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TitleMicroED structure of the NaK ion channel reveals a Na partition process into the selectivity filter.
Journal, issue, pagesCommun Biol, Vol. 1, Page 38, Year 2018
Publish dateMay 3, 2018
AuthorsShian Liu / Tamir Gonen /
PubMed AbstractSodium (Na) is a ubiquitous and important inorganic salt mediating many critical biological processes such as neuronal excitation, signaling, and facilitation of various transporters. The hydration ...Sodium (Na) is a ubiquitous and important inorganic salt mediating many critical biological processes such as neuronal excitation, signaling, and facilitation of various transporters. The hydration states of Na are proposed to play critical roles in determining the conductance and the selectivity of Na channels, yet they are rarely captured by conventional structural biology means. Here we use the emerging cryo-electron microscopy (cryoEM) method micro-electron diffraction (MicroED) to study the structure of a prototypical tetrameric Na-conducting channel, NaK, to 2.5 Å resolution from nano-crystals. Two new conformations at the external site of NaK are identified, allowing us to visualize a partially hydrated Na ion at the entrance of the channel pore. A process of dilation coupled with Na movement is identified leading to valuable insights into the mechanism of ion conduction and gating. This study lays the ground work for future studies using MicroED in membrane protein biophysics.
External linksCommun Biol / PubMed:30167468 / PubMed Central
MethodsEM (electron crystallography)
Resolution2.5002 Å
Structure data

7558

6cpv

EMDB-7558, PDB-6cpv:
MicroED structure of NaK ion channel reveals a process of Na+ partition into the selectivity filter
Method: EM (electron crystallography) / Resolution: 2.5002 Å

Chemicals

ChemComp-NA:
Unknown entry

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-HOH:
WATER

Source
  • bacillus cereus (bacteria)
KeywordsTRANSPORT PROTEIN / ion channel / NaK

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