|Entry||Database: EMDB / Id: 189|
|Title||Echovirus 30 Open particle without one pentamer|
|Map data||Echovirus 30 Open particle without one pentamer|
|Method||single particle reconstruction / cryo EM / Resolution: 19.4 Å|
|Authors||Mukhamedova L / Buchta D / Fuzik T / Hrebik D / Levdansky Y / Moravcova J / Plevka P|
|Citation||Journal: Nat Commun / Year: 2019|
Title: Enterovirus particles expel capsid pentamers to enable genome release.
P-authors: David Buchta / Tibor Füzik / Dominik Hrebík / Yevgen Levdansky / Lukáš Sukeník / Liya Mukhamedova / Jana Moravcová / Robert Vácha / Pavel Plevka /
Abstract: Viruses from the genus Enterovirus are important human pathogens. Receptor binding or exposure to acidic pH in endosomes converts enterovirus particles to an activated state that is required for ...Viruses from the genus Enterovirus are important human pathogens. Receptor binding or exposure to acidic pH in endosomes converts enterovirus particles to an activated state that is required for genome release. However, the mechanism of enterovirus uncoating is not well understood. Here, we use cryo-electron microscopy to visualize virions of human echovirus 18 in the process of genome release. We discover that the exit of the RNA from the particle of echovirus 18 results in a loss of one, two, or three adjacent capsid-protein pentamers. The opening in the capsid, which is more than 120 Å in diameter, enables the release of the genome without the need to unwind its putative double-stranded RNA segments. We also detect capsids lacking pentamers during genome release from echovirus 30. Thus, our findings uncover a mechanism of enterovirus genome release that could become target for antiviral drugs.
|Date||Deposition: Aug 10, 2018 / Header (metadata) release: Oct 24, 2018 / Map release: Mar 20, 2019 / Last update: Mar 20, 2019|
|Structure viewer||EM map: |
|File||emd_0189.map.gz (map file in CCP4 format, 536871 KB)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 1.063 Å|
CCP4 map header:
-Entire Echovirus E30
|Entire||Name: Echovirus E30 / Number of components: 1|
-Component #1: virus, Echovirus E30
|Virus||Name: Echovirus E30 / Class: VIRION / Empty: Yes / Enveloped: No / Isolate: STRAIN|
|Species||Species: Echovirus E30 / Strain: Bastianni|
|Source (natural)||Host Species: Homo sapiens (human)|
|Shell #1||Name of element: Capsid / Diameter: 340.0 Å / T number(triangulation number): 1|
|Specimen||Specimen state: particle / Method: cryo EM|
|Sample solution||Specimen conc.: 3 mg/ml / Ph: 7.4|
|Vitrification||Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Temperature: 298 K / Humidity: 100 %|
-Electron microscopy imaging
Model: Titan Krios / Image courtesy: FEI Company
|Imaging||Microscope: FEI TITAN KRIOS|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 45 e/Å2 / Illumination mode: FLOOD BEAM|
|Lens||Magnification: 75000.0 X (nominal), 79725.0 X (calibrated) / Cs: 2.7 mm / Imaging mode: BRIGHT FIELD / Defocus: 1000.0 - 3000.0 nm|
|Specimen Holder||Model: FEI TITAN KRIOS AUTOGRID HOLDER|
|Camera||Detector: FEI FALCON II (4k x 4k)|
|Image acquisition||Number of digital images: 1283 / Sampling size: 14 microns|
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