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-Structure paper
タイトル | MicroED structure of the NaK ion channel reveals a Na partition process into the selectivity filter. |
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ジャーナル・号・ページ | Commun Biol, Vol. 1, Page 38, Year 2018 |
掲載日 | 2018年5月3日 |
![]() | Shian Liu / Tamir Gonen / ![]() |
PubMed 要旨 | Sodium (Na) is a ubiquitous and important inorganic salt mediating many critical biological processes such as neuronal excitation, signaling, and facilitation of various transporters. The hydration ...Sodium (Na) is a ubiquitous and important inorganic salt mediating many critical biological processes such as neuronal excitation, signaling, and facilitation of various transporters. The hydration states of Na are proposed to play critical roles in determining the conductance and the selectivity of Na channels, yet they are rarely captured by conventional structural biology means. Here we use the emerging cryo-electron microscopy (cryoEM) method micro-electron diffraction (MicroED) to study the structure of a prototypical tetrameric Na-conducting channel, NaK, to 2.5 Å resolution from nano-crystals. Two new conformations at the external site of NaK are identified, allowing us to visualize a partially hydrated Na ion at the entrance of the channel pore. A process of dilation coupled with Na movement is identified leading to valuable insights into the mechanism of ion conduction and gating. This study lays the ground work for future studies using MicroED in membrane protein biophysics. |
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手法 | EM (電子線結晶学) |
解像度 | 2.5002 Å |
構造データ | |
化合物 | ![]() ChemComp-NA: ![]() ChemComp-MPD: ![]() ChemComp-HOH: |
由来 |
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![]() | TRANSPORT PROTEIN / ion channel / NaK |