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-Structure paper
タイトル | Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED. |
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ジャーナル・号・ページ | Nat Methods, Vol. 14, Issue 4, Page 399-402, Year 2017 |
掲載日 | 2017年2月13日 |
![]() | M Jason de la Cruz / Johan Hattne / Dan Shi / Paul Seidler / Jose Rodriguez / Francis E Reyes / Michael R Sawaya / Duilio Cascio / Simon C Weiss / Sun Kyung Kim / Cynthia S Hinck / Andrew P Hinck / Guillermo Calero / David Eisenberg / Tamir Gonen / ![]() |
PubMed 要旨 | Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from ...Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals into microcrystals or nanocrystals can provide a simple path for high-resolution structure determination by the cryoEM method MicroED and potentially by serial femtosecond crystallography. |
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手法 | EM (電子線結晶学) |
解像度 | 1.1 - 2.9 Å |
構造データ | EMDB-8216, PDB-5k7n: EMDB-8221, PDB-5k7s: |
化合物 | ![]() ChemComp-HOH: ![]() ChemComp-CL: ![]() ChemComp-NA: ![]() ChemComp-IOD: ![]() ChemComp-CA: ![]() ChemComp-ZN: ![]() ChemComp-DMS: ![]() ChemComp-IPA: |
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![]() | PROTEIN FIBRIL / ![]() ![]() ![]() ![]() ![]() |