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-Structure paper
タイトル | Structures of Mature and Urea-Treated Empty Bacteriophage T5: Insights into Siphophage Infection and DNA Ejection. |
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ジャーナル・号・ページ | Int J Mol Sci, Vol. 25, Issue 15, Year 2024 |
掲載日 | 2024年8月3日 |
著者 | Yuning Peng / Huanrong Tang / Hao Xiao / Wenyuan Chen / Jingdong Song / Jing Zheng / Hongrong Liu / |
PubMed 要旨 | T5 is a siphophage that has been extensively studied by structural and biochemical methods. However, the complete in situ structures of T5 before and after DNA ejection remain unknown. In this study, ...T5 is a siphophage that has been extensively studied by structural and biochemical methods. However, the complete in situ structures of T5 before and after DNA ejection remain unknown. In this study, we used cryo-electron microscopy (cryo-EM) to determine the structures of mature T5 (a laboratory-adapted, fiberless T5 mutant) and urea-treated empty T5 (lacking the tip complex) at near-atomic resolutions. Atomic models of the head, connector complex, tail tube, and tail tip were built for mature T5, and atomic models of the connector complex, comprising the portal protein pb7, adaptor protein p144, and tail terminator protein p142, were built for urea-treated empty T5. Our findings revealed that the aforementioned proteins did not undergo global conformational changes before and after DNA ejection, indicating that these structural features were conserved among most myophages and siphophages. The present study elucidates the underlying mechanisms of siphophage infection and DNA ejection. |
リンク | Int J Mol Sci / PubMed:39126049 / PubMed Central |
手法 | EM (単粒子) / EM (らせん対称) |
解像度 | 3.4 - 4.8 Å |
構造データ | EMDB-60511, PDB-8zvi: EMDB-60672, PDB-9ilp: EMDB-60675, PDB-9ilv: EMDB-60689, PDB-9imh: EMDB-60695, PDB-9imv: EMDB-60712, PDB-9iny: EMDB-60750, PDB-9ioz: |
由来 |
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キーワード | STRUCTURAL PROTEIN / Complex / VIRAL PROTEIN |