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-Structure paper
タイトル | The structure of human CST reveals a decameric assembly bound to telomeric DNA. |
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ジャーナル・号・ページ | Science, Vol. 368, Issue 6495, Page 1081-1085, Year 2020 |
掲載日 | 2020年6月5日 |
![]() | Ci Ji Lim / Alexandra T Barbour / Arthur J Zaug / Karen J Goodrich / Allison E McKay / Deborah S Wuttke / Thomas R Cech / ![]() |
PubMed 要旨 | The CTC1-STN1-TEN1 (CST) complex is essential for telomere maintenance and resolution of stalled replication forks genome-wide. Here, we report the 3.0-angstrom cryo-electron microscopy structure of ...The CTC1-STN1-TEN1 (CST) complex is essential for telomere maintenance and resolution of stalled replication forks genome-wide. Here, we report the 3.0-angstrom cryo-electron microscopy structure of human CST bound to telomeric single-stranded DNA (ssDNA), which assembles as a decameric supercomplex. The atomic model of the 134-kilodalton CTC1 subunit, built almost entirely de novo, reveals the overall architecture of CST and the DNA-binding anchor site. The carboxyl-terminal domain of STN1 interacts with CTC1 at two separate docking sites, allowing allosteric mediation of CST decamer assembly. Furthermore, ssDNA appears to staple two monomers to nucleate decamer assembly. CTC1 has stronger structural similarity to Replication Protein A than the expected similarity to yeast Cdc13. The decameric structure suggests that CST can organize ssDNA analogously to the nucleosome's organization of double-stranded DNA. |
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手法 | EM (単粒子) |
解像度 | 3.0 - 9.2 Å |
構造データ | ![]() EMDB-21561: ![]() EMDB-21563: ![]() EMDB-21565: ![]() EMDB-21566: EMDB-21567, PDB-6w6w: |
化合物 | ![]() ChemComp-ZN: |
由来 |
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![]() | STRUCTURAL PROTEIN/DNA / Telomere homeostasis / telomere packaging / telomerase terminator / DNA replication / Double-stranded breaks repair / single-stranded DNA-binding proteins / higher-order protein assembly / DNA-induced oligomeriization / STRUCTURAL PROTEIN / STRUCTURAL PROTEIN-DNA complex |