EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
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IF	>30 >20 >10 >5 all

タイトル	Complexity and ultrastructure of infectious extracellular vesicles from cells infected by non-enveloped virus.
ジャーナル・号・ページ	Sci Rep, Vol. 10, Issue 1, Page 7939, Year 2020
掲載日	2020年5月14日
著者	Jie E Yang / Evan D Rossignol / Deborah Chang / Joseph Zaia / Isaac Forrester / Kiran Raja / Holly Winbigler / Daniela Nicastro / William T Jackson / Esther Bullitt /
PubMed 要旨	Enteroviruses support cell-to-cell viral transmission prior to their canonical lytic spread of virus. Poliovirus (PV), a prototype for human pathogenic positive-sense RNA enteroviruses, and ...Enteroviruses support cell-to-cell viral transmission prior to their canonical lytic spread of virus. Poliovirus (PV), a prototype for human pathogenic positive-sense RNA enteroviruses, and picornaviruses in general, transport multiple virions en bloc via infectious extracellular vesicles, 100~1000 nm in diameter, secreted from host cells. Using biochemical and biophysical methods we identify multiple components in secreted microvesicles, including mature PV virions; positive-sense genomic and negative-sense replicative, template viral RNA; essential viral replication proteins; and cellular proteins. Using cryo-electron tomography, we visualize the near-native three-dimensional architecture of secreted infectious microvesicles containing both virions and a unique morphological component that we describe as a mat-like structure. While the composition of these mat-like structures is not yet known, based on our biochemical data they are expected to be comprised of unencapsidated RNA and proteins. In addition to infectious microvesicles, CD9-positive exosomes released from PV-infected cells are also infectious and transport virions. Thus, our data show that, prior to cell lysis, non-enveloped viruses are secreted within infectious vesicles that also transport viral unencapsidated RNAs, viral and host proteins. Understanding the structure and function of these infectious particles helps elucidate the mechanism by which extracellular vesicles contribute to the spread of non-enveloped virus infection.
リンク	Sci Rep / PubMed:32409751 / PubMed Central
手法	EM (トモグラフィー)
構造データ	EMDB-7871: Class 3, Infectious Microvesicles from poliovirus-infected HeLa cells 手法: EM (トモグラフィー) EMDB-7872: Class 2, Infectious Microvesicles from poliovirus-infected HeLa cells 手法: EM (トモグラフィー) EMDB-7873: Class 1, Infectious Microvesicles from poliovirus-infected HeLa cells 手法: EM (トモグラフィー) EMDB-7877: Noodle-like structures in infectious microvesicles from poliovirus-infected HeLa cells 手法: EM (トモグラフィー) EMDB-7878: Noodle-like structure in infectious microvesicles (Class 3) from poliovirus-infected HeLa cells 手法: EM (トモグラフィー) EMDB-7879: Infectious exosomes from poliovirus-infected HeLa cells 手法: EM (トモグラフィー) EMDB-7880: Infectious microvesicles (Class 1 and 3) from poliovirus-infected HeLa cells, displaying clusters of virions and additional inner vesicular structures 手法: EM (トモグラフィー) EMDB-7881: Subcellular fractionation of viral replication membranes from poliovirus-infected HeLa cells at 5 hours post infection 手法: EM (トモグラフィー)