EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Multiple conformations facilitate PilT function in the type IV pilus.
ジャーナル・号・ページ	Nat Commun, Vol. 10, Issue 1, Page 5198, Year 2019
掲載日	2019年11月15日
著者	Matthew McCallum / Samir Benlekbir / Sheryl Nguyen / Stephanie Tammam / John L Rubinstein / Lori L Burrows / P Lynne Howell /
PubMed 要旨	Type IV pilus-like systems are protein complexes that polymerize pilin fibres. They are critical for virulence in many bacterial pathogens. Pilin polymerization and depolymerization are powered by ...Type IV pilus-like systems are protein complexes that polymerize pilin fibres. They are critical for virulence in many bacterial pathogens. Pilin polymerization and depolymerization are powered by motor ATPases of the PilT/VirB11-like family. This family is thought to operate with C symmetry; however, most of these ATPases crystallize with either C or C symmetric conformations. The relevance of these conformations is unclear. Here, we determine the X-ray structures of PilT in four unique conformations and use these structures to classify the conformation of available PilT/VirB11-like family member structures. Single particle electron cryomicroscopy (cryoEM) structures of PilT reveal condition-dependent preferences for C C, and C conformations. The physiologic importance of these conformations is validated by coevolution analysis and functional studies of point mutants, identifying a rare gain-of-function mutation that favours the C conformation. With these data, we propose a comprehensive model of PilT function with broad implications for PilT/VirB11-like family members.
リンク	Nat Commun / PubMed:31729381 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.892 - 7.8 Å
構造データ	20114 6olj EMDB-20114, PDB-6olj: CryoEM structure of PilB from Geobacter metallireducens: C2ccocco conformation 手法: EM (単粒子) / 解像度: 7.8 Å 20115 6olk EMDB-20115, PDB-6olk: CryoEM structure of PilT4 from Geobacter metallireducens without adding nucleotide: C3ocococ conformation 手法: EM (単粒子) / 解像度: 4.0 Å 20116 6oll EMDB-20116, PDB-6oll: CryoEM structure of PilT4 from Geobacter metallireducens without adding nucleotide: C2oocooc conformation 手法: EM (単粒子) / 解像度: 4.1 Å 20117 6olm EMDB-20117, PDB-6olm: CryoEM structure of PilT4 from Geobacter metallireducens with added ATP: C6cccccc conformation 手法: EM (単粒子) / 解像度: 4.4 Å PDB-6ojx: PilT4 from Geobacter metallireducens bound to ATP 手法: X-RAY DIFFRACTION / 解像度: 1.892 Å PDB-6ojy: Methylated PilT4 from Geobacter metallireducens bound to sulfate: C3ocococ conformation 手法: X-RAY DIFFRACTION / 解像度: 3.3 Å PDB-6ojz: PilT4 from Geobacter metallireducens bound to ADP with partial occupancy: C3ocococ conformation 手法: X-RAY DIFFRACTION / 解像度: 3.027 Å PDB-6ok2: PilT4 from Geobacter metallireducens bound to ADP: C3ocococ conformation 手法: X-RAY DIFFRACTION / 解像度: 3.287 Å PDB-6okv: PilT4 from Geobacter metallireducens bound to AMP-PNP: C2ccocco conformation 手法: X-RAY DIFFRACTION / 解像度: 4.007 Å
化合物	ChemComp-EDO: 1,2-ETHANEDIOL / エチレングリコ-ル ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-HOH: WATER ChemComp-SO4: SULFATE ION / 硫酸ジアニオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP / AMP-PNP, エネルギー貯蔵分子類似体*YM
由来	Geobacter metallireducens (バクテリア) geobacter metallireducens (strain gs-15 / atcc 53774 / dsm 7210) (バクテリア)
キーワード	MOTOR PROTEIN / T4P / ATPase / type IV pilus / motor