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-Structure paper
タイトル | Cryo-EM structure of a functional monomeric Photosystem I from Thermosynechococcus elongatus reveals red chlorophyll cluster. |
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ジャーナル・号・ページ | Commun Biol, Vol. 4, Issue 1, Page 304, Year 2021 |
掲載日 | 2021年3月8日 |
著者 | Orkun Çoruh / Anna Frank / Hideaki Tanaka / Akihiro Kawamoto / Eithar El-Mohsnawy / Takayuki Kato / Keiichi Namba / Christoph Gerle / Marc M Nowaczyk / Genji Kurisu / |
PubMed 要旨 | A high-resolution structure of trimeric cyanobacterial Photosystem I (PSI) from Thermosynechococcus elongatus was reported as the first atomic model of PSI almost 20 years ago. However, the monomeric ...A high-resolution structure of trimeric cyanobacterial Photosystem I (PSI) from Thermosynechococcus elongatus was reported as the first atomic model of PSI almost 20 years ago. However, the monomeric PSI structure has not yet been reported despite long-standing interest in its structure and extensive spectroscopic characterization of the loss of red chlorophylls upon monomerization. Here, we describe the structure of monomeric PSI from Thermosynechococcus elongatus BP-1. Comparison with the trimer structure gave detailed insights into monomerization-induced changes in both the central trimerization domain and the peripheral regions of the complex. Monomerization-induced loss of red chlorophylls is assigned to a cluster of chlorophylls adjacent to PsaX. Based on our findings, we propose a role of PsaX in the stabilization of red chlorophylls and that lipids of the surrounding membrane present a major source of thermal energy for uphill excitation energy transfer from red chlorophylls to P700. |
リンク | Commun Biol / PubMed:33686186 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 3.2 - 6.5 Å |
構造データ | EMDB-0977, PDB-6lu1: PDB-7bw2: |
化合物 | ChemComp-CLA: ChemComp-PQN: ChemComp-BCR: ChemComp-LHG: ChemComp-SF4: ChemComp-LMG: ChemComp-HOH: |
由来 |
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キーワード | MEMBRANE PROTEIN / monomer / complex / photosystem / photosynthesis |