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-Structure paper
タイトル | Structure and genome ejection mechanism of phage P68. |
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ジャーナル・号・ページ | Sci Adv, Vol. 5, Issue 10, Page eaaw7414, Year 2019 |
掲載日 | 2019年10月16日 |
著者 | Dominik Hrebík / Dana Štveráková / Karel Škubník / Tibor Füzik / Roman Pantůček / Pavel Plevka / |
PubMed 要旨 | Phages infecting can be used as therapeutics against antibiotic-resistant bacterial infections. However, there is limited information about the mechanism of genome delivery of phages that infect ...Phages infecting can be used as therapeutics against antibiotic-resistant bacterial infections. However, there is limited information about the mechanism of genome delivery of phages that infect Gram-positive bacteria. Here, we present the structures of native phage P68, genome ejection intermediate, and empty particle. The P68 head contains 72 subunits of inner core protein, 15 of which bind to and alter the structure of adjacent major capsid proteins and thus specify attachment sites for head fibers. Unlike in the previously studied phages, the head fibers of P68 enable its virion to position itself at the cell surface for genome delivery. The unique interaction of one end of P68 DNA with one of the 12 portal protein subunits is disrupted before the genome ejection. The inner core proteins are released together with the DNA and enable the translocation of phage genome across the bacterial membrane into the cytoplasm. |
リンク | Sci Adv / PubMed:31663016 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 2 - 22.7 Å |
構造データ | EMDB-4436: EMDB-4437: EMDB-4440, PDB-6iaw: EMDB-4442, PDB-6ib1: EMDB-4449: EMDB-4450: EMDB-4451: EMDB-4453: EMDB-4454: EMDB-4455: EMDB-4456: EMDB-4457: EMDB-4458: PDB-6iab: |
化合物 | ChemComp-HOH: |
由来 |
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キーワード | SUGAR BINDING PROTEIN / structural protein / receptor binding protein / beta-propeller / structural proteins / bacteriophage portal / bacteriophage tail / native complex / bacteriophage capsid / HK97 fold / bacteriophage / head fiber / inner core protein / VIRUS / complex |