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Structure paper

TitleEnterovirus particles expel capsid pentamers to enable genome release.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 1138, Year 2019
Publish dateMar 8, 2019
AuthorsDavid Buchta / Tibor Füzik / Dominik Hrebík / Yevgen Levdansky / Lukáš Sukeník / Liya Mukhamedova / Jana Moravcová / Robert Vácha / Pavel Plevka /
PubMed AbstractViruses from the genus Enterovirus are important human pathogens. Receptor binding or exposure to acidic pH in endosomes converts enterovirus particles to an activated state that is required for ...Viruses from the genus Enterovirus are important human pathogens. Receptor binding or exposure to acidic pH in endosomes converts enterovirus particles to an activated state that is required for genome release. However, the mechanism of enterovirus uncoating is not well understood. Here, we use cryo-electron microscopy to visualize virions of human echovirus 18 in the process of genome release. We discover that the exit of the RNA from the particle of echovirus 18 results in a loss of one, two, or three adjacent capsid-protein pentamers. The opening in the capsid, which is more than 120 Å in diameter, enables the release of the genome without the need to unwind its putative double-stranded RNA segments. We also detect capsids lacking pentamers during genome release from echovirus 30. Thus, our findings uncover a mechanism of enterovirus genome release that could become target for antiviral drugs.
External linksNat Commun / PubMed:30850609 / PubMed Central
MethodsEM (single particle)
Resolution3.16 - 19.4 Å
Structure data

0181

6hbg

EMDB-0181, PDB-6hbg:
Echovirus 18 native particle
Method: EM (single particle) / Resolution: 3.16 Å

0182

6hbh

EMDB-0182, PDB-6hbh:
Echovirus 18 A-particle
Method: EM (single particle) / Resolution: 3.36 Å

0183

6hbj

EMDB-0183, PDB-6hbj:
Echovirus 18 empty particle
Method: EM (single particle) / Resolution: 3.16 Å

EMDB-0184:
Echovirus 18 Open particle without one pentamer
Method: EM (single particle) / Resolution: 8.8 Å

0185

6hbk

EMDB-0185, PDB-6hbk:
Echovirus 18 Open particle without one pentamer
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-0186:
Echovirus 18 Open particle without two pentamers
Method: EM (single particle) / Resolution: 6.62 Å

EMDB-0187:
Echovirus 18 Open particle without three pentamers
Method: EM (single particle) / Resolution: 4.85 Å

0188

6hbl

EMDB-0188, PDB-6hbl:
Echovirus 18 Open particle without three pentamers
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-0189:
Echovirus 30 Open particle without one pentamer
Method: EM (single particle) / Resolution: 19.4 Å

0217

6hht

EMDB-0217, PDB-6hht:
Echovirus 18 Open particle without two pentamers
Method: EM (single particle) / Resolution: 4.05 Å

Chemicals

ChemComp-PLM:
PALMITIC ACID / Palmitic acid

ChemComp-GUN:
GUANINE / Guanine

Source
  • echovirus e18
KeywordsVIRUS / echovirus / echovirus 18 / native particle / enterovirus / picornavirus / altered particle / A-particle / empty particle / B-particle / open particle / O-particle / genome release

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