EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Coupling enzymatic activity and gating in an ancient TRPM chanzyme and its molecular evolution.
ジャーナル・号・ページ	Nat Struct Mol Biol, Year 2024
掲載日	2024年5月21日
著者	Yihe Huang / Sushant Kumar / Junuk Lee / Wei Lü / Juan Du /
PubMed 要旨	Channel enzymes represent a class of ion channels with enzymatic activity directly or indirectly linked to their channel function. We investigated a TRPM2 chanzyme from choanoflagellates that ...Channel enzymes represent a class of ion channels with enzymatic activity directly or indirectly linked to their channel function. We investigated a TRPM2 chanzyme from choanoflagellates that integrates two seemingly incompatible functions into a single peptide: a channel module activated by ADP-ribose with high open probability and an enzyme module (NUDT9-H domain) consuming ADP-ribose at a remarkably slow rate. Using time-resolved cryogenic-electron microscopy, we captured a complete series of structural snapshots of gating and catalytic cycles, revealing the coupling mechanism between channel gating and enzymatic activity. The slow kinetics of the NUDT9-H enzyme module confers a self-regulatory mechanism: ADPR binding triggers NUDT9-H tetramerization, promoting channel opening, while subsequent hydrolysis reduces local ADPR, inducing channel closure. We further demonstrated how the NUDT9-H domain has evolved from a structurally semi-independent ADP-ribose hydrolase module in early species to a fully integrated component of a gating ring essential for channel activation in advanced species.
リンク	Nat Struct Mol Biol / PubMed:38773335
手法	EM (単粒子)
解像度	1.97 - 4.08 Å
構造データ	40721 8sr7 EMDB-40721, PDB-8sr7: Cryo-EM structure of TRPM2 chanzyme in the presence of Magnesium, Adenosine monophosphate, and Ribose-5-phosphate 手法: EM (単粒子) / 解像度: 1.97 Å 40722 8sr8 EMDB-40722, PDB-8sr8: Cryo-EM structure of TRPM2 chanzyme in the presence of EDTA (apo state) 手法: EM (単粒子) / 解像度: 2.77 Å 40723 8sr9 EMDB-40723, PDB-8sr9: Cryo-EM structure of TRPM2 chanzyme in the presence of Magnesium 手法: EM (単粒子) / 解像度: 3.05 Å 40724 8sra EMDB-40724, PDB-8sra: Cryo-EM structure of TRPM2 chanzyme in the presence of Calcium 手法: EM (単粒子) / 解像度: 2.93 Å 40725 8srb EMDB-40725, PDB-8srb: Cryo-EM structure of TRPM2 chanzyme in the presence of EDTA and ADP-ribose 手法: EM (単粒子) / 解像度: 2.82 Å 40726 8src EMDB-40726, PDB-8src: Cryo-EM structure of TRPM2 chanzyme in the presence of Calcium and ADP-ribose 手法: EM (単粒子) / 解像度: 2.92 Å EMDB-40865: NUDT9-H domain focused cryo-EM map of TRPM2 chanzyme in the presence of Calcium and ADP-ribose 手法: EM (単粒子) / 解像度: 3.69 Å EMDB-40866: NUDT9-H domain focused cryo-EM map of TRPM2 chanzyme in the presence of Magnesium, Adenosine monophosphate, and Ribose-5-phosphate 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-40867: NUDT9-H domain focused cryo-EM map of TRPM2 chanzyme in the presence of Magnesium, Adenosine monophosphate, and Ribose-5-phosphate 手法: EM (単粒子) / 解像度: 3.83 Å EMDB-40868: NUDT9-H domain focused cryo-EM map of TRPM2 chanzyme in the presence of Magnesium and ADP-ribose, open state 手法: EM (単粒子) / 解像度: 3.13 Å EMDB-40869: NUDT9-H domain focused cryo-EM map of TRPM2 chanzyme in the presence of Magnesium and ADP-ribose, closed state 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-40870: NUDT9-H domain focused cryo-EM map of TRPM2 chanzyme in the presence of Magnesium, ADP-ribose, Adenosine monophosphate, and Ribose-5-phosphate, closed state 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-40871: NUDT9-H domain focused cryo-EM map of TRPM2 chanzyme (E1114A) in the presence of Magnesium and ADP-ribose, open state 手法: EM (単粒子) / 解像度: 4.08 Å EMDB-40872: NUDT9-H domain focused cryo-EM map of TRPM2 chanzyme (E1114A) in the presence of Magnesium and ADP-ribose, closed state 手法: EM (単粒子) / 解像度: 3.27 Å EMDB-40875: Raw consensus map of TRPM2 chanzyme in the presence of Magnesium, Adenosine monophosphate, and Ribose-5-phosphate 手法: EM (単粒子) / 解像度: 1.97 Å EMDB-40876: Raw consensus map of TRPM2 chanzyme in the presence of Magnesium 手法: EM (単粒子) / 解像度: 3.16 Å EMDB-40877: Raw consensus map of TRPM2 chanzyme in the presence of Calcium 手法: EM (単粒子) / 解像度: 3.03 Å EMDB-40878: Raw consensus map of TRPM2 chanzyme in the presence of EDTA and ADP-ribose 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-40879: Raw consensus map of TRPM2 chanzyme in the presence of Magnesium and ADP-ribose, open state 手法: EM (単粒子) / 解像度: 2.93 Å EMDB-40880: Raw consensus map of TRPM2 chanzyme in the presence of Magnesium and ADP-ribose, closed state 手法: EM (単粒子) / 解像度: 3.87 Å EMDB-40881: Raw consensus map of TRPM2 chanzyme in the presence of Magnesium, ADP-ribose, Adenosine monophosphate, and Ribose-5-phosphate, closed state 手法: EM (単粒子) / 解像度: 3.71 Å EMDB-40883: Raw consensus map of TRPM2 chanzyme in the presence of Magnesium, Adenosine monophosphate, and Ribose-5-phosphate 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-40887: Raw consensus map of TRPM2 chanzyme (E1114A) in the presence of Magnesium and ADP-ribose, open state 手法: EM (単粒子) / 解像度: 3.87 Å EMDB-40888: Raw consensus map of TRPM2 chanzyme (E1114A) in the presence of Magnesium and ADP-ribose, closed state 手法: EM (単粒子) / 解像度: 2.82 Å EMDB-40893: Raw consensus map of TRPM2 chanzyme in the presence of Calcium and ADP-ribose. 手法: EM (単粒子) / 解像度: 2.99 Å EMDB-40895: Raw consensus map of TRPM2 chanzyme in the presence of EDTA (apo state) 手法: EM (単粒子) / 解像度: 3.02 Å
化合物	ChemComp-RP5: 5-O-phosphono-beta-D-ribofuranose / β-D-リボフラノ-ス5-りん酸 ChemComp-AMP: ADENOSINE MONOPHOSPHATE / AMP / AMPYM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-CLR: CHOLESTEROL / コレステロ-ル ChemComp-HOH: WATER ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-APR*: ADENOSINE-5-DIPHOSPHORIBOSE
由来	salpingoeca rosetta (真核生物)
キーワード	TRANSPORT PROTEIN / TRPM2 Chanzyme / Channel-enzyme