EMDB/PDB/SASBDBから引用されている文献のデータベース

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構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
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IF	>30 >20 >10 >5 all

タイトル	Structures of the Escherichia coli type 1 pilus during pilus rod assembly and after assembly termination.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 4988, Year 2025
掲載日	2025年5月29日
著者	Paul Bachmann / Pavel Afanasyev / Daniel Boehringer / Rudi Glockshuber /
PubMed 要旨	Uropathogenic Escherichia coli strains use filamentous type 1 pili to adhere to and invade uroepithelial cells. The pilus consists of a flexible tip fibrillum, formed by the adhesin FimH and the ...Uropathogenic Escherichia coli strains use filamentous type 1 pili to adhere to and invade uroepithelial cells. The pilus consists of a flexible tip fibrillum, formed by the adhesin FimH and the subunits FimG and FimF. The pilus rod is a helical assembly of up to 3000 copies of the main subunit FimA, terminated by a single copy of the subunit FimI that anchors the rod to the assembly platform FimD in the outer membrane. Although type 1 pilus assembly can be completely reconstituted in vitro, the precise mechanism of assembly termination on FimD is still unknown. Here, we present cryo-electron microscopy structures of the fully assembled pilus with all its components prior to and after incorporation of FimI, capped with the assembly chaperone FimC. The structures reveal that FimD positions the proximal end of the pilus rod at an angle of ca. 50 degrees relative to the plane of the outer membrane. Specific interactions between FimI and FimC, absent in the equivalent FimA-FimC interface of the non-terminated pilus, stabilize the assembly-terminated state. In addition, we present structures of the transition region between the tip fibrillum and the helical rod, showing how FimF aligns the tip fibrillum along the rod axis.
リンク	Nat Commun / PubMed:40442073 / PubMed Central
手法	EM (単粒子)
解像度	3.1 - 6.4 Å
構造データ	50751 9ftt EMDB-50751, PDB-9ftt: Cryo-EM structure of the type 1 pilus rod as part of the FimA-bound usher complex (FimDHGFAnC) 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-50755: Cryo-EM map of the type 1 pilus complex including pilus rod and FimA-bound assembly platform 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-50773: Cryo-EM map of the type 1 chaperone-usher pilus tip and rod - Conformer 1 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-50796: Cryo-EM map of the type 1 chaperone-usher pilus tip and rod - Conformer 2 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-50806: Cryo-EM map of the type 1 chaperone-usher pilus tip and rod - Conformer 3 手法: EM (単粒子) / 解像度: 4.4 Å EMDB-50809: Cryo-EM map of the type 1 chaperone-usher pilus tip and rod - Conformer 4 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-50810: Cryo-EM map of the type 1 pilus complex including pilus rod and FimI-bound assembly platform 手法: EM (単粒子) / 解像度: 6.4 Å EMDB-50812: Cryo-EM map of the type 1 pilus complex including pilus rod and FimI-bound assembly platform after incorporation of two FimI subunits 手法: EM (単粒子) / 解像度: 4.3 Å 50828 9fw9 EMDB-50828, PDB-9fw9: Cryo-EM structure of the type 1 pilus assembly platform as part of the FimA-bound chaperone-usher pilus complex (FimDHGFAnC - body 2) 手法: EM (単粒子) / 解像度: 3.9 Å 50829 9fwb EMDB-50829, PDB-9fwb: Cryo-EM structure of the type 1 pilus assembly platform as part of the FimA-bound chaperone-usher pilus complex (Local refinement including FimD, FimC, FimAn and FimAn-1) 手法: EM (単粒子) / 解像度: 3.5 Å 50839 9fx0 EMDB-50839, PDB-9fx0: Cryo-EM structure of the type 1 pilus tip-to-rod transition 手法: EM (単粒子) / 解像度: 3.1 Å 50843 9fx8 EMDB-50843, PDB-9fx8: Cryo-EM structure of the FimI-bound type 1 pilus assembly platform complex - Local refinement 手法: EM (単粒子) / 解像度: 3.6 Å 50846 9fxa EMDB-50846, PDB-9fxa: Cryo-EM structure of the type 1 pilus rod as part of the FimI-bound usher complex (FimDHGFAnIC - body 1) 手法: EM (単粒子) / 解像度: 4.0 Å 50847 9fxb EMDB-50847, PDB-9fxb: Cryo-EM structure of the type 1 pilus assembly platform as part of the FimI-bound chaperone-usher pilus complex (FimDHGFAnIC - body 2) 手法: EM (単粒子) / 解像度: 4.3 Å 50853 9fxs EMDB-50853, PDB-9fxs: Cryo-EM structure of the type 1 pilus complex including pilus rod and FimI-bound assembly platform after incorporation of two FimI subunits - Local refinement 手法: EM (単粒子) / 解像度: 4.2 Å 50861 9fy9 EMDB-50861, PDB-9fy9: Cryo-EM structure of the type 1 chaperone-usher pilus FimD-tip complex (FimDHGFC) - Conformer 1 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-50954: Cryo-EM map of the type 1 chaperone-usher pilus FimD-tip complex - Conformer 2 手法: EM (単粒子) / 解像度: 4.2 Å
由来	escherichia coli (大腸菌)
キーワード	CELL ADHESION / chaperone / usher / pilus / rod / MEMBRANE PROTEIN / tip / termination