EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural and mechanistic insights into herpesvirus helicase-primase and its therapeutic inhibitors.
ジャーナル・号・ページ	Nat Microbiol, Vol. 10, Issue 12, Page 3191-3201, Year 2025
掲載日	2025年11月4日
著者	Qing Yao / Alexandre Mercier / Arabinda Nayak / Lindsey May / Pui Yan Ho / Ariel Lewis-Ballester / Varsha Nair / Annapurna Sapre / Thomas Aeschbacher / Jit Mukherjee / Christopher Richards / Roberto Mateo / Aesop Cho / Eric Lansdon / Xinchao Yu /
PubMed 要旨	The herpes simplex virus (HSV) helicase-primase (HP) complex is a promising anti-herpes therapeutic target. However, progress in developing highly effective small-molecule HP inhibitors (HPIs) for ...The herpes simplex virus (HSV) helicase-primase (HP) complex is a promising anti-herpes therapeutic target. However, progress in developing highly effective small-molecule HP inhibitors (HPIs) for the treatment of genital herpes has been hindered by the lack of structural information on the HP complex and the incomplete understanding of the mechanism of action of HPIs. Here we present the cryogenic electron microscopy structure of the HSV-1 HP apo-complex (3.8 Å), along with structures bound to pritelivir (3.2 Å) and amenamevir (3.2 Å)-two clinically active, chemically distinct HPIs. The potency of both inhibitors against HSV variants bearing mutations within the HPI binding pocket supports the high-resolution mapping of key molecular interactions while revealing residues that govern their antiviral spectrum against alphaherpesviruses. Our results provide important insight into the unique architecture of the HP complex and the mechanism of inhibition of HPIs, paving the way for the development of next-generation antivirals to treat herpesvirus infections.
リンク	Nat Microbiol / PubMed:41188384 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 3.8 Å
構造データ	49269 9nda EMDB-49269, PDB-9nda: The rigid portion of Cryo-EM structure of Herpesvirus Helicase-Primase complex with amenamevir 手法: EM (単粒子) / 解像度: 2.9 Å 49276 9ndq EMDB-49276, PDB-9ndq: The rigid portion of Cryo-EM structure of Herpesvirus Helicase-Primase complex prepared with forked DNA and ATP-gamma-S 手法: EM (単粒子) / 解像度: 3.0 Å 49277 9ndt EMDB-49277, PDB-9ndt: The flexible portion of Cryo-EM structure of Herpesvirus Helicase-Primase complex prepared with forked DNA and ATP-gamma-S 手法: EM (単粒子) / 解像度: 3.8 Å 49290 9ndz EMDB-49290, PDB-9ndz: The rigid portion of Cryo-EM structure of Herpesvirus Helicase-Primase complex prepared with forked DNA, ATP-gamma-S and Pritelivir 手法: EM (単粒子) / 解像度: 3.0 Å 49291 9ne0 EMDB-49291, PDB-9ne0: The flexible portion of Cryo-EM structure of Herpesvirus Helicase-Primase complex prepared with forked DNA, ATP-gamma-S and Pritelivir 手法: EM (単粒子) / 解像度: 3.4 Å 49304 9neb EMDB-49304, PDB-9neb: The rigid portion of Cryo-EM structure of Herpesvirus Helicase-Primase complex with Pritelivir 手法: EM (単粒子) / 解像度: 2.8 Å 49306 9nee EMDB-49306, PDB-9nee: The flexible portion of Cryo-EM structure of Herpesvirus Helicase-Primase complex with Pritelivir 手法: EM (単粒子) / 解像度: 3.2 Å 49326 9nel EMDB-49326, PDB-9nel: The flexible portion of Cryo-EM structure of Herpesvirus Helicase-Primase complex with amenamevir 手法: EM (単粒子) / 解像度: 3.2 Å
化合物	PDB-1bxb: XYLOSE ISOMERASE FROM THERMUS THERMOPHILUS PDB-1bxd: NMR STRUCTURE OF THE HISTIDINE KINASE DOMAIN OF THE E. COLI OSMOSENSOR ENVZ
由来	herpesviridae (ウイルス)
キーワード	REPLICATION / TRANSFERASE / Inhibitor / Helicase / Primase / Herpesvirus / Forked DNA / Pritelivir / TRANSFERASE/INHIBITOR / TRANSFERASE-INHIBITOR complex