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-Structure paper
タイトル | Molecular structure of the intact bacterial flagellar basal body. |
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ジャーナル・号・ページ | Nat Microbiol, Vol. 6, Issue 6, Page 712-721, Year 2021 |
掲載日 | 2021年4月30日 |
著者 | Steven Johnson / Emily J Furlong / Justin C Deme / Ashley L Nord / Joseph J E Caesar / Fabienne F V Chevance / Richard M Berry / Kelly T Hughes / Susan M Lea / |
PubMed 要旨 | The bacterial flagellum is a macromolecular protein complex that enables motility in many species. Bacterial flagella self-assemble a strong, multicomponent drive shaft that couples rotation in the ...The bacterial flagellum is a macromolecular protein complex that enables motility in many species. Bacterial flagella self-assemble a strong, multicomponent drive shaft that couples rotation in the inner membrane to the micrometre-long flagellar filament that powers bacterial swimming in viscous fluids. Here, we present structures of the intact Salmonella flagellar basal body, encompassing the inner membrane rotor, drive shaft and outer-membrane bushing, solved using cryo-electron microscopy to resolutions of 2.2-3.7 Å. The structures reveal molecular details of how 173 protein molecules of 13 different types assemble into a complex spanning two membranes and a cell wall. The helical drive shaft at one end is intricately interwoven with the rotor component with both the export gate complex and the proximal rod forming interactions with the MS-ring. At the other end, the drive shaft distal rod passes through the LP-ring bushing complex, which functions as a molecular bearing anchored in the outer membrane through interactions with the lipopolysaccharide. The in situ structure of a protein complex capping the drive shaft provides molecular insights into the assembly process of this molecular machine. |
リンク | Nat Microbiol / PubMed:33931760 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.2 - 3.8 Å |
構造データ | EMDB-12183, PDB-7bgl: EMDB-12190, PDB-7bhq: EMDB-12192, PDB-7bin: EMDB-12193, PDB-7bj2: EMDB-12195, PDB-7bk0: EMDB-12603, PDB-7nvg: |
化合物 | ChemComp-TLW: ChemComp-TQN: |
由来 |
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キーワード | MEMBRANE PROTEIN / bacterial flagellum LP ring salmonella / bacterial flagellum hook cap salmonella flagellar assembly / PROTEIN TRANSPORT / bacterial flagellum rod bacterial flagellum export gate basal body / bacterial flagellum basal body MS-ring / bacterial flagellum / flagella / basal body |