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-Structure paper
タイトル | Structures of outer-arm dynein array on microtubule doublet reveal a motor coordination mechanism. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 28, Issue 10, Page 799-810, Year 2021 |
掲載日 | 2021年9月23日 |
著者 | Qinhui Rao / Long Han / Yue Wang / Pengxin Chai / Yin-Wei Kuo / Renbin Yang / Fangheng Hu / Yuchen Yang / Jonathon Howard / Kai Zhang / |
PubMed 要旨 | Thousands of outer-arm dyneins (OADs) are arrayed in the axoneme to drive a rhythmic ciliary beat. Coordination among multiple OADs is essential for generating mechanical forces to bend microtubule ...Thousands of outer-arm dyneins (OADs) are arrayed in the axoneme to drive a rhythmic ciliary beat. Coordination among multiple OADs is essential for generating mechanical forces to bend microtubule doublets (MTDs). Using electron microscopy, we determined high-resolution structures of Tetrahymena thermophila OAD arrays bound to MTDs in two different states. OAD preferentially binds to MTD protofilaments with a pattern resembling the native tracks for its distinct microtubule-binding domains. Upon MTD binding, free OADs are induced to adopt a stable parallel conformation, primed for array formation. Extensive tail-to-head (TTH) interactions between OADs are observed, which need to be broken for ATP turnover by the dynein motor. We propose that OADs in an array sequentially hydrolyze ATP to slide the MTDs. ATP hydrolysis in turn relaxes the TTH interfaces to effect free nucleotide cycles of downstream OADs. These findings lead to a model explaining how conformational changes in the axoneme produce coordinated action of dyneins. |
リンク | Nat Struct Mol Biol / PubMed:34556869 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.5 - 8.0 Å |
構造データ | EMDB-22677, PDB-7k58: EMDB-22679, PDB-7k5b: EMDB-22840, PDB-7kek: EMDB-24066, PDB-7mwg: |
化合物 | ChemComp-ADP: ChemComp-ATP: ChemComp-MG: ChemComp-GTP: ChemComp-GDP: ChemComp-HOH: |
由来 |
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キーワード | STRUCTURAL PROTEIN / outer-arm dynein / axonemal dynein / microtubule doublet / MOTOR PROTEIN / three head / outer dynein arms / microtubule binding / OAD / axonemal / pre-paralle / protofilament of microtubule doublet |