EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Enterovirus particles expel capsid pentamers to enable genome release.
ジャーナル・号・ページ	Nat Commun, Vol. 10, Issue 1, Page 1138, Year 2019
掲載日	2019年3月8日
著者	David Buchta / Tibor Füzik / Dominik Hrebík / Yevgen Levdansky / Lukáš Sukeník / Liya Mukhamedova / Jana Moravcová / Robert Vácha / Pavel Plevka /
PubMed 要旨	Viruses from the genus Enterovirus are important human pathogens. Receptor binding or exposure to acidic pH in endosomes converts enterovirus particles to an activated state that is required for ...Viruses from the genus Enterovirus are important human pathogens. Receptor binding or exposure to acidic pH in endosomes converts enterovirus particles to an activated state that is required for genome release. However, the mechanism of enterovirus uncoating is not well understood. Here, we use cryo-electron microscopy to visualize virions of human echovirus 18 in the process of genome release. We discover that the exit of the RNA from the particle of echovirus 18 results in a loss of one, two, or three adjacent capsid-protein pentamers. The opening in the capsid, which is more than 120 Å in diameter, enables the release of the genome without the need to unwind its putative double-stranded RNA segments. We also detect capsids lacking pentamers during genome release from echovirus 30. Thus, our findings uncover a mechanism of enterovirus genome release that could become target for antiviral drugs.
リンク	Nat Commun / PubMed:30850609 / PubMed Central
手法	EM (単粒子)
解像度	3.16 - 19.4 Å
構造データ	0181 6hbg EMDB-0181, PDB-6hbg: Echovirus 18 native particle 手法: EM (単粒子) / 解像度: 3.16 Å 0182 6hbh EMDB-0182, PDB-6hbh: Echovirus 18 A-particle 手法: EM (単粒子) / 解像度: 3.36 Å 0183 6hbj EMDB-0183, PDB-6hbj: Echovirus 18 empty particle 手法: EM (単粒子) / 解像度: 3.16 Å EMDB-0184: Echovirus 18 Open particle without one pentamer 手法: EM (単粒子) / 解像度: 8.8 Å 0185 6hbk EMDB-0185, PDB-6hbk: Echovirus 18 Open particle without one pentamer 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-0186: Echovirus 18 Open particle without two pentamers 手法: EM (単粒子) / 解像度: 6.62 Å EMDB-0187: Echovirus 18 Open particle without three pentamers 手法: EM (単粒子) / 解像度: 4.85 Å 0188 6hbl EMDB-0188, PDB-6hbl: Echovirus 18 Open particle without three pentamers 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-0189: Echovirus 30 Open particle without one pentamer 手法: EM (単粒子) / 解像度: 19.4 Å 0217 6hht EMDB-0217, PDB-6hht: Echovirus 18 Open particle without two pentamers 手法: EM (単粒子) / 解像度: 4.05 Å
化合物	ChemComp-PLM: PALMITIC ACID / パルミチン酸 ChemComp-GUN: GUANINE / グアニン
由来	echovirus e18 (ウイルス)
キーワード	VIRUS / echovirus / echovirus 18 / native particle / enterovirus / picornavirus / altered particle / A-particle / empty particle / B-particle / open particle / O-particle / genome release