EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insights into autoinhibition and activation of defense-associated sirtuin protein.
ジャーナル・号・ページ	Int J Biol Macromol, Vol. 277, Issue Pt 1, Page 134145, Year 2024
掲載日	2024年7月24日
著者	Xu Yang / Yiqun Wang / Jianting Zheng /
PubMed 要旨	Bacterial defense-associated sirtuin 2 (DSR2) proteins harbor an N-terminal sirtuin (SIR2) domain degrading NAD. DSR2 from Bacillus subtilis 29R is autoinhibited and unable to hydrolyze NAD in the ...Bacterial defense-associated sirtuin 2 (DSR2) proteins harbor an N-terminal sirtuin (SIR2) domain degrading NAD. DSR2 from Bacillus subtilis 29R is autoinhibited and unable to hydrolyze NAD in the absence of phage infection. A tail tube protein (TTP) of phage SPR activates the DSR2 while a DSR2-inhibiting protein of phage SPbeta, known as DSAD1 (DSR anti-defense 1), inactivates the DSR2. Although DSR2 structures in complexed with TTP and DSAD1, respectively, have been reported recently, the autoinhibition and activation mechanisms remain incompletely understood. Here, we present cryo-electron microscopy structures of the DSR2-NAD complex in autoinhibited state and the in vitro assembled DSR2-TFD (TTP tube-forming domain) complex in activated state. The DSR2-NAD complex reveals that the autoinhibited DSR2 assembles into an inactive tetramer, binding NAD through a distinct pocket situated outside active site. Binding of TFD into cavities within the sensor domains of DSR2 triggers a conformational change in SIR2 regions, activating its NADase activity, whereas the TTP β-sandwich domain (BSD) is flexible and does not contribute to the activation process. The activated form of DSR2 exists as tetramers and dimers, with the tetramers exhibiting more NADase activity. Overall, our results extend the current understanding of autoinhibition and activation of DSR2 immune proteins.
リンク	Int J Biol Macromol / PubMed:39059542
手法	EM (単粒子)
解像度	2.9 - 9.74 Å
構造データ	39369 EMDBエントリ画像なし 8ykf EMDB-39369, PDB-8ykf: The DSR2-DSAD1 complex with DSAD1 on the opposite sides 手法: EM (単粒子) / 解像度: 3.35 Å EMDBエントリ画像なし EMDB-39380: Local map of DSR2-DSAD1 complex 手法: EM (単粒子) / 解像度: 2.9 Å EMDBエントリ画像なし EMDB-39381: Overall map of DSR2-DSAD1 complex 手法: EM (単粒子) / 解像度: 2.98 Å 39382 EMDBエントリ画像なし 8yl5 EMDB-39382, PDB-8yl5: The DSR2-DSAD1 complex with DSAD1 on the same sides 手法: EM (単粒子) / 解像度: 3.45 Å 39385 EMDBエントリ画像なし 8yln EMDB-39385, PDB-8yln: The structure of DSR2-Tail tube complex 手法: EM (単粒子) / 解像度: 3.53 Å EMDBエントリ画像なし EMDB-39386: The loacl refined map of DSR2 and NAD structure 手法: EM (単粒子) / 解像度: 9.74 Å EMDBエントリ画像なし EMDB-39387: The local refined map of DSR2 N-terminal domains 手法: EM (単粒子) / 解像度: 2.92 Å 39390 EMDBエントリ画像なし 8ylt EMDB-39390, PDB-8ylt: The structure of DSR2 and NAD+ complex 手法: EM (単粒子) / 解像度: 3.09 Å 39718 EMDBエントリ画像なし 8z18 EMDB-39718, PDB-8z18: The tetramer complex of DSR2 and tube-forming domain of phage tail tube protein 手法: EM (単粒子) / 解像度: 3.94 Å 60470 EMDBエントリ画像なし 8ztr EMDB-60470, PDB-8ztr: The dimer complex of DSR2 and tube-forming domain of phage tail tube protein 手法: EM (単粒子) / 解像度: 3.26 Å
化合物	ChemComp-NAD: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM
由来	bacillus subtilis (枯草菌) bacillus phage spbeta (ファージ) bacillus phage spr (ファージ) bacillus subtilis subsp. natto (strain best195) (納豆菌) bacillus subtilis subsp. natto best195 (納豆菌)
キーワード	IMMUNE SYSTEM / Complex