National Institutes of Health/National Institute of General Medical Sciences
R01 GM102474
United States
National Institutes of Health/National Institute of General Medical Sciences
U24GM116787
United States
Citation
Journal: Sci Rep / Year: 2020 Title: Complexity and ultrastructure of infectious extracellular vesicles from cells infected by non-enveloped virus. Authors: Jie E Yang / Evan D Rossignol / Deborah Chang / Joseph Zaia / Isaac Forrester / Kiran Raja / Holly Winbigler / Daniela Nicastro / William T Jackson / Esther Bullitt / Abstract: Enteroviruses support cell-to-cell viral transmission prior to their canonical lytic spread of virus. Poliovirus (PV), a prototype for human pathogenic positive-sense RNA enteroviruses, and ...Enteroviruses support cell-to-cell viral transmission prior to their canonical lytic spread of virus. Poliovirus (PV), a prototype for human pathogenic positive-sense RNA enteroviruses, and picornaviruses in general, transport multiple virions en bloc via infectious extracellular vesicles, 100~1000 nm in diameter, secreted from host cells. Using biochemical and biophysical methods we identify multiple components in secreted microvesicles, including mature PV virions; positive-sense genomic and negative-sense replicative, template viral RNA; essential viral replication proteins; and cellular proteins. Using cryo-electron tomography, we visualize the near-native three-dimensional architecture of secreted infectious microvesicles containing both virions and a unique morphological component that we describe as a mat-like structure. While the composition of these mat-like structures is not yet known, based on our biochemical data they are expected to be comprised of unencapsidated RNA and proteins. In addition to infectious microvesicles, CD9-positive exosomes released from PV-infected cells are also infectious and transport virions. Thus, our data show that, prior to cell lysis, non-enveloped viruses are secreted within infectious vesicles that also transport viral unencapsidated RNAs, viral and host proteins. Understanding the structure and function of these infectious particles helps elucidate the mechanism by which extracellular vesicles contribute to the spread of non-enveloped virus infection.
Download / File: emd_7872.map.gz / Format: CCP4 / Size: 41.5 MB / Type: IMAGE STORED AS SIGNED BYTE
Annotation
Virion-containing microvesicles from poliovirus-infected cells, Class 2
Voxel size
X=Y=Z: 8.28 Å
Density
Minimum - Maximum
-117 - 101
Average (Standard dev.)
25.824907 (±4.59721)
Symmetry
Space group: 1
Details
EMDB XML:
Map geometry
Axis order
X
Y
Z
Origin
0
0
0
Dimensions
594
488
150
Spacing
488
594
150
Cell
A: 4040.64 Å / B: 4918.32 Å / C: 1242.0 Å α=β=γ: 90.0 °
CCP4 map header:
mode
envelope stored as signed bytes (from -128 lowest to 127 highest)
Å/pix. X/Y/Z
8.28
8.28
8.28
M x/y/z
488
594
150
origin x/y/z
0.000
0.000
0.000
length x/y/z
4040.640
4918.320
1242.000
α/β/γ
90.000
90.000
90.000
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
0
NC/NR/NS
488
594
150
D min/max/mean
-117.000
101.000
25.825
-
Supplemental data
-
Sample components
-
Entire : Human poliovirus 1 Mahoney
Entire
Name: Human poliovirus 1 Mahoney
Components
Virus: Human poliovirus 1 Mahoney
-
Supramolecule #1: Human poliovirus 1 Mahoney
Supramolecule
Name: Human poliovirus 1 Mahoney / type: virus / ID: 1 / Parent: 0 Details: virion-containing microvesicles collected from supernatant of poliovirus-infected cells at 8 hours post-infection NCBI-ID: 12081 / Sci species name: Human poliovirus 1 Mahoney / Virus type: VIRUS-LIKE PARTICLE / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: No
-
Experimental details
-
Structure determination
Method
cryo EM
Processing
electron tomography
Aggregation state
particle
-
Sample preparation
Concentration
1.5 mg/mL
Buffer
pH: 7 / Details: 1% glutaraldehyde in 1x PBS
Grid
Material: COPPER / Mesh: 200
Vitrification
Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 283.15 K / Instrument: FEI VITROBOT MARK II
Details
The microvesicles from poliovirus-infected cells were collected through a series of centrifugation to enrich 100-1000 nm diameter membrane particles, followed by an annexin-V column purification to enrich phosphatidylserine-containing microvesicles.
Sectioning
Other: NO SECTIONING
Fiducial marker
Manufacturer: TED PELLA INC / Diameter: 10 nm
-
Electron microscopy
Microscope
FEI TECNAI F20
Image recording
Film or detector model: TVIPS TEMCAM-F416 (4k x 4k) / Average electron dose: 4.8 e/Å2
Electron beam
Acceleration voltage: 160 kV / Electron source: FIELD EMISSION GUN
Electron optics
Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Tecnai F20 / Image courtesy: FEI Company
-
Image processing
Final reconstruction
Algorithm: BACK PROJECTION / Software - Name: eTomo / Number images used: 55
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