunits and induces twisting of the sarcin-ricin loop of the 23 S rRNA. Refolding of the GTPase switch regions upon Pi release initiates a large-scale rigid-body rotation of EF-G pivoting around the sarcin-ricin loop that facilitates back rotation of the ribosomal subunits and forward swiveling of the head domain of the small subunit, ultimately driving tRNA forward movement. The findings demonstrate how a GTPase orchestrates spontaneous thermal fluctuations of a large RNA-protein complex into force-generating molecular movement.
リンク	Nat Commun / PubMed:34635670 / PubMed Central
手法	EM (単粒子)
解像度	2.35 - 9.5 Å
構造データ	13458 7pjs EMDB-13458, PDB-7pjs: Structure of the 70S ribosome with tRNAs in the classical pre-translocation state and apramycin (C) 手法: EM (単粒子) / 解像度: 2.35 Å 13459 7pjt EMDB-13459, PDB-7pjt: Structure of the 70S ribosome with tRNAs in hybrid state 1 (H1) 手法: EM (単粒子) / 解像度: 6.0 Å 13460 7pju EMDB-13460, PDB-7pju: Structure of the 70S ribosome with tRNAs in hybrid state 2 (H2) 手法: EM (単粒子) / 解像度: 9.5 Å 13461 7pjv EMDB-13461, PDB-7pjv: Structure of the 70S-EF-G-GDP-Pi ribosome complex with tRNAs in hybrid state 1 (H1-EF-G-GDP-Pi) 手法: EM (単粒子) / 解像度: 3.1 Å 13462 7pjw EMDB-13462, PDB-7pjw: Structure of the 70S-EF-G-GDP-Pi ribosome complex with tRNAs in hybrid state 2 (H2-EF-G-GDP-Pi) 手法: EM (単粒子) / 解像度: 4.0 Å 13463 7pjx EMDB-13463, PDB-7pjx: Structure of the 70S-EF-G-GDP ribosome complex with tRNAs in hybrid state 1 (H1-EF-G-GDP) 手法: EM (単粒子) / 解像度: 6.5 Å 13464 7pjy EMDB-13464, PDB-7pjy: Structure of the 70S-EF-G-GDP ribosome complex with tRNAs in chimeric state 1 (CHI1-EF-G-GDP) 手法: EM (単粒子) / 解像度: 3.1 Å 13465 7pjz EMDB-13465, PDB-7pjz: Structure of the 70S-EF-G-GDP ribosome complex with tRNAs in chimeric state 2 (CHI2-EF-G-GDP) 手法: EM (単粒子) / 解像度: 6.0 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-NA: Unknown entry / ナトリウムカチオン ChemComp-AM2: APRAMYCIN / 抗生剤YM ChemComp-HOH: WATER ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM ChemComp-PO4: PHOSPHATE ION / ホスファ-ト
由来	escherichia coli (大腸菌) escherichia coli (strain k12) (大腸菌)
キーワード	RIBOSOME / EF-G / robosome / 70S / apramycin / translocation